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The map label for this gene is tgt

Identifier: 89055011

GI number: 89055011

Start: 2520139

End: 2521269

Strand: Reverse

Name: tgt

Synonym: Jann_2520

Alternate gene names: 89055011

Gene position: 2521269-2520139 (Counterclockwise)

Preceding gene: 89055013

Following gene: 89055010

Centisome position: 58.39

GC content: 64.37

Gene sequence:

>1131_bases
ATGACCAGAGCATCCTCTTTCCGCCTCGACGCAACCTCGGGCAAGGCCCGCACGGGTGTGATCCAAACCCCGCGCGGCGA
CATTCGCACGCCCGCCTTCATGCCCGTGGGCACGGCCGCCACGGTCAAGGCGATGATGCCTGAATCGGTGGCCGCGACCG
GCGCGGATATTCTGTTGGGCAACACCTATCACCTGATGCTGCGCCCCACGGCGGAGCGGATCGCCAACCTCGGCGGGCTG
CACAGCTTCATGAATTGGGACAAGCCGATCCTGACGGATTCGGGCGGGTTCCAAGTCATGAGCCTCGCCGATCTGCGCAA
GCTGACCGAGGAGGGCGTGACCTTCCGGTCCCACATCGACGGGTCCAAACATCTGCTCAGCCCCGAGCGGTCGATGGAGA
TCCAGAAGCTTCTCGGCTCGGACATTGTCATGTGTTTCGATGAATGCCCCGCGCTGCCCGCAGATCGCAAAGCGCTGGAC
GACAGCATGCGCCTGTCGATGCGGTGGGCCGCGCGGTCCAAGGAGGCGTTCGGGGACCGTCCCGGCTATGCGTTGTTCGG
CATCCAGCAGGGTGGTTTGGAAGAAGACCTGCGTGAGGAATCCGCCAAGGCCCTGCGGGAGATCGGGTTCGATGGCTACG
CGGTCGGCGGCCTTGCGGTGGGAGAGGGGCAGGCGGCCATGTTCGGCTGCCTCGACTTCGCGCCGGATCAACTTCCCACC
GACAAGCCGCGCTACCTGATGGGCGTGGGCAAGCCGGACGATATCGTGGGCGCGGTGAAGCGCGGCATAGACATGATGGA
TTGCGTGCTGCCATCTCGCTCGGGCCGCACCGGGCAGGCGTGGACGCGGCGGGGGCAGGTGAACATCAAGAATGCGCGCC
ACGCCGATGACCCCCGCCCGCTGGACGAGGACTGCACCTGTCCTGCGTGCCGCAACTATTCCCGCGCATATCTGCACCAT
GTGTTTCGCGCACAGGAGATGATCAGCGGTATGCTCCTGACCTGGCACAACCTGCACTATTATCAGGAGCTGATGCAGAC
GATGCGCGATGCCATCGCCGCGAATGACTTTGCAGGGTTTGAGGCGCGGTTTCACGCGGACCGTGCGGAAGGCGATATCG
TGCCACTCTAG

Upstream 100 bases:

>100_bases
AACAAGGTGATGTCGGTGCTTGGATTGCGCAGGGCCCGTGGCCGGGGGCGGCGTCGCTAGGTGCTTTGCATTTTGCCCGG
GACGCGGCTAAGCGGAGCGG

Downstream 100 bases:

>100_bases
TGTTCAGTTGAACAGGCGCATTTTGCCGATGAACCCTCGTTTTCCCCCCTCTTTCGCCCCGATCCTGCCGAAATCCCTGC
TTAGGTCCCAGAGGCTGATC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTRASSFRLDATSGKARTGVIQTPRGDIRTPAFMPVGTAATVKAMMPESVAATGADILLGNTYHLMLRPTAERIANLGGL
HSFMNWDKPILTDSGGFQVMSLADLRKLTEEGVTFRSHIDGSKHLLSPERSMEIQKLLGSDIVMCFDECPALPADRKALD
DSMRLSMRWAARSKEAFGDRPGYALFGIQQGGLEEDLREESAKALREIGFDGYAVGGLAVGEGQAAMFGCLDFAPDQLPT
DKPRYLMGVGKPDDIVGAVKRGIDMMDCVLPSRSGRTGQAWTRRGQVNIKNARHADDPRPLDEDCTCPACRNYSRAYLHH
VFRAQEMISGMLLTWHNLHYYQELMQTMRDAIAANDFAGFEARFHADRAEGDIVPL

Sequences:

>Translated_376_residues
MTRASSFRLDATSGKARTGVIQTPRGDIRTPAFMPVGTAATVKAMMPESVAATGADILLGNTYHLMLRPTAERIANLGGL
HSFMNWDKPILTDSGGFQVMSLADLRKLTEEGVTFRSHIDGSKHLLSPERSMEIQKLLGSDIVMCFDECPALPADRKALD
DSMRLSMRWAARSKEAFGDRPGYALFGIQQGGLEEDLREESAKALREIGFDGYAVGGLAVGEGQAAMFGCLDFAPDQLPT
DKPRYLMGVGKPDDIVGAVKRGIDMMDCVLPSRSGRTGQAWTRRGQVNIKNARHADDPRPLDEDCTCPACRNYSRAYLHH
VFRAQEMISGMLLTWHNLHYYQELMQTMRDAIAANDFAGFEARFHADRAEGDIVPL
>Mature_375_residues
TRASSFRLDATSGKARTGVIQTPRGDIRTPAFMPVGTAATVKAMMPESVAATGADILLGNTYHLMLRPTAERIANLGGLH
SFMNWDKPILTDSGGFQVMSLADLRKLTEEGVTFRSHIDGSKHLLSPERSMEIQKLLGSDIVMCFDECPALPADRKALDD
SMRLSMRWAARSKEAFGDRPGYALFGIQQGGLEEDLREESAKALREIGFDGYAVGGLAVGEGQAAMFGCLDFAPDQLPTD
KPRYLMGVGKPDDIVGAVKRGIDMMDCVLPSRSGRTGQAWTRRGQVNIKNARHADDPRPLDEDCTCPACRNYSRAYLHHV
FRAQEMISGMLLTWHNLHYYQELMQTMRDAIAANDFAGFEARFHADRAEGDIVPL

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=355, Percent_Identity=42.2535211267606, Blast_Score=292, Evalue=4e-79,
Organism=Homo sapiens, GI13375872, Length=293, Percent_Identity=24.9146757679181, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1786607, Length=374, Percent_Identity=55.0802139037433, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17544680, Length=358, Percent_Identity=41.0614525139665, Blast_Score=270, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI25146502, Length=133, Percent_Identity=27.0676691729323, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19920510, Length=366, Percent_Identity=43.4426229508197, Blast_Score=312, Evalue=2e-85,
Organism=Drosophila melanogaster, GI21355311, Length=253, Percent_Identity=24.901185770751, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_JANSC (Q28PC5)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_510462.1
- ProteinModelPortal:   Q28PC5
- SMR:   Q28PC5
- STRING:   Q28PC5
- GeneID:   3934983
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_2520
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- PhylomeDB:   Q28PC5
- ProtClustDB:   PRK00112
- BioCyc:   JSP290400:JANN_2520-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 41390; Mature: 41259

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: ACT_SITE 93-93 BINDING 94-94

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRASSFRLDATSGKARTGVIQTPRGDIRTPAFMPVGTAATVKAMMPESVAATGADILLG
CCCCCCEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHCCHHHHHCCCEEEEC
NTYHLMLRPTAERIANLGGLHSFMNWDKPILTDSGGFQVMSLADLRKLTEEGVTFRSHID
CEEEEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEHHHHHHHHHHHCCEEEHHCC
GSKHLLSPERSMEIQKLLGSDIVMCFDECPALPADRKALDDSMRLSMRWAARSKEAFGDR
CCHHHCCCHHHHHHHHHHCCHHEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHCHHHCCCC
PGYALFGIQQGGLEEDLREESAKALREIGFDGYAVGGLAVGEGQAAMFGCLDFAPDQLPT
CCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCEEECCCCCHHHHHHHCCCCCCCC
DKPRYLMGVGKPDDIVGAVKRGIDMMDCVLPSRSGRTGQAWTRRGQVNIKNARHADDPRP
CCCCEEEECCCCHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHCCCEEECCCCCCCCCCC
LDEDCTCPACRNYSRAYLHHVFRAQEMISGMLLTWHNLHYYQELMQTMRDAIAANDFAGF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EARFHADRAEGDIVPL
HHEEECCCCCCCCCCC
>Mature Secondary Structure 
TRASSFRLDATSGKARTGVIQTPRGDIRTPAFMPVGTAATVKAMMPESVAATGADILLG
CCCCCEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHCCHHHHHCCCEEEEC
NTYHLMLRPTAERIANLGGLHSFMNWDKPILTDSGGFQVMSLADLRKLTEEGVTFRSHID
CEEEEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEHHHHHHHHHHHCCEEEHHCC
GSKHLLSPERSMEIQKLLGSDIVMCFDECPALPADRKALDDSMRLSMRWAARSKEAFGDR
CCHHHCCCHHHHHHHHHHCCHHEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHCHHHCCCC
PGYALFGIQQGGLEEDLREESAKALREIGFDGYAVGGLAVGEGQAAMFGCLDFAPDQLPT
CCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCEEECCCCCHHHHHHHCCCCCCCC
DKPRYLMGVGKPDDIVGAVKRGIDMMDCVLPSRSGRTGQAWTRRGQVNIKNARHADDPRP
CCCCEEEECCCCHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHCCCEEECCCCCCCCCCC
LDEDCTCPACRNYSRAYLHHVFRAQEMISGMLLTWHNLHYYQELMQTMRDAIAANDFAGF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EARFHADRAEGDIVPL
HHEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA