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The map label for this gene is oprF [H]

Identifier: 89055277

GI number: 89055277

Start: 2803977

End: 2805422

Strand: Reverse

Name: oprF [H]

Synonym: Jann_2786

Alternate gene names: 89055277

Gene position: 2805422-2803977 (Counterclockwise)

Preceding gene: 89055278

Following gene: 89055276

Centisome position: 64.97

GC content: 65.08

Gene sequence:

>1446_bases
ATGCGTTACGCTCTACCGACCCTCGCCGTCGCCAGTTTTTGTCTTGCCGCCCATGGCGCTTTGGCACAGTCGACGCCCCC
GGGAACCTCGTTGACGGTTACCGACAGCCGCGGTGCTGACATCGTGTTTCCGCTTGGACTTGCCTCCTTCGCGGATGCGG
TGATCAGCTACGCGCCGGGCAGTGATATCCCGAGCGAGGCCGCCCGCAATCCCGCGCAGGTGCTGGGCTTTCCGGATTAC
GCGGGTGGCGATCAGGGTACGTTTGTGACCCTGGGTTGCGGCGGACAGGCGGTCTGGGCGTTCACCGACAACGCGCTGGT
CGATGTCGACGGCCCGGACCTTTGGATATTCGAAGTTGGGCCAGACGTGGAGGGGATGGCGGTGGCCGTGTCCGCCGATG
GGACCACATGGCTAGACGTGGGGAGTGTTGCGGGGTCAACCTCAACGGTGGATCTGGCCGGATATGCGCCCGCCGGGGCC
AGCTATCGCTTCGTGCGCCTGACCGATGATGGGGTGCAATGCTCGGGCCGCACGCCGGGCGCGGATGTGGATGCGATCGG
TGCCATCGGCGCGATTGAGCGGATCGCCTTGGATCTGCCCCCGACCTCTGACACGACGGCCTATGATGATACGCGTGGCA
GTCAGGTGCAGTTTCCGGCAGGCGATATCAGCTTTGCCGATGCCGTTGTCAGCTACACGCCCGGATCGACCATTGAAGCG
CCAGGCGTTGATGACCCGTCCCTTGGTCTCCATGCTCCGGACTATGTTGGCAATGGCGTACCTCTGGCGGTGACCCTGGG
CTGCGGCGGGCAGGTCGTGTACCAGTTCATCGACAACACCCTGATCGACGTGCCCGGCGCGGATCTTTTCATTTTCGAGG
TTGGTCCCGATGTGGAGGGCACGATCCTCGCCATTTCCGGCGATGGGGTCAGCTGGACGGATATCGGTGCCATCGAAGGG
GCGACGGCCCAGATCGACATCGCACCCCACATTCAACCCGGCGAGACCTTCCAGTTCGTGCGTCTGACCGATGGCGGCTT
GCAATGCAGCGGGCGGTTCCCGGGGGCCGACATTGATGCGGTGGGGGCAATCGGCTCTGCCACGCGGCTGGTGCTGGACG
CAAACCTGCTGTTTGATTTCGACAGCGCGACGCTGCGTCGCGCCGGTCTTCAGGCCGTGTCCGAATTGGCCGCTGCCATC
CGGTCGGGGCAGGTGTCGCAGGTCATCGTGGACGGGCACACCGATGCGGCAGGCTCGGACGCCTATAACCAGACCCTCTC
GCAGGAGCGCGCCGTTGCGGTGGCGCAGGCGCTGATGGTGCAGGGCGTGGCGCAAGGGCTGTTGACGACGCGGGCGTTCG
GAGAGAGCCGTCCGGTGGCCGACAATGGGTCGGAGGCCGGTGCACAGCAAAACCGCCGGGTCGAGGTTCTCTTGATCGGC
GGCTGA

Upstream 100 bases:

>100_bases
TGCAAGAGAGAATTTCCCGCCGTTGCGCCATTCGGTTCAGCACGGCAAGGTGAAGGACCCAGATGGTCTTACCCAGTCCC
TCCCGAACGGAGATATCTTC

Downstream 100 bases:

>100_bases
CGACGCGACAAATTTTAGACGTGGTGGGCAGCATATCGCGCGCGGACAGTTGAACGCCCAAGACAGCCCCATTTATGCGG
AGACATTTGAATGAGATTTG

Product: OmpA/MotB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MRYALPTLAVASFCLAAHGALAQSTPPGTSLTVTDSRGADIVFPLGLASFADAVISYAPGSDIPSEAARNPAQVLGFPDY
AGGDQGTFVTLGCGGQAVWAFTDNALVDVDGPDLWIFEVGPDVEGMAVAVSADGTTWLDVGSVAGSTSTVDLAGYAPAGA
SYRFVRLTDDGVQCSGRTPGADVDAIGAIGAIERIALDLPPTSDTTAYDDTRGSQVQFPAGDISFADAVVSYTPGSTIEA
PGVDDPSLGLHAPDYVGNGVPLAVTLGCGGQVVYQFIDNTLIDVPGADLFIFEVGPDVEGTILAISGDGVSWTDIGAIEG
ATAQIDIAPHIQPGETFQFVRLTDGGLQCSGRFPGADIDAVGAIGSATRLVLDANLLFDFDSATLRRAGLQAVSELAAAI
RSGQVSQVIVDGHTDAAGSDAYNQTLSQERAVAVAQALMVQGVAQGLLTTRAFGESRPVADNGSEAGAQQNRRVEVLLIG
G

Sequences:

>Translated_481_residues
MRYALPTLAVASFCLAAHGALAQSTPPGTSLTVTDSRGADIVFPLGLASFADAVISYAPGSDIPSEAARNPAQVLGFPDY
AGGDQGTFVTLGCGGQAVWAFTDNALVDVDGPDLWIFEVGPDVEGMAVAVSADGTTWLDVGSVAGSTSTVDLAGYAPAGA
SYRFVRLTDDGVQCSGRTPGADVDAIGAIGAIERIALDLPPTSDTTAYDDTRGSQVQFPAGDISFADAVVSYTPGSTIEA
PGVDDPSLGLHAPDYVGNGVPLAVTLGCGGQVVYQFIDNTLIDVPGADLFIFEVGPDVEGTILAISGDGVSWTDIGAIEG
ATAQIDIAPHIQPGETFQFVRLTDGGLQCSGRFPGADIDAVGAIGSATRLVLDANLLFDFDSATLRRAGLQAVSELAAAI
RSGQVSQVIVDGHTDAAGSDAYNQTLSQERAVAVAQALMVQGVAQGLLTTRAFGESRPVADNGSEAGAQQNRRVEVLLIG
G
>Mature_481_residues
MRYALPTLAVASFCLAAHGALAQSTPPGTSLTVTDSRGADIVFPLGLASFADAVISYAPGSDIPSEAARNPAQVLGFPDY
AGGDQGTFVTLGCGGQAVWAFTDNALVDVDGPDLWIFEVGPDVEGMAVAVSADGTTWLDVGSVAGSTSTVDLAGYAPAGA
SYRFVRLTDDGVQCSGRTPGADVDAIGAIGAIERIALDLPPTSDTTAYDDTRGSQVQFPAGDISFADAVVSYTPGSTIEA
PGVDDPSLGLHAPDYVGNGVPLAVTLGCGGQVVYQFIDNTLIDVPGADLFIFEVGPDVEGTILAISGDGVSWTDIGAIEG
ATAQIDIAPHIQPGETFQFVRLTDGGLQCSGRFPGADIDAVGAIGSATRLVLDANLLFDFDSATLRRAGLQAVSELAAAI
RSGQVSQVIVDGHTDAAGSDAYNQTLSQERAVAVAQALMVQGVAQGLLTTRAFGESRPVADNGSEAGAQQNRRVEVLLIG
G

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=109, Percent_Identity=39.4495412844037, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1787191, Length=124, Percent_Identity=33.8709677419355, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788957, Length=104, Percent_Identity=35.5769230769231, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 48942; Mature: 48942

Theoretical pI: Translated: 3.78; Mature: 3.78

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYALPTLAVASFCLAAHGALAQSTPPGTSLTVTDSRGADIVFPLGLASFADAVISYAPG
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEECCHHHHHHHHHCCCCC
SDIPSEAARNPAQVLGFPDYAGGDQGTFVTLGCGGQAVWAFTDNALVDVDGPDLWIFEVG
CCCCHHHHCCCHHEECCCCCCCCCCCCEEEEECCCEEEEEEECCEEEECCCCCEEEEEEC
PDVEGMAVAVSADGTTWLDVGSVAGSTSTVDLAGYAPAGASYRFVRLTDDGVQCSGRTPG
CCCCCEEEEEECCCCEEEEECCCCCCCCEEEEECCCCCCCCEEEEEEECCCCEECCCCCC
ADVDAIGAIGAIERIALDLPPTSDTTAYDDTRGSQVQFPAGDISFADAVVSYTPGSTIEA
CCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCHHCEEEEECCCCEEEC
PGVDDPSLGLHAPDYVGNGVPLAVTLGCGGQVVYQFIDNTLIDVPGADLFIFEVGPDVEG
CCCCCCCCCCCCCHHCCCCCEEEEEECCCHHHHHHHHCCCEEECCCCCEEEEEECCCCCE
TILAISGDGVSWTDIGAIEGATAQIDIAPHIQPGETFQFVRLTDGGLQCSGRFPGADIDA
EEEEEECCCCCEEECCCCCCCEEEEEECCCCCCCCCEEEEEEECCCEEECCCCCCCCCHH
VGAIGSATRLVLDANLLFDFDSATLRRAGLQAVSELAAAIRSGQVSQVIVDGHTDAAGSD
HCCCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCC
AYNQTLSQERAVAVAQALMVQGVAQGLLTTRAFGESRPVADNGSEAGAQQNRRVEVLLIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEC
G
C
>Mature Secondary Structure
MRYALPTLAVASFCLAAHGALAQSTPPGTSLTVTDSRGADIVFPLGLASFADAVISYAPG
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEECCHHHHHHHHHCCCCC
SDIPSEAARNPAQVLGFPDYAGGDQGTFVTLGCGGQAVWAFTDNALVDVDGPDLWIFEVG
CCCCHHHHCCCHHEECCCCCCCCCCCCEEEEECCCEEEEEEECCEEEECCCCCEEEEEEC
PDVEGMAVAVSADGTTWLDVGSVAGSTSTVDLAGYAPAGASYRFVRLTDDGVQCSGRTPG
CCCCCEEEEEECCCCEEEEECCCCCCCCEEEEECCCCCCCCEEEEEEECCCCEECCCCCC
ADVDAIGAIGAIERIALDLPPTSDTTAYDDTRGSQVQFPAGDISFADAVVSYTPGSTIEA
CCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCHHCEEEEECCCCEEEC
PGVDDPSLGLHAPDYVGNGVPLAVTLGCGGQVVYQFIDNTLIDVPGADLFIFEVGPDVEG
CCCCCCCCCCCCCHHCCCCCEEEEEECCCHHHHHHHHCCCEEECCCCCEEEEEECCCCCE
TILAISGDGVSWTDIGAIEGATAQIDIAPHIQPGETFQFVRLTDGGLQCSGRFPGADIDA
EEEEEECCCCCEEECCCCCCCEEEEEECCCCCCCCCEEEEEEECCCEEECCCCCCCCCHH
VGAIGSATRLVLDANLLFDFDSATLRRAGLQAVSELAAAIRSGQVSQVIVDGHTDAAGSD
HCCCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCC
AYNQTLSQERAVAVAQALMVQGVAQGLLTTRAFGESRPVADNGSEAGAQQNRRVEVLLIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]