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The map label for this gene is murE

Identifier: 89055257

GI number: 89055257

Start: 2781362

End: 2782834

Strand: Reverse

Name: murE

Synonym: Jann_2766

Alternate gene names: 89055257

Gene position: 2782834-2781362 (Counterclockwise)

Preceding gene: 89055258

Following gene: 89055256

Centisome position: 64.45

GC content: 62.25

Gene sequence:

>1473_bases
ATGGCGAAACGACTATCCGATCTTGGGCTGACACCGAAATCGGGCGGCGACGTGGTGGTCTCGGACCTGGCCTTCGATAG
TCGTAAGGTCCAGGCGGGCACCCTGTTTGCGGCTTTGCCCGGCACGCGGGTGCATGGTGGCGACTTTATCCAATATGCGT
TGCGTATGGGCGCGGGCGCCATCTTGACCGACCGCGCCGGTGCGGAGATTGCAGCGACAGATCTGGCCGCTACCGACGCG
GCCCTCGTCGTCGTGGAGGATGCGGCGGACGCTCTGGCCCATGCCGCCGCGCTATTTTTTGGTGCGCAGCCCGAGACGAT
GGTCGCTGTGACGGGAACGAATGGGAAGACGTCCGTCGCCAGTTTCACGCGTCAGATCTGGGAAGCGCTTGGCGTGCCCG
CCGTGAACATCGGCACCACGGGCGTTGAGGGCGCATTTGAAGCACCCGGCATCCATACTACGCCGGAGCCCGTCACTCTG
CACCGGGTTCTGGCAGAGATGGGGGCGGATGGCGTGACCCATGCGGCGATGGAAGCGTCATCCCACGGGCTGGACCAAAA
GCGCATGGACGGCGTGCGGCTTGCCGCGGCGGCATTTACCAACCTGACCCAGGATCATCTGGACTATCACGGAACCATGG
AGGCCTATTTTGAGGCCAAACTGCGCTTGTTCACCGGGCTTCTGCCCGATGGTGGCGTAGCTGTGGTGAACCTTGACGAC
GATCACGGGCTTCGCGTTGCAGAGGCATGTTCCACCCGGGGGATCGAGGTGATCGGCGTTGCGCAATATGCCGAGGCCGC
GCTGAAAATTACGGGTCGGCGCATTGATGAGACGGGCCAAGACGTCCTCTTCCGGTGGCAGGGAGACGCTTGCCAGGTGC
GCCTGAACCTGATCGGCGCGTTCCAGGCGATGAATGTCCTCACAGCCGCCGGTCTGGTGATCGCGGGCGGCGAAGACCCA
GAGGACGTTTTTGCAGTTCTCCACAAGATGGAGGGTGTACGGGGCCGGATGCAGTTGGCCGCGCGGCGCAGTCATGGGGC
CCCCATCTATGTGGATTATTCGCATACTCCTGATAGTCTAGAAGTCGCACTCAAAGCATTGCGCCCCCACGTTCTGGGCC
GCCTTGTCGTTGTTTTCGGTGCTGGAGGGGATCGTGATACGGCAAAAAGACCTCTCATGGGCAACGCAGCCACCCTGTGG
AGTGATGTTGCATTTGTGACAGATGATAATCCACGCACCGAAGATCCCGCAGCCATCCGTGCGGCTGTTTTGGCCGGTTG
TCCGGAGGCCACGGTCGTCGCGGATCGGGCGGAGGCAATCCTACGGGCTGTGGATATGTTGGAGCCCGGCGATGCGCTTC
TCATAGCCGGAAAGGGCCATGAGACGGGCCAGCAAGTGGGCGATGTCATCTACCCGTTTGACGATGTCGAACAGGCGTCG
GTCGCGGTTGCCGCCCTGGAGGGCGCGATATGA

Upstream 100 bases:

>100_bases
GATCATCCGCCGCACCGCGCCGCTTCTGGGCCTACGTCCAGACGTTGAACATCCCCTCGGTTTGCAGCTATCCAACTCCT
CTAACTAAGGGGTGGTGCAG

Downstream 100 bases:

>100_bases
TTTTGTGGACTTCGGCAGACGCGGCATCGGCCACGGGCGGCACAAGTCAGGGCACCTGGACCGCGACGGGTGTGTCCATC
GACACCCGCACGATTGAGCC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MAKRLSDLGLTPKSGGDVVVSDLAFDSRKVQAGTLFAALPGTRVHGGDFIQYALRMGAGAILTDRAGAEIAATDLAATDA
ALVVVEDAADALAHAAALFFGAQPETMVAVTGTNGKTSVASFTRQIWEALGVPAVNIGTTGVEGAFEAPGIHTTPEPVTL
HRVLAEMGADGVTHAAMEASSHGLDQKRMDGVRLAAAAFTNLTQDHLDYHGTMEAYFEAKLRLFTGLLPDGGVAVVNLDD
DHGLRVAEACSTRGIEVIGVAQYAEAALKITGRRIDETGQDVLFRWQGDACQVRLNLIGAFQAMNVLTAAGLVIAGGEDP
EDVFAVLHKMEGVRGRMQLAARRSHGAPIYVDYSHTPDSLEVALKALRPHVLGRLVVVFGAGGDRDTAKRPLMGNAATLW
SDVAFVTDDNPRTEDPAAIRAAVLAGCPEATVVADRAEAILRAVDMLEPGDALLIAGKGHETGQQVGDVIYPFDDVEQAS
VAVAALEGAI

Sequences:

>Translated_490_residues
MAKRLSDLGLTPKSGGDVVVSDLAFDSRKVQAGTLFAALPGTRVHGGDFIQYALRMGAGAILTDRAGAEIAATDLAATDA
ALVVVEDAADALAHAAALFFGAQPETMVAVTGTNGKTSVASFTRQIWEALGVPAVNIGTTGVEGAFEAPGIHTTPEPVTL
HRVLAEMGADGVTHAAMEASSHGLDQKRMDGVRLAAAAFTNLTQDHLDYHGTMEAYFEAKLRLFTGLLPDGGVAVVNLDD
DHGLRVAEACSTRGIEVIGVAQYAEAALKITGRRIDETGQDVLFRWQGDACQVRLNLIGAFQAMNVLTAAGLVIAGGEDP
EDVFAVLHKMEGVRGRMQLAARRSHGAPIYVDYSHTPDSLEVALKALRPHVLGRLVVVFGAGGDRDTAKRPLMGNAATLW
SDVAFVTDDNPRTEDPAAIRAAVLAGCPEATVVADRAEAILRAVDMLEPGDALLIAGKGHETGQQVGDVIYPFDDVEQAS
VAVAALEGAI
>Mature_489_residues
AKRLSDLGLTPKSGGDVVVSDLAFDSRKVQAGTLFAALPGTRVHGGDFIQYALRMGAGAILTDRAGAEIAATDLAATDAA
LVVVEDAADALAHAAALFFGAQPETMVAVTGTNGKTSVASFTRQIWEALGVPAVNIGTTGVEGAFEAPGIHTTPEPVTLH
RVLAEMGADGVTHAAMEASSHGLDQKRMDGVRLAAAAFTNLTQDHLDYHGTMEAYFEAKLRLFTGLLPDGGVAVVNLDDD
HGLRVAEACSTRGIEVIGVAQYAEAALKITGRRIDETGQDVLFRWQGDACQVRLNLIGAFQAMNVLTAAGLVIAGGEDPE
DVFAVLHKMEGVRGRMQLAARRSHGAPIYVDYSHTPDSLEVALKALRPHVLGRLVVVFGAGGDRDTAKRPLMGNAATLWS
DVAFVTDDNPRTEDPAAIRAAVLAGCPEATVVADRAEAILRAVDMLEPGDALLIAGKGHETGQQVGDVIYPFDDVEQASV
AVAALEGAI

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=486, Percent_Identity=37.2427983539095, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1786274, Length=253, Percent_Identity=28.4584980237154, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 51167; Mature: 51036

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRLSDLGLTPKSGGDVVVSDLAFDSRKVQAGTLFAALPGTRVHGGDFIQYALRMGAGA
CCCCCHHCCCCCCCCCCEEEEEECCCCCEEECCEEEEECCCCCCCCHHHHHHHHHHCCCE
ILTDRAGAEIAATDLAATDAALVVVEDAADALAHAAALFFGAQPETMVAVTGTNGKTSVA
EEECCCCCEEEEHHHCCCCEEEEEEECHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHH
SFTRQIWEALGVPAVNIGTTGVEGAFEAPGIHTTPEPVTLHRVLAEMGADGVTHAAMEAS
HHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
SHGLDQKRMDGVRLAAAAFTNLTQDHLDYHGTMEAYFEAKLRLFTGLLPDGGVAVVNLDD
HCCCCHHHHCCHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECC
DHGLRVAEACSTRGIEVIGVAQYAEAALKITGRRIDETGQDVLFRWQGDACQVRLNLIGA
CCCCCHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEEHHHHH
FQAMNVLTAAGLVIAGGEDPEDVFAVLHKMEGVRGRMQLAARRSHGAPIYVDYSHTPDSL
HHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEEEECCCCCHHH
EVALKALRPHVLGRLVVVFGAGGDRDTAKRPLMGNAATLWSDVAFVTDDNPRTEDPAAIR
HHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCHHHHHHCEEEEECCCCCCCCHHHHH
AAVLAGCPEATVVADRAEAILRAVDMLEPGDALLIAGKGHETGQQVGDVIYPFDDVEQAS
HHHHHCCCCHHEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCEECCCCCHHHHH
VAVAALEGAI
HHHHHHCCCC
>Mature Secondary Structure 
AKRLSDLGLTPKSGGDVVVSDLAFDSRKVQAGTLFAALPGTRVHGGDFIQYALRMGAGA
CCCCHHCCCCCCCCCCEEEEEECCCCCEEECCEEEEECCCCCCCCHHHHHHHHHHCCCE
ILTDRAGAEIAATDLAATDAALVVVEDAADALAHAAALFFGAQPETMVAVTGTNGKTSVA
EEECCCCCEEEEHHHCCCCEEEEEEECHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHH
SFTRQIWEALGVPAVNIGTTGVEGAFEAPGIHTTPEPVTLHRVLAEMGADGVTHAAMEAS
HHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
SHGLDQKRMDGVRLAAAAFTNLTQDHLDYHGTMEAYFEAKLRLFTGLLPDGGVAVVNLDD
HCCCCHHHHCCHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECC
DHGLRVAEACSTRGIEVIGVAQYAEAALKITGRRIDETGQDVLFRWQGDACQVRLNLIGA
CCCCCHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEEHHHHH
FQAMNVLTAAGLVIAGGEDPEDVFAVLHKMEGVRGRMQLAARRSHGAPIYVDYSHTPDSL
HHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEEEECCCCCHHH
EVALKALRPHVLGRLVVVFGAGGDRDTAKRPLMGNAATLWSDVAFVTDDNPRTEDPAAIR
HHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCHHHHHHCEEEEECCCCCCCCHHHHH
AAVLAGCPEATVVADRAEAILRAVDMLEPGDALLIAGKGHETGQQVGDVIYPFDDVEQAS
HHHHHCCCCHHEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCEECCCCCHHHHH
VAVAALEGAI
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]