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The map label for this gene is mudD [H]

Identifier: 89897960

GI number: 89897960

Start: 184891

End: 187326

Strand: Direct

Name: mudD [H]

Synonym: CF0153

Alternate gene names: 89897960

Gene position: 184891-187326 (Clockwise)

Preceding gene: 89897959

Following gene: 89897964

Centisome position: 15.85

GC content: 38.67

Gene sequence:

>2436_bases
ATGGATAGGAAAATCCACTATCATTTTATAGGTATCGGTGGAATAGGAATGAGTGCATTAGCTCATATTTTATTAGACCG
GGGATATTCCGTATCGGGGAGTGATTTAAATCAAGGTGCTACTGTGGATCAACTGGTTGCCAAAGGAGCAATATTTTTAC
CAGGGCACAAGGAAAATCATGTTCCTGAAGATTGCACTATTATTTACGGATCAGGAATTTCTACAGATAACGTAGAGTAT
AAAGAAGCTGTAAGAAAACAACTTCCTATGATGCATCGTGCTGAGCTGCTTGCATTTTTAATGCAAGAGCATACGAGTAT
TTTAGTTTCAGGAAGTCATGGAAAAACTACGGTAACCTCATTAATTACAGCGATTTTTCAAACAGCGAAAAAAGATCCTT
CCTATGCGATTGGAGGGCTGAATTCTCTATCGTTAAATGGTTATTCAGGAAACTCAGAATACTTCATAGCAGAAGCTGAT
GAAAGCGATGGTTCGTTGAAATACTATCATCCTAAAATTGCCGTGGTGACAAATTTAGATAATGAGCACCTAAGTAATTT
TGAAGGGAATAAGGAAAAGCTAGCCTTAACGATCGAAGAATTTGCCCGTAAGGTTGATAATCCAAATTTATGCTTTTACA
ATGGCGATTGCCAAGAACTGAAGGGAAGGATCTCTGGAAAATCTTATGGATTCTCTCAAGATTGTCACCTGTATATTTAC
TCCCATCGTCAGGATGGATGGCGCTCCGTGTTTTCCCTATCTTTTTTAGAGAAAGATTACCAAGATATAGATCTTAACCT
TATAGGTAAGCACAATGTGGCAAATGCTGCTGTAGCTATGGGCATAGCATTAACTCTAGGGATCGATGAGGAAAGTATTC
GAGAAGCGCTTAAGAACTTTCCCGGAGTTCAAAGACGTATGGAGAGAAAGAATACTTCTGAAACATTCTTATTCCTTGAA
GACTATGCTCATCATCCTTCGGAGATTTCCTGCACCCTAGGGGGATTGCGGGATGCCGTGGGAGTGCGTCGTGTGATTGC
TATATGTCAACCTCATAGATTTTCTCGATTACAACATTGCCTAGAAGATTTTTTTAATGCCTTCCAAGATGCTGATGAGG
TAATTCTTACCGATATTTACAGTGCTGGAGAAACCCCTCTTGATTTGCCATCTCCTGAGAAATTAGCAGAAACGATTTCT
TTATCCTCTCATGTGCGCTGTAGCTATGTGCCTTATGACAATATTATTGGATATCTAAAACAAATAATCCGTGTTCATGA
TGTTTGCGTGTCTTTGGGGGCAGGGAATATTTATTCTGTAGGAAATGCTTTAAAGGACTTTGAACCTAAGAAACTTTCTG
TTGGTGTAGTTTGTGGAGGCCAGTCTTGTGAGCATAAAGTTTCTTTGTTGTCTGCAAAAAATATCATCCAATATCTTGCT
TCTGAGTACTATGATGTGCAGTACTTTGTTATCAATCGTCAAGGACTCTGGTCGAAAGTTACGGCTATTGATGCAGAACA
TGATTATGATAAGGAAGGTTATCATGTATTATCCTCAGAAATTGCAGAATCTCTAGCAAATGTAGATGTTATTCTTCCTA
TTTTACATGGCCCCTTCGGTGAAGACGGTACTCTTCAAGGATTCTTAGAAATTATCGATAAACCTTATGGTGGTCCTTCT
TTGCTTTTCTCTGCCATAAGTATGGATAAAGTCATGACAAAACGTCTTGCAGCTTCTATAGGCGTACCTGTGGTTCCTTA
TCAACCGTTAACTTTGCATGCTTGGAAAAGAACTCCAGACTTATGCATGCGTAGGATTTTAGAGACCTTTACTTTTCCTA
TGTTTGTTAAAGCAGCACATTTAGGATCAAGTATCGGGGTATTCGAAGTTCATAACGAGGAAGAGCTAAAATCCAAGATT
TCCGAAGCTTTCCAATGCGATACCGATATCTTTATTGAAGAGAGCCGTTTAGGATCTCGGGAAATTGAAGTGTCTTGTCT
TGGAGATGCAAGTAGCAGTTATTATATTTCCGAACCACACGAGCGTTGTGGGGAGAAAAGATTCATTAGCTACGAAGAGA
AATACGGATTCAATGGTAAATCTAGTGCGAAAATACAATACGATTTAAACTTCTCCAAAGAAGCTAAAGCAAGAGTGAAA
GAACTTACAGAGCGTATATATCGTGTTATCCAGGGAAAAGGTTCTTGTAGAATCGATTTCTTTTTAGATAATGAAGGAGA
GTTCTGGCTTTCTGAAATAAATCCTATTCCAGGAATGACCGAGTCTAGCCCATTTTTACATGATTTTGTTCGTGTAGGAT
GGACCTTTGAACAGATCATGCATCAATTGATTATATCGGGATTACATAAGTTTGATCAAAAGAAGAAAATTCACAGTACT
TTTGCTAAGCAACGCTCATTAATCGCGAAGAGCTAA

Upstream 100 bases:

>100_bases
AAAACTATTAAAAATAGGCGAGAAGCCTTGCGAGCTTATTATCAAAACAAATCCTCAAAGTCTTTTTATCAATTTATATG
TGAATGTTTATAGGTAATCT

Downstream 100 bases:

>100_bases
AAACTCCGCAAAGGGGTTTTTAGCTATTGCCAAATTCTGTTTATAAAGATGTGGAAAAGTAAGTGTGTTATTCAGAATGG
TGATTGTGGCTGCATTTATG

Product: bifunctional D-alanyl-alanine synthetase A/UDP-N-acetylmuramate--L-alanine ligase

Products: NA

Alternate protein names: UDP-N-acetylmuramate--L-alanine ligase; UDP-N-acetylmuramoyl-L-alanine synthetase; D-alanine--D-alanine ligase; D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 811; Mature: 811

Protein sequence:

>811_residues
MDRKIHYHFIGIGGIGMSALAHILLDRGYSVSGSDLNQGATVDQLVAKGAIFLPGHKENHVPEDCTIIYGSGISTDNVEY
KEAVRKQLPMMHRAELLAFLMQEHTSILVSGSHGKTTVTSLITAIFQTAKKDPSYAIGGLNSLSLNGYSGNSEYFIAEAD
ESDGSLKYYHPKIAVVTNLDNEHLSNFEGNKEKLALTIEEFARKVDNPNLCFYNGDCQELKGRISGKSYGFSQDCHLYIY
SHRQDGWRSVFSLSFLEKDYQDIDLNLIGKHNVANAAVAMGIALTLGIDEESIREALKNFPGVQRRMERKNTSETFLFLE
DYAHHPSEISCTLGGLRDAVGVRRVIAICQPHRFSRLQHCLEDFFNAFQDADEVILTDIYSAGETPLDLPSPEKLAETIS
LSSHVRCSYVPYDNIIGYLKQIIRVHDVCVSLGAGNIYSVGNALKDFEPKKLSVGVVCGGQSCEHKVSLLSAKNIIQYLA
SEYYDVQYFVINRQGLWSKVTAIDAEHDYDKEGYHVLSSEIAESLANVDVILPILHGPFGEDGTLQGFLEIIDKPYGGPS
LLFSAISMDKVMTKRLAASIGVPVVPYQPLTLHAWKRTPDLCMRRILETFTFPMFVKAAHLGSSIGVFEVHNEEELKSKI
SEAFQCDTDIFIEESRLGSREIEVSCLGDASSSYYISEPHERCGEKRFISYEEKYGFNGKSSAKIQYDLNFSKEAKARVK
ELTERIYRVIQGKGSCRIDFFLDNEGEFWLSEINPIPGMTESSPFLHDFVRVGWTFEQIMHQLIISGLHKFDQKKKIHST
FAKQRSLIAKS

Sequences:

>Translated_811_residues
MDRKIHYHFIGIGGIGMSALAHILLDRGYSVSGSDLNQGATVDQLVAKGAIFLPGHKENHVPEDCTIIYGSGISTDNVEY
KEAVRKQLPMMHRAELLAFLMQEHTSILVSGSHGKTTVTSLITAIFQTAKKDPSYAIGGLNSLSLNGYSGNSEYFIAEAD
ESDGSLKYYHPKIAVVTNLDNEHLSNFEGNKEKLALTIEEFARKVDNPNLCFYNGDCQELKGRISGKSYGFSQDCHLYIY
SHRQDGWRSVFSLSFLEKDYQDIDLNLIGKHNVANAAVAMGIALTLGIDEESIREALKNFPGVQRRMERKNTSETFLFLE
DYAHHPSEISCTLGGLRDAVGVRRVIAICQPHRFSRLQHCLEDFFNAFQDADEVILTDIYSAGETPLDLPSPEKLAETIS
LSSHVRCSYVPYDNIIGYLKQIIRVHDVCVSLGAGNIYSVGNALKDFEPKKLSVGVVCGGQSCEHKVSLLSAKNIIQYLA
SEYYDVQYFVINRQGLWSKVTAIDAEHDYDKEGYHVLSSEIAESLANVDVILPILHGPFGEDGTLQGFLEIIDKPYGGPS
LLFSAISMDKVMTKRLAASIGVPVVPYQPLTLHAWKRTPDLCMRRILETFTFPMFVKAAHLGSSIGVFEVHNEEELKSKI
SEAFQCDTDIFIEESRLGSREIEVSCLGDASSSYYISEPHERCGEKRFISYEEKYGFNGKSSAKIQYDLNFSKEAKARVK
ELTERIYRVIQGKGSCRIDFFLDNEGEFWLSEINPIPGMTESSPFLHDFVRVGWTFEQIMHQLIISGLHKFDQKKKIHST
FAKQRSLIAKS
>Mature_811_residues
MDRKIHYHFIGIGGIGMSALAHILLDRGYSVSGSDLNQGATVDQLVAKGAIFLPGHKENHVPEDCTIIYGSGISTDNVEY
KEAVRKQLPMMHRAELLAFLMQEHTSILVSGSHGKTTVTSLITAIFQTAKKDPSYAIGGLNSLSLNGYSGNSEYFIAEAD
ESDGSLKYYHPKIAVVTNLDNEHLSNFEGNKEKLALTIEEFARKVDNPNLCFYNGDCQELKGRISGKSYGFSQDCHLYIY
SHRQDGWRSVFSLSFLEKDYQDIDLNLIGKHNVANAAVAMGIALTLGIDEESIREALKNFPGVQRRMERKNTSETFLFLE
DYAHHPSEISCTLGGLRDAVGVRRVIAICQPHRFSRLQHCLEDFFNAFQDADEVILTDIYSAGETPLDLPSPEKLAETIS
LSSHVRCSYVPYDNIIGYLKQIIRVHDVCVSLGAGNIYSVGNALKDFEPKKLSVGVVCGGQSCEHKVSLLSAKNIIQYLA
SEYYDVQYFVINRQGLWSKVTAIDAEHDYDKEGYHVLSSEIAESLANVDVILPILHGPFGEDGTLQGFLEIIDKPYGGPS
LLFSAISMDKVMTKRLAASIGVPVVPYQPLTLHAWKRTPDLCMRRILETFTFPMFVKAAHLGSSIGVFEVHNEEELKSKI
SEAFQCDTDIFIEESRLGSREIEVSCLGDASSSYYISEPHERCGEKRFISYEEKYGFNGKSSAKIQYDLNFSKEAKARVK
ELTERIYRVIQGKGSCRIDFFLDNEGEFWLSEINPIPGMTESSPFLHDFVRVGWTFEQIMHQLIISGLHKFDQKKKIHST
FAKQRSLIAKS

Specific function: Cell Wall Formation. [C]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786279, Length=466, Percent_Identity=33.0472103004292, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1786579, Length=375, Percent_Identity=30.4, Blast_Score=174, Evalue=3e-44,
Organism=Escherichia coli, GI1790680, Length=389, Percent_Identity=25.1928020565553, Blast_Score=132, Evalue=1e-31,
Organism=Escherichia coli, GI1786280, Length=335, Percent_Identity=31.3432835820896, Blast_Score=122, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR016040
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005758 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.8; =6.3.2.4 [H]

Molecular weight: Translated: 90800; Mature: 90800

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRKIHYHFIGIGGIGMSALAHILLDRGYSVSGSDLNQGATVDQLVAKGAIFLPGHKENH
CCCEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCCC
VPEDCTIIYGSGISTDNVEYKEAVRKQLPMMHRAELLAFLMQEHTSILVSGSHGKTTVTS
CCCCCEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHH
LITAIFQTAKKDPSYAIGGLNSLSLNGYSGNSEYFIAEADESDGSLKYYHPKIAVVTNLD
HHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCEEEEECCCCCCCEEEECCEEEEEECCC
NEHLSNFEGNKEKLALTIEEFARKVDNPNLCFYNGDCQELKGRISGKSYGFSQDCHLYIY
HHHHCCCCCCCHHEEEEHHHHHHHCCCCCEEEECCCHHHHHHCCCCCCCCCCCCCEEEEE
SHRQDGWRSVFSLSFLEKDYQDIDLNLIGKHNVANAAVAMGIALTLGIDEESIREALKNF
ECCCCHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHEEEEECCCHHHHHHHHHHC
PGVQRRMERKNTSETFLFLEDYAHHPSEISCTLGGLRDAVGVRRVIAICQPHRFSRLQHC
CHHHHHHHHCCCCCEEEEEEHHCCCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LEDFFNAFQDADEVILTDIYSAGETPLDLPSPEKLAETISLSSHVRCSYVPYDNIIGYLK
HHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHH
QIIRVHDVCVSLGAGNIYSVGNALKDFEPKKLSVGVVCGGQSCEHKVSLLSAKNIIQYLA
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
SEYYDVQYFVINRQGLWSKVTAIDAEHDYDKEGYHVLSSEIAESLANVDVILPILHGPFG
HHCCEEEEEEEECCCCCHHHEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCC
EDGTLQGFLEIIDKPYGGPSLLFSAISMDKVMTKRLAASIGVPVVPYQPLTLHAWKRTPD
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHH
LCMRRILETFTFPMFVKAAHLGSSIGVFEVHNEEELKSKISEAFQCDTDIFIEESRLGSR
HHHHHHHHHHCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEECCCCCCC
EIEVSCLGDASSSYYISEPHERCGEKRFISYEEKYGFNGKSSAKIQYDLNFSKEAKARVK
EEEEEEECCCCCCCEECCHHHHCCCCCCCCHHHHCCCCCCCCCEEEEECCCCHHHHHHHH
ELTERIYRVIQGKGSCRIDFFLDNEGEFWLSEINPIPGMTESSPFLHDFVRVGWTFEQIM
HHHHHHHHHHCCCCCEEEEEEECCCCCEEHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHH
HQLIISGLHKFDQKKKIHSTFAKQRSLIAKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDRKIHYHFIGIGGIGMSALAHILLDRGYSVSGSDLNQGATVDQLVAKGAIFLPGHKENH
CCCEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCCC
VPEDCTIIYGSGISTDNVEYKEAVRKQLPMMHRAELLAFLMQEHTSILVSGSHGKTTVTS
CCCCCEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHH
LITAIFQTAKKDPSYAIGGLNSLSLNGYSGNSEYFIAEADESDGSLKYYHPKIAVVTNLD
HHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCEEEEECCCCCCCEEEECCEEEEEECCC
NEHLSNFEGNKEKLALTIEEFARKVDNPNLCFYNGDCQELKGRISGKSYGFSQDCHLYIY
HHHHCCCCCCCHHEEEEHHHHHHHCCCCCEEEECCCHHHHHHCCCCCCCCCCCCCEEEEE
SHRQDGWRSVFSLSFLEKDYQDIDLNLIGKHNVANAAVAMGIALTLGIDEESIREALKNF
ECCCCHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHEEEEECCCHHHHHHHHHHC
PGVQRRMERKNTSETFLFLEDYAHHPSEISCTLGGLRDAVGVRRVIAICQPHRFSRLQHC
CHHHHHHHHCCCCCEEEEEEHHCCCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LEDFFNAFQDADEVILTDIYSAGETPLDLPSPEKLAETISLSSHVRCSYVPYDNIIGYLK
HHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHH
QIIRVHDVCVSLGAGNIYSVGNALKDFEPKKLSVGVVCGGQSCEHKVSLLSAKNIIQYLA
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
SEYYDVQYFVINRQGLWSKVTAIDAEHDYDKEGYHVLSSEIAESLANVDVILPILHGPFG
HHCCEEEEEEEECCCCCHHHEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCC
EDGTLQGFLEIIDKPYGGPSLLFSAISMDKVMTKRLAASIGVPVVPYQPLTLHAWKRTPD
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHH
LCMRRILETFTFPMFVKAAHLGSSIGVFEVHNEEELKSKISEAFQCDTDIFIEESRLGSR
HHHHHHHHHHCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEECCCCCCC
EIEVSCLGDASSSYYISEPHERCGEKRFISYEEKYGFNGKSSAKIQYDLNFSKEAKARVK
EEEEEEECCCCCCCEECCHHHHCCCCCCCCHHHHCCCCCCCCCEEEEECCCCHHHHHHHH
ELTERIYRVIQGKGSCRIDFFLDNEGEFWLSEINPIPGMTESSPFLHDFVRVGWTFEQIM
HHHHHHHHHHCCCCCEEEEEEECCCCCEEHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHH
HQLIISGLHKFDQKKKIHSTFAKQRSLIAKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12682364 [H]