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The map label for this gene is frdA [C]

Identifier: 89056460

GI number: 89056460

Start: 4065615

End: 4067372

Strand: Direct

Name: frdA [C]

Synonym: Jann_3969

Alternate gene names: 89056460

Gene position: 4065615-4067372 (Clockwise)

Preceding gene: 89056459

Following gene: 89056461

Centisome position: 94.16

GC content: 63.03

Gene sequence:

>1758_bases
ATGCGTATCCAAGATATTGAACGTCATGATACCGATATCCTGATCCTTGGCACCGGCGGCGCGGGTCTGTTTGCGGCGTT
GCACGGCCAACAAACAGCGCCAGAGGGGACGAAAATCACTATCGCCGTGAAGGGCCTGATCGGCAAATGCGGCTGCACGC
GGATGGTTCAGGGCGGCTACAACGTGGCCCTTGGCGGTGGTGACACGGTTGAGCGGCACTTCATGGACACGATCAATGGC
GGCAAATGGCTGCCCAATCAGGACATGGCGTGGCGGCTGTGCGAACAGGCCGTGGTGCGCATCCGCGAGTTGGAGAATGA
GATCGGGTGTTTCTTTGATCGCAACGCCGACGGGTCGTTGCACCAGAAGGCGTTTGCCGGTCAGACGGCGGACCGCACGG
TCCACAAGGGCGACCTGACGGGGATCGAGATTATCAATCGCCTGATGGAGAAGGTTCTGGCCAGCGGCGTGGAGAAGCTG
CAGGAGCACCGCGCGGTGGGGCTGATCCCGACCAAAGACGGCAGTGCGTTGGCGGGTGTTTTGATGATCGACATGCGGAC
AGGGGGCTTCAGGCTGGTTCGCGCCAAAACCGTGATGATGGGCACGGGCGGCGGGCCCACGATGTACAAGTACCACACGC
CGTCCGGTGACAAGACAATGGACGGGCTGGCGATGGCTCTGCGCGCAGGACTGCCGTTGCGCGATATGGAGATGGTGCAG
TTTCACCCCACGGGGCTTCTGGCGGGCGATCATACGCGGATGACCGGCACGGTGCTGGAAGAAGGCCTGCGCGGGGCGGG
CGGCCAATTGATCAGCCACGCGGGCAATCGGTTCATGTTCGACTACGACGGCAAAGGCGAGCGTGCGACCCGCGATGTGG
TGTCGCGCGGGATTTATGCGGAGATGCGCAAGAACAACAACCCGGAGCAGGAGGGCGTGTTCATCTCGATGAGCCACCTT
GGGCCGGAGTGGGTAGCGCAGAAATTCAAGGGCATGGTCAAGCGTTGTGCGGACAGCGGGTTCGATCTGGCCGCTGGCAA
GGTAGAGGTGGTGCCGACGGCGCATTACTTCATGGGTGGCGTGGTCGTGGATGTGGATACGCGCGCCACAATGGAAGGTC
TGTATGTGGCGGGCGAAGACGCGGGCGGGGCGCACGGATCGAACCGCCTGGGCGGGAATGGCGTGGCGAATTCAACGGTC
TACGGCGGGATCGCTGGCGACACGATGGGCCGCGACGTGGGCGGTATGTCGTTGCGCGAGCCTGATGAGGATGTGCTGGC
CGCAGAGTTTGAGCGCGCCATCTATCCGCTGTCGAAAAAGCCTGATCTGGTGCTGCCCCTGCGCAAACAATTGCAGGAAT
GCATGTGGGAAGAGGTGGGCGTGATGCGGACGGAGGCGGGGATGCAGCGCGGGCTGGCGGGGATCGCAGAGGTCTCCGAC
GCGCTGATGGATGTGGGCGTGAATGGCAGCAACCTGGCGTTCAACCTGACCTGGCATGATTGGCTGAACATGGCGAGCCT
GTGCGATATCTCGGAGGTGATCACCAGGGCGGGGATCGCGCGCGAAAACTCTCGCGGCGCGCATTTCCGGGAGGATTTTC
CAGAGCCCGGCGCGATGGAGGACAGCGAGTTTACCGTGGCGCGGATGGAGGGTGGCAAGGTCGCCGTCACCCGCGAACCG
GTCCAGTTTACCATCGTGCGGCCCGGCGAAACGGTGCTGCCCGACGACGCGCCCGAGACATTGGTGGCGGCACAATGA

Upstream 100 bases:

>100_bases
TGGAATGGCTACCGTGGAGCGGACCGCAAAAGACGCTCGCCGCAGGCGCCACCGCCGCGTCCTTCTTCATCGCAACCCTT
TTCTTCATGCAGGCCATCTG

Downstream 100 bases:

>100_bases
GGGGTGGATTGCGCCGACTTCGTCGTCGCCCAGCGGGGGCCGGCCCCCGCACCCCCGGGATATTTCTGGAACGGGGAAAA
GGGCGGAGCGCCTCCGCTGC

Product: L-aspartate oxidase

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MRIQDIERHDTDILILGTGGAGLFAALHGQQTAPEGTKITIAVKGLIGKCGCTRMVQGGYNVALGGGDTVERHFMDTING
GKWLPNQDMAWRLCEQAVVRIRELENEIGCFFDRNADGSLHQKAFAGQTADRTVHKGDLTGIEIINRLMEKVLASGVEKL
QEHRAVGLIPTKDGSALAGVLMIDMRTGGFRLVRAKTVMMGTGGGPTMYKYHTPSGDKTMDGLAMALRAGLPLRDMEMVQ
FHPTGLLAGDHTRMTGTVLEEGLRGAGGQLISHAGNRFMFDYDGKGERATRDVVSRGIYAEMRKNNNPEQEGVFISMSHL
GPEWVAQKFKGMVKRCADSGFDLAAGKVEVVPTAHYFMGGVVVDVDTRATMEGLYVAGEDAGGAHGSNRLGGNGVANSTV
YGGIAGDTMGRDVGGMSLREPDEDVLAAEFERAIYPLSKKPDLVLPLRKQLQECMWEEVGVMRTEAGMQRGLAGIAEVSD
ALMDVGVNGSNLAFNLTWHDWLNMASLCDISEVITRAGIARENSRGAHFREDFPEPGAMEDSEFTVARMEGGKVAVTREP
VQFTIVRPGETVLPDDAPETLVAAQ

Sequences:

>Translated_585_residues
MRIQDIERHDTDILILGTGGAGLFAALHGQQTAPEGTKITIAVKGLIGKCGCTRMVQGGYNVALGGGDTVERHFMDTING
GKWLPNQDMAWRLCEQAVVRIRELENEIGCFFDRNADGSLHQKAFAGQTADRTVHKGDLTGIEIINRLMEKVLASGVEKL
QEHRAVGLIPTKDGSALAGVLMIDMRTGGFRLVRAKTVMMGTGGGPTMYKYHTPSGDKTMDGLAMALRAGLPLRDMEMVQ
FHPTGLLAGDHTRMTGTVLEEGLRGAGGQLISHAGNRFMFDYDGKGERATRDVVSRGIYAEMRKNNNPEQEGVFISMSHL
GPEWVAQKFKGMVKRCADSGFDLAAGKVEVVPTAHYFMGGVVVDVDTRATMEGLYVAGEDAGGAHGSNRLGGNGVANSTV
YGGIAGDTMGRDVGGMSLREPDEDVLAAEFERAIYPLSKKPDLVLPLRKQLQECMWEEVGVMRTEAGMQRGLAGIAEVSD
ALMDVGVNGSNLAFNLTWHDWLNMASLCDISEVITRAGIARENSRGAHFREDFPEPGAMEDSEFTVARMEGGKVAVTREP
VQFTIVRPGETVLPDDAPETLVAAQ
>Mature_585_residues
MRIQDIERHDTDILILGTGGAGLFAALHGQQTAPEGTKITIAVKGLIGKCGCTRMVQGGYNVALGGGDTVERHFMDTING
GKWLPNQDMAWRLCEQAVVRIRELENEIGCFFDRNADGSLHQKAFAGQTADRTVHKGDLTGIEIINRLMEKVLASGVEKL
QEHRAVGLIPTKDGSALAGVLMIDMRTGGFRLVRAKTVMMGTGGGPTMYKYHTPSGDKTMDGLAMALRAGLPLRDMEMVQ
FHPTGLLAGDHTRMTGTVLEEGLRGAGGQLISHAGNRFMFDYDGKGERATRDVVSRGIYAEMRKNNNPEQEGVFISMSHL
GPEWVAQKFKGMVKRCADSGFDLAAGKVEVVPTAHYFMGGVVVDVDTRATMEGLYVAGEDAGGAHGSNRLGGNGVANSTV
YGGIAGDTMGRDVGGMSLREPDEDVLAAEFERAIYPLSKKPDLVLPLRKQLQECMWEEVGVMRTEAGMQRGLAGIAEVSD
ALMDVGVNGSNLAFNLTWHDWLNMASLCDISEVITRAGIARENSRGAHFREDFPEPGAMEDSEFTVARMEGGKVAVTREP
VQFTIVRPGETVLPDDAPETLVAAQ

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=524, Percent_Identity=31.1068702290076, Blast_Score=212, Evalue=7e-55,
Organism=Escherichia coli, GI1790597, Length=501, Percent_Identity=30.7385229540918, Blast_Score=222, Evalue=6e-59,
Organism=Escherichia coli, GI1788928, Length=545, Percent_Identity=29.9082568807339, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI1786942, Length=510, Percent_Identity=31.7647058823529, Blast_Score=207, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17550100, Length=528, Percent_Identity=30.4924242424242, Blast_Score=207, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17505833, Length=529, Percent_Identity=31.1909262759924, Blast_Score=202, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6322701, Length=526, Percent_Identity=30.6083650190114, Blast_Score=210, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6322416, Length=535, Percent_Identity=30.4672897196262, Blast_Score=210, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6320788, Length=490, Percent_Identity=26.530612244898, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137288, Length=528, Percent_Identity=31.6287878787879, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24655642, Length=528, Percent_Identity=31.6287878787879, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24655647, Length=528, Percent_Identity=31.6287878787879, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24663005, Length=512, Percent_Identity=30.078125, Blast_Score=194, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1

Molecular weight: Translated: 63059; Mature: 63059

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIQDIERHDTDILILGTGGAGLFAALHGQQTAPEGTKITIAVKGLIGKCGCTRMVQGGY
CCCCCCCCCCCCEEEEECCCCHHHHEECCCCCCCCCCEEEEEEECCCCCCCCHHHHCCCC
NVALGGGDTVERHFMDTINGGKWLPNQDMAWRLCEQAVVRIRELENEIGCFFDRNADGSL
EEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCH
HQKAFAGQTADRTVHKGDLTGIEIINRLMEKVLASGVEKLQEHRAVGLIPTKDGSALAGV
HHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEE
LMIDMRTGGFRLVRAKTVMMGTGGGPTMYKYHTPSGDKTMDGLAMALRAGLPLRDMEMVQ
EEEEECCCCEEEEEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEE
FHPTGLLAGDHTRMTGTVLEEGLRGAGGQLISHAGNRFMFDYDGKGERATRDVVSRGIYA
ECCCEEEECCCCHHHHHHHHHHHCCCCCHHHHHCCCEEEEEECCCCCHHHHHHHHCCHHH
EMRKNNNPEQEGVFISMSHLGPEWVAQKFKGMVKRCADSGFDLAAGKVEVVPTAHYFMGG
HHHCCCCCCCCCEEEEHHHCCHHHHHHHHHHHHHHHHCCCCEEECCEEEEEECHHHHHCC
VVVDVDTRATMEGLYVAGEDAGGAHGSNRLGGNGVANSTVYGGIAGDTMGRDVGGMSLRE
EEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCC
PDEDVLAAEFERAIYPLSKKPDLVLPLRKQLQECMWEEVGVMRTEAGMQRGLAGIAEVSD
CCHHHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
ALMDVGVNGSNLAFNLTWHDWLNMASLCDISEVITRAGIARENSRGAHFREDFPEPGAME
HHHHCCCCCCCEEEEEEHHHHHCHHHHCCHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCC
DSEFTVARMEGGKVAVTREPVQFTIVRPGETVLPDDAPETLVAAQ
CCCEEEEEECCCEEEEEECCEEEEEECCCCCCCCCCCCCCCEECC
>Mature Secondary Structure
MRIQDIERHDTDILILGTGGAGLFAALHGQQTAPEGTKITIAVKGLIGKCGCTRMVQGGY
CCCCCCCCCCCCEEEEECCCCHHHHEECCCCCCCCCCEEEEEEECCCCCCCCHHHHCCCC
NVALGGGDTVERHFMDTINGGKWLPNQDMAWRLCEQAVVRIRELENEIGCFFDRNADGSL
EEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCH
HQKAFAGQTADRTVHKGDLTGIEIINRLMEKVLASGVEKLQEHRAVGLIPTKDGSALAGV
HHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEE
LMIDMRTGGFRLVRAKTVMMGTGGGPTMYKYHTPSGDKTMDGLAMALRAGLPLRDMEMVQ
EEEEECCCCEEEEEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEE
FHPTGLLAGDHTRMTGTVLEEGLRGAGGQLISHAGNRFMFDYDGKGERATRDVVSRGIYA
ECCCEEEECCCCHHHHHHHHHHHCCCCCHHHHHCCCEEEEEECCCCCHHHHHHHHCCHHH
EMRKNNNPEQEGVFISMSHLGPEWVAQKFKGMVKRCADSGFDLAAGKVEVVPTAHYFMGG
HHHCCCCCCCCCEEEEHHHCCHHHHHHHHHHHHHHHHCCCCEEECCEEEEEECHHHHHCC
VVVDVDTRATMEGLYVAGEDAGGAHGSNRLGGNGVANSTVYGGIAGDTMGRDVGGMSLRE
EEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCC
PDEDVLAAEFERAIYPLSKKPDLVLPLRKQLQECMWEEVGVMRTEAGMQRGLAGIAEVSD
CCHHHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
ALMDVGVNGSNLAFNLTWHDWLNMASLCDISEVITRAGIARENSRGAHFREDFPEPGAME
HHHHCCCCCCCEEEEEEHHHHHCHHHHCCHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCC
DSEFTVARMEGGKVAVTREPVQFTIVRPGETVLPDDAPETLVAAQ
CCCEEEEEECCCEEEEEECCEEEEEECCCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.4 {fumarate}} 0.02 {succinate}} 0.3 {ferricyanide}} [C]

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]