Click here to switch to the map view.

The map label for this gene is mdeA [H]

Identifier: 89055212

GI number: 89055212

Start: 2728385

End: 2729677

Strand: Direct

Name: mdeA [H]

Synonym: Jann_2721

Alternate gene names: 89055212

Gene position: 2728385-2729677 (Clockwise)

Preceding gene: 89055208

Following gene: 89055213

Centisome position: 63.19

GC content: 62.57

Gene sequence:

>1293_bases
ATGACAGACGGCCCCAGCTACGGCTTCGACACTCTACAGATCCACGCAGGCGCGCGGCCTGACCCTGCAACAGGCGCACG
GCAGACGCCGATCTACCAGACCACGGCCTATGTGTTCCGCGACGCCGATCACGCAGCGGCTTTGTTCAACCTGCAGGAAG
TCGGCTACATCTATTCGCGCCTGACCAACCCGACAGTTGCCGTGTTGCAGGAACGGATCGCGACACTGGAAGGCGGCGCG
GGCGCGGTGTGCTGTTCATCGGGCCACGCCGCACAGATCATGGCGTTGTTCCCGCTGATGGCGCCGGGGCGCAATGTGGT
TGTGTCCACGCGCCTTTATGGCGGCACGGTCACGCAGTTCAGCCAAACGATCAAGCGCTTCGGGTGGGAGGCGAAGTTCG
TCGATATGGACGATCTGGACGCGGTGAAGGCCGCCATCGACGACAACACACGCGCGATCTTTGGTGAGGCCATCGCCAAC
CCCGGCGGGTACATCATGGATGTGCGCGCCGTGGCCGATATCGCCGATGAGGCGGGCATCCCGCTGATCATCGACAACAC
CACGGCCACGCCTTACCTGCACCGTCCGATTGAGCATGGGGCCACGCTTGTTGTGCATTCCACGACCAAATACCTCACCG
GCAATGGCACCGTCACCGGCGGCTGCGTCGTGGATTCGGGCAAATTCGACTGGAGCGCATCGGATAAGTTCCCCTCGCTC
TCGCAGCCCGAGCCCGCCTATCACGGCCTGAAATTCGCCGAGACGTTCGGGCCGCTGGCCTACACGTTCCACGGCATCGC
CATCGGGTTGCGCGATCTGGGCATGACGATGAACCCGCAAGCGGCGCATTATACGCTGATGGGGATCGAGACGTTGTCGC
TGCGGATGGAGCGGCATGTGGAGAATGCCCAAAAGATCGCGAAATGGCTGGAGGATGACCCGCGCATCGACTTCGTGACC
TATGCAGGGTTGGAAAGCAGTCCGTATTTCCACCGTGTGAAGGACGTCTGCCCCAAAGGCGCGGGCGCGCTGTTCACCTT
TGCGGTGAAGGGCGGTTATGACGCCTGTATCAAGCTGGTCGATTCGCTGGAGATCTTCAGCCACGTGGCCAATTTGGGCG
ATACCCGCTCGCTGATCATCCATTCGGCCTCCACCACCCACCGCCAGCTGACGCCCGAGCAGCAGGAGGCGGCAGGCGCG
GCCCCCAATGTGGTGCGGGTGTCCATCGGCACCGAAGATGTGAACGACCTTATCGCAGATCTGGACCGGGCCCTGTCCGC
CGCAACGTCGTAA

Upstream 100 bases:

>100_bases
ACGGCACGTTTTTTAGAACAGGCGGCCTTCGATTGCGGCGGGTTGTTGCGGGTCGTGGGCCGCATGGCCTACAACCGGGC
AAATTATCCGGAGAAAGACC

Downstream 100 bases:

>100_bases
GGGGGAATGGGGTCCCGGAAGGTTGGGATTTCATTAACCAATTTTACTCTAGACTATGTGGGCGGCGCGGAGGTCATTCG
CGCCGCCCCCTTTCTTTGGG

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTDGPSYGFDTLQIHAGARPDPATGARQTPIYQTTAYVFRDADHAAALFNLQEVGYIYSRLTNPTVAVLQERIATLEGGA
GAVCCSSGHAAQIMALFPLMAPGRNVVVSTRLYGGTVTQFSQTIKRFGWEAKFVDMDDLDAVKAAIDDNTRAIFGEAIAN
PGGYIMDVRAVADIADEAGIPLIIDNTTATPYLHRPIEHGATLVVHSTTKYLTGNGTVTGGCVVDSGKFDWSASDKFPSL
SQPEPAYHGLKFAETFGPLAYTFHGIAIGLRDLGMTMNPQAAHYTLMGIETLSLRMERHVENAQKIAKWLEDDPRIDFVT
YAGLESSPYFHRVKDVCPKGAGALFTFAVKGGYDACIKLVDSLEIFSHVANLGDTRSLIIHSASTTHRQLTPEQQEAAGA
APNVVRVSIGTEDVNDLIADLDRALSAATS

Sequences:

>Translated_430_residues
MTDGPSYGFDTLQIHAGARPDPATGARQTPIYQTTAYVFRDADHAAALFNLQEVGYIYSRLTNPTVAVLQERIATLEGGA
GAVCCSSGHAAQIMALFPLMAPGRNVVVSTRLYGGTVTQFSQTIKRFGWEAKFVDMDDLDAVKAAIDDNTRAIFGEAIAN
PGGYIMDVRAVADIADEAGIPLIIDNTTATPYLHRPIEHGATLVVHSTTKYLTGNGTVTGGCVVDSGKFDWSASDKFPSL
SQPEPAYHGLKFAETFGPLAYTFHGIAIGLRDLGMTMNPQAAHYTLMGIETLSLRMERHVENAQKIAKWLEDDPRIDFVT
YAGLESSPYFHRVKDVCPKGAGALFTFAVKGGYDACIKLVDSLEIFSHVANLGDTRSLIIHSASTTHRQLTPEQQEAAGA
APNVVRVSIGTEDVNDLIADLDRALSAATS
>Mature_429_residues
TDGPSYGFDTLQIHAGARPDPATGARQTPIYQTTAYVFRDADHAAALFNLQEVGYIYSRLTNPTVAVLQERIATLEGGAG
AVCCSSGHAAQIMALFPLMAPGRNVVVSTRLYGGTVTQFSQTIKRFGWEAKFVDMDDLDAVKAAIDDNTRAIFGEAIANP
GGYIMDVRAVADIADEAGIPLIIDNTTATPYLHRPIEHGATLVVHSTTKYLTGNGTVTGGCVVDSGKFDWSASDKFPSLS
QPEPAYHGLKFAETFGPLAYTFHGIAIGLRDLGMTMNPQAAHYTLMGIETLSLRMERHVENAQKIAKWLEDDPRIDFVTY
AGLESSPYFHRVKDVCPKGAGALFTFAVKGGYDACIKLVDSLEIFSHVANLGDTRSLIIHSASTTHRQLTPEQQEAAGAA
PNVVRVSIGTEDVNDLIADLDRALSAATS

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=422, Percent_Identity=30.0947867298578, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI299473759, Length=422, Percent_Identity=28.1990521327014, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI25453487, Length=421, Percent_Identity=27.0783847980998, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI1790375, Length=420, Percent_Identity=30.7142857142857, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1789383, Length=423, Percent_Identity=26.0047281323877, Blast_Score=108, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71980708, Length=414, Percent_Identity=32.1256038647343, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17538055, Length=423, Percent_Identity=31.2056737588652, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17560056, Length=432, Percent_Identity=31.4814814814815, Blast_Score=159, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=44.367816091954, Blast_Score=334, Evalue=2e-92,
Organism=Saccharomyces cerevisiae, GI6319307, Length=438, Percent_Identity=31.0502283105023, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6321170, Length=368, Percent_Identity=24.1847826086957, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI19922554, Length=425, Percent_Identity=31.0588235294118, Blast_Score=188, Evalue=8e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46203; Mature: 46071

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00196 COPPER_BLUE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDGPSYGFDTLQIHAGARPDPATGARQTPIYQTTAYVFRDADHAAALFNLQEVGYIYSR
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHH
LTNPTVAVLQERIATLEGGAGAVCCSSGHAAQIMALFPLMAPGRNVVVSTRLYGGTVTQF
HCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCEEEEEEECCCHHHHH
SQTIKRFGWEAKFVDMDDLDAVKAAIDDNTRAIFGEAIANPGGYIMDVRAVADIADEAGI
HHHHHHHCCCEEEECCCCHHHHHHHHCCCCCEEEHHHHCCCCCEEEEHHHHHHHHHHCCC
PLIIDNTTATPYLHRPIEHGATLVVHSTTKYLTGNGTVTGGCVVDSGKFDWSASDKFPSL
EEEEECCCCCCCCCCHHHCCCEEEEECCEEEEECCCEEECCEEEECCCCCCCCCCCCCCC
SQPEPAYHGLKFAETFGPLAYTFHGIAIGLRDLGMTMNPQAAHYTLMGIETLSLRMERHV
CCCCCHHCCCCHHHHHCCHHHHHHHHEEEHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHH
ENAQKIAKWLEDDPRIDFVTYAGLESSPYFHRVKDVCPKGAGALFTFAVKGGYDACIKLV
HHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHH
DSLEIFSHVANLGDTRSLIIHSASTTHRQLTPEQQEAAGAAPNVVRVSIGTEDVNDLIAD
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCEEEEEECCHHHHHHHHH
LDRALSAATS
HHHHHHHCCC
>Mature Secondary Structure 
TDGPSYGFDTLQIHAGARPDPATGARQTPIYQTTAYVFRDADHAAALFNLQEVGYIYSR
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHH
LTNPTVAVLQERIATLEGGAGAVCCSSGHAAQIMALFPLMAPGRNVVVSTRLYGGTVTQF
HCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCEEEEEEECCCHHHHH
SQTIKRFGWEAKFVDMDDLDAVKAAIDDNTRAIFGEAIANPGGYIMDVRAVADIADEAGI
HHHHHHHCCCEEEECCCCHHHHHHHHCCCCCEEEHHHHCCCCCEEEEHHHHHHHHHHCCC
PLIIDNTTATPYLHRPIEHGATLVVHSTTKYLTGNGTVTGGCVVDSGKFDWSASDKFPSL
EEEEECCCCCCCCCCHHHCCCEEEEECCEEEEECCCEEECCEEEECCCCCCCCCCCCCCC
SQPEPAYHGLKFAETFGPLAYTFHGIAIGLRDLGMTMNPQAAHYTLMGIETLSLRMERHV
CCCCCHHCCCCHHHHHCCHHHHHHHHEEEHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHH
ENAQKIAKWLEDDPRIDFVTYAGLESSPYFHRVKDVCPKGAGALFTFAVKGGYDACIKLV
HHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHH
DSLEIFSHVANLGDTRSLIIHSASTTHRQLTPEQQEAAGAAPNVVRVSIGTEDVNDLIAD
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCEEEEEECCHHHHHHHHH
LDRALSAATS
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]