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The map label for this gene is ptsP [H]

Identifier: 89055696

GI number: 89055696

Start: 3248214

End: 3250457

Strand: Direct

Name: ptsP [H]

Synonym: Jann_3205

Alternate gene names: 89055696

Gene position: 3248214-3250457 (Clockwise)

Preceding gene: 89055689

Following gene: 89055699

Centisome position: 75.23

GC content: 64.39

Gene sequence:

>2244_bases
ATGACCGAGAGTTTGGAAACAGAAAGCCGCAAGCTGCTCTCGCGCTTGCAGGCGGTCATGGCTGAGGCTGCGGCGGGTCA
GGAACGGCTGGACCGCATCACCCATCTGATCGCGGATTCGATGGGAACGGAAGTGTGCTCCATCTACCTGCTCCGCGATG
CCGAGACGTTGGAATTGTGTGCGACCCAGGGCCTGGCGCCCGAGGCTGTGCACGTCACGCGAATGCGGATCGGAGAGGGC
CTTGTGGGCCGCGTTGCGCTGCGGGCGCGCCCGTTCAACACCGCCAATGCCCCGGCCGAGAAGGGCTTCCGCTACATGGC
CGAGACCGGGGAAGAGGCGTTTTCCTCCTTCCTGGGCGTGCCGATCCAGCGGCTGGGAGAACGTCTGGGCGTGCTGGTCG
TTCAGTCCAAGGACGAGCGCGAGTATTCGGAGGACGAGGTCTACGCCCTTGAAGTCGTGGCCATGGTTCTGGCCGAGATG
ACAGAACTTGGAGCCTTCGTGGGCGAAGGGGCCGCCCTTTCGGCACGCCACACCAACCAGTTCATGTTTCGCGGCGGCGT
GGCCCAGGAAGGGGCCGCGATGGGGCATGTCTGGCTCCATGAGCCGCGCGTCGTCGTCACCAACCCCGTCGCCGATGACC
CGGCGGTCGAAAGGGTCCGTCTGACCGAGGCTATCGATCAGCTCCGCACCGATATCGACCAGATGCTGACCAATGTGTCG
CAGGGCGACAAGGATCAGGCCGAGGTGTTGGAGGCCTACCGCATGTTCGCCCGCTCTCGGGGCTGGATGCGCCGGATGGA
AGAGGACATCGAGCGGGGGCTGTCGGCAGAGGCGGCCGTGGAGAAAGAACAATCCTCGGCCCGCGCCCGGATGGAAACGG
TGCCCGACGCCTACCTGCGCGAACGCCTCCACGACCTTGATGATCTGTCCAACCGTCTGCTGCGTCTTCTGACTGGCCAA
GGCCGGGACACGGGCGCTGAGATGCCGATGGACCCGGTGCTTGTGGCCCGCAACATCGGCCCCGGAGAGCTGCTGGATTA
TGGTCGCAGTCTCAAAGGTGTCGTGTTGGAAGAAGGATCGGTGGGCTCGCACGCTGCGATTGTGGCCCGCGCGCTGGCGA
TCCCCTTGGTGATCCATGCCAAACGCATCAGCACGGAGGCGCTGAACGGCGACCCCATTCTGGTCGATGGCGACCAGGGG
ATCGTGCACTTGCGGCCCGAGGATACCGTCGCCTCTGCCTTCCGGGACAAACTGGCGATGCAGGCCGAAGCGCAGGAGCG
CTATGCCTCGATCCGCGACAAACCCGCGACATCGCTCTGCGGTACGACCGTGTCGCTGCAAATGAACGCGGGCCTGATGG
CGGATCTGCCGTCGCTGCCCTCGTCGGGTGCGGAGGGGGTGGGTCTTTTCCGGACGGAGTTGCAATTCCTCACCCGCGCC
CGCGTACCGCGCCGGGGCGAATTGGCGCGGCTCTACGCCAAGGTGATGGATGCTGCTGGCGACAAGCGCGTGGTGTTCCG
GACCCTCGACATCGGGTCGGACAAGGTGCTGCCCTACATGAAGCCCCAGGATGAGCCGAACCCGGCCCTTGGCTGGCGCG
CAATCCGTGTGGGTCTGGACAAACCCGGCGTCATGCGGATGCAGCTGCAAGCGCTGATCCGCGCCGCGAACGGCAGGCCC
TTGTCCGTGATGTTCCCCTTCATTGCCCAGTTCGATGAATTCACAGCCGCGCGCGATCACCTGATGCGCGAGATGGACCG
CGAGGCCGCCTTGGGCCATGTCCTGCCGTCTGACTTGCAAATTGGCGCGATGCTGGAAACGCCATCGCTGGCATTTGCCC
CGCATCAGTTTTTTGAACTGGCAGATTTCATCTCCATCGGGGGCAATGATCTCAAGCAGTTCTTCTTTGCCGCTGACCGG
GAAAATGAGCGCGTGCGCCGTCGCTATGACACGCTGAATGTCTCGTTCCTGACATTCATGGAGCAGATCGTGCACCGCTG
TGCGGAGACCGACACACCGCTGAGCTTCTGCGGAGAAGACGCAGGTCGTCCCGTGGAAGCAGTTTGTTTCGCTGCCATGG
GATTGCGCAACCTGTCGATGCGGCCCGCGTCAATCGGGCCGGTGAAGTCGCTTCTGCGGCGGGTGGATCTGCGCGAGGCC
CGGGGGGTGATCACCGAGGCACGCGGCGCGGGCGCGCAATCAGTCCGCGGGGCAGTGATGGATTGGCTGAAGCGCCAGGC
CTGA

Upstream 100 bases:

>100_bases
AGCGTTACTGCAACCATGCCGGTTCACAGCCATTGCAGGTCAAAGCCCCCCCGCGTTACATCTGGGCGATGGCGGCAGCA
CGCGGCCGGTGCGGGGACGG

Downstream 100 bases:

>100_bases
CGGATTGCGCGATGCGCGGCGACCGCATTCAGGATTGCGCTGCGCTTCACCAGCCGGGTCAGTTGCCCCGTCGCAAGGTG
GCGGTCAGGATCCCCATACC

Product: phosphoenolpyruvate-protein phosphotransferase PtsP

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 747; Mature: 746

Protein sequence:

>747_residues
MTESLETESRKLLSRLQAVMAEAAAGQERLDRITHLIADSMGTEVCSIYLLRDAETLELCATQGLAPEAVHVTRMRIGEG
LVGRVALRARPFNTANAPAEKGFRYMAETGEEAFSSFLGVPIQRLGERLGVLVVQSKDEREYSEDEVYALEVVAMVLAEM
TELGAFVGEGAALSARHTNQFMFRGGVAQEGAAMGHVWLHEPRVVVTNPVADDPAVERVRLTEAIDQLRTDIDQMLTNVS
QGDKDQAEVLEAYRMFARSRGWMRRMEEDIERGLSAEAAVEKEQSSARARMETVPDAYLRERLHDLDDLSNRLLRLLTGQ
GRDTGAEMPMDPVLVARNIGPGELLDYGRSLKGVVLEEGSVGSHAAIVARALAIPLVIHAKRISTEALNGDPILVDGDQG
IVHLRPEDTVASAFRDKLAMQAEAQERYASIRDKPATSLCGTTVSLQMNAGLMADLPSLPSSGAEGVGLFRTELQFLTRA
RVPRRGELARLYAKVMDAAGDKRVVFRTLDIGSDKVLPYMKPQDEPNPALGWRAIRVGLDKPGVMRMQLQALIRAANGRP
LSVMFPFIAQFDEFTAARDHLMREMDREAALGHVLPSDLQIGAMLETPSLAFAPHQFFELADFISIGGNDLKQFFFAADR
ENERVRRRYDTLNVSFLTFMEQIVHRCAETDTPLSFCGEDAGRPVEAVCFAAMGLRNLSMRPASIGPVKSLLRRVDLREA
RGVITEARGAGAQSVRGAVMDWLKRQA

Sequences:

>Translated_747_residues
MTESLETESRKLLSRLQAVMAEAAAGQERLDRITHLIADSMGTEVCSIYLLRDAETLELCATQGLAPEAVHVTRMRIGEG
LVGRVALRARPFNTANAPAEKGFRYMAETGEEAFSSFLGVPIQRLGERLGVLVVQSKDEREYSEDEVYALEVVAMVLAEM
TELGAFVGEGAALSARHTNQFMFRGGVAQEGAAMGHVWLHEPRVVVTNPVADDPAVERVRLTEAIDQLRTDIDQMLTNVS
QGDKDQAEVLEAYRMFARSRGWMRRMEEDIERGLSAEAAVEKEQSSARARMETVPDAYLRERLHDLDDLSNRLLRLLTGQ
GRDTGAEMPMDPVLVARNIGPGELLDYGRSLKGVVLEEGSVGSHAAIVARALAIPLVIHAKRISTEALNGDPILVDGDQG
IVHLRPEDTVASAFRDKLAMQAEAQERYASIRDKPATSLCGTTVSLQMNAGLMADLPSLPSSGAEGVGLFRTELQFLTRA
RVPRRGELARLYAKVMDAAGDKRVVFRTLDIGSDKVLPYMKPQDEPNPALGWRAIRVGLDKPGVMRMQLQALIRAANGRP
LSVMFPFIAQFDEFTAARDHLMREMDREAALGHVLPSDLQIGAMLETPSLAFAPHQFFELADFISIGGNDLKQFFFAADR
ENERVRRRYDTLNVSFLTFMEQIVHRCAETDTPLSFCGEDAGRPVEAVCFAAMGLRNLSMRPASIGPVKSLLRRVDLREA
RGVITEARGAGAQSVRGAVMDWLKRQA
>Mature_746_residues
TESLETESRKLLSRLQAVMAEAAAGQERLDRITHLIADSMGTEVCSIYLLRDAETLELCATQGLAPEAVHVTRMRIGEGL
VGRVALRARPFNTANAPAEKGFRYMAETGEEAFSSFLGVPIQRLGERLGVLVVQSKDEREYSEDEVYALEVVAMVLAEMT
ELGAFVGEGAALSARHTNQFMFRGGVAQEGAAMGHVWLHEPRVVVTNPVADDPAVERVRLTEAIDQLRTDIDQMLTNVSQ
GDKDQAEVLEAYRMFARSRGWMRRMEEDIERGLSAEAAVEKEQSSARARMETVPDAYLRERLHDLDDLSNRLLRLLTGQG
RDTGAEMPMDPVLVARNIGPGELLDYGRSLKGVVLEEGSVGSHAAIVARALAIPLVIHAKRISTEALNGDPILVDGDQGI
VHLRPEDTVASAFRDKLAMQAEAQERYASIRDKPATSLCGTTVSLQMNAGLMADLPSLPSSGAEGVGLFRTELQFLTRAR
VPRRGELARLYAKVMDAAGDKRVVFRTLDIGSDKVLPYMKPQDEPNPALGWRAIRVGLDKPGVMRMQLQALIRAANGRPL
SVMFPFIAQFDEFTAARDHLMREMDREAALGHVLPSDLQIGAMLETPSLAFAPHQFFELADFISIGGNDLKQFFFAADRE
NERVRRRYDTLNVSFLTFMEQIVHRCAETDTPLSFCGEDAGRPVEAVCFAAMGLRNLSMRPASIGPVKSLLRRVDLREAR
GVITEARGAGAQSVRGAVMDWLKRQA

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=751, Percent_Identity=32.4900133155792, Blast_Score=327, Evalue=2e-90,
Organism=Escherichia coli, GI1788756, Length=455, Percent_Identity=31.8681318681319, Blast_Score=247, Evalue=2e-66,
Organism=Escherichia coli, GI1788726, Length=557, Percent_Identity=29.802513464991, Blast_Score=230, Evalue=3e-61,
Organism=Escherichia coli, GI48994992, Length=490, Percent_Identity=28.7755102040816, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1787994, Length=469, Percent_Identity=24.3070362473348, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 82283; Mature: 82152

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESLETESRKLLSRLQAVMAEAAAGQERLDRITHLIADSMGTEVCSIYLLRDAETLELC
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHEEEEECCCHHHHHH
ATQGLAPEAVHVTRMRIGEGLVGRVALRARPFNTANAPAEKGFRYMAETGEEAFSSFLGV
HHCCCCCHHHHHHHHHHCCCHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PIQRLGERLGVLVVQSKDEREYSEDEVYALEVVAMVLAEMTELGAFVGEGAALSARHTNQ
HHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCH
FMFRGGVAQEGAAMGHVWLHEPRVVVTNPVADDPAVERVRLTEAIDQLRTDIDQMLTNVS
HHEECCCCCCCCCCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
QGDKDQAEVLEAYRMFARSRGWMRRMEEDIERGLSAEAAVEKEQSSARARMETVPDAYLR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHH
ERLHDLDDLSNRLLRLLTGQGRDTGAEMPMDPVLVARNIGPGELLDYGRSLKGVVLEEGS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHCCCCCCEEEECCC
VGSHAAIVARALAIPLVIHAKRISTEALNGDPILVDGDQGIVHLRPEDTVASAFRDKLAM
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHH
QAEAQERYASIRDKPATSLCGTTVSLQMNAGLMADLPSLPSSGAEGVGLFRTELQFLTRA
HHHHHHHHHHHCCCCCHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
RVPRRGELARLYAKVMDAAGDKRVVFRTLDIGSDKVLPYMKPQDEPNPALGWRAIRVGLD
CCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCC
KPGVMRMQLQALIRAANGRPLSVMFPFIAQFDEFTAARDHLMREMDREAALGHVLPSDLQ
CCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IGAMLETPSLAFAPHQFFELADFISIGGNDLKQFFFAADRENERVRRRYDTLNVSFLTFM
CCEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHH
EQIVHRCAETDTPLSFCGEDAGRPVEAVCFAAMGLRNLSMRPASIGPVKSLLRRVDLREA
HHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
RGVITEARGAGAQSVRGAVMDWLKRQA
HCHHHHCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TESLETESRKLLSRLQAVMAEAAAGQERLDRITHLIADSMGTEVCSIYLLRDAETLELC
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHEEEEECCCHHHHHH
ATQGLAPEAVHVTRMRIGEGLVGRVALRARPFNTANAPAEKGFRYMAETGEEAFSSFLGV
HHCCCCCHHHHHHHHHHCCCHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PIQRLGERLGVLVVQSKDEREYSEDEVYALEVVAMVLAEMTELGAFVGEGAALSARHTNQ
HHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCH
FMFRGGVAQEGAAMGHVWLHEPRVVVTNPVADDPAVERVRLTEAIDQLRTDIDQMLTNVS
HHEECCCCCCCCCCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
QGDKDQAEVLEAYRMFARSRGWMRRMEEDIERGLSAEAAVEKEQSSARARMETVPDAYLR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHH
ERLHDLDDLSNRLLRLLTGQGRDTGAEMPMDPVLVARNIGPGELLDYGRSLKGVVLEEGS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHCCCCCCEEEECCC
VGSHAAIVARALAIPLVIHAKRISTEALNGDPILVDGDQGIVHLRPEDTVASAFRDKLAM
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHH
QAEAQERYASIRDKPATSLCGTTVSLQMNAGLMADLPSLPSSGAEGVGLFRTELQFLTRA
HHHHHHHHHHHCCCCCHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
RVPRRGELARLYAKVMDAAGDKRVVFRTLDIGSDKVLPYMKPQDEPNPALGWRAIRVGLD
CCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCC
KPGVMRMQLQALIRAANGRPLSVMFPFIAQFDEFTAARDHLMREMDREAALGHVLPSDLQ
CCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IGAMLETPSLAFAPHQFFELADFISIGGNDLKQFFFAADRENERVRRRYDTLNVSFLTFM
CCEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHH
EQIVHRCAETDTPLSFCGEDAGRPVEAVCFAAMGLRNLSMRPASIGPVKSLLRRVDLREA
HHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
RGVITEARGAGAQSVRGAVMDWLKRQA
HCHHHHCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]