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The map label for this gene is prpB [H]

Identifier: 89056258

GI number: 89056258

Start: 3849869

End: 3850738

Strand: Direct

Name: prpB [H]

Synonym: Jann_3767

Alternate gene names: 89056258

Gene position: 3849869-3850738 (Clockwise)

Preceding gene: 89056257

Following gene: 89056259

Centisome position: 89.16

GC content: 63.33

Gene sequence:

>870_bases
ATGAGCCTTCGCACGCGTCTTGAGGCGGATAACATTCTGGTCGCGCCCGGCGTTTATGACGGGCTGACGGCTGCCCTTGC
CCGTGATGCGGGGTTCGAGGCGTTGTATCTGTCCGGTGCCGCTGTGGCCTACACGCGATTGGGCCGCCCGGATATCGGGC
TGACCTCGGTGTCGGAAATGGCCGACACGATGGCGCTGATCCGGGACCGGGTGGATGTGCCGGTGATCATCGACGCCGAT
ACCGGCTTTGGCAACGCGCTGAACGCTCAACGCACCATGCGGCAATATGAGCGCGCGGGGGCCAATGCCTTGCAGGTTGA
GGATCAGACATATCCGAAACGCTGCGGCCACCTGTCCGACAAATCCCTGATCCCAACCGGTGAGATGGCGGGCAAGATTG
CCGCGATGGCCGACGCCCGTGCCTCGGACGAGACGCTGATCATCGCGCGCACCGACGCGGTCGCGGTCGAAGGCTTCGAC
GCCGCACTGACCCGCGCGGAGGCTTATATTGAGGCCGGGGCGGATGTGCTGTTCATCGAGGCACTGCGCTCGGAGGATGA
GATGCGATCCGTGACCGATCAGTTCCGGGGGCGCATCCCGCTATTGGCCAACATGGTCGAAGGCGGTGCGACACCCATCC
GCAGCGCCACAGATCTGGAAAAACTGGGATTCTCCATCGTGATCTTCCCGGGAGGCATTGTCCGCGCCATCGCCCGTACG
GCCGAGGCCTATTATGCCAGCCTGCACGAGGACGGAAGCAACCGGCCCTTTGCCGACCGGATGTTCGATTTCGACGGCCT
CAACGAGCGGATCGGGACGTCCGAGATGCTGATCAAGGGAAAGCGGTTCGAAGACAGCGGCAGATCCTGA

Upstream 100 bases:

>100_bases
GCTGCGCTGCTTTCAAGCAAGAGGTTCATGATGCCACGCTGATCTCATTGGGGTCGATTACGCCTGTCGTGACATGCGCC
GAGATGTTGGAGATCTTGGC

Downstream 100 bases:

>100_bases
CGATGTCGGTGCGGACCACACCGGATGCCGGGTTTGATCTGCACGTCCCTGTGCTGGTGATCGGTGCCGGGGCCTGCGGG
CTGGTGGCAGCACTTTCGGT

Product: 2,3-dimethylmalate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSLRTRLEADNILVAPGVYDGLTAALARDAGFEALYLSGAAVAYTRLGRPDIGLTSVSEMADTMALIRDRVDVPVIIDAD
TGFGNALNAQRTMRQYERAGANALQVEDQTYPKRCGHLSDKSLIPTGEMAGKIAAMADARASDETLIIARTDAVAVEGFD
AALTRAEAYIEAGADVLFIEALRSEDEMRSVTDQFRGRIPLLANMVEGGATPIRSATDLEKLGFSIVIFPGGIVRAIART
AEAYYASLHEDGSNRPFADRMFDFDGLNERIGTSEMLIKGKRFEDSGRS

Sequences:

>Translated_289_residues
MSLRTRLEADNILVAPGVYDGLTAALARDAGFEALYLSGAAVAYTRLGRPDIGLTSVSEMADTMALIRDRVDVPVIIDAD
TGFGNALNAQRTMRQYERAGANALQVEDQTYPKRCGHLSDKSLIPTGEMAGKIAAMADARASDETLIIARTDAVAVEGFD
AALTRAEAYIEAGADVLFIEALRSEDEMRSVTDQFRGRIPLLANMVEGGATPIRSATDLEKLGFSIVIFPGGIVRAIART
AEAYYASLHEDGSNRPFADRMFDFDGLNERIGTSEMLIKGKRFEDSGRS
>Mature_288_residues
SLRTRLEADNILVAPGVYDGLTAALARDAGFEALYLSGAAVAYTRLGRPDIGLTSVSEMADTMALIRDRVDVPVIIDADT
GFGNALNAQRTMRQYERAGANALQVEDQTYPKRCGHLSDKSLIPTGEMAGKIAAMADARASDETLIIARTDAVAVEGFDA
ALTRAEAYIEAGADVLFIEALRSEDEMRSVTDQFRGRIPLLANMVEGGATPIRSATDLEKLGFSIVIFPGGIVRAIARTA
EAYYASLHEDGSNRPFADRMFDFDGLNERIGTSEMLIKGKRFEDSGRS

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=272, Percent_Identity=38.6029411764706, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1790445, Length=166, Percent_Identity=33.1325301204819, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 31181; Mature: 31049

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRTRLEADNILVAPGVYDGLTAALARDAGFEALYLSGAAVAYTRLGRPDIGLTSVSEM
CCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHCCCCCCCHHHHHHH
ADTMALIRDRVDVPVIIDADTGFGNALNAQRTMRQYERAGANALQVEDQTYPKRCGHLSD
HHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHCCCCCC
KSLIPTGEMAGKIAAMADARASDETLIIARTDAVAVEGFDAALTRAEAYIEAGADVLFIE
CCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCEEEECCHHHHHHHHHHHHHCCCCEEEHH
ALRSEDEMRSVTDQFRGRIPLLANMVEGGATPIRSATDLEKLGFSIVIFPGGIVRAIART
HHHCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHCCCEEEEECCHHHHHHHHH
AEAYYASLHEDGSNRPFADRMFDFDGLNERIGTSEMLIKGKRFEDSGRS
HHHHHHHHHCCCCCCCHHHHHCCCCCCCHHCCCHHHHEECCCCCCCCCC
>Mature Secondary Structure 
SLRTRLEADNILVAPGVYDGLTAALARDAGFEALYLSGAAVAYTRLGRPDIGLTSVSEM
CCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHCCCCCCCHHHHHHH
ADTMALIRDRVDVPVIIDADTGFGNALNAQRTMRQYERAGANALQVEDQTYPKRCGHLSD
HHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHCCCCCC
KSLIPTGEMAGKIAAMADARASDETLIIARTDAVAVEGFDAALTRAEAYIEAGADVLFIE
CCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCEEEECCHHHHHHHHHHHHHCCCCEEEHH
ALRSEDEMRSVTDQFRGRIPLLANMVEGGATPIRSATDLEKLGFSIVIFPGGIVRAIART
HHHCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHCCCEEEEECCHHHHHHHHH
AEAYYASLHEDGSNRPFADRMFDFDGLNERIGTSEMLIKGKRFEDSGRS
HHHHHHHHHCCCCCCCHHHHHCCCCCCCHHCCCHHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]