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The map label for this gene is dagA1 [C]

Identifier: 89897930

GI number: 89897930

Start: 141027

End: 142403

Strand: Direct

Name: dagA1 [C]

Synonym: CF0123

Alternate gene names: 89897930

Gene position: 141027-142403 (Clockwise)

Preceding gene: 89897929

Following gene: 89897931

Centisome position: 12.09

GC content: 43.06

Gene sequence:

>1377_bases
ATGTTACATTTTCTAGAACAACTAAATAATTTCTGCATTTCCTTCTGCGTATTCCCCATGATTTTATTTCTTGGTGGGCT
ATTAACATGGAAATTACGAGGTCTACAATTTACAGGGTTAAAGCTCGGCTTTGATCTCATGTTAAAAAATAAGCAGGAGA
GTTCATCTACTGTAGAGGGTAAGGTATCTCGTTATGAAGCCGTGGCCGGTATACTTGCTGGGAACTTTGGTACGGGGAAT
ATTGCGGGGATGGCTATTGCTATCGCTTGCGGGGGCCCGGGAGCTCTGGTCTGGATCTGGTTAGCCGCTCTTTTAGGTGC
CGTAGTCCAATACTCCGGATCTTTTTTAGGAATCAAATATCGAAGACTTCGTGGAGAGTCCGGGGAGTTTATCGGGGGGC
CCACAGCTTGCCTAGCTTACGGTATGGGAAGTAAATTTCTGGCCGGACTGTTCTGCATATTCACGATTATCACTGCTTTT
TCTGCAGGAAATTTCGCGCAAATTAATTGCATAGTTCCTCTGTGCGCGGAAAGCATTTCCTTAAAGTTTATTATAGGACT
CTTTCTTGCGTTAACAATTGTTCCCGTACTCATTGGTGGAAATCACCGCATTCTTAGATTTTCAGCTCGTGTAATTCCTT
TTATTGCTGGCTTCTACGCTCTCTCTTGTCTTATTATTCTCGCTCAACACAGCTCTATGATCCTTCCGGCATTGAAACTT
ATCGTCACCTCTGCTTTGGGAATCAAGGCTACGGTTGCCGGTTTGGGAGGCTATACAGTAACTCAGGTGATCTCAACAGG
AATGAGCCGAGCTATTATGGCAACAGATTGCGGTAGCGGTATGGTGTCAATTTTACAATCCGATTCTCGAAGTAAGAATC
CTGTGATTGATGGGTTGGTTACCCTCTTACCTCCTGTAGTTGTTGTTATGGTATGCTCGATTACTATGCTTGTTCTTATT
GTTTCGGGAGCTTATGTTTCAGGACAAGAAGGAACACTTATGGTGTTGCATGCTTTTAGATCAAGTCTGGGCTCCCTCGG
AGGATTGGTTGTTATCCTAGCAATGGCTTTATTTGGTTATACAACAGTTTTGACCTGGTTTGCTTGCGCAGAAAAAAGTT
TGGAATATATGATCCCAGGGAAACGGGCTAACTCTTTGTTGAAGATTCTTTTCGTTGCCGTTATCCCTCTTGGAGGCATA
GTAGACATGCGATGGATCTGGAGCCTTTCCGACACGGGGTTTGCGGGTATGGTGATTCTAAATTCTATAGCTTTGGTAGC
TTTGTTTAAGGATGTGTTATCAACAAATCGAGAAGTTGCCTTACTTAAGCTGAAAGAAGATGCTAAATCCAATGTTTTGC
AAAATCTAGATATCTAA

Upstream 100 bases:

>100_bases
TATAACGAAGCTTCCTTTCCCCTAATCCAGGAAAGGAAGCCACGAGCTAATTTTCCCGTGATCTTCCAAAGAACACTGTT
AGCAATAAAGAGAGAGACAA

Downstream 100 bases:

>100_bases
ACGGAGACGAGTTATGCAATTACTTTCACCAGCATTTGATTACGGAAAACCGATCCCTAAAAAATATACATGTCAAGGAA
TTAATATTTCTCCACCCTTA

Product: D-alanine/glycine permease

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MLHFLEQLNNFCISFCVFPMILFLGGLLTWKLRGLQFTGLKLGFDLMLKNKQESSSTVEGKVSRYEAVAGILAGNFGTGN
IAGMAIAIACGGPGALVWIWLAALLGAVVQYSGSFLGIKYRRLRGESGEFIGGPTACLAYGMGSKFLAGLFCIFTIITAF
SAGNFAQINCIVPLCAESISLKFIIGLFLALTIVPVLIGGNHRILRFSARVIPFIAGFYALSCLIILAQHSSMILPALKL
IVTSALGIKATVAGLGGYTVTQVISTGMSRAIMATDCGSGMVSILQSDSRSKNPVIDGLVTLLPPVVVVMVCSITMLVLI
VSGAYVSGQEGTLMVLHAFRSSLGSLGGLVVILAMALFGYTTVLTWFACAEKSLEYMIPGKRANSLLKILFVAVIPLGGI
VDMRWIWSLSDTGFAGMVILNSIALVALFKDVLSTNREVALLKLKEDAKSNVLQNLDI

Sequences:

>Translated_458_residues
MLHFLEQLNNFCISFCVFPMILFLGGLLTWKLRGLQFTGLKLGFDLMLKNKQESSSTVEGKVSRYEAVAGILAGNFGTGN
IAGMAIAIACGGPGALVWIWLAALLGAVVQYSGSFLGIKYRRLRGESGEFIGGPTACLAYGMGSKFLAGLFCIFTIITAF
SAGNFAQINCIVPLCAESISLKFIIGLFLALTIVPVLIGGNHRILRFSARVIPFIAGFYALSCLIILAQHSSMILPALKL
IVTSALGIKATVAGLGGYTVTQVISTGMSRAIMATDCGSGMVSILQSDSRSKNPVIDGLVTLLPPVVVVMVCSITMLVLI
VSGAYVSGQEGTLMVLHAFRSSLGSLGGLVVILAMALFGYTTVLTWFACAEKSLEYMIPGKRANSLLKILFVAVIPLGGI
VDMRWIWSLSDTGFAGMVILNSIALVALFKDVLSTNREVALLKLKEDAKSNVLQNLDI
>Mature_458_residues
MLHFLEQLNNFCISFCVFPMILFLGGLLTWKLRGLQFTGLKLGFDLMLKNKQESSSTVEGKVSRYEAVAGILAGNFGTGN
IAGMAIAIACGGPGALVWIWLAALLGAVVQYSGSFLGIKYRRLRGESGEFIGGPTACLAYGMGSKFLAGLFCIFTIITAF
SAGNFAQINCIVPLCAESISLKFIIGLFLALTIVPVLIGGNHRILRFSARVIPFIAGFYALSCLIILAQHSSMILPALKL
IVTSALGIKATVAGLGGYTVTQVISTGMSRAIMATDCGSGMVSILQSDSRSKNPVIDGLVTLLPPVVVVMVCSITMLVLI
VSGAYVSGQEGTLMVLHAFRSSLGSLGGLVVILAMALFGYTTVLTWFACAEKSLEYMIPGKRANSLLKILFVAVIPLGGI
VDMRWIWSLSDTGFAGMVILNSIALVALFKDVLSTNREVALLKLKEDAKSNVLQNLDI

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=391, Percent_Identity=26.5984654731458, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 48665; Mature: 48665

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHFLEQLNNFCISFCVFPMILFLGGLLTWKLRGLQFTGLKLGFDLMLKNKQESSSTVEG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCEECHHHHCCCCCCCCCHHHH
KVSRYEAVAGILAGNFGTGNIAGMAIAIACGGPGALVWIWLAALLGAVVQYSGSFLGIKY
HHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEH
RRLRGESGEFIGGPTACLAYGMGSKFLAGLFCIFTIITAFSAGNFAQINCIVPLCAESIS
HHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHH
LKFIIGLFLALTIVPVLIGGNHRILRFSARVIPFIAGFYALSCLIILAQHSSMILPALKL
HHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IVTSALGIKATVAGLGGYTVTQVISTGMSRAIMATDCGSGMVSILQSDSRSKNPVIDGLV
HHHHHCCHHHHHHCCCCHHHHHHHHHCHHHEEEEECCCCHHHHHHHCCCCCCCCHHHHHH
TLLPPVVVVMVCSITMLVLIVSGAYVSGQEGTLMVLHAFRSSLGSLGGLVVILAMALFGY
HHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
TTVLTWFACAEKSLEYMIPGKRANSLLKILFVAVIPLGGIVDMRWIWSLSDTGFAGMVIL
HHHHHHHHHHHHCHHEECCCHHHHHHHHHHHHHHHCCCCCEEHHEEEEECCCCCHHHHHH
NSIALVALFKDVLSTNREVALLKLKEDAKSNVLQNLDI
HHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCCC
>Mature Secondary Structure
MLHFLEQLNNFCISFCVFPMILFLGGLLTWKLRGLQFTGLKLGFDLMLKNKQESSSTVEG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCEECHHHHCCCCCCCCCHHHH
KVSRYEAVAGILAGNFGTGNIAGMAIAIACGGPGALVWIWLAALLGAVVQYSGSFLGIKY
HHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEH
RRLRGESGEFIGGPTACLAYGMGSKFLAGLFCIFTIITAFSAGNFAQINCIVPLCAESIS
HHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHH
LKFIIGLFLALTIVPVLIGGNHRILRFSARVIPFIAGFYALSCLIILAQHSSMILPALKL
HHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IVTSALGIKATVAGLGGYTVTQVISTGMSRAIMATDCGSGMVSILQSDSRSKNPVIDGLV
HHHHHCCHHHHHHCCCCHHHHHHHHHCHHHEEEEECCCCHHHHHHHCCCCCCCCHHHHHH
TLLPPVVVVMVCSITMLVLIVSGAYVSGQEGTLMVLHAFRSSLGSLGGLVVILAMALFGY
HHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
TTVLTWFACAEKSLEYMIPGKRANSLLKILFVAVIPLGGIVDMRWIWSLSDTGFAGMVIL
HHHHHHHHHHHHCHHEECCCHHHHHHHHHHHHHHHCCCCCEEHHEEEEECCCCCHHHHHH
NSIALVALFKDVLSTNREVALLKLKEDAKSNVLQNLDI
HHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]