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The map label for this gene is gltX [H]

Identifier: 89255652

GI number: 89255652

Start: 218246

End: 219652

Strand: Direct

Name: gltX [H]

Synonym: FTL_0218

Alternate gene names: 89255652

Gene position: 218246-219652 (Clockwise)

Preceding gene: 89255651

Following gene: 89255653

Centisome position: 11.51

GC content: 35.18

Gene sequence:

>1407_bases
ATGATTACAACAAGATTTGCACCAAGTCCAACAGGGTTTTTACATGTTGGTGGAGTGCGTACTGCATTATTTAGCTGGTT
ATATGCCAAAAATAACAATGGTAAATTTATTCTAAGAATAGAAGATACTGATTTGGAGAGATCTACTCAAGAGGCGGTCG
ATGCTATTTTAGATGGAATGAGTTGGTTGGGGCTAAAAAATGATGGTGAGATCTACTATCAAACAAAGCGCTTTGATAGA
TATAAAGAAGTGATACAAGAACTTATTGCGGATGGTAAAGCTTACTACTGTAGCTGTTCTAAAGAAAGACTAGAAGAGTT
AAGAGAGTATCAGCAAGCAAATAATCTTAAAACTGGCTATGATGGTAAATGTCGTGATGCAAACTATATTCCACAACAAG
GTGAGAGTTATGTAGTACGTTTTAAGAATCCTCAAGATGGAGTGGTCAGTTGGGATGATGCTGTCAAAGGTCGAATCTCA
ATTTCAAATCATGAGCTTGATGATATGATTATCCAAAGAGCAGATGGTTCACCAACATATAATTTCTGTGTTGTTGTTGA
TGATATTGATATGGCTATTACACATATTATTCGAGGCGATGATCATGTTAATAATACCCCTAAACAAATCAATATATACA
AAGCTCTAAATGCTAATGTGCCGGTATTTGCCCATGTACCAATGATTCTTGGTCCAGATGGGGCAAAGTTGTCTAAGCGT
CATGGTGCTGTTAATGTTATGCAGTACCGTGAAGATGGCTATTTACCTCAGGCGATACTTAATTATCTTGTTAGGCTTGG
TTGGTCGCATGGTGATCAAGAGATTTTTTCTATCGAAGAAATGATAAAAGCTTTTAATTTAGAGCACATTAACGCATCAC
CATCACGTTTTGATTTTGAAAAACTTAAGTGGCTGAACAAACATTATATCAAAGAGTCTAAGTTTGATGATATTCAAACA
GAAGTTGAGTATCACTTTGCTAAAATTGGTTTAGATATTAGTAATGGTCCAGATTTAAAAGAGCTTGTAGCTGTGATGGC
AGAAAAAGTTGATACATTAGTTGAACTAGCAGAAAAATCTAGTTATTTTTATAGTGATGATATCAGTTATGATGAAAATG
CTGTAAAAAAACATATCAAAGCTTCAACGGGTGAGATATTTGTTAAGTTATTAGAAAACTTTGAAGCTTTGGATGCTCAG
CAATGGCAAGATCCTGATGTTTTATATAATATAGTTAGTACAACAGCAGAGCAGTGCCAAGTTGGTATGGGTAAAGTTGG
TATGCCATTGCGTGTGGCTATTACTAGTTCTGGCCAATCACCAGATATTGGTATTACTCTAAAACTTCTTGGTAAAAATA
AAGTAGTTGCTAGACTTACTAAAGCACTTGAAGAGTTATGCAAGTAA

Upstream 100 bases:

>100_bases
ATTAGATCTATTAACACTTATTAATCGTTTGCTTTAATCCTTGAAAGAAACTCGTTTCTAGTTTAATCTTTAAGCAATTT
TATATTCAAGGTGTTTATAT

Downstream 100 bases:

>100_bases
TGAAAATTAGAAAATTAAGCCTAATTATGTCGGTTTTGTTGTTTGCTACTGCAACTTACGCGAATAACCATTTTGGTAAT
AACTTAGCTGGCTGCAGTTT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MITTRFAPSPTGFLHVGGVRTALFSWLYAKNNNGKFILRIEDTDLERSTQEAVDAILDGMSWLGLKNDGEIYYQTKRFDR
YKEVIQELIADGKAYYCSCSKERLEELREYQQANNLKTGYDGKCRDANYIPQQGESYVVRFKNPQDGVVSWDDAVKGRIS
ISNHELDDMIIQRADGSPTYNFCVVVDDIDMAITHIIRGDDHVNNTPKQINIYKALNANVPVFAHVPMILGPDGAKLSKR
HGAVNVMQYREDGYLPQAILNYLVRLGWSHGDQEIFSIEEMIKAFNLEHINASPSRFDFEKLKWLNKHYIKESKFDDIQT
EVEYHFAKIGLDISNGPDLKELVAVMAEKVDTLVELAEKSSYFYSDDISYDENAVKKHIKASTGEIFVKLLENFEALDAQ
QWQDPDVLYNIVSTTAEQCQVGMGKVGMPLRVAITSSGQSPDIGITLKLLGKNKVVARLTKALEELCK

Sequences:

>Translated_468_residues
MITTRFAPSPTGFLHVGGVRTALFSWLYAKNNNGKFILRIEDTDLERSTQEAVDAILDGMSWLGLKNDGEIYYQTKRFDR
YKEVIQELIADGKAYYCSCSKERLEELREYQQANNLKTGYDGKCRDANYIPQQGESYVVRFKNPQDGVVSWDDAVKGRIS
ISNHELDDMIIQRADGSPTYNFCVVVDDIDMAITHIIRGDDHVNNTPKQINIYKALNANVPVFAHVPMILGPDGAKLSKR
HGAVNVMQYREDGYLPQAILNYLVRLGWSHGDQEIFSIEEMIKAFNLEHINASPSRFDFEKLKWLNKHYIKESKFDDIQT
EVEYHFAKIGLDISNGPDLKELVAVMAEKVDTLVELAEKSSYFYSDDISYDENAVKKHIKASTGEIFVKLLENFEALDAQ
QWQDPDVLYNIVSTTAEQCQVGMGKVGMPLRVAITSSGQSPDIGITLKLLGKNKVVARLTKALEELCK
>Mature_468_residues
MITTRFAPSPTGFLHVGGVRTALFSWLYAKNNNGKFILRIEDTDLERSTQEAVDAILDGMSWLGLKNDGEIYYQTKRFDR
YKEVIQELIADGKAYYCSCSKERLEELREYQQANNLKTGYDGKCRDANYIPQQGESYVVRFKNPQDGVVSWDDAVKGRIS
ISNHELDDMIIQRADGSPTYNFCVVVDDIDMAITHIIRGDDHVNNTPKQINIYKALNANVPVFAHVPMILGPDGAKLSKR
HGAVNVMQYREDGYLPQAILNYLVRLGWSHGDQEIFSIEEMIKAFNLEHINASPSRFDFEKLKWLNKHYIKESKFDDIQT
EVEYHFAKIGLDISNGPDLKELVAVMAEKVDTLVELAEKSSYFYSDDISYDENAVKKHIKASTGEIFVKLLENFEALDAQ
QWQDPDVLYNIVSTTAEQCQVGMGKVGMPLRVAITSSGQSPDIGITLKLLGKNKVVARLTKALEELCK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=326, Percent_Identity=35.8895705521472, Blast_Score=215, Evalue=8e-56,
Organism=Homo sapiens, GI4826960, Length=293, Percent_Identity=27.3037542662116, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI62241042, Length=228, Percent_Identity=26.7543859649123, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788743, Length=463, Percent_Identity=56.3714902807775, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI226510925, Length=282, Percent_Identity=34.7517730496454, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1786895, Length=107, Percent_Identity=31.7757009345794, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=487, Percent_Identity=31.4168377823409, Blast_Score=207, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6324540, Length=501, Percent_Identity=30.3393213572854, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6321192, Length=212, Percent_Identity=30.188679245283, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324742, Length=209, Percent_Identity=28.7081339712919, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24665321, Length=493, Percent_Identity=31.8458417849899, Blast_Score=239, Evalue=3e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 53008; Mature: 53008

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITTRFAPSPTGFLHVGGVRTALFSWLYAKNNNGKFILRIEDTDLERSTQEAVDAILDGM
CCCCCCCCCCCCEEEECHHHHHHHHHHHEECCCCEEEEEEECCCCCHHHHHHHHHHHCCH
SWLGLKNDGEIYYQTKRFDRYKEVIQELIADGKAYYCSCSKERLEELREYQQANNLKTGY
HHCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCCC
DGKCRDANYIPQQGESYVVRFKNPQDGVVSWDDAVKGRISISNHELDDMIIQRADGSPTY
CCCCCCCCCCCCCCCEEEEEECCCCCCCEECCCCCCCEEEECCCCHHHHHHCCCCCCCCE
NFCVVVDDIDMAITHIIRGDDHVNNTPKQINIYKALNANVPVFAHVPMILGPDGAKLSKR
EEEEEEECHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCEEEECCEEECCCCCHHHHH
HGAVNVMQYREDGYLPQAILNYLVRLGWSHGDQEIFSIEEMIKAFNLEHINASPSRFDFE
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHH
KLKWLNKHYIKESKFDDIQTEVEYHFAKIGLDISNGPDLKELVAVMAEKVDTLVELAEKS
HHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHC
SYFYSDDISYDENAVKKHIKASTGEIFVKLLENFEALDAQQWQDPDVLYNIVSTTAEQCQ
CCEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHH
VGMGKVGMPLRVAITSSGQSPDIGITLKLLGKNKVVARLTKALEELCK
CCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MITTRFAPSPTGFLHVGGVRTALFSWLYAKNNNGKFILRIEDTDLERSTQEAVDAILDGM
CCCCCCCCCCCCEEEECHHHHHHHHHHHEECCCCEEEEEEECCCCCHHHHHHHHHHHCCH
SWLGLKNDGEIYYQTKRFDRYKEVIQELIADGKAYYCSCSKERLEELREYQQANNLKTGY
HHCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCCC
DGKCRDANYIPQQGESYVVRFKNPQDGVVSWDDAVKGRISISNHELDDMIIQRADGSPTY
CCCCCCCCCCCCCCCEEEEEECCCCCCCEECCCCCCCEEEECCCCHHHHHHCCCCCCCCE
NFCVVVDDIDMAITHIIRGDDHVNNTPKQINIYKALNANVPVFAHVPMILGPDGAKLSKR
EEEEEEECHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCEEEECCEEECCCCCHHHHH
HGAVNVMQYREDGYLPQAILNYLVRLGWSHGDQEIFSIEEMIKAFNLEHINASPSRFDFE
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHH
KLKWLNKHYIKESKFDDIQTEVEYHFAKIGLDISNGPDLKELVAVMAEKVDTLVELAEKS
HHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHC
SYFYSDDISYDENAVKKHIKASTGEIFVKLLENFEALDAQQWQDPDVLYNIVSTTAEQCQ
CCEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHH
VGMGKVGMPLRVAITSSGQSPDIGITLKLLGKNKVVARLTKALEELCK
CCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA