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The map label for this gene is dus2 [H]

Identifier: 89256833

GI number: 89256833

Start: 1479827

End: 1480810

Strand: Direct

Name: dus2 [H]

Synonym: FTL_1549

Alternate gene names: 89256833

Gene position: 1479827-1480810 (Clockwise)

Preceding gene: 89256832

Following gene: 89256835

Centisome position: 78.05

GC content: 32.22

Gene sequence:

>984_bases
ATGAAAATAACAACTAGAAAAATTTGTATAGCACCAATGCTTGACTGGACTGATAGGCACTATCGTTACATGATGCGTCT
AATCACCAGGCATACAATGTTATATACTGAAATGGTTACTTTAAACGCAATCTTACATGGTAATAAAGAATTTTTGCTTA
AATATAATCCTCAAGAAAATCCTGTGACACTACAGTTAGGTGGTTGCGTTCCAAAAGACTTTATCAGTTGTGGCAAGCTT
GCCGAAGAACTTGACTATAATGAAATTAACATAAATGTTGGCTGCCCAAGTGAAAGAGTTAAAAAAGGTAATTTTGGCTT
ATCATTAATGGCAGAGCCAGAGCTTGTAGCAGACTGTGTCAAAGCTATGAAAGATAACCTAAATATCCCAATTTCAGTTA
AATGTCGTATTGGCTATGATCATAATGATAGCTATGAAGAACTATATAATTTTGTTGATAAGCAAGTTCAAGCAGGAATA
GATTACCTTTGTATTCATGCACGTAAAGGTTGGCTTAGCGGTCTATCACCAAAAGAAAATCGTACCATACCTGAGCTTAA
ATATGATGTTGTCTACAGTATCAAAAAAGATTTCCCAAACCTTGAACTAGGGATTAATGGCGGTATTACAACAATTCAAG
AAATTCAACAGCACCTTAAATATGTGGATAGTGTTATGATAGGTAGAGAAGCATATCATAACCCAATGTTATTTAAAGAT
TTTGATAATATTTTCTATAATCAACCTACTACTAATATATCAGCATTAGAAATTGCTTATAAGATGGCTGAATATATAAA
AGACCAAATGGATAATGATCCATATATAAAACTTAACAATATAACTAAACATACCTTAAACCTATTTAATGGTTTGCCTA
ATGCTAAAGTATATCGTCGTTATCTAAGCGAAAATGCCACAAAAAAAGAAGCCAATGTAGATACCTTTTTAACAGCTTTA
GAAGTTTTTGAGAATAAATTATAG

Upstream 100 bases:

>100_bases
TGAGAAATATCGTCCACAAATTGATAAAATCATTCGCCGCTTAACTTTTGATGATGATCCTTTATAATTTTATAACAACT
TAAATCTCGTAGCTTTTAAA

Downstream 100 bases:

>100_bases
TCTAATTTACTTGATATTATTAAGCTGTGCTTGAATATTAGTAGCAACCCATTTTGCTGCTTGTTGATAATTATCTGTAA
TAGCATTAGTCTGTTTGTCT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKITTRKICIAPMLDWTDRHYRYMMRLITRHTMLYTEMVTLNAILHGNKEFLLKYNPQENPVTLQLGGCVPKDFISCGKL
AEELDYNEININVGCPSERVKKGNFGLSLMAEPELVADCVKAMKDNLNIPISVKCRIGYDHNDSYEELYNFVDKQVQAGI
DYLCIHARKGWLSGLSPKENRTIPELKYDVVYSIKKDFPNLELGINGGITTIQEIQQHLKYVDSVMIGREAYHNPMLFKD
FDNIFYNQPTTNISALEIAYKMAEYIKDQMDNDPYIKLNNITKHTLNLFNGLPNAKVYRRYLSENATKKEANVDTFLTAL
EVFENKL

Sequences:

>Translated_327_residues
MKITTRKICIAPMLDWTDRHYRYMMRLITRHTMLYTEMVTLNAILHGNKEFLLKYNPQENPVTLQLGGCVPKDFISCGKL
AEELDYNEININVGCPSERVKKGNFGLSLMAEPELVADCVKAMKDNLNIPISVKCRIGYDHNDSYEELYNFVDKQVQAGI
DYLCIHARKGWLSGLSPKENRTIPELKYDVVYSIKKDFPNLELGINGGITTIQEIQQHLKYVDSVMIGREAYHNPMLFKD
FDNIFYNQPTTNISALEIAYKMAEYIKDQMDNDPYIKLNNITKHTLNLFNGLPNAKVYRRYLSENATKKEANVDTFLTAL
EVFENKL
>Mature_327_residues
MKITTRKICIAPMLDWTDRHYRYMMRLITRHTMLYTEMVTLNAILHGNKEFLLKYNPQENPVTLQLGGCVPKDFISCGKL
AEELDYNEININVGCPSERVKKGNFGLSLMAEPELVADCVKAMKDNLNIPISVKCRIGYDHNDSYEELYNFVDKQVQAGI
DYLCIHARKGWLSGLSPKENRTIPELKYDVVYSIKKDFPNLELGINGGITTIQEIQQHLKYVDSVMIGREAYHNPMLFKD
FDNIFYNQPTTNISALEIAYKMAEYIKDQMDNDPYIKLNNITKHTLNLFNGLPNAKVYRRYLSENATKKEANVDTFLTAL
EVFENKL

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=243, Percent_Identity=26.3374485596708, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI145693211, Length=321, Percent_Identity=49.5327102803738, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI1788462, Length=255, Percent_Identity=27.4509803921569, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1789660, Length=243, Percent_Identity=25.1028806584362, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17507177, Length=268, Percent_Identity=24.2537313432836, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=240, Percent_Identity=23.3333333333333, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24580595, Length=238, Percent_Identity=24.7899159663866, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19920448, Length=238, Percent_Identity=24.7899159663866, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37763; Mature: 37763

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITTRKICIAPMLDWTDRHYRYMMRLITRHTMLYTEMVTLNAILHGNKEFLLKYNPQEN
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
PVTLQLGGCVPKDFISCGKLAEELDYNEININVGCPSERVKKGNFGLSLMAEPELVADCV
CEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEEEECHHHHHHHH
KAMKDNLNIPISVKCRIGYDHNDSYEELYNFVDKQVQAGIDYLCIHARKGWLSGLSPKEN
HHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHCCCCCCCC
RTIPELKYDVVYSIKKDFPNLELGINGGITTIQEIQQHLKYVDSVMIGREAYHNPMLFKD
CCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
FDNIFYNQPTTNISALEIAYKMAEYIKDQMDNDPYIKLNNITKHTLNLFNGLPNAKVYRR
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCHHHHHH
YLSENATKKEANVDTFLTALEVFENKL
HHHCCCCCHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKITTRKICIAPMLDWTDRHYRYMMRLITRHTMLYTEMVTLNAILHGNKEFLLKYNPQEN
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
PVTLQLGGCVPKDFISCGKLAEELDYNEININVGCPSERVKKGNFGLSLMAEPELVADCV
CEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEEEECHHHHHHHH
KAMKDNLNIPISVKCRIGYDHNDSYEELYNFVDKQVQAGIDYLCIHARKGWLSGLSPKEN
HHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHCCCCCCCC
RTIPELKYDVVYSIKKDFPNLELGINGGITTIQEIQQHLKYVDSVMIGREAYHNPMLFKD
CCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
FDNIFYNQPTTNISALEIAYKMAEYIKDQMDNDPYIKLNNITKHTLNLFNGLPNAKVYRR
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCHHHHHH
YLSENATKKEANVDTFLTALEVFENKL
HHHCCCCCHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]