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The map label for this gene is dppA [H]

Identifier: 89055313

GI number: 89055313

Start: 2836605

End: 2838158

Strand: Direct

Name: dppA [H]

Synonym: Jann_2822

Alternate gene names: 89055313

Gene position: 2836605-2838158 (Clockwise)

Preceding gene: 89055312

Following gene: 89055315

Centisome position: 65.69

GC content: 64.35

Gene sequence:

>1554_bases
ATGAACCGCTTATTCACTGCCAGCGTGCTGGCCATGGCCACCGCGATCCCGGCCCAGGCCGAAACCTTCCGGTGGGCCGC
CACGACCGACCCGCAGACCATGGACCCCCACGCGGTGTCCTCCGCCCCGGTTCTGGGGTTCCTGAACAACGTCTATGAGG
GCCTCGTGCGGCGCGGCCGCGACATGTCCATCGAACCCGCTTTGGCCGAGGCGTGGGAACCACTGCCCGACGGCGCGGGT
TGGCGCTTCACCCTGCGCCAGGGCGTGACCTTCCATGATGGCGCGGCCTTCAACGCCGATGACGTGCTGTTCTCCTACGA
GCGCGCCAGTTCCGAGGACAGCGACGTGGCCAGCTGGTTCGCCACCGTCTCGGGCGTCGAGATCGTGGATGAGTTCACCG
TCGATATCCTGACAACGACGCCGCAGCCGATCTTTCCCGACAGCATCGCCAACTGGATGATGATGGACAGCGACTGGGCC
GCCGCCAATGGCGCGGAACGTCCCGCCCGCGATGCGGAGAATTTCGCGACCCGCAACGTCAACGGCACCGCCGCGTTCAT
GCTGCAATCGCGCCAGCCCGATCTGGAGACTGTCCTTGTCCCCTTCGACGGCTGGTGGGGCGAGGTCGAGCATAATGTGA
CCGAGGCGATCTTCACGCCGATCCAGAACTCCGCCACCGCCGTGGCCGCGTTGCTTTCGGGCGATATCGACCTGATCGAC
CCGGTGCCGGTCCAGGACGCGGACCGCGTGGACGCCGCTGACGGCGTCTATGTCCTGTCGGGGATCGAGGCGCGGGTGAT
CATGTTGGGCTACGGCCACGACCATGAGACGCTTCTGAACGGCAACGACAACATCTTCTCGGATGTGCGTGTGCGTCAGG
CCGTGGCCCACGCCGTCAATGTCCCCGCGATCCTGCAAGCGATCATGGGCGGCAACGCGGAGGAGGCGAGCCAATTGGTG
AGCCCAGCCATGCGCGGCTATTCCGAGGCCCGCGCAGACCGGCCCGCCTTTGATCAGGATCTGGCGCGGTCCCTGATTGC
CGAGGCCGGGGCAGAAGGCGCCGAGTTCAACCTGTCCTGCCCCAATGACCGATACCTCAATGATGAGGCCGTCTGTCAGG
CCATCGTCGGCATGCTGGCCCAGGTCGGCCTGAATGCGCAGCTGGAGACGATGCCGGTCAGCAATTACTGGCCCGAGCTG
CGTGCCGACAATTACGACATGTACCTTCTCGGTTGGTCACCTGGCACGTTCGATGCCGAGCATCCGCTGCGCTTCCTTGT
TCACACCAATACTGAGAACCTCGGGACGTGGAACTTCGGCGGATATTCCAACCCCCGCATCGACGAGATGCTGCCGCTGA
TCCAGTCCGAGATTGACGAAGATGCGCGTCAGGCCATGCTGGATGAGGCCGCGGCCATCGTGCAGGACGATGCGGTCTAC
AACACGATGTATGTCCAGCCTCTGCTCTGGGGTGTGGCCAATGGCATCGAGCTGACCCAGCGCCCCGACAACTTTTTCAT
CCTGCGGTGGGTGACGGTCGGGGCCGAAAACTAG

Upstream 100 bases:

>100_bases
CGCCGCCCGATCGGCCCTTGCCGCCCTCCCCCCGCCCCGGCTAGGCTCCTCCGCGCGGCGGACCGGGGTCGCGTGATGAT
TTTCTGGGGAGAGACACCAA

Downstream 100 bases:

>100_bases
GGAGAGAGATTACGCTTGGTCCCGGCCTGAGGGCCGGACCAAACGCCTGATGCGGGGGGCAAGCCCCCCGCGCGCGCCAA
GCCCTCCTGCCAAACACCTG

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MNRLFTASVLAMATAIPAQAETFRWAATTDPQTMDPHAVSSAPVLGFLNNVYEGLVRRGRDMSIEPALAEAWEPLPDGAG
WRFTLRQGVTFHDGAAFNADDVLFSYERASSEDSDVASWFATVSGVEIVDEFTVDILTTTPQPIFPDSIANWMMMDSDWA
AANGAERPARDAENFATRNVNGTAAFMLQSRQPDLETVLVPFDGWWGEVEHNVTEAIFTPIQNSATAVAALLSGDIDLID
PVPVQDADRVDAADGVYVLSGIEARVIMLGYGHDHETLLNGNDNIFSDVRVRQAVAHAVNVPAILQAIMGGNAEEASQLV
SPAMRGYSEARADRPAFDQDLARSLIAEAGAEGAEFNLSCPNDRYLNDEAVCQAIVGMLAQVGLNAQLETMPVSNYWPEL
RADNYDMYLLGWSPGTFDAEHPLRFLVHTNTENLGTWNFGGYSNPRIDEMLPLIQSEIDEDARQAMLDEAAAIVQDDAVY
NTMYVQPLLWGVANGIELTQRPDNFFILRWVTVGAEN

Sequences:

>Translated_517_residues
MNRLFTASVLAMATAIPAQAETFRWAATTDPQTMDPHAVSSAPVLGFLNNVYEGLVRRGRDMSIEPALAEAWEPLPDGAG
WRFTLRQGVTFHDGAAFNADDVLFSYERASSEDSDVASWFATVSGVEIVDEFTVDILTTTPQPIFPDSIANWMMMDSDWA
AANGAERPARDAENFATRNVNGTAAFMLQSRQPDLETVLVPFDGWWGEVEHNVTEAIFTPIQNSATAVAALLSGDIDLID
PVPVQDADRVDAADGVYVLSGIEARVIMLGYGHDHETLLNGNDNIFSDVRVRQAVAHAVNVPAILQAIMGGNAEEASQLV
SPAMRGYSEARADRPAFDQDLARSLIAEAGAEGAEFNLSCPNDRYLNDEAVCQAIVGMLAQVGLNAQLETMPVSNYWPEL
RADNYDMYLLGWSPGTFDAEHPLRFLVHTNTENLGTWNFGGYSNPRIDEMLPLIQSEIDEDARQAMLDEAAAIVQDDAVY
NTMYVQPLLWGVANGIELTQRPDNFFILRWVTVGAEN
>Mature_517_residues
MNRLFTASVLAMATAIPAQAETFRWAATTDPQTMDPHAVSSAPVLGFLNNVYEGLVRRGRDMSIEPALAEAWEPLPDGAG
WRFTLRQGVTFHDGAAFNADDVLFSYERASSEDSDVASWFATVSGVEIVDEFTVDILTTTPQPIFPDSIANWMMMDSDWA
AANGAERPARDAENFATRNVNGTAAFMLQSRQPDLETVLVPFDGWWGEVEHNVTEAIFTPIQNSATAVAALLSGDIDLID
PVPVQDADRVDAADGVYVLSGIEARVIMLGYGHDHETLLNGNDNIFSDVRVRQAVAHAVNVPAILQAIMGGNAEEASQLV
SPAMRGYSEARADRPAFDQDLARSLIAEAGAEGAEFNLSCPNDRYLNDEAVCQAIVGMLAQVGLNAQLETMPVSNYWPEL
RADNYDMYLLGWSPGTFDAEHPLRFLVHTNTENLGTWNFGGYSNPRIDEMLPLIQSEIDEDARQAMLDEAAAIVQDDAVY
NTMYVQPLLWGVANGIELTQRPDNFFILRWVTVGAEN

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=500, Percent_Identity=25.6, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1787762, Length=466, Percent_Identity=26.1802575107296, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1787052, Length=465, Percent_Identity=23.4408602150538, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789397, Length=517, Percent_Identity=24.7582205029014, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87081878, Length=480, Percent_Identity=21.4583333333333, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1787495, Length=539, Percent_Identity=22.2634508348794, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1789887, Length=494, Percent_Identity=22.4696356275304, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56616; Mature: 56616

Theoretical pI: Translated: 3.93; Mature: 3.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLFTASVLAMATAIPAQAETFRWAATTDPQTMDPHAVSSAPVLGFLNNVYEGLVRRGR
CCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
DMSIEPALAEAWEPLPDGAGWRFTLRQGVTFHDGAAFNADDVLFSYERASSEDSDVASWF
CCCCCCHHHHHCCCCCCCCCEEEEEECCCEECCCCCCCCCHHEEEEHHCCCCCHHHHHHH
ATVSGVEIVDEFTVDILTTTPQPIFPDSIANWMMMDSDWAAANGAERPARDAENFATRNV
HHHCCHHHHHHHEEEEEECCCCCCCCHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHCCC
NGTAAFMLQSRQPDLETVLVPFDGWWGEVEHNVTEAIFTPIQNSATAVAALLSGDIDLID
CCEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
PVPVQDADRVDAADGVYVLSGIEARVIMLGYGHDHETLLNGNDNIFSDVRVRQAVAHAVN
CCCCCCCCCCCCCCCEEEEECCCEEEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHCC
VPAILQAIMGGNAEEASQLVSPAMRGYSEARADRPAFDQDLARSLIAEAGAEGAEFNLSC
HHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEECCC
PNDRYLNDEAVCQAIVGMLAQVGLNAQLETMPVSNYWPELRADNYDMYLLGWSPGTFDAE
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCC
HPLRFLVHTNTENLGTWNFGGYSNPRIDEMLPLIQSEIDEDARQAMLDEAAAIVQDDAVY
CCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
NTMYVQPLLWGVANGIELTQRPDNFFILRWVTVGAEN
HHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEECCCC
>Mature Secondary Structure
MNRLFTASVLAMATAIPAQAETFRWAATTDPQTMDPHAVSSAPVLGFLNNVYEGLVRRGR
CCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
DMSIEPALAEAWEPLPDGAGWRFTLRQGVTFHDGAAFNADDVLFSYERASSEDSDVASWF
CCCCCCHHHHHCCCCCCCCCEEEEEECCCEECCCCCCCCCHHEEEEHHCCCCCHHHHHHH
ATVSGVEIVDEFTVDILTTTPQPIFPDSIANWMMMDSDWAAANGAERPARDAENFATRNV
HHHCCHHHHHHHEEEEEECCCCCCCCHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHCCC
NGTAAFMLQSRQPDLETVLVPFDGWWGEVEHNVTEAIFTPIQNSATAVAALLSGDIDLID
CCEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
PVPVQDADRVDAADGVYVLSGIEARVIMLGYGHDHETLLNGNDNIFSDVRVRQAVAHAVN
CCCCCCCCCCCCCCCEEEEECCCEEEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHCC
VPAILQAIMGGNAEEASQLVSPAMRGYSEARADRPAFDQDLARSLIAEAGAEGAEFNLSC
HHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEECCC
PNDRYLNDEAVCQAIVGMLAQVGLNAQLETMPVSNYWPELRADNYDMYLLGWSPGTFDAE
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCC
HPLRFLVHTNTENLGTWNFGGYSNPRIDEMLPLIQSEIDEDARQAMLDEAAAIVQDDAVY
CCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
NTMYVQPLLWGVANGIELTQRPDNFFILRWVTVGAEN
HHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]