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The map label for this gene is hisF

Identifier: 89055286

GI number: 89055286

Start: 2810884

End: 2811669

Strand: Direct

Name: hisF

Synonym: Jann_2795

Alternate gene names: 89055286

Gene position: 2810884-2811669 (Clockwise)

Preceding gene: 89055284

Following gene: 89055287

Centisome position: 65.1

GC content: 63.36

Gene sequence:

>786_bases
ATGTTGAAGACCCGCATCATCCCCTGCCTCGACGTCGCCGAGGGCCGTACCGTCAAAGGCGTCAACTTTGTCGATCTGAT
CGACGCGGGCGATCCCGTTGAACAGGCCCGCGCCTATGACCTTGCGGGCGCGGATGAGCTTTGTTTTCTGGACATCAAGG
CCACCCATGAAAATCGCGGCACCATGTATGATCTGGCCACCCGCACGGCCGAGCAATGTTTCATGCCGCTGACCATCGGC
GGCGGCGTGCGCACGGTCGATGACGTGCGCAACCTGCTTCTGGCCGGGGCCGACAAGGTGTCGTTCAACTCCGCCGCCGT
GGCTGACCCTGACTGCGTGGCACGGGCGGCGGACAAGTTCGGCTCGCAATGTATCGTTGTGGCCATCGACGCCAAAACAG
TCAGGCCTCCCGCGCCCCTCCGGGGCGACCCCGGAAGGTGGGAGATCTTCACCCATGGCGGGCGCAAACCCACGGGCATT
GATGCTGTGGAATTCGCTAAGACCGTCGTGGCCAAGGGTGCCGGTGAAATCCTGCTGACCTCCATGGATCGCGACGGCAC
AAAGGCGGGCTTCAATCTACCGCTGACCAGGGCCGTGTCTGACGCCGTACCTGTGCCTGTCATCGCATCCGGCGGTGTCG
GCACGCTGGATCACCTGGTTGAAGGCGTCACCCAGGGCGGTGCGTCCGCCGTGCTGGCAGCCTCCATCTTCCACTTCGGC
ACCTATACGATCGGCGAGGCCAAGGCGCATATGGCCAAGGCCGGAATTCCTGTGAGGCTGACATGA

Upstream 100 bases:

>100_bases
CCTTTCGGGTCCGTCATTTAGAGCGGTCAGCTAACGCCTGCCGCGGCGCATATTCCAATCACTCTTTCGCGCGCAGCCGT
TTCAGCCTAAACCCGCCCAC

Downstream 100 bases:

>100_bases
CCCCTCTTCAACAGCTGGCCGCCACGATTGAAGCCCGCAAAGGGGCCGATCCAGCGTCGTCTTGGACGGCAAAACTTCTG
TCCAAAGGCCCTGAGAAATG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MLKTRIIPCLDVAEGRTVKGVNFVDLIDAGDPVEQARAYDLAGADELCFLDIKATHENRGTMYDLATRTAEQCFMPLTIG
GGVRTVDDVRNLLLAGADKVSFNSAAVADPDCVARAADKFGSQCIVVAIDAKTVRPPAPLRGDPGRWEIFTHGGRKPTGI
DAVEFAKTVVAKGAGEILLTSMDRDGTKAGFNLPLTRAVSDAVPVPVIASGGVGTLDHLVEGVTQGGASAVLAASIFHFG
TYTIGEAKAHMAKAGIPVRLT

Sequences:

>Translated_261_residues
MLKTRIIPCLDVAEGRTVKGVNFVDLIDAGDPVEQARAYDLAGADELCFLDIKATHENRGTMYDLATRTAEQCFMPLTIG
GGVRTVDDVRNLLLAGADKVSFNSAAVADPDCVARAADKFGSQCIVVAIDAKTVRPPAPLRGDPGRWEIFTHGGRKPTGI
DAVEFAKTVVAKGAGEILLTSMDRDGTKAGFNLPLTRAVSDAVPVPVIASGGVGTLDHLVEGVTQGGASAVLAASIFHFG
TYTIGEAKAHMAKAGIPVRLT
>Mature_261_residues
MLKTRIIPCLDVAEGRTVKGVNFVDLIDAGDPVEQARAYDLAGADELCFLDIKATHENRGTMYDLATRTAEQCFMPLTIG
GGVRTVDDVRNLLLAGADKVSFNSAAVADPDCVARAADKFGSQCIVVAIDAKTVRPPAPLRGDPGRWEIFTHGGRKPTGI
DAVEFAKTVVAKGAGEILLTSMDRDGTKAGFNLPLTRAVSDAVPVPVIASGGVGTLDHLVEGVTQGGASAVLAASIFHFG
TYTIGEAKAHMAKAGIPVRLT

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=264, Percent_Identity=39.0151515151515, Blast_Score=192, Evalue=1e-50,
Organism=Escherichia coli, GI87082028, Length=253, Percent_Identity=28.4584980237154, Blast_Score=87, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=30.7936507936508, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_JANSC (Q28NK0)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_510737.1
- HSSP:   P16250
- ProteinModelPortal:   Q28NK0
- SMR:   Q28NK0
- STRING:   Q28NK0
- GeneID:   3935260
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_2795
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   Q28NK0
- ProtClustDB:   PRK02083
- BioCyc:   JSP290400:JANN_2795-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27258; Mature: 27258

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTRIIPCLDVAEGRTVKGVNFVDLIDAGDPVEQARAYDLAGADELCFLDIKATHENRG
CCCCCCCCEEECCCCCEECCCCEEEEECCCCCHHHHHHCCCCCCCCEEEEEEEECCCCCC
TMYDLATRTAEQCFMPLTIGGGVRTVDDVRNLLLAGADKVSFNSAAVADPDCVARAADKF
CEEHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHC
GSQCIVVAIDAKTVRPPAPLRGDPGRWEIFTHGGRKPTGIDAVEFAKTVVAKGAGEILLT
CCEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEE
SMDRDGTKAGFNLPLTRAVSDAVPVPVIASGGVGTLDHLVEGVTQGGASAVLAASIFHFG
ECCCCCCCCCCCCCHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHC
TYTIGEAKAHMAKAGIPVRLT
CEEECHHHHHHHHCCCCEEEC
>Mature Secondary Structure
MLKTRIIPCLDVAEGRTVKGVNFVDLIDAGDPVEQARAYDLAGADELCFLDIKATHENRG
CCCCCCCCEEECCCCCEECCCCEEEEECCCCCHHHHHHCCCCCCCCEEEEEEEECCCCCC
TMYDLATRTAEQCFMPLTIGGGVRTVDDVRNLLLAGADKVSFNSAAVADPDCVARAADKF
CEEHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHC
GSQCIVVAIDAKTVRPPAPLRGDPGRWEIFTHGGRKPTGIDAVEFAKTVVAKGAGEILLT
CCEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEE
SMDRDGTKAGFNLPLTRAVSDAVPVPVIASGGVGTLDHLVEGVTQGGASAVLAASIFHFG
ECCCCCCCCCCCCCHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHC
TYTIGEAKAHMAKAGIPVRLT
CEEECHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA