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The map label for this gene is purT [H]

Identifier: 89255532

GI number: 89255532

Start: 85682

End: 86842

Strand: Reverse

Name: purT [H]

Synonym: FTL_0092

Alternate gene names: 89255532

Gene position: 86842-85682 (Counterclockwise)

Preceding gene: 89255533

Following gene: 89255529

Centisome position: 4.58

GC content: 35.49

Gene sequence:

>1161_bases
ATGAATATTTCTAATATCAAAATTATGTTGCTTGGTTCAGGTGAACTAGGCAAAGAATTCATAATAGCTGCTCAAAGACT
TGGTATACACACTATAGCTGTAGATCGATATAAAAATGCACCAGCTATGCAAGTAGCTCACGAAAGCTATGTTATAGATA
TGCTAAATTCAGATGCTCTTGAGCAACTAATTCTAGCAAAAAATCCAACGTATATAGTTCCTGAAATTGAAGCTATAAAT
ACCGATAGCTTAGTAAAACTAGAAGCTCATAACTTCAATATTATCCCATGTGCAAAAGCCACGAAACTAACTATGGATCG
CCAGGGTATAAGAGCTTTAGCTGTACAACAGCTTAATTTACAAACATCAAAATTTGCATTTGCAAATAGCGAGCAGGAAT
ATCTAGATGTAATACAATCGATTGGTCTACCATTTGTGATTAAGCCTGTAATGAGCTCTTCAGGTAAAGGTCAATCAATT
GTTAAAGAACATAATGAAATTAAAAAAGCTTGGGATTATGCTCAAAATGGCTCTCGAGGCCATGCTAAAGGTGTAATTGT
AGAACAATTTATAGATTTTGACTATGAGATAACGCTACTAACTGTTAGACATAAAGATGGTACTTCATTTTGCGACCCTA
TTGGACATATCCAAAAAGATGGTGATTATCGCTTCTCTTGGCAACCTCATACAATGCCAGATACGGCTTTAGCAAAGTCA
CAAGAAATTGCTAAAGAAATCACCGATGCATTAGGTGGCTATGGAGTTTTTGGAGTTGAGTTATTCATAAAAGGTGATGA
GGTATTTTTTAATGAAGTCTCACCTCGTCCTCATGACACGGGGATGGTGACACTTATATCTCAAAATATAAACGAATTTG
AGCTACATCTTCGTGCAATAGTTGGACTACCTATACCTGATATACAGACATTACAGCCATCTGCTTCTGCAGCAATATTA
CTTGAAGGAGATACTGCTAACGCAAGTATTTGCGGTATTGATAAAGCATTAGCTGATGCAAATGTAGATATAAGAATATT
TAGCAAAAAAGAAATACATGGTAAACGCCGTATGGGAGTTGTCTTAGCAAAAGCACAAAATACTCACATAGCTTTAGAAA
CTTCTAAGCAAGCTCTAGCACATATTCATCTTACTAAATAA

Upstream 100 bases:

>100_bases
ACTTTGGTAAACAAATTACAGAGTTTTATCCTATAAATGATAAATAAACCGACATTTTTCTGCTAACATATTTCTTAACT
TTAAAATTTTCTTTTAGCCA

Downstream 100 bases:

>100_bases
GCTAACGCTTTCTAAGTAAATTTATACCATCTCCAATCGGTATTATACAAGCGTCTACGCGCTTATCATCATGAATTAAG
TTATTTAGCTGTCTTATTAC

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MNISNIKIMLLGSGELGKEFIIAAQRLGIHTIAVDRYKNAPAMQVAHESYVIDMLNSDALEQLILAKNPTYIVPEIEAIN
TDSLVKLEAHNFNIIPCAKATKLTMDRQGIRALAVQQLNLQTSKFAFANSEQEYLDVIQSIGLPFVIKPVMSSSGKGQSI
VKEHNEIKKAWDYAQNGSRGHAKGVIVEQFIDFDYEITLLTVRHKDGTSFCDPIGHIQKDGDYRFSWQPHTMPDTALAKS
QEIAKEITDALGGYGVFGVELFIKGDEVFFNEVSPRPHDTGMVTLISQNINEFELHLRAIVGLPIPDIQTLQPSASAAIL
LEGDTANASICGIDKALADANVDIRIFSKKEIHGKRRMGVVLAKAQNTHIALETSKQALAHIHLTK

Sequences:

>Translated_386_residues
MNISNIKIMLLGSGELGKEFIIAAQRLGIHTIAVDRYKNAPAMQVAHESYVIDMLNSDALEQLILAKNPTYIVPEIEAIN
TDSLVKLEAHNFNIIPCAKATKLTMDRQGIRALAVQQLNLQTSKFAFANSEQEYLDVIQSIGLPFVIKPVMSSSGKGQSI
VKEHNEIKKAWDYAQNGSRGHAKGVIVEQFIDFDYEITLLTVRHKDGTSFCDPIGHIQKDGDYRFSWQPHTMPDTALAKS
QEIAKEITDALGGYGVFGVELFIKGDEVFFNEVSPRPHDTGMVTLISQNINEFELHLRAIVGLPIPDIQTLQPSASAAIL
LEGDTANASICGIDKALADANVDIRIFSKKEIHGKRRMGVVLAKAQNTHIALETSKQALAHIHLTK
>Mature_386_residues
MNISNIKIMLLGSGELGKEFIIAAQRLGIHTIAVDRYKNAPAMQVAHESYVIDMLNSDALEQLILAKNPTYIVPEIEAIN
TDSLVKLEAHNFNIIPCAKATKLTMDRQGIRALAVQQLNLQTSKFAFANSEQEYLDVIQSIGLPFVIKPVMSSSGKGQSI
VKEHNEIKKAWDYAQNGSRGHAKGVIVEQFIDFDYEITLLTVRHKDGTSFCDPIGHIQKDGDYRFSWQPHTMPDTALAKS
QEIAKEITDALGGYGVFGVELFIKGDEVFFNEVSPRPHDTGMVTLISQNINEFELHLRAIVGLPIPDIQTLQPSASAAIL
LEGDTANASICGIDKALADANVDIRIFSKKEIHGKRRMGVVLAKAQNTHIALETSKQALAHIHLTK

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=378, Percent_Identity=54.4973544973545, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1786733, Length=313, Percent_Identity=26.8370607028754, Blast_Score=88, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6324702, Length=367, Percent_Identity=27.7929155313352, Blast_Score=135, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42533; Mature: 42533

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISNIKIMLLGSGELGKEFIIAAQRLGIHTIAVDRYKNAPAMQVAHESYVIDMLNSDAL
CCCCEEEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCHHEEHHHHHEEEHHCHHHH
EQLILAKNPTYIVPEIEAINTDSLVKLEAHNFNIIPCAKATKLTMDRQGIRALAVQQLNL
HHHHHCCCCCEEECCEEECCCCCEEEEECCCEEEEECCCCEEEEECCCCHHHHHHHHHCC
QTSKFAFANSEQEYLDVIQSIGLPFVIKPVMSSSGKGQSIVKEHNEIKKAWDYAQNGSRG
CHHEEEECCCCHHHHHHHHHCCCCEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
HAKGVIVEQFIDFDYEITLLTVRHKDGTSFCDPIGHIQKDGDYRFSWQPHTMPDTALAKS
CCCCEEEEHHHCCCEEEEEEEEEECCCCCHHCHHHCCCCCCCEEEEECCCCCCHHHHHHH
QEIAKEITDALGGYGVFGVELFIKGDEVFFNEVSPRPHDTGMVTLISQNINEFELHLRAI
HHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEEECCCCHHHEEEEHE
VGLPIPDIQTLQPSASAAILLEGDTANASICGIDKALADANVDIRIFSKKEIHGKRRMGV
ECCCCCCCCCCCCCCCEEEEEECCCCCCEEECCHHHHHCCCCEEEEEECHHCCCCCCEEE
VLAKAQNTHIALETSKQALAHIHLTK
EEEECCCCEEEEEECCCEEEEEEECC
>Mature Secondary Structure
MNISNIKIMLLGSGELGKEFIIAAQRLGIHTIAVDRYKNAPAMQVAHESYVIDMLNSDAL
CCCCEEEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCHHEEHHHHHEEEHHCHHHH
EQLILAKNPTYIVPEIEAINTDSLVKLEAHNFNIIPCAKATKLTMDRQGIRALAVQQLNL
HHHHHCCCCCEEECCEEECCCCCEEEEECCCEEEEECCCCEEEEECCCCHHHHHHHHHCC
QTSKFAFANSEQEYLDVIQSIGLPFVIKPVMSSSGKGQSIVKEHNEIKKAWDYAQNGSRG
CHHEEEECCCCHHHHHHHHHCCCCEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
HAKGVIVEQFIDFDYEITLLTVRHKDGTSFCDPIGHIQKDGDYRFSWQPHTMPDTALAKS
CCCCEEEEHHHCCCEEEEEEEEEECCCCCHHCHHHCCCCCCCEEEEECCCCCCHHHHHHH
QEIAKEITDALGGYGVFGVELFIKGDEVFFNEVSPRPHDTGMVTLISQNINEFELHLRAI
HHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEEECCCCHHHEEEEHE
VGLPIPDIQTLQPSASAAILLEGDTANASICGIDKALADANVDIRIFSKKEIHGKRRMGV
ECCCCCCCCCCCCCCCEEEEEECCCCCCEEECCHHHHHCCCCEEEEEECHHCCCCCCEEE
VLAKAQNTHIALETSKQALAHIHLTK
EEEECCCCEEEEEECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA