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The map label for this gene is tolC [H]

Identifier: 89257120

GI number: 89257120

Start: 1795635

End: 1797164

Strand: Reverse

Name: tolC [H]

Synonym: FTL_1865

Alternate gene names: 89257120

Gene position: 1797164-1795635 (Counterclockwise)

Preceding gene: 89257121

Following gene: 89257119

Centisome position: 94.79

GC content: 35.95

Gene sequence:

>1530_bases
ATGAAGAAGTTAACATTATATCTTTTAGGTTTTTTGGGGTTTTGTCTAGCTTTAGCTAATGAAGATACGCCTGTTCCTGA
ATATAAGTATGCAGGAAGACCTATTGGTACTGATGTTGCTGAAAATCCATATAGTGTCGAAAAACAATATGCCAAAGCAG
ATCAAATTAAGGTAGACAATAAATTCTATAATCTTAAAAAAGCTGATGAAGTCGCGATGGTTGGCGATACTGAGGACAAG
TATTATAGTTTAATTGATATCTATAAGTTATCTGCTGAACATAATGCCGACTACCAAGCAGCAAGGTCTACTTTTGCGGC
AAATGTTGAAACTGTGCCGACTGCTTTAGGTGCATTATTACCACAGATTGATTTTAACTATAATCTAAGAAGAGATTTGT
ACAATCAGTTTGGTGGTAGAGTTAATGACACTTCAAATGTAGTAAATTTTAGTGGTTCTCAGGTTCTTTTTGATTGGAGT
AAATGGAAGACATATACTCAAGCAACCTATTTGCAAAAATCGTATGCGATGATTTATGCTAAAGCTGAACAAACACTTAT
AACTGATACTATTACTGCATATTTTGAGCTGCTAAGAGCAGAACAGGCGTTACAGTTCCAATTTGCAAATGAGGCTTGGA
ACAAAAAGTTATATCTGACTCAGAAATATCAGTATAATTCGGGCATGGTTTCATATGCTGATTTTAAAACTACAGATGCA
CAATATCGTCAAGCAATCGCTGATAGGGTTAATGCACAAAGAAACTTCATTAATGCCAAAGCTGTGATGGCTAAACTTAT
CGGTAAGCGTATAAGCTCAATTTTATATATATCAAAAGATACTGAATTTGGTAATCCTGTTCCAGATGATATTAACTATT
GGTTGAATACAGCTGAGAAATATAATCTTGATATAGCGCAGAAAAAGTTTGAATATGAAGCAGCACAAGAAGGTGTTGGT
ATTCAATGGGGTAAATTTTTTCCTCAAGCTAATTTAACTGGCGGTATAATGATGTCACGTAACGCTCTTTCGGGTTCACC
AGCACAAATAGCCGAAATTCCAACCAAATATGACGTTGCTAATATCGGTGGTCAAGTTAGCTGGAATTTATTAAGAGGTG
GTTCTGACTATGCGCAACTCAAGCAAGCTAGTTATGATAATCAAGCGGCGAACTATGCCTTGCTACAAACCAAGAGAGAA
GTCTACGCTGGTACAGTAGAGGCATATCAAACAGTTGTTCTTGATGCAGTTAGAATTGAGGCGTTTAGAAAGTCTGTGTA
TTCAGGTATAGCTTCTGTAAAAGCGATATTAGAAGGTTTTGAAGCAGGTACACAGACAATCGTTGACTTACTTAACCGTC
AGGCAATCCTTGTCCAAGCACAATTATCATTTGCAGATGCTATATTCAACTATGTTGAAGATTATGTAAGTTTAAAGCAA
TTACAGGGGAGTTTAACCTACAAAGACATTGAGTACATCAATACTATTTTAGGAACTACAGATATAATATCGCAGATTGC
AACGGAGTAA

Upstream 100 bases:

>100_bases
GAACAGCTATATGTGATAAAGCATACTTAATAACAAAAACTCCAAAGCAAACATACGAAAAGAGTTTTCTCTTTAATACA
TACAATAAGTAAAGAGTGCT

Downstream 100 bases:

>100_bases
TTAGTTTGATGCCACAAGTCTCAGCTTTTATTTTAGATTTTGAGAAATATAAAGCTGAGGACCTTAGAGGATATCTTTGT
TGCAGAAATATTGATTCAGC

Product: outer membrane protein tolC precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MKKLTLYLLGFLGFCLALANEDTPVPEYKYAGRPIGTDVAENPYSVEKQYAKADQIKVDNKFYNLKKADEVAMVGDTEDK
YYSLIDIYKLSAEHNADYQAARSTFAANVETVPTALGALLPQIDFNYNLRRDLYNQFGGRVNDTSNVVNFSGSQVLFDWS
KWKTYTQATYLQKSYAMIYAKAEQTLITDTITAYFELLRAEQALQFQFANEAWNKKLYLTQKYQYNSGMVSYADFKTTDA
QYRQAIADRVNAQRNFINAKAVMAKLIGKRISSILYISKDTEFGNPVPDDINYWLNTAEKYNLDIAQKKFEYEAAQEGVG
IQWGKFFPQANLTGGIMMSRNALSGSPAQIAEIPTKYDVANIGGQVSWNLLRGGSDYAQLKQASYDNQAANYALLQTKRE
VYAGTVEAYQTVVLDAVRIEAFRKSVYSGIASVKAILEGFEAGTQTIVDLLNRQAILVQAQLSFADAIFNYVEDYVSLKQ
LQGSLTYKDIEYINTILGTTDIISQIATE

Sequences:

>Translated_509_residues
MKKLTLYLLGFLGFCLALANEDTPVPEYKYAGRPIGTDVAENPYSVEKQYAKADQIKVDNKFYNLKKADEVAMVGDTEDK
YYSLIDIYKLSAEHNADYQAARSTFAANVETVPTALGALLPQIDFNYNLRRDLYNQFGGRVNDTSNVVNFSGSQVLFDWS
KWKTYTQATYLQKSYAMIYAKAEQTLITDTITAYFELLRAEQALQFQFANEAWNKKLYLTQKYQYNSGMVSYADFKTTDA
QYRQAIADRVNAQRNFINAKAVMAKLIGKRISSILYISKDTEFGNPVPDDINYWLNTAEKYNLDIAQKKFEYEAAQEGVG
IQWGKFFPQANLTGGIMMSRNALSGSPAQIAEIPTKYDVANIGGQVSWNLLRGGSDYAQLKQASYDNQAANYALLQTKRE
VYAGTVEAYQTVVLDAVRIEAFRKSVYSGIASVKAILEGFEAGTQTIVDLLNRQAILVQAQLSFADAIFNYVEDYVSLKQ
LQGSLTYKDIEYINTILGTTDIISQIATE
>Mature_509_residues
MKKLTLYLLGFLGFCLALANEDTPVPEYKYAGRPIGTDVAENPYSVEKQYAKADQIKVDNKFYNLKKADEVAMVGDTEDK
YYSLIDIYKLSAEHNADYQAARSTFAANVETVPTALGALLPQIDFNYNLRRDLYNQFGGRVNDTSNVVNFSGSQVLFDWS
KWKTYTQATYLQKSYAMIYAKAEQTLITDTITAYFELLRAEQALQFQFANEAWNKKLYLTQKYQYNSGMVSYADFKTTDA
QYRQAIADRVNAQRNFINAKAVMAKLIGKRISSILYISKDTEFGNPVPDDINYWLNTAEKYNLDIAQKKFEYEAAQEGVG
IQWGKFFPQANLTGGIMMSRNALSGSPAQIAEIPTKYDVANIGGQVSWNLLRGGSDYAQLKQASYDNQAANYALLQTKRE
VYAGTVEAYQTVVLDAVRIEAFRKSVYSGIASVKAILEGFEAGTQTIVDLLNRQAILVQAQLSFADAIFNYVEDYVSLKQ
LQGSLTYKDIEYINTILGTTDIISQIATE

Specific function: Required for proper expression of outer membrane protein genes such as ompF, nmpC, protein 2, hemolysin, colicin V, or colicin E1. May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=429, Percent_Identity=28.2051282051282, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 57244; Mature: 57244

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTLYLLGFLGFCLALANEDTPVPEYKYAGRPIGTDVAENPYSVEKQYAKADQIKVDN
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCEEECC
KFYNLKKADEVAMVGDTEDKYYSLIDIYKLSAEHNADYQAARSTFAANVETVPTALGALL
CEECCCCCCCEEEECCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHH
PQIDFNYNLRRDLYNQFGGRVNDTSNVVNFSGSQVLFDWSKWKTYTQATYLQKSYAMIYA
CCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHHEEEE
KAEQTLITDTITAYFELLRAEQALQFQFANEAWNKKLYLTQKYQYNSGMVSYADFKTTDA
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEECCCCCCHH
QYRQAIADRVNAQRNFINAKAVMAKLIGKRISSILYISKDTEFGNPVPDDINYWLNTAEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHCCCHH
YNLDIAQKKFEYEAAQEGVGIQWGKFFPQANLTGGIMMSRNALSGSPAQIAEIPTKYDVA
CCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCEEEECCCCCCCCHHHHHCCCCCCHH
NIGGQVSWNLLRGGSDYAQLKQASYDNQAANYALLQTKREVYAGTVEAYQTVVLDAVRIE
HCCCCEEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFRKSVYSGIASVKAILEGFEAGTQTIVDLLNRQAILVQAQLSFADAIFNYVEDYVSLKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
LQGSLTYKDIEYINTILGTTDIISQIATE
HHCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLTLYLLGFLGFCLALANEDTPVPEYKYAGRPIGTDVAENPYSVEKQYAKADQIKVDN
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCEEECC
KFYNLKKADEVAMVGDTEDKYYSLIDIYKLSAEHNADYQAARSTFAANVETVPTALGALL
CEECCCCCCCEEEECCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHH
PQIDFNYNLRRDLYNQFGGRVNDTSNVVNFSGSQVLFDWSKWKTYTQATYLQKSYAMIYA
CCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHHEEEE
KAEQTLITDTITAYFELLRAEQALQFQFANEAWNKKLYLTQKYQYNSGMVSYADFKTTDA
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEECCCCCCHH
QYRQAIADRVNAQRNFINAKAVMAKLIGKRISSILYISKDTEFGNPVPDDINYWLNTAEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHCCCHH
YNLDIAQKKFEYEAAQEGVGIQWGKFFPQANLTGGIMMSRNALSGSPAQIAEIPTKYDVA
CCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCEEEECCCCCCCCHHHHHCCCCCCHH
NIGGQVSWNLLRGGSDYAQLKQASYDNQAANYALLQTKREVYAGTVEAYQTVVLDAVRIE
HCCCCEEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFRKSVYSGIASVKAILEGFEAGTQTIVDLLNRQAILVQAQLSFADAIFNYVEDYVSLKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
LQGSLTYKDIEYINTILGTTDIISQIATE
HHCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6312426; 2216730; 9278503; 6303857; 9298646; 9629924; 10879525 [H]