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The map label for this gene is purB [H]

Identifier: 89055154

GI number: 89055154

Start: 2672986

End: 2674290

Strand: Direct

Name: purB [H]

Synonym: Jann_2663

Alternate gene names: 89055154

Gene position: 2672986-2674290 (Clockwise)

Preceding gene: 89055151

Following gene: 89055155

Centisome position: 61.9

GC content: 61.46

Gene sequence:

>1305_bases
ATGATCCCCCGCTATTCCCGCCCCGAAATGGTCGCCATTTGGGAACCCGCCACCAAGTTCCGCATCTGGTACGAGATCGA
GGCCCATGCCTGTGACGCGATGGCCGATCTGGGCGTGATCCCGCGTGAGAATGCCGAGGCCGTTTGGAAAGCCCGCGACG
TCGAATTTGACGTGGCCCGTATTGATGAGATCGAGGCCGTCACCAAGCATGACGTGATCGCCTTCCTTACCCATCTGGCC
GAACATGTGGGGGCGGAAGAAGCCCGTTTCGTCCATCAGGGCATGACGTCCTCGGACGTGCTGGACACCTGCTTCAACGT
CCAACTGGTGCGCGCGGCCGATACCCTTCTGGCGGATCTGGACAAGCTGCTGGCCGCGCTGAAACGGCGCGCGATGGAGC
ATAAGATGACCGTGCGCGTGGGCCGGTCCCACGGCATCCATGCAGAGCCCACGACAATGGGCCTGACGTTTGCGCGCTTC
TATGCGGAGATGGATCGCAATCGCACGCGCATGGCCATGGCCCGCGATGAGGTCGCAACCGGCGCGATCTCCGGTGCGGT
GGGGACATTCGCCAATATCGACCCGGCGGTGGAGGCCCATGTCTGCGAAAAACTTGGCCTGTCGCCGGAGCCGATTTCCA
CCCAGGTCATCCCCCGCGACCGCCACGCGATGTTCTTCGCAACCCTGGGCGTGATCGCATCGTCGATTGAAAACGTCGCC
ACGGAAATCCGCCATATGCAGCGCACGGAGGTGCTGGAGGGCGCGGAGTTCTTCTCCATCGGGCAGAAGGGCTCCTCCGC
GATGCCCCATAAGAAAAACCCGGTTCTGACGGAGAACCTGACCGGGCTGGCGCGTCTGGTGCGCATGTCTGTTGTGCCCG
CGATGGAGAATGTGGCGCTCTGGCATGAGCGGGATATCTCTCATTCGTCGGTGGAGCGCGCGATTGGGCCCGATGCCACG
GTGACCCTTGATTTCGCGTTGAACCGCCTGACGGGTGTCGTGGACAAGATGCTGATCTTCCCCGACAACATGATGGACAA
CATGAACAAATATCCCGGCCTGGTGATGTCCCAGCGGGTGCTTCTGGCCCTGACGCAGGCGGGCGTGTCGCGCGAGGATG
CATACGCCATGGTGCAGCGTAACGCTTTGAAAGTCTGGGAGGAGCGGACGGATTTTCGTGAGGAATTGCTGGCCGATACG
GAGGTTGTTGCAGCGCTTGGCGTCGACGGGATCGACGAGAAATTCGACCTCGGCTACCACACCAAACACGTCGATACGAT
CTTCGCGCGGGTTTTCGGAGACTGA

Upstream 100 bases:

>100_bases
TCACAACCGCCAGTCGTGTCATCGGGTTGCTCCCATCGCCCCAGGCCCGTATCAGCCGTTCCATATCAGACAGCGCCAAG
TTCTCGCGAAAGGCAAGCCC

Downstream 100 bases:

>100_bases
CCATCCCACGAGCTGCGCTAACGGATCCTTAATTATTTGGCCCTATCCCATCAGGACGTGATGAGAAAGGGTTCGCATGG
ACATGCTGCGCGATGCTGAC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MIPRYSRPEMVAIWEPATKFRIWYEIEAHACDAMADLGVIPRENAEAVWKARDVEFDVARIDEIEAVTKHDVIAFLTHLA
EHVGAEEARFVHQGMTSSDVLDTCFNVQLVRAADTLLADLDKLLAALKRRAMEHKMTVRVGRSHGIHAEPTTMGLTFARF
YAEMDRNRTRMAMARDEVATGAISGAVGTFANIDPAVEAHVCEKLGLSPEPISTQVIPRDRHAMFFATLGVIASSIENVA
TEIRHMQRTEVLEGAEFFSIGQKGSSAMPHKKNPVLTENLTGLARLVRMSVVPAMENVALWHERDISHSSVERAIGPDAT
VTLDFALNRLTGVVDKMLIFPDNMMDNMNKYPGLVMSQRVLLALTQAGVSREDAYAMVQRNALKVWEERTDFREELLADT
EVVAALGVDGIDEKFDLGYHTKHVDTIFARVFGD

Sequences:

>Translated_434_residues
MIPRYSRPEMVAIWEPATKFRIWYEIEAHACDAMADLGVIPRENAEAVWKARDVEFDVARIDEIEAVTKHDVIAFLTHLA
EHVGAEEARFVHQGMTSSDVLDTCFNVQLVRAADTLLADLDKLLAALKRRAMEHKMTVRVGRSHGIHAEPTTMGLTFARF
YAEMDRNRTRMAMARDEVATGAISGAVGTFANIDPAVEAHVCEKLGLSPEPISTQVIPRDRHAMFFATLGVIASSIENVA
TEIRHMQRTEVLEGAEFFSIGQKGSSAMPHKKNPVLTENLTGLARLVRMSVVPAMENVALWHERDISHSSVERAIGPDAT
VTLDFALNRLTGVVDKMLIFPDNMMDNMNKYPGLVMSQRVLLALTQAGVSREDAYAMVQRNALKVWEERTDFREELLADT
EVVAALGVDGIDEKFDLGYHTKHVDTIFARVFGD
>Mature_434_residues
MIPRYSRPEMVAIWEPATKFRIWYEIEAHACDAMADLGVIPRENAEAVWKARDVEFDVARIDEIEAVTKHDVIAFLTHLA
EHVGAEEARFVHQGMTSSDVLDTCFNVQLVRAADTLLADLDKLLAALKRRAMEHKMTVRVGRSHGIHAEPTTMGLTFARF
YAEMDRNRTRMAMARDEVATGAISGAVGTFANIDPAVEAHVCEKLGLSPEPISTQVIPRDRHAMFFATLGVIASSIENVA
TEIRHMQRTEVLEGAEFFSIGQKGSSAMPHKKNPVLTENLTGLARLVRMSVVPAMENVALWHERDISHSSVERAIGPDAT
VTLDFALNRLTGVVDKMLIFPDNMMDNMNKYPGLVMSQRVLLALTQAGVSREDAYAMVQRNALKVWEERTDFREELLADT
EVVAALGVDGIDEKFDLGYHTKHVDTIFARVFGD

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=388, Percent_Identity=26.2886597938144, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=26.509186351706, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI1787376, Length=348, Percent_Identity=27.2988505747126, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17508577, Length=416, Percent_Identity=26.6826923076923, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI32564234, Length=353, Percent_Identity=28.328611898017, Blast_Score=85, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6323391, Length=391, Percent_Identity=25.8312020460358, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24647570, Length=383, Percent_Identity=24.0208877284595, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48357; Mature: 48357

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYSRPEMVAIWEPATKFRIWYEIEAHACDAMADLGVIPRENAEAVWKARDVEFDVAR
CCCCCCCCCEEEEECCCCEEEEEEEEECHHHHHHHHCCCCCCCCCHHHHHHCCCEEHHHH
IDEIEAVTKHDVIAFLTHLAEHVGAEEARFVHQGMTSSDVLDTCFNVQLVRAADTLLADL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DKLLAALKRRAMEHKMTVRVGRSHGIHAEPTTMGLTFARFYAEMDRNRTRMAMARDEVAT
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
GAISGAVGTFANIDPAVEAHVCEKLGLSPEPISTQVIPRDRHAMFFATLGVIASSIENVA
HHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TEIRHMQRTEVLEGAEFFSIGQKGSSAMPHKKNPVLTENLTGLARLVRMSVVPAMENVAL
HHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERAIGPDATVTLDFALNRLTGVVDKMLIFPDNMMDNMNKYPGLVMSQRV
HHHCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHH
LLALTQAGVSREDAYAMVQRNALKVWEERTDFREELLADTEVVAALGVDGIDEKFDLGYH
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
TKHVDTIFARVFGD
HHHHHHHHHHHHCC
>Mature Secondary Structure
MIPRYSRPEMVAIWEPATKFRIWYEIEAHACDAMADLGVIPRENAEAVWKARDVEFDVAR
CCCCCCCCCEEEEECCCCEEEEEEEEECHHHHHHHHCCCCCCCCCHHHHHHCCCEEHHHH
IDEIEAVTKHDVIAFLTHLAEHVGAEEARFVHQGMTSSDVLDTCFNVQLVRAADTLLADL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DKLLAALKRRAMEHKMTVRVGRSHGIHAEPTTMGLTFARFYAEMDRNRTRMAMARDEVAT
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
GAISGAVGTFANIDPAVEAHVCEKLGLSPEPISTQVIPRDRHAMFFATLGVIASSIENVA
HHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TEIRHMQRTEVLEGAEFFSIGQKGSSAMPHKKNPVLTENLTGLARLVRMSVVPAMENVAL
HHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERAIGPDATVTLDFALNRLTGVVDKMLIFPDNMMDNMNKYPGLVMSQRV
HHHCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHH
LLALTQAGVSREDAYAMVQRNALKVWEERTDFREELLADTEVVAALGVDGIDEKFDLGYH
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
TKHVDTIFARVFGD
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]