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The map label for this gene is potH [H]

Identifier: 89055726

GI number: 89055726

Start: 3283266

End: 3284090

Strand: Direct

Name: potH [H]

Synonym: Jann_3235

Alternate gene names: 89055726

Gene position: 3283266-3284090 (Clockwise)

Preceding gene: 89055725

Following gene: 89055727

Centisome position: 76.04

GC content: 62.42

Gene sequence:

>825_bases
ATGAAACGCCTTGCAGGCTGGATGTTGTCGTCACCTGCGACCCTTGCGGTGTCGCTGTTTCTGATCATTCCGGTGGCCGC
GACGATCAGCGCAACGTTCACCTCGCCCGGCGGCACCTTCGCGCCTTACGGCGAGTTTTTCGCGTCGGGCTTCCAGCGCA
CCGTTCTGTGGCGCACGTTTGAAGTAGCCGCGATCACCACTGTGATCTCGCTGATCCTGGGGCTGCTTACCGCCTATGTC
GTCTCCCGCAGCCCGCCCTGGATGAAGTCGGTTCTGGTCATTCTGGCGGTCTTCCCGCTGCTGACCGGGGTGGTCGTGCG
CTCCTTTGCCTGGCTGATCATTCTTGGACGCAACGGCGTGCTCAACAACACGCTGGAATGGTTGGGCGTGATCGAGCGGC
CCATGGCGCTGCTCTACAGCCAGACCTCTGTGGTCATCGCGCTGATCTACATCTTCATTCCCCTGATGATCCTGGTCCTG
ATCGGGGTGTTGGAGAACATACCCGACGATCTGCTCCAAGCCTCCTCCTCCCTCGGGGCGTCGCCCACGGCCACCTTTCT
GCAGATCACCCTGCCTCTGGCCACGCCCGGCCTGATCGTGGGGGCGGTGCTGGTCTTCACGGCCAGTTTCACCTCCTACG
CCACACCGCACCTTCTCGGCGGCACGCGGCAGATGATGATGGGCACGTTCCTGCACCAACGGGCCATGGTTTCCTTCGAC
TGGGTCAGTGCGTCGACCGTGGCGGCGATCATGGTCGTGGTGATCGTCACAACCGTGCTGGTCATGACGCGCATCGCCAA
GCGCCTGAACCCGATGGCATCGTGA

Upstream 100 bases:

>100_bases
CTGGAACGAAGTCTTCGGCATGTAAACGCGTGTTGGCCAGGACATTCCGGGGCGGATCCCTGCCCCGGAGATCTGGCCTC
TGCCCAACGGAAGCGCCCCA

Downstream 100 bases:

>100_bases
CATGACCCACCGCACCCACCCGCTTTTGATCGTGATGACCGTGCTGGTCTATTTCTTCCTGCTCGGGCCTTTGGTCATCA
TCTTCGGGGCGTCCGTCTCT

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MKRLAGWMLSSPATLAVSLFLIIPVAATISATFTSPGGTFAPYGEFFASGFQRTVLWRTFEVAAITTVISLILGLLTAYV
VSRSPPWMKSVLVILAVFPLLTGVVVRSFAWLIILGRNGVLNNTLEWLGVIERPMALLYSQTSVVIALIYIFIPLMILVL
IGVLENIPDDLLQASSSLGASPTATFLQITLPLATPGLIVGAVLVFTASFTSYATPHLLGGTRQMMMGTFLHQRAMVSFD
WVSASTVAAIMVVVIVTTVLVMTRIAKRLNPMAS

Sequences:

>Translated_274_residues
MKRLAGWMLSSPATLAVSLFLIIPVAATISATFTSPGGTFAPYGEFFASGFQRTVLWRTFEVAAITTVISLILGLLTAYV
VSRSPPWMKSVLVILAVFPLLTGVVVRSFAWLIILGRNGVLNNTLEWLGVIERPMALLYSQTSVVIALIYIFIPLMILVL
IGVLENIPDDLLQASSSLGASPTATFLQITLPLATPGLIVGAVLVFTASFTSYATPHLLGGTRQMMMGTFLHQRAMVSFD
WVSASTVAAIMVVVIVTTVLVMTRIAKRLNPMAS
>Mature_274_residues
MKRLAGWMLSSPATLAVSLFLIIPVAATISATFTSPGGTFAPYGEFFASGFQRTVLWRTFEVAAITTVISLILGLLTAYV
VSRSPPWMKSVLVILAVFPLLTGVVVRSFAWLIILGRNGVLNNTLEWLGVIERPMALLYSQTSVVIALIYIFIPLMILVL
IGVLENIPDDLLQASSSLGASPTATFLQITLPLATPGLIVGAVLVFTASFTSYATPHLLGGTRQMMMGTFLHQRAMVSFD
WVSASTVAAIMVVVIVTTVLVMTRIAKRLNPMAS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=211, Percent_Identity=35.5450236966825, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787369, Length=216, Percent_Identity=30.5555555555556, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1787713, Length=175, Percent_Identity=32, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29465; Mature: 29465

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLAGWMLSSPATLAVSLFLIIPVAATISATFTSPGGTFAPYGEFFASGFQRTVLWRTF
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHH
EVAAITTVISLILGLLTAYVVSRSPPWMKSVLVILAVFPLLTGVVVRSFAWLIILGRNGV
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
LNNTLEWLGVIERPMALLYSQTSVVIALIYIFIPLMILVLIGVLENIPDDLLQASSSLGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
SPTATFLQITLPLATPGLIVGAVLVFTASFTSYATPHLLGGTRQMMMGTFLHQRAMVSFD
CCCHHHEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHH
WVSASTVAAIMVVVIVTTVLVMTRIAKRLNPMAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKRLAGWMLSSPATLAVSLFLIIPVAATISATFTSPGGTFAPYGEFFASGFQRTVLWRTF
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHH
EVAAITTVISLILGLLTAYVVSRSPPWMKSVLVILAVFPLLTGVVVRSFAWLIILGRNGV
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
LNNTLEWLGVIERPMALLYSQTSVVIALIYIFIPLMILVLIGVLENIPDDLLQASSSLGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
SPTATFLQITLPLATPGLIVGAVLVFTASFTSYATPHLLGGTRQMMMGTFLHQRAMVSFD
CCCHHHEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHH
WVSASTVAAIMVVVIVTTVLVMTRIAKRLNPMAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]