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The map label for this gene is murE [H]

Identifier: 89255900

GI number: 89255900

Start: 475477

End: 476940

Strand: Direct

Name: murE [H]

Synonym: FTL_0490

Alternate gene names: 89255900

Gene position: 475477-476940 (Clockwise)

Preceding gene: 89255899

Following gene: 89255901

Centisome position: 25.08

GC content: 29.3

Gene sequence:

>1464_bases
ATGAAACGTATAAACCAAATATTAAAATTTCTAGATATTAAAACTTTATCTCAAAATAATTTTCAAATTGAGTCTCTATA
TTTAGATAGTCGTAAATGTGATAAAAAATCAGTGTTTGTAGCTCTCAAAGGTCTATCAACTGATGGTAATAAATATATTG
ATAGTGTTTTAGCTAAAGGTGTCAGTTTAGTATTAACAGATAGTCATGAATTTGAAGACCAGAAACGTGTTTTTTATATT
CAAAATTTAAAAGAGAAACTATCGACCCTTGCAAAATGGTTTTATGATTATGAAAAACCACAAAATATAATAGGTATAAC
TGGCACAAATGGTAAAACTTCTATCTCTAGTTATATTGCTCAATTTTTAGATTTAAGTGCACAAAAAACGCTACTTTTAG
GTACAAATGGCAATGGCATTTATCCTGACTTAAGAGAGAGTACACATACGACATTAGATATATTGTCTTTGTATAAAACT
ATTTCATATTATAAAAATTATCAAAATCTTGTGATGGAAGTATCTTCTCATTCATTAGACCAAAAGCGTGTTGAAGGCTT
AGATTTTGATGTTGCTATATTTAGTAATCTTAGCCATGATCATCTTGATTATCATAAGACTATGGATAATTATTTTGAAG
CTAAGGCAAAATTGTTTCAATTCAAAAGACTTAAAAAAGCTATAATAAATATAGATGATGAGTATGGTCAAAGACTGTGT
AATATGACACAGGTACCGGTTATTACAGTAAGTCTAAAATCTGAATATGCTGATGTCTATATTAAGCCTAAATCTATAGA
AGGTATTAAAACTAAGTTTGAAATGTTTTTTAATGGTAGGTTTGTTGGTGAATATACAACTAAGCTTATCGGCGATTTTA
ACTTGATGAATTTGGGCTTGAGCTTTGCATCACTTGATGGTGTGTTAGATTGGGATTGTATATTAGATCTAGGAAATATC
AAACCAGTGAAAGGTCGAATGGAAGTGATAGAGCTAGATAATGATATAAAGATAGTTATTGATTATGCGCATACTCCAGA
TGCTTTAGAAAAGGCTTTACAAACCTTAAATAGCTACAATCCAAATAATTTATGGTGTATTTTTGGCTGTGGTGGTAATA
GAGATACTTCCAAAAGACCTATAATGGCACAAATAGCGGAAGAATATGCTAGCAAAATAGTAATTACAGAAGATAATAAT
CGTTTTGAGAGTATTGAAAATATATTCAGTGATATTAGAAAAGGATTTAAGTATCCGCAAAAGCATATTTTTATCACCTC
TCGAGAACAAGCTATTAGGTTTAGTATAGAAAATGCTAATCAAGGAGATATTATTTTATTAGCAAGTAAAGGGCATGAGT
GTTACCTTGATAAAAATGGTGCTAAAGAGTATTTTGATGAACGAGAAATCATAAAAAAATATGCCGGAGCATTCTCTTTG
AATATGATAAATAAAGAAATATAA

Upstream 100 bases:

>100_bases
TGGTGCCTATAAAACTCGCAAGCTCAGACAGTTGTAGTCACTGGATCAATGACTTTGACATAAGCTTGCCACAAAGTGCT
TTAATGTGGTGAAAATTAAA

Downstream 100 bases:

>100_bases
TTTATCTTTTAGATTGAGATTATGAGAATGATTAATAAAAAAGAGCTGACCTTATCAATGGTTATGATTTTATTAGCTAG
TTGCTCATCTGTTGAATTAA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2,6- diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MKRINQILKFLDIKTLSQNNFQIESLYLDSRKCDKKSVFVALKGLSTDGNKYIDSVLAKGVSLVLTDSHEFEDQKRVFYI
QNLKEKLSTLAKWFYDYEKPQNIIGITGTNGKTSISSYIAQFLDLSAQKTLLLGTNGNGIYPDLRESTHTTLDILSLYKT
ISYYKNYQNLVMEVSSHSLDQKRVEGLDFDVAIFSNLSHDHLDYHKTMDNYFEAKAKLFQFKRLKKAIINIDDEYGQRLC
NMTQVPVITVSLKSEYADVYIKPKSIEGIKTKFEMFFNGRFVGEYTTKLIGDFNLMNLGLSFASLDGVLDWDCILDLGNI
KPVKGRMEVIELDNDIKIVIDYAHTPDALEKALQTLNSYNPNNLWCIFGCGGNRDTSKRPIMAQIAEEYASKIVITEDNN
RFESIENIFSDIRKGFKYPQKHIFITSREQAIRFSIENANQGDIILLASKGHECYLDKNGAKEYFDEREIIKKYAGAFSL
NMINKEI

Sequences:

>Translated_487_residues
MKRINQILKFLDIKTLSQNNFQIESLYLDSRKCDKKSVFVALKGLSTDGNKYIDSVLAKGVSLVLTDSHEFEDQKRVFYI
QNLKEKLSTLAKWFYDYEKPQNIIGITGTNGKTSISSYIAQFLDLSAQKTLLLGTNGNGIYPDLRESTHTTLDILSLYKT
ISYYKNYQNLVMEVSSHSLDQKRVEGLDFDVAIFSNLSHDHLDYHKTMDNYFEAKAKLFQFKRLKKAIINIDDEYGQRLC
NMTQVPVITVSLKSEYADVYIKPKSIEGIKTKFEMFFNGRFVGEYTTKLIGDFNLMNLGLSFASLDGVLDWDCILDLGNI
KPVKGRMEVIELDNDIKIVIDYAHTPDALEKALQTLNSYNPNNLWCIFGCGGNRDTSKRPIMAQIAEEYASKIVITEDNN
RFESIENIFSDIRKGFKYPQKHIFITSREQAIRFSIENANQGDIILLASKGHECYLDKNGAKEYFDEREIIKKYAGAFSL
NMINKEI
>Mature_487_residues
MKRINQILKFLDIKTLSQNNFQIESLYLDSRKCDKKSVFVALKGLSTDGNKYIDSVLAKGVSLVLTDSHEFEDQKRVFYI
QNLKEKLSTLAKWFYDYEKPQNIIGITGTNGKTSISSYIAQFLDLSAQKTLLLGTNGNGIYPDLRESTHTTLDILSLYKT
ISYYKNYQNLVMEVSSHSLDQKRVEGLDFDVAIFSNLSHDHLDYHKTMDNYFEAKAKLFQFKRLKKAIINIDDEYGQRLC
NMTQVPVITVSLKSEYADVYIKPKSIEGIKTKFEMFFNGRFVGEYTTKLIGDFNLMNLGLSFASLDGVLDWDCILDLGNI
KPVKGRMEVIELDNDIKIVIDYAHTPDALEKALQTLNSYNPNNLWCIFGCGGNRDTSKRPIMAQIAEEYASKIVITEDNN
RFESIENIFSDIRKGFKYPQKHIFITSREQAIRFSIENANQGDIILLASKGHECYLDKNGAKEYFDEREIIKKYAGAFSL
NMINKEI

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=485, Percent_Identity=34.8453608247423, Blast_Score=244, Evalue=6e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 55849; Mature: 55849

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRINQILKFLDIKTLSQNNFQIESLYLDSRKCDKKSVFVALKGLSTDGNKYIDSVLAKG
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHCC
VSLVLTDSHEFEDQKRVFYIQNLKEKLSTLAKWFYDYEKPQNIIGITGTNGKTSISSYIA
EEEEEECCCCCCCCHHEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHH
QFLDLSAQKTLLLGTNGNGIYPDLRESTHTTLDILSLYKTISYYKNYQNLVMEVSSHSLD
HHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QKRVEGLDFDVAIFSNLSHDHLDYHKTMDNYFEAKAKLFQFKRLKKAIINIDDEYGQRLC
HHHHCCCCEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
NMTQVPVITVSLKSEYADVYIKPKSIEGIKTKFEMFFNGRFVGEYTTKLIGDFNLMNLGL
CCCCCCEEEEEECCCCCEEEEECCCCCHHHHEEEHEECCEEEHHHHHHHHCCCCHHHHCC
SFASLDGVLDWDCILDLGNIKPVKGRMEVIELDNDIKIVIDYAHTPDALEKALQTLNSYN
HHHHHCCCCCHHHEEECCCCCCCCCCEEEEEECCCEEEEEEECCCCHHHHHHHHHHHCCC
PNNLWCIFGCGGNRDTSKRPIMAQIAEEYASKIVITEDNNRFESIENIFSDIRKGFKYPQ
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHCCCC
KHIFITSREQAIRFSIENANQGDIILLASKGHECYLDKNGAKEYFDEREIIKKYAGAFSL
CEEEEEECCCEEEEEECCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCEEE
NMINKEI
EHHCCCC
>Mature Secondary Structure
MKRINQILKFLDIKTLSQNNFQIESLYLDSRKCDKKSVFVALKGLSTDGNKYIDSVLAKG
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHCC
VSLVLTDSHEFEDQKRVFYIQNLKEKLSTLAKWFYDYEKPQNIIGITGTNGKTSISSYIA
EEEEEECCCCCCCCHHEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHH
QFLDLSAQKTLLLGTNGNGIYPDLRESTHTTLDILSLYKTISYYKNYQNLVMEVSSHSLD
HHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QKRVEGLDFDVAIFSNLSHDHLDYHKTMDNYFEAKAKLFQFKRLKKAIINIDDEYGQRLC
HHHHCCCCEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
NMTQVPVITVSLKSEYADVYIKPKSIEGIKTKFEMFFNGRFVGEYTTKLIGDFNLMNLGL
CCCCCCEEEEEECCCCCEEEEECCCCCHHHHEEEHEECCEEEHHHHHHHHCCCCHHHHCC
SFASLDGVLDWDCILDLGNIKPVKGRMEVIELDNDIKIVIDYAHTPDALEKALQTLNSYN
HHHHHCCCCCHHHEEECCCCCCCCCCEEEEEECCCEEEEEEECCCCHHHHHHHHHHHCCC
PNNLWCIFGCGGNRDTSKRPIMAQIAEEYASKIVITEDNNRFESIENIFSDIRKGFKYPQ
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHCCCC
KHIFITSREQAIRFSIENANQGDIILLASKGHECYLDKNGAKEYFDEREIIKKYAGAFSL
CEEEEEECCCEEEEEECCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCEEE
NMINKEI
EHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]