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The map label for this gene is gsiB [H]

Identifier: 89055785

GI number: 89055785

Start: 3348360

End: 3350081

Strand: Direct

Name: gsiB [H]

Synonym: Jann_3294

Alternate gene names: 89055785

Gene position: 3348360-3350081 (Clockwise)

Preceding gene: 89055784

Following gene: 89055786

Centisome position: 77.54

GC content: 63.24

Gene sequence:

>1722_bases
ATGAAACGTACATCCATTCTTCTGGGGTGCGCCGCCGCGATGGCCTGCGCCCCCATGGCCGCGCTGGCCGACGGGCATCT
GTCCCGCGGCGGTTCCGGCCACCTCAACATTATCTATTGGCAAGCGCCCTCAACGCTGAACCCGTATCTGTCGGGCGGCA
CGAAAGAGGTCGAATCCGCCTCGCTGATCCTGGAAAGCCTGGGTCGCTTTGACGACACCGGCGAGCTGGTCCCATGGCTG
GCCGCCGAGATTCCGACCGTTGAGAACGGCGGCATCTCCGAGGATCTGACGCAGATCACCTGGACGCTGCAGGAAGGCAT
CCTGTGGTCCGATGGCACGCCCCTGACGGCGGCGGACGCCGTGTTCACGTGGGAGTATTGTACCGATTCCGAGAGTGGCT
GCGCCCAGGCGTCCTACTTTGACGGCGTCGAAGCGGTTGAGGCTGTGGACGACACGACGATCCAGATCACGTTCAACGCC
CCCACACCATTCCCCTACACCGCGCTGGTCGGCTCCGAGAGCCCAATCATCCAGGCCGCACAATTTGCCGATTGCGTCGG
CGCCCGCGCGCCTGAATGCACCGAGGCCAATTTCGGCCCCATCGGCACCGGTCCCTTCGTCGTCACGGACTTCCGTCCCA
ACGACGTGATCGAGCTTGTGGCCAACGACAATTACCGTGTGCCCGAGCAGCCCCACTTCGCGTCCGTGACCTTCAAAGGC
GGCGGCGATGCAACGGCGGCGGCGCGGTCCGTGCTGGAAACGGGCGAGTTTGACTACGCCTGGAACCTTCAGATCGACCC
GACAATCCTGGCGCAAATGGAAGCGCAGGGGAACGGCACGGTCGTGACCGCCTTCGGCACGTCCGTTGAGCGTCTGCACC
TGAACCAGACCAACCCCGATGCAGCGCTTGGCGACATGCGCTCCACCGTGGAAGGCGGGCCGCACCCGTTCCTGACCGAT
CCGATCGTGGGTCAGGCGATGTCCATGGCCATCGACCGCGCGCTGATCGTGGAAATCGGCTACGGGGCAGGCGGTCAGCC
CACCTGTAACGTCCTTCCCGCGCCCGAGGCCTATGCCTCCACCGCCAACGATGGCTGCCTTGTGCAGGACATCGCCGGTG
CCAATGCGCTGCTGGATGAAGCGGGCCACGTCGACACCGACGGCGACGGCATCCGCGAATACAACGGTGTTCCGATGACG
GTTCTTTATCAGACCTCGACCAACGCCGTCCGTCAGGACACCCAAGCGCTGGTGAAGCAATGGTGGGAAGAAATCGGCAT
CGGGTCCGAGCTTCGCAACATCGACGCATCGGTCTTCTTCGGTGGTGATCCGGCATCGCCCGACACGTTCCAGAAGTTCT
ATGCCGACGTCGAAATGTACACCAACAACTTCTCGGGCGTTGACCCGCAGGCGTATATGGCCAACTGGCTCTGCTCGGAC
ATTCCTGGTCCGGACACCCAATGGCAGGGTGCGAACATCCAGCGCTTCTGTTCCGAGGACTATGACGCCCTCGTGGCGGA
AATGGCCGGTACCGCAGACCTGGCCGAGCGGGGCCGTCTGGCGATGGCAATGAACGACATGATCATGCAGTCGTATTCCA
TCATCCCGCTGGTCCACCGTGGCGGCGTGTCGGCCCATGCCAACACTCTGGGCGGTGTGCGGATGTCCGATTGGGACACC
GAATTGTGGAACATCGCCGAATGGCACCGCATCCCTGAGTGA

Upstream 100 bases:

>100_bases
CCTGCCACCTGCACAGTTAAAAGCAACAAGATCGCCCCAAGGCGGCACGAGCAAACTGACAAAGACCGACCACGACCAAC
AAGGAGTGAGAGACACAGTA

Downstream 100 bases:

>100_bases
TGTGACCTGACTTCGGCCCGCCCCCCTTTGCCGGGGGGCGGGCCTTCCCCAGTTCGTGCGCAAGGTCACTTCGTTCCCAT
GCCGTCCCAACCCCTCCTCG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MKRTSILLGCAAAMACAPMAALADGHLSRGGSGHLNIIYWQAPSTLNPYLSGGTKEVESASLILESLGRFDDTGELVPWL
AAEIPTVENGGISEDLTQITWTLQEGILWSDGTPLTAADAVFTWEYCTDSESGCAQASYFDGVEAVEAVDDTTIQITFNA
PTPFPYTALVGSESPIIQAAQFADCVGARAPECTEANFGPIGTGPFVVTDFRPNDVIELVANDNYRVPEQPHFASVTFKG
GGDATAAARSVLETGEFDYAWNLQIDPTILAQMEAQGNGTVVTAFGTSVERLHLNQTNPDAALGDMRSTVEGGPHPFLTD
PIVGQAMSMAIDRALIVEIGYGAGGQPTCNVLPAPEAYASTANDGCLVQDIAGANALLDEAGHVDTDGDGIREYNGVPMT
VLYQTSTNAVRQDTQALVKQWWEEIGIGSELRNIDASVFFGGDPASPDTFQKFYADVEMYTNNFSGVDPQAYMANWLCSD
IPGPDTQWQGANIQRFCSEDYDALVAEMAGTADLAERGRLAMAMNDMIMQSYSIIPLVHRGGVSAHANTLGGVRMSDWDT
ELWNIAEWHRIPE

Sequences:

>Translated_573_residues
MKRTSILLGCAAAMACAPMAALADGHLSRGGSGHLNIIYWQAPSTLNPYLSGGTKEVESASLILESLGRFDDTGELVPWL
AAEIPTVENGGISEDLTQITWTLQEGILWSDGTPLTAADAVFTWEYCTDSESGCAQASYFDGVEAVEAVDDTTIQITFNA
PTPFPYTALVGSESPIIQAAQFADCVGARAPECTEANFGPIGTGPFVVTDFRPNDVIELVANDNYRVPEQPHFASVTFKG
GGDATAAARSVLETGEFDYAWNLQIDPTILAQMEAQGNGTVVTAFGTSVERLHLNQTNPDAALGDMRSTVEGGPHPFLTD
PIVGQAMSMAIDRALIVEIGYGAGGQPTCNVLPAPEAYASTANDGCLVQDIAGANALLDEAGHVDTDGDGIREYNGVPMT
VLYQTSTNAVRQDTQALVKQWWEEIGIGSELRNIDASVFFGGDPASPDTFQKFYADVEMYTNNFSGVDPQAYMANWLCSD
IPGPDTQWQGANIQRFCSEDYDALVAEMAGTADLAERGRLAMAMNDMIMQSYSIIPLVHRGGVSAHANTLGGVRMSDWDT
ELWNIAEWHRIPE
>Mature_573_residues
MKRTSILLGCAAAMACAPMAALADGHLSRGGSGHLNIIYWQAPSTLNPYLSGGTKEVESASLILESLGRFDDTGELVPWL
AAEIPTVENGGISEDLTQITWTLQEGILWSDGTPLTAADAVFTWEYCTDSESGCAQASYFDGVEAVEAVDDTTIQITFNA
PTPFPYTALVGSESPIIQAAQFADCVGARAPECTEANFGPIGTGPFVVTDFRPNDVIELVANDNYRVPEQPHFASVTFKG
GGDATAAARSVLETGEFDYAWNLQIDPTILAQMEAQGNGTVVTAFGTSVERLHLNQTNPDAALGDMRSTVEGGPHPFLTD
PIVGQAMSMAIDRALIVEIGYGAGGQPTCNVLPAPEAYASTANDGCLVQDIAGANALLDEAGHVDTDGDGIREYNGVPMT
VLYQTSTNAVRQDTQALVKQWWEEIGIGSELRNIDASVFFGGDPASPDTFQKFYADVEMYTNNFSGVDPQAYMANWLCSD
IPGPDTQWQGANIQRFCSEDYDALVAEMAGTADLAERGRLAMAMNDMIMQSYSIIPLVHRGGVSAHANTLGGVRMSDWDT
ELWNIAEWHRIPE

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=513, Percent_Identity=24.7563352826511, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789887, Length=400, Percent_Identity=24.75, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789966, Length=513, Percent_Identity=23.7816764132554, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787762, Length=220, Percent_Identity=27.7272727272727, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61333; Mature: 61333

Theoretical pI: Translated: 3.87; Mature: 3.87

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTSILLGCAAAMACAPMAALADGHLSRGGSGHLNIIYWQAPSTLNPYLSGGTKEVESA
CCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHCCCCHHHHHH
SLILESLGRFDDTGELVPWLAAEIPTVENGGISEDLTQITWTLQEGILWSDGTPLTAADA
HHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCCCEECCE
VFTWEYCTDSESGCAQASYFDGVEAVEAVDDTTIQITFNAPTPFPYTALVGSESPIIQAA
EEEEEECCCCCCCCCCHHHHCCHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCCHHHHH
QFADCVGARAPECTEANFGPIGTGPFVVTDFRPNDVIELVANDNYRVPEQPHFASVTFKG
HHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCEEEEEECC
GGDATAAARSVLETGEFDYAWNLQIDPTILAQMEAQGNGTVVTAFGTSVERLHLNQTNPD
CCCHHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCCEEEEEECCCEEEEEECCCCCC
AALGDMRSTVEGGPHPFLTDPIVGQAMSMAIDRALIVEIGYGAGGQPTCNVLPAPEAYAS
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCCHHHHC
TANDGCLVQDIAGANALLDEAGHVDTDGDGIREYNGVPMTVLYQTSTNAVRQDTQALVKQ
CCCCCEEEEECCCCHHHHHCCCCCCCCCCCCHHCCCCCEEEEEECCCHHHHHHHHHHHHH
WWEEIGIGSELRNIDASVFFGGDPASPDTFQKFYADVEMYTNNFSGVDPQAYMANWLCSD
HHHHHCCCCHHHCCCEEEEECCCCCCHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHC
IPGPDTQWQGANIQRFCSEDYDALVAEMAGTADLAERGRLAMAMNDMIMQSYSIIPLVHR
CCCCCCCCCCCCHHHHHCCHHHHHHHHHCCCHHHHHCCCEEEHHHHHHHHCCCEEEEEEC
GGVSAHANTLGGVRMSDWDTELWNIAEWHRIPE
CCCCCCCCCCCCCEECCCCCHHHCCHHHHCCCC
>Mature Secondary Structure
MKRTSILLGCAAAMACAPMAALADGHLSRGGSGHLNIIYWQAPSTLNPYLSGGTKEVESA
CCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHCCCCHHHHHH
SLILESLGRFDDTGELVPWLAAEIPTVENGGISEDLTQITWTLQEGILWSDGTPLTAADA
HHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCCCEECCE
VFTWEYCTDSESGCAQASYFDGVEAVEAVDDTTIQITFNAPTPFPYTALVGSESPIIQAA
EEEEEECCCCCCCCCCHHHHCCHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCCHHHHH
QFADCVGARAPECTEANFGPIGTGPFVVTDFRPNDVIELVANDNYRVPEQPHFASVTFKG
HHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCEEEEEECC
GGDATAAARSVLETGEFDYAWNLQIDPTILAQMEAQGNGTVVTAFGTSVERLHLNQTNPD
CCCHHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCCEEEEEECCCEEEEEECCCCCC
AALGDMRSTVEGGPHPFLTDPIVGQAMSMAIDRALIVEIGYGAGGQPTCNVLPAPEAYAS
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCCHHHHC
TANDGCLVQDIAGANALLDEAGHVDTDGDGIREYNGVPMTVLYQTSTNAVRQDTQALVKQ
CCCCCEEEEECCCCHHHHHCCCCCCCCCCCCHHCCCCCEEEEEECCCHHHHHHHHHHHHH
WWEEIGIGSELRNIDASVFFGGDPASPDTFQKFYADVEMYTNNFSGVDPQAYMANWLCSD
HHHHHCCCCHHHCCCEEEEECCCCCCHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHC
IPGPDTQWQGANIQRFCSEDYDALVAEMAGTADLAERGRLAMAMNDMIMQSYSIIPLVHR
CCCCCCCCCCCCHHHHHCCHHHHHHHHHCCCHHHHHCCCEEEHHHHHHHHCCCEEEEEEC
GGVSAHANTLGGVRMSDWDTELWNIAEWHRIPE
CCCCCCCCCCCCCEECCCCCHHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA