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The map label for this gene is prfB [H]

Identifier: 89257152

GI number: 89257152

Start: 1836113

End: 1837090

Strand: Reverse

Name: prfB [H]

Synonym: FTL_1904

Alternate gene names: 89257152

Gene position: 1837090-1836113 (Counterclockwise)

Preceding gene: 89257154

Following gene: 89257151

Centisome position: 96.89

GC content: 38.75

Gene sequence:

>978_bases
ATGGAGCTAGAAGATGGTTCTATTTGGGATAATCCAGAGTATGCACAAAACTTGGGTAAACAAAAAGTAGAACTTGAAAA
CGTTGTACATAATTGTGAGTATATCTCAGAAACTCTAGAAACTTTAAGTGAACTGCTTGAGCTTGGTGAAGAAGATGAAT
CTTTAATGCAAGAAATTGCTAAAGATACACAAAATGTTGCTAGTGAAATTGAGAAGCTTGAGTTTCGTCGCATGTTTTCA
GGTAAAATGGATGCTAATAAAGCCTTTTTGGATATTCAGTCAGGTTCTGGAGGTACAGAGGCGCAAGATTGGGCAGAGAT
GCTGATGCGGATGTATATGCGTTGGGCAGATAGTCACGGCTTCAAGGTAACCGTTGATGATGTATCAGATGGTGATGTTG
CGGGTATAAAAGGCTGCACCTTAAAAATAGAGGGAGAGTATGCTTATGGTTGGTTGCGTACCGAAACTGGTATTCATAGG
TTAGTACGCAAATCACCTTTTGACTCAAACAGTAAACGTCATACATCTTTTGCATCGGTGTTTATCTCACCTGAGGTTGA
TGATGATATTGATATTGAGATAAATCCTGCTGATTTACGAGTCGATACTTATCGTGCATCAGGTGCTGGTGGTCAGCATG
TCAACAAAACAGACTCTGCTGTGAGAATAACGCATATTCCGACAAATATTGTGGTACAAAGTCAAAGTGATAGATCTCAG
CATAAAAATAGAGATAATGCCATGAAACAGCTTAAGTCAAAGCTATATGAAATGGAGTTACAAAAGCGTAATGCTGAAAA
GAATGCACTCGAAGACTCAAAGGCAGATATTGGTTGGGGTAGTCAGATTCGTTCGTACGTGTTGGATCAGTCACGAATTA
AGGATTTAAGAACAGGAGTTGAGAATACTAATACACAAGCGGTATTAGATGGTGATTTGGATAAGTTTATTGAGGCTAGC
TTAAAAAGTGGGTTATAA

Upstream 100 bases:

>100_bases
AATTATAAGATTGTATTAAAAGATCTAACGGCCCGTATTCAATCTTTACGGGAATATCTTTGACTATGATACTAAGAAAG
AAAAGCTAACAGAAGTCTTA

Downstream 100 bases:

>100_bases
TAAATAGATTTTATAAAAAGTATGCAAAACTGCCTAGCACTACGCTGTAGTTTAAAACAAATGAAAAATAAAAAACTCGC
CAATTTGAAAATTGATTCAG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MELEDGSIWDNPEYAQNLGKQKVELENVVHNCEYISETLETLSELLELGEEDESLMQEIAKDTQNVASEIEKLEFRRMFS
GKMDANKAFLDIQSGSGGTEAQDWAEMLMRMYMRWADSHGFKVTVDDVSDGDVAGIKGCTLKIEGEYAYGWLRTETGIHR
LVRKSPFDSNSKRHTSFASVFISPEVDDDIDIEINPADLRVDTYRASGAGGQHVNKTDSAVRITHIPTNIVVQSQSDRSQ
HKNRDNAMKQLKSKLYEMELQKRNAEKNALEDSKADIGWGSQIRSYVLDQSRIKDLRTGVENTNTQAVLDGDLDKFIEAS
LKSGL

Sequences:

>Translated_325_residues
MELEDGSIWDNPEYAQNLGKQKVELENVVHNCEYISETLETLSELLELGEEDESLMQEIAKDTQNVASEIEKLEFRRMFS
GKMDANKAFLDIQSGSGGTEAQDWAEMLMRMYMRWADSHGFKVTVDDVSDGDVAGIKGCTLKIEGEYAYGWLRTETGIHR
LVRKSPFDSNSKRHTSFASVFISPEVDDDIDIEINPADLRVDTYRASGAGGQHVNKTDSAVRITHIPTNIVVQSQSDRSQ
HKNRDNAMKQLKSKLYEMELQKRNAEKNALEDSKADIGWGSQIRSYVLDQSRIKDLRTGVENTNTQAVLDGDLDKFIEAS
LKSGL
>Mature_325_residues
MELEDGSIWDNPEYAQNLGKQKVELENVVHNCEYISETLETLSELLELGEEDESLMQEIAKDTQNVASEIEKLEFRRMFS
GKMDANKAFLDIQSGSGGTEAQDWAEMLMRMYMRWADSHGFKVTVDDVSDGDVAGIKGCTLKIEGEYAYGWLRTETGIHR
LVRKSPFDSNSKRHTSFASVFISPEVDDDIDIEINPADLRVDTYRASGAGGQHVNKTDSAVRITHIPTNIVVQSQSDRSQ
HKNRDNAMKQLKSKLYEMELQKRNAEKNALEDSKADIGWGSQIRSYVLDQSRIKDLRTGVENTNTQAVLDGDLDKFIEAS
LKSGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI34577120, Length=334, Percent_Identity=32.3353293413174, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI166795303, Length=279, Percent_Identity=34.0501792114695, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI166795305, Length=186, Percent_Identity=34.9462365591398, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI2367172, Length=326, Percent_Identity=67.1779141104295, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1787462, Length=240, Percent_Identity=40.8333333333333, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17542784, Length=256, Percent_Identity=30.078125, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=297, Percent_Identity=34.6801346801347, Blast_Score=148, Evalue=9e-37,
Organism=Drosophila melanogaster, GI19921226, Length=251, Percent_Identity=36.2549800796813, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 36609; Mature: 36609

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELEDGSIWDNPEYAQNLGKQKVELENVVHNCEYISETLETLSELLELGEEDESLMQEIA
CCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KDTQNVASEIEKLEFRRMFSGKMDANKAFLDIQSGSGGTEAQDWAEMLMRMYMRWADSHG
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCC
FKVTVDDVSDGDVAGIKGCTLKIEGEYAYGWLRTETGIHRLVRKSPFDSNSKRHTSFASV
CEEEECCCCCCCCCCCCCEEEEEECCEEEEEEEHHHHHHHHHHCCCCCCCCCCCCEEEEE
FISPEVDDDIDIEINPADLRVDTYRASGAGGQHVNKTDSAVRITHIPTNIVVQSQSDRSQ
EECCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEECCCHHHH
HKNRDNAMKQLKSKLYEMELQKRNAEKNALEDSKADIGWGSQIRSYVLDQSRIKDLRTGV
HCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
ENTNTQAVLDGDLDKFIEASLKSGL
CCCCCCEEECCCHHHHHHHHHHCCC
>Mature Secondary Structure
MELEDGSIWDNPEYAQNLGKQKVELENVVHNCEYISETLETLSELLELGEEDESLMQEIA
CCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KDTQNVASEIEKLEFRRMFSGKMDANKAFLDIQSGSGGTEAQDWAEMLMRMYMRWADSHG
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCC
FKVTVDDVSDGDVAGIKGCTLKIEGEYAYGWLRTETGIHRLVRKSPFDSNSKRHTSFASV
CEEEECCCCCCCCCCCCCEEEEEECCEEEEEEEHHHHHHHHHHCCCCCCCCCCCCEEEEE
FISPEVDDDIDIEINPADLRVDTYRASGAGGQHVNKTDSAVRITHIPTNIVVQSQSDRSQ
EECCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEECCCHHHH
HKNRDNAMKQLKSKLYEMELQKRNAEKNALEDSKADIGWGSQIRSYVLDQSRIKDLRTGV
HCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
ENTNTQAVLDGDLDKFIEASLKSGL
CCCCCCEEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA