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The map label for this gene is phaD [H]

Identifier: 89056208

GI number: 89056208

Start: 3802762

End: 3804381

Strand: Direct

Name: phaD [H]

Synonym: Jann_3717

Alternate gene names: 89056208

Gene position: 3802762-3804381 (Clockwise)

Preceding gene: 89056207

Following gene: 89056209

Centisome position: 88.07

GC content: 63.77

Gene sequence:

>1620_bases
ATGCATTGGATCATTCTTCCTGTCGTCCTGCCCGCCATGCTGGCGCCGCTGATCGCCTTCGCGATGCGACACGACATCAC
CTTGGCCCGCACGGCCTCAGTCGCAGGCACGTCTATTCTGCTGGCGATCGCGCTTGGTCTCGCGGCCCTTGCGGCGGACG
GCACGACCCATGTCTACAGGCTGGGGGATTGGCCCGCGCCGTTCGGCATTGTCCTGGTGCTCGACCGCCTGTCGGCCCTG
ATGGTGCTTCTGACCTCGACCCTGGCGATGGTGGTTCTGGTCCATGCGATCACGACCGGTTGGGACGCGCGGGGGCGTCA
TTTCCATGCCTTGTTTCAGTTTCAGCTCATGGGCATTTGCGGCGCATTCCTGACCGGCGACATCTTCAACCTCTTCGTGT
TCTTCGAGGTCTTGTTAATCGCCTCCTATGGCCTGATGATCCATTCCGGCGGGAAGGCACGGATGCGGGCGGGCCTGCAA
TATGTGGTCATGAACCTGGCCGGATCGACGCTGTTCCTGTTTGCTCTGGGCACGCTCTATGCCTCGACCGGAACGCTTAA
CATCGCAGATCTGGCCGTGCGTATCGCTGAGGTCCCGGTGGAAGAGGCAGCCCTTGTCCGCGTGGCGGTGATCTTGCTGC
TGATCGTCTTTGCGGTGAAAGCCGCGCTTTTCCCGGTGCAATTCTGGCTGCCCGCCACCTACGCCAACGCCCCTGCGCCT
GTGGCTGCCCTGTTTGCGATCATGACCAAGGTGGGCGCCTACGCGATCCTGCGAATTCACACGACGGCCTTCGGGCCGGG
CATCTCTGCCACGGAAGATCTGGCCGCGACGTGGCTGTTCCCGGCGGCCATCGTAACGATCGCCGTGGGAGCGATTGGCG
TGTTGGGCGCGCGCCGTCTGATGCCCTTGATCGCGTTTTCGGTTGTAGGCTCCATGGGAACCTTGCTGGTGGCTGTTGCG
GTCTTCTCGCAAACCGCAACGCTGGCGGCGCTGTACTACATGGTCCATTCGACTTTCGCGGCTGCTTGCCTGTTCCTCGT
GGCCGACCTGGTCGTCACACGCCGCGCCACGGATAGCCTCCAGCCAGAGCCGCCCACGGTCCAGAACGGCCTTTGCGCGG
CGCTGTTCTTTGCCGCCGCAATCGCCATGGCGGGCATGCCACCGCTCAGCGGCTTCCTCGGAAAACTGCTTGTCCTCGAC
GCCCTGCGGGAGCCGGGACTGATTGGATGGGCCTGGTCCACGATCCTAGTCGGGTCGTTGCTGACCATCGTGGGCTTCGC
ACGGGCGGGTAGCATCCTGTTCTGGAAATCGACGGCCACGGTGGGGCCTGCGGATGACAACGCCGACCCAGAGGCAGACC
AAACTGATGCCCCGGCGCAGCCCGTACCGGCAACGGCGGGACAAGTTGCCCCAGCCATGGTTGCCTTGGCCGGGCTCGCA
GCACTCGCGGCTTTCGCGGGGCCCGTCACCGCCTATCTGGAAGGGACGTCCGAGCAATTGTTTGACCGCGCAGGCTACGT
CGACGCCGTCCTGATCCCGATTGAACCTGAGGCTGCGGCGGACGCGACCGGTTATGGTAGTGAAGACCTTCACGGGGAAC
ACGACGAGGAGGAGCACTAG

Upstream 100 bases:

>100_bases
TTCTGGTGATGATCGGGCTTGGCGCTTATCTAGAATCGGACAGCGACCAAATCGATATCGAGCCGGAAAATGACGATCCC
CGCGACGAGGGGGGCGCGTG

Downstream 100 bases:

>100_bases
CCATGGTAAGACGCCTTGTCCCGCATCCCTTGCTCAGCCTGATGCTGATCCTCGTCTGGCTTGGCTTGGTGAACACAGTC
ACCCTGGGTAATCTGATCCT

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MHWIILPVVLPAMLAPLIAFAMRHDITLARTASVAGTSILLAIALGLAALAADGTTHVYRLGDWPAPFGIVLVLDRLSAL
MVLLTSTLAMVVLVHAITTGWDARGRHFHALFQFQLMGICGAFLTGDIFNLFVFFEVLLIASYGLMIHSGGKARMRAGLQ
YVVMNLAGSTLFLFALGTLYASTGTLNIADLAVRIAEVPVEEAALVRVAVILLLIVFAVKAALFPVQFWLPATYANAPAP
VAALFAIMTKVGAYAILRIHTTAFGPGISATEDLAATWLFPAAIVTIAVGAIGVLGARRLMPLIAFSVVGSMGTLLVAVA
VFSQTATLAALYYMVHSTFAAACLFLVADLVVTRRATDSLQPEPPTVQNGLCAALFFAAAIAMAGMPPLSGFLGKLLVLD
ALREPGLIGWAWSTILVGSLLTIVGFARAGSILFWKSTATVGPADDNADPEADQTDAPAQPVPATAGQVAPAMVALAGLA
ALAAFAGPVTAYLEGTSEQLFDRAGYVDAVLIPIEPEAAADATGYGSEDLHGEHDEEEH

Sequences:

>Translated_539_residues
MHWIILPVVLPAMLAPLIAFAMRHDITLARTASVAGTSILLAIALGLAALAADGTTHVYRLGDWPAPFGIVLVLDRLSAL
MVLLTSTLAMVVLVHAITTGWDARGRHFHALFQFQLMGICGAFLTGDIFNLFVFFEVLLIASYGLMIHSGGKARMRAGLQ
YVVMNLAGSTLFLFALGTLYASTGTLNIADLAVRIAEVPVEEAALVRVAVILLLIVFAVKAALFPVQFWLPATYANAPAP
VAALFAIMTKVGAYAILRIHTTAFGPGISATEDLAATWLFPAAIVTIAVGAIGVLGARRLMPLIAFSVVGSMGTLLVAVA
VFSQTATLAALYYMVHSTFAAACLFLVADLVVTRRATDSLQPEPPTVQNGLCAALFFAAAIAMAGMPPLSGFLGKLLVLD
ALREPGLIGWAWSTILVGSLLTIVGFARAGSILFWKSTATVGPADDNADPEADQTDAPAQPVPATAGQVAPAMVALAGLA
ALAAFAGPVTAYLEGTSEQLFDRAGYVDAVLIPIEPEAAADATGYGSEDLHGEHDEEEH
>Mature_539_residues
MHWIILPVVLPAMLAPLIAFAMRHDITLARTASVAGTSILLAIALGLAALAADGTTHVYRLGDWPAPFGIVLVLDRLSAL
MVLLTSTLAMVVLVHAITTGWDARGRHFHALFQFQLMGICGAFLTGDIFNLFVFFEVLLIASYGLMIHSGGKARMRAGLQ
YVVMNLAGSTLFLFALGTLYASTGTLNIADLAVRIAEVPVEEAALVRVAVILLLIVFAVKAALFPVQFWLPATYANAPAP
VAALFAIMTKVGAYAILRIHTTAFGPGISATEDLAATWLFPAAIVTIAVGAIGVLGARRLMPLIAFSVVGSMGTLLVAVA
VFSQTATLAALYYMVHSTFAAACLFLVADLVVTRRATDSLQPEPPTVQNGLCAALFFAAAIAMAGMPPLSGFLGKLLVLD
ALREPGLIGWAWSTILVGSLLTIVGFARAGSILFWKSTATVGPADDNADPEADQTDAPAQPVPATAGQVAPAMVALAGLA
ALAAFAGPVTAYLEGTSEQLFDRAGYVDAVLIPIEPEAAADATGYGSEDLHGEHDEEEH

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788613, Length=410, Percent_Identity=28.2926829268293, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1788831, Length=414, Percent_Identity=24.8792270531401, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788827, Length=373, Percent_Identity=26.2734584450402, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788829, Length=341, Percent_Identity=26.9794721407625, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI145693160, Length=389, Percent_Identity=22.6221079691517, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 56316; Mature: 56316

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHWIILPVVLPAMLAPLIAFAMRHDITLARTASVAGTSILLAIALGLAALAADGTTHVYR
CCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
LGDWPAPFGIVLVLDRLSALMVLLTSTLAMVVLVHAITTGWDARGRHFHALFQFQLMGIC
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
GAFLTGDIFNLFVFFEVLLIASYGLMIHSGGKARMRAGLQYVVMNLAGSTLFLFALGTLY
HHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ASTGTLNIADLAVRIAEVPVEEAALVRVAVILLLIVFAVKAALFPVQFWLPATYANAPAP
HCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
VAALFAIMTKVGAYAILRIHTTAFGPGISATEDLAATWLFPAAIVTIAVGAIGVLGARRL
HHHHHHHHHHHHHHEEEEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MPLIAFSVVGSMGTLLVAVAVFSQTATLAALYYMVHSTFAAACLFLVADLVVTRRATDSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QPEPPTVQNGLCAALFFAAAIAMAGMPPLSGFLGKLLVLDALREPGLIGWAWSTILVGSL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LTIVGFARAGSILFWKSTATVGPADDNADPEADQTDAPAQPVPATAGQVAPAMVALAGLA
HHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ALAAFAGPVTAYLEGTSEQLFDRAGYVDAVLIPIEPEAAADATGYGSEDLHGEHDEEEH
HHHHHHCCHHHHHCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHWIILPVVLPAMLAPLIAFAMRHDITLARTASVAGTSILLAIALGLAALAADGTTHVYR
CCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
LGDWPAPFGIVLVLDRLSALMVLLTSTLAMVVLVHAITTGWDARGRHFHALFQFQLMGIC
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
GAFLTGDIFNLFVFFEVLLIASYGLMIHSGGKARMRAGLQYVVMNLAGSTLFLFALGTLY
HHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ASTGTLNIADLAVRIAEVPVEEAALVRVAVILLLIVFAVKAALFPVQFWLPATYANAPAP
HCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
VAALFAIMTKVGAYAILRIHTTAFGPGISATEDLAATWLFPAAIVTIAVGAIGVLGARRL
HHHHHHHHHHHHHHEEEEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MPLIAFSVVGSMGTLLVAVAVFSQTATLAALYYMVHSTFAAACLFLVADLVVTRRATDSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QPEPPTVQNGLCAALFFAAAIAMAGMPPLSGFLGKLLVLDALREPGLIGWAWSTILVGSL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LTIVGFARAGSILFWKSTATVGPADDNADPEADQTDAPAQPVPATAGQVAPAMVALAGLA
HHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ALAAFAGPVTAYLEGTSEQLFDRAGYVDAVLIPIEPEAAADATGYGSEDLHGEHDEEEH
HHHHHHCCHHHHHCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]