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The map label for this gene is priA [H]

Identifier: 89257182

GI number: 89257182

Start: 1871080

End: 1873233

Strand: Direct

Name: priA [H]

Synonym: FTL_1943

Alternate gene names: 89257182

Gene position: 1871080-1873233 (Clockwise)

Preceding gene: 89257181

Following gene: 89257183

Centisome position: 98.69

GC content: 32.59

Gene sequence:

>2154_bases
ATGATTGTCAAGGTAGCTTTACCAGTACCACCTAGTTATGTACTTGACTATAGTGTTGATTGTCAAAATGTAGAGCTATT
CGAGCGTGTCTTAGTTCAGGTCGGAAAGCGTCAGCTAATAGGTTTTATTGTAGCAAAAGATGTAAAGATTGGTTATGAAG
CTGAAAAGCTTAAAACTATTATAAGAATTCTTGATAAACCGATTAGTCTAGCAATACAAAAGCTAATATCATGGCTATCA
CAATATTATTGCTGTGATATATACAGTGCAATACGTTTAGCACTACCAAATGACTTTCTCAAGCTACAAGAGGTTAAACC
ACAGCAGGATACATATGTATATATTGATAAAGTAAGGTTGCAACAACATAAACTAACAGCAAAGCAGCAAGATTTAATAA
GTGCTATTGCCACACAAGAGTTAATTAAATTAGAAGAATTAAAACAACTTGCTTCAAGCTATGTTATCAATAAGTTGTTC
GCTAATAATATCCTTAATAAAACTTATAAGCTAAAACAAAGCGAAAACGTTCTAAATACTGATAAGTCTAGAGTTCTTAA
CCAACAACAGCAATATGTGTTAGAGCAAATATTAGCTAATACAATTTTTAATGTATCGTTGCTATATGGTATTACTGGGA
GTGGTAAAACTGAAATTTATTTACAAGCAATAGCTGAGGTTTTAGCTAAATCTCAACAAGTGTTGATTTTAGTTCCGGAA
ATAAATCTTACTCCGCAAACAATCAAAAGATTTGAAAATAGATTTGTAGATAAAAATGTTGTTGCGCTACATTCTAAATT
AAGTGATAAAGCAAGGTTAACTAATTGGCTAAAGATAAAGCAAGGTAAAGCTGATATTGTAATTGCAACGCGTAGTGGTG
TTTTAGCAGACTTTTGTAATCTTGGAATGATTATAGTTGATGAAGAGCATGATGGCTCATTTAAGCAGCAAACTACTACT
ATTCGCTACAATGCTCGCGATGTTGCAATATTTAGAGCTAGACAACTTGATATACCAATAGTATTGGGAAGTGCAACACC
ATCACTACAAAGTTATTACAATTGTGTTAGTGGCAAATATCGCCTACTTAAATTAACTCAAAGAGCATTAAATTCGCATA
AAAATCAAATTGAACTTTTAGATCTGAAATCAGCTATTGTTGATAATGGTATTAGTAGTCAACTTTTTGCGCTTTTACAA
AAGAATATCCAAGCACATCAGCAGTCACTAGTTTTTATTAATAAATTAGGTTTTGCTAAAGCATTAGTATGCAAGAGTTG
TGGTGAGGTTGTTGAGTGTAAAAAATGTGATAAACCTTATACTTTACATACACAACCATATCAATACTTAGAGTGTCATT
TTTGTGGTTCGCGTAAGCCTCTGGTTACCGCATGCCCTAGCTGTGGAGCACAAGAGTTGTTTCCTTATGGTTGTGGGACT
GAAAAAATTCAAGCTCGAATAGAAGCAAAATTTCCTTACAATAGAGTTGTACGTTTTGATCGTGCAAATATTAAAACTAT
AACTGATTTACATGCTGTCAACCAGTTGATAAATGATAATCAGGTTGATGTTATAGTTGGTACACAAATGATTGCAAAAG
GACATCATTTTGAAAATATTACTCTAGTTGGTTTGATAAATATTGATGCCGGTTTATACAGTAGTGATTTTAACGCTATT
GAGAAAACAGCACAGATGATTGTACAGGTTGCAGGTAGAGCAGGTAGGGCAGATAAGCCTGGGACAATAGTATTACAAAC
TTATCAACCAGAAAATAAGCTACTTCAGTTATTGGTAAATAATGATTATTTAGAGTTTTTAGATTATCTTTTACAGCAAC
GAAAATATGCTGGTTATCCACCATATGCGTATCAAGCACAAATTATTGCAGAGTCAAAAAGACAAGGACAAGTTCTAGAG
TTGTTGAATGATGTATATTCAAGTATATCTGGCAGTGTAAATATTCAAGTGTCTAAACCTTTGCCAGCATTACATCTTAA
GCGTAATAATGTGTATAGATATAGCTTATTGCTAACAGCAAAAACGCGTAAAGAGATTAACTCAGTAGTTAAGCAAATTA
ATAATAAATTTGTTATAAATATACCAAGTACGGTAAAAATGTGTTTTGATATTGATCCTATTGAGTTAGCATAA

Upstream 100 bases:

>100_bases
AATTATATGGTCAAGTTCTACAAAATTTCATATTTTTTATTACTGCTGTTTATGGTTTTTACTATTGGTATATGAATACA
AAGGTAGCGGAAGCTAACAA

Downstream 100 bases:

>100_bases
ATGCAAAAGTGGAAGTTTTATTTTTCCGAGCTTCGATAAGATGCAAAATGTTAGATTGATTTTTATAGTTAATCCGAAAG
ATATTTGTAGAAAAAGATAT

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 717; Mature: 717

Protein sequence:

>717_residues
MIVKVALPVPPSYVLDYSVDCQNVELFERVLVQVGKRQLIGFIVAKDVKIGYEAEKLKTIIRILDKPISLAIQKLISWLS
QYYCCDIYSAIRLALPNDFLKLQEVKPQQDTYVYIDKVRLQQHKLTAKQQDLISAIATQELIKLEELKQLASSYVINKLF
ANNILNKTYKLKQSENVLNTDKSRVLNQQQQYVLEQILANTIFNVSLLYGITGSGKTEIYLQAIAEVLAKSQQVLILVPE
INLTPQTIKRFENRFVDKNVVALHSKLSDKARLTNWLKIKQGKADIVIATRSGVLADFCNLGMIIVDEEHDGSFKQQTTT
IRYNARDVAIFRARQLDIPIVLGSATPSLQSYYNCVSGKYRLLKLTQRALNSHKNQIELLDLKSAIVDNGISSQLFALLQ
KNIQAHQQSLVFINKLGFAKALVCKSCGEVVECKKCDKPYTLHTQPYQYLECHFCGSRKPLVTACPSCGAQELFPYGCGT
EKIQARIEAKFPYNRVVRFDRANIKTITDLHAVNQLINDNQVDVIVGTQMIAKGHHFENITLVGLINIDAGLYSSDFNAI
EKTAQMIVQVAGRAGRADKPGTIVLQTYQPENKLLQLLVNNDYLEFLDYLLQQRKYAGYPPYAYQAQIIAESKRQGQVLE
LLNDVYSSISGSVNIQVSKPLPALHLKRNNVYRYSLLLTAKTRKEINSVVKQINNKFVINIPSTVKMCFDIDPIELA

Sequences:

>Translated_717_residues
MIVKVALPVPPSYVLDYSVDCQNVELFERVLVQVGKRQLIGFIVAKDVKIGYEAEKLKTIIRILDKPISLAIQKLISWLS
QYYCCDIYSAIRLALPNDFLKLQEVKPQQDTYVYIDKVRLQQHKLTAKQQDLISAIATQELIKLEELKQLASSYVINKLF
ANNILNKTYKLKQSENVLNTDKSRVLNQQQQYVLEQILANTIFNVSLLYGITGSGKTEIYLQAIAEVLAKSQQVLILVPE
INLTPQTIKRFENRFVDKNVVALHSKLSDKARLTNWLKIKQGKADIVIATRSGVLADFCNLGMIIVDEEHDGSFKQQTTT
IRYNARDVAIFRARQLDIPIVLGSATPSLQSYYNCVSGKYRLLKLTQRALNSHKNQIELLDLKSAIVDNGISSQLFALLQ
KNIQAHQQSLVFINKLGFAKALVCKSCGEVVECKKCDKPYTLHTQPYQYLECHFCGSRKPLVTACPSCGAQELFPYGCGT
EKIQARIEAKFPYNRVVRFDRANIKTITDLHAVNQLINDNQVDVIVGTQMIAKGHHFENITLVGLINIDAGLYSSDFNAI
EKTAQMIVQVAGRAGRADKPGTIVLQTYQPENKLLQLLVNNDYLEFLDYLLQQRKYAGYPPYAYQAQIIAESKRQGQVLE
LLNDVYSSISGSVNIQVSKPLPALHLKRNNVYRYSLLLTAKTRKEINSVVKQINNKFVINIPSTVKMCFDIDPIELA
>Mature_717_residues
MIVKVALPVPPSYVLDYSVDCQNVELFERVLVQVGKRQLIGFIVAKDVKIGYEAEKLKTIIRILDKPISLAIQKLISWLS
QYYCCDIYSAIRLALPNDFLKLQEVKPQQDTYVYIDKVRLQQHKLTAKQQDLISAIATQELIKLEELKQLASSYVINKLF
ANNILNKTYKLKQSENVLNTDKSRVLNQQQQYVLEQILANTIFNVSLLYGITGSGKTEIYLQAIAEVLAKSQQVLILVPE
INLTPQTIKRFENRFVDKNVVALHSKLSDKARLTNWLKIKQGKADIVIATRSGVLADFCNLGMIIVDEEHDGSFKQQTTT
IRYNARDVAIFRARQLDIPIVLGSATPSLQSYYNCVSGKYRLLKLTQRALNSHKNQIELLDLKSAIVDNGISSQLFALLQ
KNIQAHQQSLVFINKLGFAKALVCKSCGEVVECKKCDKPYTLHTQPYQYLECHFCGSRKPLVTACPSCGAQELFPYGCGT
EKIQARIEAKFPYNRVVRFDRANIKTITDLHAVNQLINDNQVDVIVGTQMIAKGHHFENITLVGLINIDAGLYSSDFNAI
EKTAQMIVQVAGRAGRADKPGTIVLQTYQPENKLLQLLVNNDYLEFLDYLLQQRKYAGYPPYAYQAQIIAESKRQGQVLE
LLNDVYSSISGSVNIQVSKPLPALHLKRNNVYRYSLLLTAKTRKEINSVVKQINNKFVINIPSTVKMCFDIDPIELA

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=739, Percent_Identity=37.3477672530447, Blast_Score=464, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81044; Mature: 81044

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVKVALPVPPSYVLDYSVDCQNVELFERVLVQVGKRQLIGFIVAKDVKIGYEAEKLKTI
CEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCHHHHHHHHEEECCCCCCCHHHHHHH
IRILDKPISLAIQKLISWLSQYYCCDIYSAIRLALPNDFLKLQEVKPQQDTYVYIDKVRL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEEEHHH
QQHKLTAKQQDLISAIATQELIKLEELKQLASSYVINKLFANNILNKTYKLKQSENVLNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCC
DKSRVLNQQQQYVLEQILANTIFNVSLLYGITGSGKTEIYLQAIAEVLAKSQQVLILVPE
CHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECC
INLTPQTIKRFENRFVDKNVVALHSKLSDKARLTNWLKIKQGKADIVIATRSGVLADFCN
CCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHEEEECCCCCEEEEECCCHHHHHHC
LGMIIVDEEHDGSFKQQTTTIRYNARDVAIFRARQLDIPIVLGSATPSLQSYYNCVSGKY
CCEEEEECCCCCCCHHCEEEEEECCCEEEEEEEEECCCEEEECCCCCHHHHHHHHHCCCH
RLLKLTQRALNSHKNQIELLDLKSAIVDNGISSQLFALLQKNIQAHQQSLVFINKLGFAK
HHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVCKSCGEVVECKKCDKPYTLHTQPYQYLECHFCGSRKPLVTACPSCGAQELFPYGCGT
HHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEEECCCCCCCEEECCCCCCHHCCCCCCCC
EKIQARIEAKFPYNRVVRFDRANIKTITDLHAVNQLINDNQVDVIVGTQMIAKGHHFENI
HHHHEEEEECCCHHHHEEECCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCCCE
TLVGLINIDAGLYSSDFNAIEKTAQMIVQVAGRAGRADKPGTIVLQTYQPENKLLQLLVN
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHHHHHC
NDYLEFLDYLLQQRKYAGYPPYAYQAQIIAESKRQGQVLELLNDVYSSISGSVNIQVSKP
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCC
LPALHLKRNNVYRYSLLLTAKTRKEINSVVKQINNKFVINIPSTVKMCFDIDPIELA
CCEEEEECCCEEEEEEEEEEHHHHHHHHHHHHCCCCEEEECCHHEEHEECCCCCCCC
>Mature Secondary Structure
MIVKVALPVPPSYVLDYSVDCQNVELFERVLVQVGKRQLIGFIVAKDVKIGYEAEKLKTI
CEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCHHHHHHHHEEECCCCCCCHHHHHHH
IRILDKPISLAIQKLISWLSQYYCCDIYSAIRLALPNDFLKLQEVKPQQDTYVYIDKVRL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEEEHHH
QQHKLTAKQQDLISAIATQELIKLEELKQLASSYVINKLFANNILNKTYKLKQSENVLNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCC
DKSRVLNQQQQYVLEQILANTIFNVSLLYGITGSGKTEIYLQAIAEVLAKSQQVLILVPE
CHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECC
INLTPQTIKRFENRFVDKNVVALHSKLSDKARLTNWLKIKQGKADIVIATRSGVLADFCN
CCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHEEEECCCCCEEEEECCCHHHHHHC
LGMIIVDEEHDGSFKQQTTTIRYNARDVAIFRARQLDIPIVLGSATPSLQSYYNCVSGKY
CCEEEEECCCCCCCHHCEEEEEECCCEEEEEEEEECCCEEEECCCCCHHHHHHHHHCCCH
RLLKLTQRALNSHKNQIELLDLKSAIVDNGISSQLFALLQKNIQAHQQSLVFINKLGFAK
HHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVCKSCGEVVECKKCDKPYTLHTQPYQYLECHFCGSRKPLVTACPSCGAQELFPYGCGT
HHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEEECCCCCCCEEECCCCCCHHCCCCCCCC
EKIQARIEAKFPYNRVVRFDRANIKTITDLHAVNQLINDNQVDVIVGTQMIAKGHHFENI
HHHHEEEEECCCHHHHEEECCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCCCE
TLVGLINIDAGLYSSDFNAIEKTAQMIVQVAGRAGRADKPGTIVLQTYQPENKLLQLLVN
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHHHHHC
NDYLEFLDYLLQQRKYAGYPPYAYQAQIIAESKRQGQVLELLNDVYSSISGSVNIQVSKP
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCC
LPALHLKRNNVYRYSLLLTAKTRKEINSVVKQINNKFVINIPSTVKMCFDIDPIELA
CCEEEEECCCEEEEEEEEEEHHHHHHHHHHHHCCCCEEEECCHHEEHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]