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The map label for this gene is cyoE [H]

Identifier: 89255629

GI number: 89255629

Start: 196400

End: 197248

Strand: Direct

Name: cyoE [H]

Synonym: FTL_0195

Alternate gene names: 89255629

Gene position: 196400-197248 (Clockwise)

Preceding gene: 89255628

Following gene: 89255634

Centisome position: 10.36

GC content: 30.74

Gene sequence:

>849_bases
ATGTATTTCAAAAGATATCTTCAATTAGCTAAGCCAGGTATTATTTTTGGTAATCTTATTACTCTTACAGGTGGTTTTTT
ATTAGCTACACATCGAGAAATAGGTTTTGAATATCTACCTTTGTTTGTATATGTGATGATTGGCGTTGCATTAATGATTG
CTGCTGGTTGTGTCTTTAACAATATTTATGATCAAGATATTGATAGTAGTATGACACGTACGCAAAATCGTCCTTTAGTT
ACTGGAGATATCTCTGTAATCCAAGCAACTATATATGGGACGATATTACTTATATTAAGTTGTTTGGTTTTATATTATTT
AGTCAATCTATTGACACTATGGATTATAATTATAGGTTTTATAGTCTACGTAGGTATTTATACAGTTTCAAAACGTTTGA
CGATACATGCGACAGTTTTAGGCGGTATTTCAGGAGCAATCCCTCCAGTAGCTGGCTATACGGCTGTAGTTAACATATTA
GATTATAATGCTTTAGCTTTGTTTTTAATTCTATTTTTTTGGCAAATTCCTCATTCGTATGCTATAGCGATGTTATATAT
CGATGATTATAAAAAAGTTAAATTACCAATGCTTCCAATAGTCAAAGGTATAGCATACACTAAAAAAATTATGCTATTTT
ATCTAGCTTTATTTGTGGTTTCATGTGCTTTACCAGCAGTACTTGGAAGTGCAGATCTATTTTCATTTATTGTATGTATG
CTTGTAGCACTATTTTGGATGTATAAATCTATACAGTCTTATAGAACCGATACAGATAGAGTTTTTGCTAAAACTGTATT
TAAATTCTCAATAATTGTTATTACAGCTATTTGTCTGACAATGGGTTAG

Upstream 100 bases:

>100_bases
TGTTGTAGTTGTAATTGTAGTTGCTGGTACTATGTGGATCATGTTTGACTTATATGACATGATGATGTAATTCTCTTTTT
TAAAAAATATTTTTCTTACT

Downstream 100 bases:

>100_bases
TTGTTTTCATTCGATAATAAAAACAAAACTATATGTGCTAATGACTTAGCTTTAAATCAACAGCCCTTAAAAGATAGAAT
TGATTAGACCATATCTTGAG

Product: protoheme IX farnesyltransferase

Products: heme O; pyrophosphate [C]

Alternate protein names: Heme B farnesyltransferase; Heme O synthase [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MYFKRYLQLAKPGIIFGNLITLTGGFLLATHREIGFEYLPLFVYVMIGVALMIAAGCVFNNIYDQDIDSSMTRTQNRPLV
TGDISVIQATIYGTILLILSCLVLYYLVNLLTLWIIIIGFIVYVGIYTVSKRLTIHATVLGGISGAIPPVAGYTAVVNIL
DYNALALFLILFFWQIPHSYAIAMLYIDDYKKVKLPMLPIVKGIAYTKKIMLFYLALFVVSCALPAVLGSADLFSFIVCM
LVALFWMYKSIQSYRTDTDRVFAKTVFKFSIIVITAICLTMG

Sequences:

>Translated_282_residues
MYFKRYLQLAKPGIIFGNLITLTGGFLLATHREIGFEYLPLFVYVMIGVALMIAAGCVFNNIYDQDIDSSMTRTQNRPLV
TGDISVIQATIYGTILLILSCLVLYYLVNLLTLWIIIIGFIVYVGIYTVSKRLTIHATVLGGISGAIPPVAGYTAVVNIL
DYNALALFLILFFWQIPHSYAIAMLYIDDYKKVKLPMLPIVKGIAYTKKIMLFYLALFVVSCALPAVLGSADLFSFIVCM
LVALFWMYKSIQSYRTDTDRVFAKTVFKFSIIVITAICLTMG
>Mature_282_residues
MYFKRYLQLAKPGIIFGNLITLTGGFLLATHREIGFEYLPLFVYVMIGVALMIAAGCVFNNIYDQDIDSSMTRTQNRPLV
TGDISVIQATIYGTILLILSCLVLYYLVNLLTLWIIIIGFIVYVGIYTVSKRLTIHATVLGGISGAIPPVAGYTAVVNIL
DYNALALFLILFFWQIPHSYAIAMLYIDDYKKVKLPMLPIVKGIAYTKKIMLFYLALFVVSCALPAVLGSADLFSFIVCM
LVALFWMYKSIQSYRTDTDRVFAKTVFKFSIIVITAICLTMG

Specific function: Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group [H]

COG id: COG0109

COG function: function code O; Polyprenyltransferase (cytochrome oxidase assembly factor)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily [H]

Homologues:

Organism=Homo sapiens, GI17921982, Length=172, Percent_Identity=36.046511627907, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI1786631, Length=282, Percent_Identity=43.9716312056738, Blast_Score=246, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI71998235, Length=147, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325085, Length=188, Percent_Identity=28.1914893617021, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19921064, Length=261, Percent_Identity=28.3524904214559, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006369
- InterPro:   IPR000537 [H]

Pfam domain/function: PF01040 UbiA [H]

EC number: 2.5.1.- [C]

Molecular weight: Translated: 31544; Mature: 31544

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS00943 UBIA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFKRYLQLAKPGIIFGNLITLTGGFLLATHREIGFEYLPLFVYVMIGVALMIAAGCVFN
CCHHHHHHHHCCCHHHHHHHHHHCCHHEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
NIYDQDIDSSMTRTQNRPLVTGDISVIQATIYGTILLILSCLVLYYLVNLLTLWIIIIGF
HHHHHCCCHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVYVGIYTVSKRLTIHATVLGGISGAIPPVAGYTAVVNILDYNALALFLILFFWQIPHSY
HHHHHHHHHHHHHEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
AIAMLYIDDYKKVKLPMLPIVKGIAYTKKIMLFYLALFVVSCALPAVLGSADLFSFIVCM
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LVALFWMYKSIQSYRTDTDRVFAKTVFKFSIIVITAICLTMG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYFKRYLQLAKPGIIFGNLITLTGGFLLATHREIGFEYLPLFVYVMIGVALMIAAGCVFN
CCHHHHHHHHCCCHHHHHHHHHHCCHHEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
NIYDQDIDSSMTRTQNRPLVTGDISVIQATIYGTILLILSCLVLYYLVNLLTLWIIIIGF
HHHHHCCCHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVYVGIYTVSKRLTIHATVLGGISGAIPPVAGYTAVVNILDYNALALFLILFFWQIPHSY
HHHHHHHHHHHHHEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
AIAMLYIDDYKKVKLPMLPIVKGIAYTKKIMLFYLALFVVSCALPAVLGSADLFSFIVCM
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LVALFWMYKSIQSYRTDTDRVFAKTVFKFSIIVITAICLTMG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protoheme; farnesyl-PP [C]

Specific reaction: protoheme + farnesyl-PP = heme O + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA