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The map label for this gene is purB [H]

Identifier: 89257110

GI number: 89257110

Start: 1781314

End: 1782612

Strand: Reverse

Name: purB [H]

Synonym: FTL_1850

Alternate gene names: 89257110

Gene position: 1782612-1781314 (Counterclockwise)

Preceding gene: 89257111

Following gene: 89257109

Centisome position: 94.02

GC content: 35.49

Gene sequence:

>1299_bases
ATGATAAAAAGATATGACATAGCAGAAATCTCAAAAATTTGGGCAGATGAGAATAAATATGCAAAAATGTTAGAGGTTGA
GCTTGCGATTTTAGAGGCACTTGAAGATAGAATGGTACCTAAAGGTACAGCTGCGGAAATTCGTGCTAGAGCACAAATTA
GACCTGAGAGAGTTGATGAGATTGAGAAAGTTACAAAGCATGACATCATCGCATTTTGTACCTCTATTGCGGAGCAATTT
ACAGCTAAAACTGGCAAGTTTTTTCATTTTGGTGTTACATCTTCAGATATTATTGACTCTGCTCTTAGCCTACAAATTCG
TGATTCTATGAGCTATGTTATCAAAGATTTAGAGGCGCTTTGTGACTCGCTACTTACTAAGGCAGAAGAAACAAAAGAAA
TCATTACGATGGGTAGAAGTCATGGTATGTTTGCCGAACCGATGAGCTTTGGTCAGAAGTTTCTTGGTGCTTATGTTGAG
TTTAAGCGTAGGCTAAAAGATCTCAAAGATTTTCAAAAAGATGGTTTGACAGTACAGTTCTCAGGAGCTGTAGGTAACTA
TTGTATTTTAACTACAGAAGATGAGAAAAAAGCAGCTGATATTTTAGGTTTACCTGTTGAAGAAGTTTCTACTCAAGTTA
TCCCAAGAGATAGAATTGCTAAGTTGATATCTATCCACGGACTTATTGCTTCTGCTATAGAGAGATTAGCAGTTGAGATT
AGACATTTACATCGTAGTGATGTTTTTGAGGTATATGAAGGCTTCTCTAAAGGGCAAAAAGGCTCATCAACTATGCCGCA
TAAGAAAAACCCAATTTCTACTGAAAACTTAACAGGTATGGCAAGAATGCTAAGATCTCATGTATCTATAGCATTAGAGA
ATTGTGTGCTATGGCATGAGCGAGATATTTCTCACTCTTCAGCAGAGCGTTTTTATCTGCCAGATAACTTTGGTATTATG
GTTTATGCTTTACGTAGAATGAAAAATACTATCGATAATCTCGTCGTGCAAAGAGATATTATCGAAGATAGGGTTAGAAG
TACTAGTGCTTATTTATCAAGTTTTTACTTACATTTCTTGGTAGCAAATACGCCATTTATGCGTGAAGATTGCTACAAGA
TTGTTCAGCAAGTTGCTTTTGATCTTAAACAAGGAGAATCTTTCTCGAAGAAATTACAAAAAGTTATGCACGATGAGCAT
AATATTATTTTATATATTCCAGAGATGGATTTTGAGGGTATCAAGAAAACTTACCTAAAAGAAATTGATCATGTTTTTGA
TAGATCTGTTAAGGCGTAA

Upstream 100 bases:

>100_bases
AAATACTTCATCATGTATTGCCATGCTTGTGCTTTAGCTCAGTTTTAGCTTATATACATTAAGTGTGCTTGCGGGTGTTT
TATGAATATAAGGTAGTAAA

Downstream 100 bases:

>100_bases
ATATGAAAAAAATATTATCTTTTCTTTTTATTTATGTATAATGTTGTTAAGTTTGGATGCCAAGAGCGACCTGAAATAAG
TAGTGTAGAAGAGCCAACAA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MIKRYDIAEISKIWADENKYAKMLEVELAILEALEDRMVPKGTAAEIRARAQIRPERVDEIEKVTKHDIIAFCTSIAEQF
TAKTGKFFHFGVTSSDIIDSALSLQIRDSMSYVIKDLEALCDSLLTKAEETKEIITMGRSHGMFAEPMSFGQKFLGAYVE
FKRRLKDLKDFQKDGLTVQFSGAVGNYCILTTEDEKKAADILGLPVEEVSTQVIPRDRIAKLISIHGLIASAIERLAVEI
RHLHRSDVFEVYEGFSKGQKGSSTMPHKKNPISTENLTGMARMLRSHVSIALENCVLWHERDISHSSAERFYLPDNFGIM
VYALRRMKNTIDNLVVQRDIIEDRVRSTSAYLSSFYLHFLVANTPFMREDCYKIVQQVAFDLKQGESFSKKLQKVMHDEH
NIILYIPEMDFEGIKKTYLKEIDHVFDRSVKA

Sequences:

>Translated_432_residues
MIKRYDIAEISKIWADENKYAKMLEVELAILEALEDRMVPKGTAAEIRARAQIRPERVDEIEKVTKHDIIAFCTSIAEQF
TAKTGKFFHFGVTSSDIIDSALSLQIRDSMSYVIKDLEALCDSLLTKAEETKEIITMGRSHGMFAEPMSFGQKFLGAYVE
FKRRLKDLKDFQKDGLTVQFSGAVGNYCILTTEDEKKAADILGLPVEEVSTQVIPRDRIAKLISIHGLIASAIERLAVEI
RHLHRSDVFEVYEGFSKGQKGSSTMPHKKNPISTENLTGMARMLRSHVSIALENCVLWHERDISHSSAERFYLPDNFGIM
VYALRRMKNTIDNLVVQRDIIEDRVRSTSAYLSSFYLHFLVANTPFMREDCYKIVQQVAFDLKQGESFSKKLQKVMHDEH
NIILYIPEMDFEGIKKTYLKEIDHVFDRSVKA
>Mature_432_residues
MIKRYDIAEISKIWADENKYAKMLEVELAILEALEDRMVPKGTAAEIRARAQIRPERVDEIEKVTKHDIIAFCTSIAEQF
TAKTGKFFHFGVTSSDIIDSALSLQIRDSMSYVIKDLEALCDSLLTKAEETKEIITMGRSHGMFAEPMSFGQKFLGAYVE
FKRRLKDLKDFQKDGLTVQFSGAVGNYCILTTEDEKKAADILGLPVEEVSTQVIPRDRIAKLISIHGLIASAIERLAVEI
RHLHRSDVFEVYEGFSKGQKGSSTMPHKKNPISTENLTGMARMLRSHVSIALENCVLWHERDISHSSAERFYLPDNFGIM
VYALRRMKNTIDNLVVQRDIIEDRVRSTSAYLSSFYLHFLVANTPFMREDCYKIVQQVAFDLKQGESFSKKLQKVMHDEH
NIILYIPEMDFEGIKKTYLKEIDHVFDRSVKA

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=408, Percent_Identity=23.0392156862745, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI183227688, Length=374, Percent_Identity=22.9946524064171, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI1787376, Length=323, Percent_Identity=26.0061919504644, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17508577, Length=352, Percent_Identity=24.7159090909091, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI32564234, Length=295, Percent_Identity=24.0677966101695, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323391, Length=373, Percent_Identity=26.2734584450402, Blast_Score=103, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24647570, Length=404, Percent_Identity=23.5148514851485, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49439; Mature: 49439

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRYDIAEISKIWADENKYAKMLEVELAILEALEDRMVPKGTAAEIRARAQIRPERVDE
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHH
IEKVTKHDIIAFCTSIAEQFTAKTGKFFHFGVTSSDIIDSALSLQIRDSMSYVIKDLEAL
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
CDSLLTKAEETKEIITMGRSHGMFAEPMSFGQKFLGAYVEFKRRLKDLKDFQKDGLTVQF
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
SGAVGNYCILTTEDEKKAADILGLPVEEVSTQVIPRDRIAKLISIHGLIASAIERLAVEI
CCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RHLHRSDVFEVYEGFSKGQKGSSTMPHKKNPISTENLTGMARMLRSHVSIALENCVLWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
RDISHSSAERFYLPDNFGIMVYALRRMKNTIDNLVVQRDIIEDRVRSTSAYLSSFYLHFL
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VANTPFMREDCYKIVQQVAFDLKQGESFSKKLQKVMHDEHNIILYIPEMDFEGIKKTYLK
HHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHH
EIDHVFDRSVKA
HHHHHHHHCCCC
>Mature Secondary Structure
MIKRYDIAEISKIWADENKYAKMLEVELAILEALEDRMVPKGTAAEIRARAQIRPERVDE
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHH
IEKVTKHDIIAFCTSIAEQFTAKTGKFFHFGVTSSDIIDSALSLQIRDSMSYVIKDLEAL
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
CDSLLTKAEETKEIITMGRSHGMFAEPMSFGQKFLGAYVEFKRRLKDLKDFQKDGLTVQF
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
SGAVGNYCILTTEDEKKAADILGLPVEEVSTQVIPRDRIAKLISIHGLIASAIERLAVEI
CCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RHLHRSDVFEVYEGFSKGQKGSSTMPHKKNPISTENLTGMARMLRSHVSIALENCVLWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
RDISHSSAERFYLPDNFGIMVYALRRMKNTIDNLVVQRDIIEDRVRSTSAYLSSFYLHFL
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VANTPFMREDCYKIVQQVAFDLKQGESFSKKLQKVMHDEHNIILYIPEMDFEGIKKTYLK
HHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHH
EIDHVFDRSVKA
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571; 10673438 [H]