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The map label for this gene is glpF [H]

Identifier: 89257024

GI number: 89257024

Start: 1689356

End: 1690120

Strand: Reverse

Name: glpF [H]

Synonym: FTL_1755

Alternate gene names: 89257024

Gene position: 1690120-1689356 (Counterclockwise)

Preceding gene: 89257025

Following gene: 89257023

Centisome position: 89.14

GC content: 38.69

Gene sequence:

>765_bases
ATGTTAACAGCATGTATAGCAGAATTAATAGGCACAATGCTACTTATCTTATTGGGTAATGGTGTTGTTGCTGGAGTAGT
TTTAAATAAGACAAAAGCACATGATGGTGGCTGGATAGTTATTACATTTGCTTGGGGTCTAGCTGTTTTTATAGGTGTGT
TAGTTGCTGGACCGATTAGTGGTGCTCACTTGAATCCTGTTGTAACATTATCTTTAGCTCTTGCGGGTAAGTTTTCATGG
GCTTGGGTATTACCATTTATTATAAGTCAGATTATCGGAGCGATGCTTGGTCAACTATTAGTGTGGATTATGTATTATCC
TCATTATTCAGCAACTGATAATCCTGAACTAAAATTAGCGACTTGTTGCACATCACCAGCAATTAAGCATTTGCCAGCGA
ATTTTGCTAGTGAAGTAATTGGAACATTTGTTCTAGTACTAGCTATTTTGACTATGCATGGTGTTGTCTTACATGTCGGA
GACTCTAGTATAACAACAACTTATCCAGTAGATATGGGCGCTTTAGGGGGTATACCAGTAGCATTTGTGGTTGTTGTAAT
CGGCATGTCCTTAGGTGGGACTACAGGTTATGCAATTAATCTAGCTAGGGATTTTGGCCCAAGACTATTTCATGCTATCA
TGCCAATACAAGGAAAAGGCTCTTCACAATGGAGTTATGCTTGGGTACCGATACTTGGTCCAGTGTGTGGAAGTATAATT
GCCACAGCTTTTTATCTATTGTTAAAGGTAAAAGGGGTTTTTTAG

Upstream 100 bases:

>100_bases
GCTTGGTAAAGATGAGGTTTGGCAAAACCAGCAAATTGATAGTTTCATAGAATTTTCTAAAAACTTTAATGTTGAAGAGC
TGTATAAGTAGAGGAAGTTT

Downstream 100 bases:

>100_bases
ATAATGTGTGAAATAACTTTTATACCACTTTTAGCAGATGATGTTTTAAGATTGTTTATTTCTTTTATATTATTTTTAAG
TTGCGTGATTTTAAGTTATA

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MLTACIAELIGTMLLILLGNGVVAGVVLNKTKAHDGGWIVITFAWGLAVFIGVLVAGPISGAHLNPVVTLSLALAGKFSW
AWVLPFIISQIIGAMLGQLLVWIMYYPHYSATDNPELKLATCCTSPAIKHLPANFASEVIGTFVLVLAILTMHGVVLHVG
DSSITTTYPVDMGALGGIPVAFVVVVIGMSLGGTTGYAINLARDFGPRLFHAIMPIQGKGSSQWSYAWVPILGPVCGSII
ATAFYLLLKVKGVF

Sequences:

>Translated_254_residues
MLTACIAELIGTMLLILLGNGVVAGVVLNKTKAHDGGWIVITFAWGLAVFIGVLVAGPISGAHLNPVVTLSLALAGKFSW
AWVLPFIISQIIGAMLGQLLVWIMYYPHYSATDNPELKLATCCTSPAIKHLPANFASEVIGTFVLVLAILTMHGVVLHVG
DSSITTTYPVDMGALGGIPVAFVVVVIGMSLGGTTGYAINLARDFGPRLFHAIMPIQGKGSSQWSYAWVPILGPVCGSII
ATAFYLLLKVKGVF
>Mature_254_residues
MLTACIAELIGTMLLILLGNGVVAGVVLNKTKAHDGGWIVITFAWGLAVFIGVLVAGPISGAHLNPVVTLSLALAGKFSW
AWVLPFIISQIIGAMLGQLLVWIMYYPHYSATDNPELKLATCCTSPAIKHLPANFASEVIGTFVLVLAILTMHGVVLHVG
DSSITTTYPVDMGALGGIPVAFVVVVIGMSLGGTTGYAINLARDFGPRLFHAIMPIQGKGSSQWSYAWVPILGPVCGSII
ATAFYLLLKVKGVF

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=263, Percent_Identity=36.1216730038023, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI157266307, Length=259, Percent_Identity=33.2046332046332, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI22538420, Length=257, Percent_Identity=29.1828793774319, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI4502187, Length=260, Percent_Identity=28.8461538461538, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1790362, Length=259, Percent_Identity=34.3629343629344, Blast_Score=105, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71994009, Length=249, Percent_Identity=30.9236947791165, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17544068, Length=257, Percent_Identity=32.6848249027237, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17531429, Length=257, Percent_Identity=30.3501945525292, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17531431, Length=257, Percent_Identity=30.3501945525292, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17558372, Length=253, Percent_Identity=28.0632411067194, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32564052, Length=249, Percent_Identity=30.1204819277108, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17533613, Length=249, Percent_Identity=30.1204819277108, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI71992966, Length=258, Percent_Identity=29.0697674418605, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71992961, Length=251, Percent_Identity=28.6852589641434, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71993722, Length=257, Percent_Identity=28.4046692607004, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321054, Length=221, Percent_Identity=32.1266968325792, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322985, Length=284, Percent_Identity=25.7042253521127, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 26717; Mature: 26717

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTACIAELIGTMLLILLGNGVVAGVVLNKTKAHDGGWIVITFAWGLAVFIGVLVAGPIS
CHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHCCCC
GAHLNPVVTLSLALAGKFSWAWVLPFIISQIIGAMLGQLLVWIMYYPHYSATDNPELKLA
CCCCCHHHEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
TCCTSPAIKHLPANFASEVIGTFVLVLAILTMHGVVLHVGDSSITTTYPVDMGALGGIPV
EECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCCCCCCHHH
AFVVVVIGMSLGGTTGYAINLARDFGPRLFHAIMPIQGKGSSQWSYAWVPILGPVCGSII
HHHHHHHHHHCCCCCCEEEEEHHHHCHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHH
ATAFYLLLKVKGVF
HHHHHHHHHHHCCC
>Mature Secondary Structure
MLTACIAELIGTMLLILLGNGVVAGVVLNKTKAHDGGWIVITFAWGLAVFIGVLVAGPIS
CHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHCCCC
GAHLNPVVTLSLALAGKFSWAWVLPFIISQIIGAMLGQLLVWIMYYPHYSATDNPELKLA
CCCCCHHHEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
TCCTSPAIKHLPANFASEVIGTFVLVLAILTMHGVVLHVGDSSITTTYPVDMGALGGIPV
EECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCCCCCCHHH
AFVVVVIGMSLGGTTGYAINLARDFGPRLFHAIMPIQGKGSSQWSYAWVPILGPVCGSII
HHHHHHHHHHCCCCCCEEEEEHHHHCHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHH
ATAFYLLLKVKGVF
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]