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The map label for this gene is leuC [H]

Identifier: 89257140

GI number: 89257140

Start: 1821265

End: 1822668

Strand: Reverse

Name: leuC [H]

Synonym: FTL_1889

Alternate gene names: 89257140

Gene position: 1822668-1821265 (Counterclockwise)

Preceding gene: 89257142

Following gene: 89257139

Centisome position: 96.13

GC content: 36.54

Gene sequence:

>1404_bases
ATGGTAAGAAATATTATCGATAAAATCTGGGATGCTCATGTTGTAAAACAAATACCTAATTTTCCAGATATTTTATATAT
AGATAGAATACTAATGCATGAGGTAACTTCTGCACAAGCTTTCGATAGAGAACTAAATATTCCAATAAATAATCCAAAAT
CGATCATTGCAACTGTTGATCATAGTATCTCGACATCACCTATCAATCGTCTGGAAATGAAAGATAAAGTTGCTCAAGCT
CAAGTAGAAAAATTACGTAGTAATGTTAAAGAATTTGGTATTGATTTTTATGACTTTGAAAGCCAGCATCAAGGAATTGT
CCATGTTATAGGTCCTGAGCTTGGCTTCACACTACCTGGTACAACTCTTGTATATGGCGACTCGCATACATCTACGCATG
GTGCATTTGGAGCTTTAGCATTCGGTGTTGGAACATCTGAAGTTGGACATGTACTTGCTACTAACTGTATTTTGCAATAC
AGACCAAAGACAATGAAAGTAGAGTTTGTTGGGAAGCCTTCAAAGTCAGCTACGGCTAAAGATATTATCATGAAGCTAAT
AGCTAATATTGGTATTGGTGGTACTGGTGGTTATGTCATCGAATATGTTGGTCAAGCAATCAAAGATATGACTATGGAAG
AGAGTATGACGCTATGTAATATGTCGATAGAATGTGGTGCAAGAGCTGGCTTGGTTTCTCCAGATGAGAAAACTTTTAGT
TACCTAAAAGGTAAAAAATATGCGCCTCAAGGTAATGATTTTGACAAGTCTGTAGAATATTGGAATAGCTTCATAAGTGA
TGAAAATGCTCACTATGACAAAACTATCAAAGTCGATATTGAAGGACTTGAGCCAATGGTTACATGGGGAATAAACCCTC
AACATGCTATCAGTATCTCAGCTAAGATTCCCAGCCTAAAAGATATACCTACTCATCAACACAAGCTAGCTCAACAAGCT
TATGATTATACTAAATTTAATGCTGATGAAAATATCCTAGGTAAAGAAATTCAATGGGCATTTGTTGGTAGCTGTACTAA
CGGTCGTATTGAAGATATGCGCGCTGTGGCAGATGTTCTAAAAGGTAGAAAAATCGCTAAAAATGTAACTATGTATATAG
TTCCTGGGTCAGAGCAAGTGCGAAATATCGCTATAGCTGAGGGGTTAGATAAGATCTTTGCTGATGCTGGAGCTGAATTT
AGAATGCCTGGTTGCTCAATGTGCTTAGCAATGAATGATGATAAAGTTCCAGAAGGTCAAAGATGTATTAGTACTTCAAA
TAGAAACTTCATCGGTCGTCAAGGTAAAGGAAGTATAACCCATCTTGCTTCACCACAAACAGTTGCTGCTAGTGCTGTTA
TGGGTAAGATTTGTAGTGTTGATAAATTAGATAAGGAGTTATAA

Upstream 100 bases:

>100_bases
GAAGGTGTTGCTATAGATCAAGATATCGAAATATCTTCACTTAAAGCATTAATAGCAGCAACTAATAAACTTTATATATA
AACAAATTATAGGAAGTAAT

Downstream 100 bases:

>100_bases
CAATGCAAGCTTTTAAAAAACTAACATCTAGCGCAATTCCTTTATGGCTAAGTGATATTGATACAGATATGATAATCCCT
GCTAACTTCCTAACTCAAAC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MVRNIIDKIWDAHVVKQIPNFPDILYIDRILMHEVTSAQAFDRELNIPINNPKSIIATVDHSISTSPINRLEMKDKVAQA
QVEKLRSNVKEFGIDFYDFESQHQGIVHVIGPELGFTLPGTTLVYGDSHTSTHGAFGALAFGVGTSEVGHVLATNCILQY
RPKTMKVEFVGKPSKSATAKDIIMKLIANIGIGGTGGYVIEYVGQAIKDMTMEESMTLCNMSIECGARAGLVSPDEKTFS
YLKGKKYAPQGNDFDKSVEYWNSFISDENAHYDKTIKVDIEGLEPMVTWGINPQHAISISAKIPSLKDIPTHQHKLAQQA
YDYTKFNADENILGKEIQWAFVGSCTNGRIEDMRAVADVLKGRKIAKNVTMYIVPGSEQVRNIAIAEGLDKIFADAGAEF
RMPGCSMCLAMNDDKVPEGQRCISTSNRNFIGRQGKGSITHLASPQTVAASAVMGKICSVDKLDKEL

Sequences:

>Translated_467_residues
MVRNIIDKIWDAHVVKQIPNFPDILYIDRILMHEVTSAQAFDRELNIPINNPKSIIATVDHSISTSPINRLEMKDKVAQA
QVEKLRSNVKEFGIDFYDFESQHQGIVHVIGPELGFTLPGTTLVYGDSHTSTHGAFGALAFGVGTSEVGHVLATNCILQY
RPKTMKVEFVGKPSKSATAKDIIMKLIANIGIGGTGGYVIEYVGQAIKDMTMEESMTLCNMSIECGARAGLVSPDEKTFS
YLKGKKYAPQGNDFDKSVEYWNSFISDENAHYDKTIKVDIEGLEPMVTWGINPQHAISISAKIPSLKDIPTHQHKLAQQA
YDYTKFNADENILGKEIQWAFVGSCTNGRIEDMRAVADVLKGRKIAKNVTMYIVPGSEQVRNIAIAEGLDKIFADAGAEF
RMPGCSMCLAMNDDKVPEGQRCISTSNRNFIGRQGKGSITHLASPQTVAASAVMGKICSVDKLDKEL
>Mature_467_residues
MVRNIIDKIWDAHVVKQIPNFPDILYIDRILMHEVTSAQAFDRELNIPINNPKSIIATVDHSISTSPINRLEMKDKVAQA
QVEKLRSNVKEFGIDFYDFESQHQGIVHVIGPELGFTLPGTTLVYGDSHTSTHGAFGALAFGVGTSEVGHVLATNCILQY
RPKTMKVEFVGKPSKSATAKDIIMKLIANIGIGGTGGYVIEYVGQAIKDMTMEESMTLCNMSIECGARAGLVSPDEKTFS
YLKGKKYAPQGNDFDKSVEYWNSFISDENAHYDKTIKVDIEGLEPMVTWGINPQHAISISAKIPSLKDIPTHQHKLAQQA
YDYTKFNADENILGKEIQWAFVGSCTNGRIEDMRAVADVLKGRKIAKNVTMYIVPGSEQVRNIAIAEGLDKIFADAGAEF
RMPGCSMCLAMNDDKVPEGQRCISTSNRNFIGRQGKGSITHLASPQTVAASAVMGKICSVDKLDKEL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=450, Percent_Identity=26, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI8659555, Length=460, Percent_Identity=23.9130434782609, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI41352693, Length=367, Percent_Identity=22.0708446866485, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1786259, Length=468, Percent_Identity=50.6410256410256, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI87081781, Length=381, Percent_Identity=23.6220472440945, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI2367097, Length=345, Percent_Identity=23.4782608695652, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787531, Length=364, Percent_Identity=23.0769230769231, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=448, Percent_Identity=27.4553571428571, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI32564738, Length=377, Percent_Identity=29.4429708222812, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI25149342, Length=306, Percent_Identity=28.7581699346405, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17568399, Length=445, Percent_Identity=23.1460674157303, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321429, Length=453, Percent_Identity=47.682119205298, Blast_Score=422, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6322261, Length=445, Percent_Identity=27.8651685393258, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6323335, Length=406, Percent_Identity=27.3399014778325, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6320440, Length=427, Percent_Identity=27.8688524590164, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI281365315, Length=447, Percent_Identity=27.7404921700224, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17864292, Length=447, Percent_Identity=27.7404921700224, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28571643, Length=405, Percent_Identity=27.4074074074074, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161076999, Length=382, Percent_Identity=28.7958115183246, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI17137564, Length=461, Percent_Identity=22.3427331887202, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24645686, Length=383, Percent_Identity=22.976501305483, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 51340; Mature: 51340

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00450 ACONITASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRNIIDKIWDAHVVKQIPNFPDILYIDRILMHEVTSAQAFDRELNIPINNPKSIIATVD
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEC
HSISTSPINRLEMKDKVAQAQVEKLRSNVKEFGIDFYDFESQHQGIVHVIGPELGFTLPG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCCCCCCC
TTLVYGDSHTSTHGAFGALAFGVGTSEVGHVLATNCILQYRPKTMKVEFVGKPSKSATAK
CEEEECCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHEEECCCEEEEEEECCCCCCCHHH
DIIMKLIANIGIGGTGGYVIEYVGQAIKDMTMEESMTLCNMSIECGARAGLVSPDEKTFS
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
YLKGKKYAPQGNDFDKSVEYWNSFISDENAHYDKTIKVDIEGLEPMVTWGINPQHAISIS
HHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCEEEEE
AKIPSLKDIPTHQHKLAQQAYDYTKFNADENILGKEIQWAFVGSCTNGRIEDMRAVADVL
ECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHH
KGRKIAKNVTMYIVPGSEQVRNIAIAEGLDKIFADAGAEFRMPGCSMCLAMNDDKVPEGQ
CCCHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCC
RCISTSNRNFIGRQGKGSITHLASPQTVAASAVMGKICSVDKLDKEL
HHHCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCHHHHHCCC
>Mature Secondary Structure
MVRNIIDKIWDAHVVKQIPNFPDILYIDRILMHEVTSAQAFDRELNIPINNPKSIIATVD
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEC
HSISTSPINRLEMKDKVAQAQVEKLRSNVKEFGIDFYDFESQHQGIVHVIGPELGFTLPG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCCCCCCC
TTLVYGDSHTSTHGAFGALAFGVGTSEVGHVLATNCILQYRPKTMKVEFVGKPSKSATAK
CEEEECCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHEEECCCEEEEEEECCCCCCCHHH
DIIMKLIANIGIGGTGGYVIEYVGQAIKDMTMEESMTLCNMSIECGARAGLVSPDEKTFS
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
YLKGKKYAPQGNDFDKSVEYWNSFISDENAHYDKTIKVDIEGLEPMVTWGINPQHAISIS
HHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCEEEEE
AKIPSLKDIPTHQHKLAQQAYDYTKFNADENILGKEIQWAFVGSCTNGRIEDMRAVADVL
ECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHH
KGRKIAKNVTMYIVPGSEQVRNIAIAEGLDKIFADAGAEFRMPGCSMCLAMNDDKVPEGQ
CCCHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCC
RCISTSNRNFIGRQGKGSITHLASPQTVAASAVMGKICSVDKLDKEL
HHHCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA