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The map label for this gene is leuA1 [H]

Identifier: 89255566

GI number: 89255566

Start: 131814

End: 132632

Strand: Reverse

Name: leuA1 [H]

Synonym: FTL_0129

Alternate gene names: 89255566

Gene position: 132632-131814 (Counterclockwise)

Preceding gene: 89255567

Following gene: 89255563

Centisome position: 7.0

GC content: 35.78

Gene sequence:

>819_bases
ATGGATGCCTTACGCCCATCATTAGCAATAGGTAAAGCTAGAGTGCATATGTATTTACCAGTAGATCCAAATTTAGCTCA
AGCAAGTCTAGGTAGTAAAAATGATAATGAGCAGAATATTAAAAATGTTTATGAATTAGTTAAACTAGCTAGTGATGAGG
GCTTTGAGGTTGAGTTTAGTGCCGAAGGATATTCAAGGCTAGGCGATACTTTTGATTATGTTACTAATATTTTTAGAGCT
GCTGTAGCTGCTGGGGTTAAAGTTATTAACTGTCCTGACACTATTGGCGGAGCATGCGAGCGTGAAAATGATAATTATTT
TGTCAAAAATATGGCTAAACATGCTGAAATCATCAAAAAAGAATTCCCTGATAGAAATATAATCTGGTCAGCACATTGTC
ATAATGATTTGGGATTAGCTTTAGAAAACTCTATGAATGCTGTTTTTGATGGTCCAGCACGACAAGTAGAGGGCTGTATC
AATGGAGTTGGTGAGAGAGCCGGTAATGCCCCTTTAGAGCAATGTGTGATGTTTATAAATCTTTTTGGTAAGCAAAAAGA
CTATCATTACTACAATGATGTTAATATTGCACATTTTAAAACTATTTCTGATTTTATCGGCAAAAGAATGTTATCAAGAC
AGCCACATCACCCTATTACTGGTCTAAACTCAGCAAGGCATACTTCTGGTGGACATACAAATGCCATACTAAAAAATCCT
ATTGCATATCAGCCATTCCATCCAGAATCTGTCGGTAATGAAATTAGCTTTATTTTTGGTCCATTATCAGGTGGTAACCA
TGCTAAGAAAATTATCTAA

Upstream 100 bases:

>100_bases
GACTTTGAAATAATAAATACTATCTCAAAGAGGATGGCTGAGAGAAACTCTAATATGATTAGGACTATGTCAGTTAAGAA
AAAACCAAGTTGAAATAACT

Downstream 100 bases:

>100_bases
GAAAATGGTTATATTTGTGATGACAAAGAAAAGGCTGCTATAGCTCAGCAAATAAAAGATATTTATCATGAGCGTAGAAA
AGGTATCACAGATGAAGAGC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1 [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MDALRPSLAIGKARVHMYLPVDPNLAQASLGSKNDNEQNIKNVYELVKLASDEGFEVEFSAEGYSRLGDTFDYVTNIFRA
AVAAGVKVINCPDTIGGACERENDNYFVKNMAKHAEIIKKEFPDRNIIWSAHCHNDLGLALENSMNAVFDGPARQVEGCI
NGVGERAGNAPLEQCVMFINLFGKQKDYHYYNDVNIAHFKTISDFIGKRMLSRQPHHPITGLNSARHTSGGHTNAILKNP
IAYQPFHPESVGNEISFIFGPLSGGNHAKKII

Sequences:

>Translated_272_residues
MDALRPSLAIGKARVHMYLPVDPNLAQASLGSKNDNEQNIKNVYELVKLASDEGFEVEFSAEGYSRLGDTFDYVTNIFRA
AVAAGVKVINCPDTIGGACERENDNYFVKNMAKHAEIIKKEFPDRNIIWSAHCHNDLGLALENSMNAVFDGPARQVEGCI
NGVGERAGNAPLEQCVMFINLFGKQKDYHYYNDVNIAHFKTISDFIGKRMLSRQPHHPITGLNSARHTSGGHTNAILKNP
IAYQPFHPESVGNEISFIFGPLSGGNHAKKII
>Mature_272_residues
MDALRPSLAIGKARVHMYLPVDPNLAQASLGSKNDNEQNIKNVYELVKLASDEGFEVEFSAEGYSRLGDTFDYVTNIFRA
AVAAGVKVINCPDTIGGACERENDNYFVKNMAKHAEIIKKEFPDRNIIWSAHCHNDLGLALENSMNAVFDGPARQVEGCI
NGVGERAGNAPLEQCVMFINLFGKQKDYHYYNDVNIAHFKTISDFIGKRMLSRQPHHPITGLNSARHTSGGHTNAILKNP
IAYQPFHPESVGNEISFIFGPLSGGNHAKKII

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=271, Percent_Identity=32.8413284132841, Blast_Score=105, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6320071, Length=271, Percent_Identity=26.9372693726937, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320019, Length=271, Percent_Identity=26.5682656826568, Blast_Score=73, Evalue=6e-14,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 30015; Mature: 30015

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALRPSLAIGKARVHMYLPVDPNLAQASLGSKNDNEQNIKNVYELVKLASDEGFEVEFS
CCCCCCCCEECEEEEEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEC
AEGYSRLGDTFDYVTNIFRAAVAAGVKVINCPDTIGGACERENDNYFVKNMAKHAEIIKK
CCCHHHHCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHH
EFPDRNIIWSAHCHNDLGLALENSMNAVFDGPARQVEGCINGVGERAGNAPLEQCVMFIN
HCCCCCCEEEECCCCCCCEEHHCCCCHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LFGKQKDYHYYNDVNIAHFKTISDFIGKRMLSRQPHHPITGLNSARHTSGGHTNAILKNP
HHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCC
IAYQPFHPESVGNEISFIFGPLSGGNHAKKII
CCCCCCCCHHHCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MDALRPSLAIGKARVHMYLPVDPNLAQASLGSKNDNEQNIKNVYELVKLASDEGFEVEFS
CCCCCCCCEECEEEEEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEC
AEGYSRLGDTFDYVTNIFRAAVAAGVKVINCPDTIGGACERENDNYFVKNMAKHAEIIKK
CCCHHHHCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHH
EFPDRNIIWSAHCHNDLGLALENSMNAVFDGPARQVEGCINGVGERAGNAPLEQCVMFIN
HCCCCCCEEEECCCCCCCEEHHCCCCHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LFGKQKDYHYYNDVNIAHFKTISDFIGKRMLSRQPHHPITGLNSARHTSGGHTNAILKNP
HHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCC
IAYQPFHPESVGNEISFIFGPLSGGNHAKKII
CCCCCCCCHHHCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]