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The map label for this gene is lpxD1 [H]

Identifier: 89255630

GI number: 89255630

Start: 197393

End: 198436

Strand: Reverse

Name: lpxD1 [H]

Synonym: FTL_0196

Alternate gene names: 89255630

Gene position: 198436-197393 (Counterclockwise)

Preceding gene: 89255631

Following gene: 89255624

Centisome position: 10.47

GC content: 35.63

Gene sequence:

>1044_bases
TTGCAATACACTCTAAAACAAATATCCGAGCATCTAAATGCAAAAGTAATTAATGAACCAAGTGGTGAAGTTATTATAAC
GGGGCTTAACTACGCTGAACAAGCAAAAGAAAATGATCTAACTCTTATTGATAAACAAGAACATATTAAGCTTTGGCAAA
ATTCAAAAGCTGCAGCTGCTATTGTAAGTAAAAAAATTAGTAAAGAATTAGCACAGGTAAATGACAAGCCTCTAATTGTA
GTCAATAATGCTGATTTAGCTATGGCTAAAATATTAGAACTTTTTTCTGTGCCATACCCAGAACAAAATGGTATTCATGA
AAAAGCTGTAATAGATCCAACTGCTAAAATTGGTAAAAATGTCTCAATTGGACCCGGCGCATATATCGGTAAAAATGTCG
AGATTGGTGATAATACGATTATCTATGCCAACGTATGTATCTATAACGATGCAAAAGTTGGAACTAACTGTATTATCTGG
CCAAGTGTGACAATTCGTGATAGAACTATTATAGATCATTTTTGTAGGTTATACTCAAACTGTTCAATCGGATCAGATGG
TTTTGGCTATAGACCGTCTGAAGATGGTAGAACAATTGTTAGGATTCCACACATTGGTAATGTTGTTATCGGTAGCTTTG
TCGATATTGGTTCAAATACTTGTATTAATAATGCTAAATATGGCTCAACTATAATTGGCGATTATACCAAAATTGATAAT
CTTGTTCAGATAGGTCACAATGTAATTATTGGTAAAGGCTGCATGATTTGTGGACAAGCTGGTATCAGTGGTTCAGTAAC
AATTGGTGACGGCGTTATAATTGCTGGTAATGCTGGTATCAAAGATCATACAAATATTGGTAGTGATGCACGTATCGGTG
GTAAAGCTGGAGTTATGTGGGATGTACCTGCTGGTGAAAGCCATATGGGCTATCCCGCTTACAAAGACAGTGAGCTTGCC
AAACAATGGATTGCTATCAGGAAGCTCCCTGAGACTATGAAAAAACTTAAAGCTATTGCTAAAAGCCTCAACATAGATCT
TTAA

Upstream 100 bases:

>100_bases
ATACTGACTTTGGTGTTGGTGACTAAAGCTTAAAGCTTACTATCAAAGTATAGCTTAATCTGCTATGATTATTTAATCAC
AATAAATTTGGAGAAAATGT

Downstream 100 bases:

>100_bases
TTATTTTCTATCTAAAACCATATCTAAATCAACATTGTTAAATTCTCAAGATATGGTCTAATCAATTCTATCTTTTAAGG
GCTGTTGATTTAAAGCTAAG

Product: UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Products: UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine; [Acyl-Carrier Protein]. [C]

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MQYTLKQISEHLNAKVINEPSGEVIITGLNYAEQAKENDLTLIDKQEHIKLWQNSKAAAAIVSKKISKELAQVNDKPLIV
VNNADLAMAKILELFSVPYPEQNGIHEKAVIDPTAKIGKNVSIGPGAYIGKNVEIGDNTIIYANVCIYNDAKVGTNCIIW
PSVTIRDRTIIDHFCRLYSNCSIGSDGFGYRPSEDGRTIVRIPHIGNVVIGSFVDIGSNTCINNAKYGSTIIGDYTKIDN
LVQIGHNVIIGKGCMICGQAGISGSVTIGDGVIIAGNAGIKDHTNIGSDARIGGKAGVMWDVPAGESHMGYPAYKDSELA
KQWIAIRKLPETMKKLKAIAKSLNIDL

Sequences:

>Translated_347_residues
MQYTLKQISEHLNAKVINEPSGEVIITGLNYAEQAKENDLTLIDKQEHIKLWQNSKAAAAIVSKKISKELAQVNDKPLIV
VNNADLAMAKILELFSVPYPEQNGIHEKAVIDPTAKIGKNVSIGPGAYIGKNVEIGDNTIIYANVCIYNDAKVGTNCIIW
PSVTIRDRTIIDHFCRLYSNCSIGSDGFGYRPSEDGRTIVRIPHIGNVVIGSFVDIGSNTCINNAKYGSTIIGDYTKIDN
LVQIGHNVIIGKGCMICGQAGISGSVTIGDGVIIAGNAGIKDHTNIGSDARIGGKAGVMWDVPAGESHMGYPAYKDSELA
KQWIAIRKLPETMKKLKAIAKSLNIDL
>Mature_347_residues
MQYTLKQISEHLNAKVINEPSGEVIITGLNYAEQAKENDLTLIDKQEHIKLWQNSKAAAAIVSKKISKELAQVNDKPLIV
VNNADLAMAKILELFSVPYPEQNGIHEKAVIDPTAKIGKNVSIGPGAYIGKNVEIGDNTIIYANVCIYNDAKVGTNCIIW
PSVTIRDRTIIDHFCRLYSNCSIGSDGFGYRPSEDGRTIVRIPHIGNVVIGSFVDIGSNTCINNAKYGSTIIGDYTKIDN
LVQIGHNVIIGKGCMICGQAGISGSVTIGDGVIIAGNAGIKDHTNIGSDARIGGKAGVMWDVPAGESHMGYPAYKDSELA
KQWIAIRKLPETMKKLKAIAKSLNIDL

Specific function: Lipid A biosynthesis; third step. [C]

COG id: COG1044

COG function: function code M; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family. LpxD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786376, Length=345, Percent_Identity=33.0434782608696, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1786378, Length=206, Percent_Identity=26.2135922330097, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004
- InterPro:   IPR007691
- InterPro:   IPR020573 [H]

Pfam domain/function: PF00132 Hexapep; PF04613 LpxD [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 37429; Mature: 37429

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYTLKQISEHLNAKVINEPSGEVIITGLNYAEQAKENDLTLIDKQEHIKLWQNSKAAAA
CCCHHHHHHHHCCEEEEECCCCCEEEEECCHHHHHCCCCCEEEECHHHHHHHCCCCHHHH
IVSKKISKELAQVNDKPLIVVNNADLAMAKILELFSVPYPEQNGIHEKAVIDPTAKIGKN
HHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHCCC
VSIGPGAYIGKNVEIGDNTIIYANVCIYNDAKVGTNCIIWPSVTIRDRTIIDHFCRLYSN
CCCCCCCEECCEEEECCCEEEEEEEEEEECCCCCCEEEEECCEEECCHHHHHHHHHHHCC
CSIGSDGFGYRPSEDGRTIVRIPHIGNVVIGSFVDIGSNTCINNAKYGSTIIGDYTKIDN
CCCCCCCCCCCCCCCCCEEEEECCCCCEEEHHHEECCCCCCCCCCCCCCEEECCHHHHHH
LVQIGHNVIIGKGCMICGQAGISGSVTIGDGVIIAGNAGIKDHTNIGSDARIGGKAGVMW
HHHHCCEEEEECCEEEEECCCCCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCEEE
DVPAGESHMGYPAYKDSELAKQWIAIRKLPETMKKLKAIAKSLNIDL
ECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQYTLKQISEHLNAKVINEPSGEVIITGLNYAEQAKENDLTLIDKQEHIKLWQNSKAAAA
CCCHHHHHHHHCCEEEEECCCCCEEEEECCHHHHHCCCCCEEEECHHHHHHHCCCCHHHH
IVSKKISKELAQVNDKPLIVVNNADLAMAKILELFSVPYPEQNGIHEKAVIDPTAKIGKN
HHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHCCC
VSIGPGAYIGKNVEIGDNTIIYANVCIYNDAKVGTNCIIWPSVTIRDRTIIDHFCRLYSN
CCCCCCCEECCEEEECCCEEEEEEEEEEECCCCCCEEEEECCEEECCHHHHHHHHHHHCC
CSIGSDGFGYRPSEDGRTIVRIPHIGNVVIGSFVDIGSNTCINNAKYGSTIIGDYTKIDN
CCCCCCCCCCCCCCCCCEEEEECCCCCEEEHHHEECCCCCCCCCCCCCCEEECCHHHHHH
LVQIGHNVIIGKGCMICGQAGISGSVTIGDGVIIAGNAGIKDHTNIGSDARIGGKAGVMW
HHHHCCEEEEECCEEEEECCCCCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCEEE
DVPAGESHMGYPAYKDSELAKQWIAIRKLPETMKKLKAIAKSLNIDL
ECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine; (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] [C]

Specific reaction: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine + (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] = UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine + [Acyl-Carrier Protein]. [C]

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA