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The map label for this gene is tolB [H]

Identifier: 89255760

GI number: 89255760

Start: 316902

End: 318209

Strand: Direct

Name: tolB [H]

Synonym: FTL_0334

Alternate gene names: 89255760

Gene position: 316902-318209 (Clockwise)

Preceding gene: 89255759

Following gene: 89255761

Centisome position: 16.71

GC content: 32.26

Gene sequence:

>1308_bases
ATGAGGAAAATCATTGCAGGCGTTTTTATATTTGTTTTTTTGATCTCTAATCTATATGCAGACTTGGTTGCAGAGGTGAC
TACTGGAGTAATTCAAAAGCCGTTAGTTACGGTTGTTAGTGATAATGTTGTTGATCAGTTTCCGCAGCAGGTAAATTCTG
TAATGGTTGCAGACTTAAACCATAATGCTAAATTACAAGCTAATGATACAATTAAATATGAAATTAAGCAAAAGCAAAAT
ATTCCTTGGAAGAGCCTAAAATCTGACTATGTTGTATTAACAAAATATACGAATAACTCATATAATAATTATACTGTAGA
AGTGCAGATTCTAAAGAGAAATGACACAAGTTATTTACAAGCGATTACTTATAAAAATATAAATGTTTCTCTAATGAGAA
CATTGGCACATAAGATTTCTAATTATGTTTATCAAAAGCTTACGGGCAATCAGGGCTTCTTTTTAACTAAGCTTGCATAT
GTCAAAGTAAGTAATCTATATGCAAGATATGGTAGGTTATATGAGCTTATTATATCTGACTATGATGGTTATAATAAGCA
TGTTGTACTAAGACAAACTGATAACCCAATAGCAACGCCTTCATGGTCAAATGATGGTCGTTATATTGTTTATTCTAGTT
ATAGTGGTGGTAGTATGGGAGTATATACTTTAGAAATAGCTACTGGTAAGGTAACACGTATAACTAACTATAAAGGTATA
AATAGCTCACCTTCGTTCTCACCAGATGGTAAAGAAATTGCCTTAGCGCTATCAAAAGGCTATTCTGATCAGACTAATAT
CTACATCATGAACTTAGCTACAAAAGCACTAAAGAGAATCACTATAAATGGTATAAATACAGCACCAAAGTTTTCTCCAA
ATGGTCAAAGCATAGTGTTTACATCTGATAGAGAAGGCAGACCAAATATTTATGTAGCATCAGTAAACTCTAAATATCCA
CAATCATCAATATTAAGTACAAAAATACATCAGGCATATGAGCCAAACTATACTCCAGATGGCAAAAATATAGTATTTAT
GAATCAAAGCTCACGCACAAGCGGTACGCAAATTGCTGATTTTAATTTAGTTAATGGAAGTGTAACAAATATTACAAATG
GTAAAGCAGACAGTTCACCTACAGTTTCGCCATATGGTGATATGGTTGCTTATATATCAACCAACACTAGAGGTTATAGC
TCATTAGATATGGTATCATTAGATGGTGATAATCATTTCAATATTGAGACTGCTGATAATGGTAATATTTTAATACAATC
ACCAAGTTGGTCGCCAAAAAACTTTTAA

Upstream 100 bases:

>100_bases
AAAATACTACGCCACCTACAATTACAAATAATGTGGCAAGAAAAACTATACAAACAGAAAATATAAGCTTTATATTTAAA
CAAAGTTAATGAGGAATGGT

Downstream 100 bases:

>100_bases
GGAATGAGTATGCAAGTTGCTATTAAAATATATTTGATTGGTTTTTTCGCTTTGTTAGTTACAAGCTGTGCATGGTATAA
AGGACCTAGTTTAATGCCTA

Product: TolB protein precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MRKIIAGVFIFVFLISNLYADLVAEVTTGVIQKPLVTVVSDNVVDQFPQQVNSVMVADLNHNAKLQANDTIKYEIKQKQN
IPWKSLKSDYVVLTKYTNNSYNNYTVEVQILKRNDTSYLQAITYKNINVSLMRTLAHKISNYVYQKLTGNQGFFLTKLAY
VKVSNLYARYGRLYELIISDYDGYNKHVVLRQTDNPIATPSWSNDGRYIVYSSYSGGSMGVYTLEIATGKVTRITNYKGI
NSSPSFSPDGKEIALALSKGYSDQTNIYIMNLATKALKRITINGINTAPKFSPNGQSIVFTSDREGRPNIYVASVNSKYP
QSSILSTKIHQAYEPNYTPDGKNIVFMNQSSRTSGTQIADFNLVNGSVTNITNGKADSSPTVSPYGDMVAYISTNTRGYS
SLDMVSLDGDNHFNIETADNGNILIQSPSWSPKNF

Sequences:

>Translated_435_residues
MRKIIAGVFIFVFLISNLYADLVAEVTTGVIQKPLVTVVSDNVVDQFPQQVNSVMVADLNHNAKLQANDTIKYEIKQKQN
IPWKSLKSDYVVLTKYTNNSYNNYTVEVQILKRNDTSYLQAITYKNINVSLMRTLAHKISNYVYQKLTGNQGFFLTKLAY
VKVSNLYARYGRLYELIISDYDGYNKHVVLRQTDNPIATPSWSNDGRYIVYSSYSGGSMGVYTLEIATGKVTRITNYKGI
NSSPSFSPDGKEIALALSKGYSDQTNIYIMNLATKALKRITINGINTAPKFSPNGQSIVFTSDREGRPNIYVASVNSKYP
QSSILSTKIHQAYEPNYTPDGKNIVFMNQSSRTSGTQIADFNLVNGSVTNITNGKADSSPTVSPYGDMVAYISTNTRGYS
SLDMVSLDGDNHFNIETADNGNILIQSPSWSPKNF
>Mature_435_residues
MRKIIAGVFIFVFLISNLYADLVAEVTTGVIQKPLVTVVSDNVVDQFPQQVNSVMVADLNHNAKLQANDTIKYEIKQKQN
IPWKSLKSDYVVLTKYTNNSYNNYTVEVQILKRNDTSYLQAITYKNINVSLMRTLAHKISNYVYQKLTGNQGFFLTKLAY
VKVSNLYARYGRLYELIISDYDGYNKHVVLRQTDNPIATPSWSNDGRYIVYSSYSGGSMGVYTLEIATGKVTRITNYKGI
NSSPSFSPDGKEIALALSKGYSDQTNIYIMNLATKALKRITINGINTAPKFSPNGQSIVFTSDREGRPNIYVASVNSKYP
QSSILSTKIHQAYEPNYTPDGKNIVFMNQSSRTSGTQIADFNLVNGSVTNITNGKADSSPTVSPYGDMVAYISTNTRGYS
SLDMVSLDGDNHFNIETADNGNILIQSPSWSPKNF

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=446, Percent_Identity=31.390134529148, Blast_Score=199, Evalue=4e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 48453; Mature: 48453

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIIAGVFIFVFLISNLYADLVAEVTTGVIQKPLVTVVSDNVVDQFPQQVNSVMVADLN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCHHHHHHHHHHCCEEEEECC
HNAKLQANDTIKYEIKQKQNIPWKSLKSDYVVLTKYTNNSYNNYTVEVQILKRNDTSYLQ
CCCEEEECCEEEEEEECCCCCCHHHCCCCEEEEEEEECCCCCEEEEEEEEEECCCCCEEE
AITYKNINVSLMRTLAHKISNYVYQKLTGNQGFFLTKLAYVKVSNLYARYGRLYELIISD
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHEEEEECC
YDGYNKHVVLRQTDNPIATPSWSNDGRYIVYSSYSGGSMGVYTLEIATGKVTRITNYKGI
CCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEEEECCCEEEEEECCCC
NSSPSFSPDGKEIALALSKGYSDQTNIYIMNLATKALKRITINGINTAPKFSPNGQSIVF
CCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEHHHHHHEEEEECCCCCCCCCCCCCEEEE
TSDREGRPNIYVASVNSKYPQSSILSTKIHQAYEPNYTPDGKNIVFMNQSSRTSGTQIAD
ECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEEE
FNLVNGSVTNITNGKADSSPTVSPYGDMVAYISTNTRGYSSLDMVSLDGDNHFNIETADN
EEEECCCEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCEEEEEECCC
GNILIQSPSWSPKNF
CCEEEECCCCCCCCC
>Mature Secondary Structure
MRKIIAGVFIFVFLISNLYADLVAEVTTGVIQKPLVTVVSDNVVDQFPQQVNSVMVADLN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCHHHHHHHHHHCCEEEEECC
HNAKLQANDTIKYEIKQKQNIPWKSLKSDYVVLTKYTNNSYNNYTVEVQILKRNDTSYLQ
CCCEEEECCEEEEEEECCCCCCHHHCCCCEEEEEEEECCCCCEEEEEEEEEECCCCCEEE
AITYKNINVSLMRTLAHKISNYVYQKLTGNQGFFLTKLAYVKVSNLYARYGRLYELIISD
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHEEEEECC
YDGYNKHVVLRQTDNPIATPSWSNDGRYIVYSSYSGGSMGVYTLEIATGKVTRITNYKGI
CCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEEEECCCEEEEEECCCC
NSSPSFSPDGKEIALALSKGYSDQTNIYIMNLATKALKRITINGINTAPKFSPNGQSIVF
CCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEHHHHHHEEEEECCCCCCCCCCCCCEEEE
TSDREGRPNIYVASVNSKYPQSSILSTKIHQAYEPNYTPDGKNIVFMNQSSRTSGTQIAD
ECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEEE
FNLVNGSVTNITNGKADSSPTVSPYGDMVAYISTNTRGYSSLDMVSLDGDNHFNIETADN
EEEECCCEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCEEEEEECCC
GNILIQSPSWSPKNF
CCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA