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The map label for this gene is pleD [H]

Identifier: 89055622

GI number: 89055622

Start: 3172171

End: 3173565

Strand: Direct

Name: pleD [H]

Synonym: Jann_3131

Alternate gene names: 89055622

Gene position: 3172171-3173565 (Clockwise)

Preceding gene: 89055610

Following gene: 89055627

Centisome position: 73.46

GC content: 64.87

Gene sequence:

>1395_bases
ATGGTAGGGCGTATTCTTGTCGTTGACGACGTTGCGACCAACCGGATCGTGATGAAAGTGAAACTCACTGCGGCCTGCTA
CTCGGTGGATCAGGCCGAAAACGGGGCCGCTGCCCTCAAGGCCGCGCGCGAGACGAAGCCGGATCTCATCCTTCTGGACG
TCATGATGCCGGACATGAGCGGGCTGGACGTCTGTCGCGCGCTCAAAGCCGACCCTGAGACCGCAGATATTCCCGTGGTC
CTGATCACCGCCCTCTTTGATCAAGCCGCCAAGATGGATGGGCTGGAGGCGGGGGCCGATGATTTCCTGACCAAGCCGGT
GGAGGAGGTGACGCTGCTTGCGCGGGTGCGCTCGCTCCTGCGGGTCAGCGATTCCGTGCGCGAATTGCGCGCCCGTGATG
ATACCGTGTCAGCCTACGGTTTTGCCGAGGCCGCGCAGGGGTTTGAGGGCAAGGCACGCCCCGGCCATGTGGCGCTTGTG
GCGCCGGGGCCGCGCGGTGCGGTCCTGTGGAAGGCCGCCATTGATGACCGTATCGGTGGCGAGGTTCAGATCATTCCGCG
TGAGACGGCCCTGACCCAGGCCACCGGCACTGGCAACGACCCCGATGTCTTCGTGATCTCGGTCGATCTGGCGCAACGCA
ATGAAGGGCTTCGCCTGCTCTCGGATCTGCGGTCCCGCCCCGGCACGCGCCACTCCGCGATCGTGATGATCCTGCCCGAG
GGCGATAGCGAGCGGGCCGCGATTGCGCTCGATCTGGGGGCCAACGATGTGCTCCACGATCCGTTTGACGCGCAAGAACT
GGCCATTCGTATCTCTGCACAGCTTGATCGCAAGCGGCAATCGGACCGGATGCGCGCGGGCGTGCGCGCCAGTCTGGAGG
CCGCGATCACCGATCCGCTCACCGGCCTTTACAACCGTCGCTTCGCGCTGCACCGGATGGAGCAGATCATGGCGCGGCCC
GGCGGCGATCCGGCGGTCATGATGCTGGATCTCGATTACTTCAAGCAGATCAACGACACCTATGGCCATGCGGCGGGCGA
TGCTGTGTTGCAAGAGGTCGCGATCCGGCTGCGCGGGCAGGTTCAGGCCAATGACCTTGTTGCGCGTATCGGTGGAGAAG
AGTTTCTGGTCGTCCTGTCCGGAGCCAGTGCGCGGGCCGCGACGGAATGTGCCGAGCGGTTGCGCGCCTGCATCGGCGGG
ATGAGCTTTGACCTGGGTGGCGACACCTTCCCCGTCCGCGTGACCGCCAGTGTTGGTCTGTCCCTGCCGGGCAGTTCTGA
TCCTCACACGACCAGCACGCCGGAGGCATTGATCCACAAGGCCGACACGGCGCTCTATGGCGCCAAGGCCCATGGCCGCG
ATCAGGTCAGCCAGATGTGCGACACCGCCGCCTAG

Upstream 100 bases:

>100_bases
CAGCTTAAAGAATTTGTTAACTAATCGGGTGGTAAGCCTAACAACACATTAAGGTCTGACGGACCTGTATGTCGGTGTCA
AACGAGGTGAGGTATACATC

Downstream 100 bases:

>100_bases
TCGGGACACGCATGGCCAGTGCGGTCGGGCCTATTGCCGTCTGCCCACACGCCGCATCACCCGCGCCAGATGATTGGCCA
GATCCTGGCGCTCTGCCGCG

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MVGRILVVDDVATNRIVMKVKLTAACYSVDQAENGAAALKAARETKPDLILLDVMMPDMSGLDVCRALKADPETADIPVV
LITALFDQAAKMDGLEAGADDFLTKPVEEVTLLARVRSLLRVSDSVRELRARDDTVSAYGFAEAAQGFEGKARPGHVALV
APGPRGAVLWKAAIDDRIGGEVQIIPRETALTQATGTGNDPDVFVISVDLAQRNEGLRLLSDLRSRPGTRHSAIVMILPE
GDSERAAIALDLGANDVLHDPFDAQELAIRISAQLDRKRQSDRMRAGVRASLEAAITDPLTGLYNRRFALHRMEQIMARP
GGDPAVMMLDLDYFKQINDTYGHAAGDAVLQEVAIRLRGQVQANDLVARIGGEEFLVVLSGASARAATECAERLRACIGG
MSFDLGGDTFPVRVTASVGLSLPGSSDPHTTSTPEALIHKADTALYGAKAHGRDQVSQMCDTAA

Sequences:

>Translated_464_residues
MVGRILVVDDVATNRIVMKVKLTAACYSVDQAENGAAALKAARETKPDLILLDVMMPDMSGLDVCRALKADPETADIPVV
LITALFDQAAKMDGLEAGADDFLTKPVEEVTLLARVRSLLRVSDSVRELRARDDTVSAYGFAEAAQGFEGKARPGHVALV
APGPRGAVLWKAAIDDRIGGEVQIIPRETALTQATGTGNDPDVFVISVDLAQRNEGLRLLSDLRSRPGTRHSAIVMILPE
GDSERAAIALDLGANDVLHDPFDAQELAIRISAQLDRKRQSDRMRAGVRASLEAAITDPLTGLYNRRFALHRMEQIMARP
GGDPAVMMLDLDYFKQINDTYGHAAGDAVLQEVAIRLRGQVQANDLVARIGGEEFLVVLSGASARAATECAERLRACIGG
MSFDLGGDTFPVRVTASVGLSLPGSSDPHTTSTPEALIHKADTALYGAKAHGRDQVSQMCDTAA
>Mature_464_residues
MVGRILVVDDVATNRIVMKVKLTAACYSVDQAENGAAALKAARETKPDLILLDVMMPDMSGLDVCRALKADPETADIPVV
LITALFDQAAKMDGLEAGADDFLTKPVEEVTLLARVRSLLRVSDSVRELRARDDTVSAYGFAEAAQGFEGKARPGHVALV
APGPRGAVLWKAAIDDRIGGEVQIIPRETALTQATGTGNDPDVFVISVDLAQRNEGLRLLSDLRSRPGTRHSAIVMILPE
GDSERAAIALDLGANDVLHDPFDAQELAIRISAQLDRKRQSDRMRAGVRASLEAAITDPLTGLYNRRFALHRMEQIMARP
GGDPAVMMLDLDYFKQINDTYGHAAGDAVLQEVAIRLRGQVQANDLVARIGGEEFLVVLSGASARAATECAERLRACIGG
MSFDLGGDTFPVRVTASVGLSLPGSSDPHTTSTPEALIHKADTALYGAKAHGRDQVSQMCDTAA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=172, Percent_Identity=33.7209302325581, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=35.8024691358025, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI87082007, Length=173, Percent_Identity=39.3063583815029, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788394, Length=130, Percent_Identity=38.4615384615385, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786599, Length=121, Percent_Identity=40.495867768595, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=40.6779661016949, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787541, Length=209, Percent_Identity=32.0574162679426, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1787802, Length=162, Percent_Identity=34.5679012345679, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081977, Length=192, Percent_Identity=31.7708333333333, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=34.3023255813954, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788085, Length=171, Percent_Identity=30.9941520467836, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786584, Length=161, Percent_Identity=32.2981366459627, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788381, Length=176, Percent_Identity=31.8181818181818, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790860, Length=95, Percent_Identity=41.0526315789474, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787229, Length=142, Percent_Identity=33.0985915492958, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789809, Length=118, Percent_Identity=33.8983050847458, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=31.4465408805031, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787375, Length=118, Percent_Identity=34.7457627118644, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1790863, Length=137, Percent_Identity=30.6569343065693, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081974, Length=102, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI48994928, Length=123, Percent_Identity=38.2113821138211, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788956, Length=194, Percent_Identity=30.9278350515464, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082012, Length=205, Percent_Identity=27.3170731707317, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787056, Length=178, Percent_Identity=29.7752808988764, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790299, Length=147, Percent_Identity=32.6530612244898, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786911, Length=117, Percent_Identity=35.042735042735, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788550, Length=142, Percent_Identity=27.4647887323944, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790437, Length=160, Percent_Identity=31.875, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789149, Length=116, Percent_Identity=32.7586206896552, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI2367329, Length=133, Percent_Identity=36.0902255639098, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 49553; Mature: 49553

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGRILVVDDVATNRIVMKVKLTAACYSVDQAENGAAALKAARETKPDLILLDVMMPDMS
CCCEEEEEECCCCCEEEEEEEEEHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEECCCCC
GLDVCRALKADPETADIPVVLITALFDQAAKMDGLEAGADDFLTKPVEEVTLLARVRSLL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHH
RVSDSVRELRARDDTVSAYGFAEAAQGFEGKARPGHVALVAPGPRGAVLWKAAIDDRIGG
HHHHHHHHHHHCCCCCHHHCHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEEHHCCCCCC
EVQIIPRETALTQATGTGNDPDVFVISVDLAQRNEGLRLLSDLRSRPGTRHSAIVMILPE
EEEEEECHHHHEECCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEEEEC
GDSERAAIALDLGANDVLHDPFDAQELAIRISAQLDRKRQSDRMRAGVRASLEAAITDPL
CCCCCEEEEEECCCCCCCCCCCCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
TGLYNRRFALHRMEQIMARPGGDPAVMMLDLDYFKQINDTYGHAAGDAVLQEVAIRLRGQ
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
VQANDLVARIGGEEFLVVLSGASARAATECAERLRACIGGMSFDLGGDTFPVRVTASVGL
CCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEECCCCEEEEEEEEEECE
SLPGSSDPHTTSTPEALIHKADTALYGAKAHGRDQVSQMCDTAA
ECCCCCCCCCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MVGRILVVDDVATNRIVMKVKLTAACYSVDQAENGAAALKAARETKPDLILLDVMMPDMS
CCCEEEEEECCCCCEEEEEEEEEHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEECCCCC
GLDVCRALKADPETADIPVVLITALFDQAAKMDGLEAGADDFLTKPVEEVTLLARVRSLL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHH
RVSDSVRELRARDDTVSAYGFAEAAQGFEGKARPGHVALVAPGPRGAVLWKAAIDDRIGG
HHHHHHHHHHHCCCCCHHHCHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEEHHCCCCCC
EVQIIPRETALTQATGTGNDPDVFVISVDLAQRNEGLRLLSDLRSRPGTRHSAIVMILPE
EEEEEECHHHHEECCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEEEEC
GDSERAAIALDLGANDVLHDPFDAQELAIRISAQLDRKRQSDRMRAGVRASLEAAITDPL
CCCCCEEEEEECCCCCCCCCCCCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
TGLYNRRFALHRMEQIMARPGGDPAVMMLDLDYFKQINDTYGHAAGDAVLQEVAIRLRGQ
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
VQANDLVARIGGEEFLVVLSGASARAATECAERLRACIGGMSFDLGGDTFPVRVTASVGL
CCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEECCCCEEEEEEEEEECE
SLPGSSDPHTTSTPEALIHKADTALYGAKAHGRDQVSQMCDTAA
ECCCCCCCCCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA