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The map label for this gene is nfnB [H]

Identifier: 89255649

GI number: 89255649

Start: 215352

End: 216035

Strand: Reverse

Name: nfnB [H]

Synonym: FTL_0215

Alternate gene names: 89255649

Gene position: 216035-215352 (Counterclockwise)

Preceding gene: 89255654

Following gene: 89255648

Centisome position: 11.39

GC content: 32.31

Gene sequence:

>684_bases
ATGAATATAGTTGACTACGCTAAAAGCCGTTATACAACAAAAGCTTATGATCCAACAAAAAAACTGTCAAATGAACAAAT
ACAGCAAATAAAAGATATCCTTAGATTTACACCATCAAGTGTAAACTCTCAACCTTGGCACTTTATCTTAGCAACTTCTG
ACCAAGCTAAAGCTAAAATTGCTAAAGCAGCTGAAAATATTCATCCCAAAAATGTAACAAACATTAAAGATTGTGCTTTA
GTCATCGTATTTTGCCTAAAAACTGATATTGATGATGAATATCTAGAGCTACTTCTAAATCAAGAGCGTAAAGATGGTAG
ATTCCCTACTTCAGAATTTGAAAATATGCAAAGAGATGTGCGTAGAAAATTTGCTCTAGCATACAAAGATAAACCCCAAG
AGTTACAAATTTGGACAGAAAAACAACTTTATATTGCTCTTGGTAATATTCTATTAGGACTATCGGCCTTAGAGATAAAT
GCTACACCTATGGAAAGTATAGAGCCACATATAATAGATACTGAATTTAATTTAGCAGAAAAGGGACTTAAAACCTCCGT
AATTGTCACTGCTGGCTATAGCTCTGAAGATGATTTTAATGCTAAGCTACCTAAATCAAGGAGGAAAAAATCTTTACTGA
AGTTTAGTTATTATATCTATCGGAAATATCTCAATGAAATCTAA

Upstream 100 bases:

>100_bases
ATAATTACAAAAAAATAAAAATATCTCTAGCCTAAAAAACATTGTTGAAAAATTTTTTATAAAATATACTGATTGAAACC
TTTAAGATTTTATTTTTATT

Downstream 100 bases:

>100_bases
TTTAACTAAAATCACATCGCTTGATGATATGCGTAAAGTTTATCATCGTAGAGTTCCTAAAATGTTCGTAGACTATTGTG
AAGCTGGTTCATGGCAACAG

Product: dihydropteridine reductase

Products: 6,7-dihydropteridine; NADH; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MNIVDYAKSRYTTKAYDPTKKLSNEQIQQIKDILRFTPSSVNSQPWHFILATSDQAKAKIAKAAENIHPKNVTNIKDCAL
VIVFCLKTDIDDEYLELLLNQERKDGRFPTSEFENMQRDVRRKFALAYKDKPQELQIWTEKQLYIALGNILLGLSALEIN
ATPMESIEPHIIDTEFNLAEKGLKTSVIVTAGYSSEDDFNAKLPKSRRKKSLLKFSYYIYRKYLNEI

Sequences:

>Translated_227_residues
MNIVDYAKSRYTTKAYDPTKKLSNEQIQQIKDILRFTPSSVNSQPWHFILATSDQAKAKIAKAAENIHPKNVTNIKDCAL
VIVFCLKTDIDDEYLELLLNQERKDGRFPTSEFENMQRDVRRKFALAYKDKPQELQIWTEKQLYIALGNILLGLSALEIN
ATPMESIEPHIIDTEFNLAEKGLKTSVIVTAGYSSEDDFNAKLPKSRRKKSLLKFSYYIYRKYLNEI
>Mature_227_residues
MNIVDYAKSRYTTKAYDPTKKLSNEQIQQIKDILRFTPSSVNSQPWHFILATSDQAKAKIAKAAENIHPKNVTNIKDCAL
VIVFCLKTDIDDEYLELLLNQERKDGRFPTSEFENMQRDVRRKFALAYKDKPQELQIWTEKQLYIALGNILLGLSALEIN
ATPMESIEPHIIDTEFNLAEKGLKTSVIVTAGYSSEDDFNAKLPKSRRKKSLLKFSYYIYRKYLNEI

Specific function: Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=207, Percent_Identity=44.4444444444444, Blast_Score=195, Evalue=2e-51,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.5.1.34

Molecular weight: Translated: 26248; Mature: 26248

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVDYAKSRYTTKAYDPTKKLSNEQIQQIKDILRFTPSSVNSQPWHFILATSDQAKAKI
CCCHHHHHHCCCCCCCCCHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHH
AKAAENIHPKNVTNIKDCALVIVFCLKTDIDDEYLELLLNQERKDGRFPTSEFENMQRDV
HHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
RRKFALAYKDKPQELQIWTEKQLYIALGNILLGLSALEINATPMESIEPHIIDTEFNLAE
HHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHEEECCCCHHCCCCCEECCCCCHHH
KGLKTSVIVTAGYSSEDDFNAKLPKSRRKKSLLKFSYYIYRKYLNEI
CCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIVDYAKSRYTTKAYDPTKKLSNEQIQQIKDILRFTPSSVNSQPWHFILATSDQAKAKI
CCCHHHHHHCCCCCCCCCHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHH
AKAAENIHPKNVTNIKDCALVIVFCLKTDIDDEYLELLLNQERKDGRFPTSEFENMQRDV
HHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
RRKFALAYKDKPQELQIWTEKQLYIALGNILLGLSALEINATPMESIEPHIIDTEFNLAE
HHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHEEECCCCHHCCCCCEECCCCCHHH
KGLKTSVIVTAGYSSEDDFNAKLPKSRRKKSLLKFSYYIYRKYLNEI
CCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,6,7,8-tetrahydropteridine; NAD+; NADP+

Specific reaction: a 5,6,7,8-tetrahydropteridine + NAD(P)+ = a 6,7-dihydropteridine + NAD(P)H + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2179862; 11677609 [H]