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The map label for this gene is hipO [H]

Identifier: 89054834

GI number: 89054834

Start: 2349835

End: 2350995

Strand: Reverse

Name: hipO [H]

Synonym: Jann_2343

Alternate gene names: 89054834

Gene position: 2350995-2349835 (Counterclockwise)

Preceding gene: 89054841

Following gene: 89054833

Centisome position: 54.45

GC content: 64.0

Gene sequence:

>1161_bases
TTGCCCGTCATCAATTCCATCGCCGCCATGGCCCCAGAGATGAAAACCTGGCGCAGACATCTGCACGCGCATCCTGAATT
GAGCTTCGACTGCCATGGGACGGCGGCCTTCGTGGTGGACCGGCTGAAAGCGTTCGGCATCACCGACATCCATGAGGGGA
TCGCGACCAGCGGTCTTGTGGCGATCATCGACAGCGGGCGAGCGGGGCCCACAATCGGCCTGCGGGCCGATATGGATGCC
TTGCCGATCCTGGAGGCGACGGGGGCGGAGCATGCCTCTACGGTGCCGGGCAAAATGCACGCCTGCGGCCATGATGGTCA
TACGGCGATGCTTCTGGGGGCGGCGAAATATCTGGTGGAGACGCGGAATTTCACTGGTCGTGTGGCGTTGATCTTCCAGC
CAGCCGAGGAAGACGGCGGCGGCGGGGAGGTCATGGTGCAGGAGGGCGCGATGGACCGGTTCGACATCAGCCGCGTCTTC
GCCATCCACAACATCCCCGGCGCGCCGGAAGGGAGCTTCTTCACCACACCCGGCCCGATCATGGCCGCGGTCGACACCAT
TACGGTTGAGATTACCGGACAGGGCGGGCACGGGGCCTATCCGCAGGACACCATTGACCCGATCCCGCCCGCCATGGCCA
TTGCGCAAGGTTTTGGGACCATCGTGTCGCGCAACACCCGCTCCCTCGACGATCTGGTGATCTCGGTCACGCAGATCCAC
GCAGGCGACGCCAGCAACGTGATCCCGTCCCATGCCATGATCAATGGCACCGTCCGCACGTTTGATCCAGCAGTGCAGGA
CATGGTGGCGCGCCGTATGGGCGAGATCGTCGATGGCACGGCCGCGGCCTACGGCGTCACCGCCAAGCTGACCTATGAGC
GTGGCTACCCCGCGACCATCAATGACCCAGACCAGACGGCCTTTGCCGTCGGCGTCGCGCAGGAGGTGGTGGGCGAGGGC
GCGGTCATCGACAATTCCAACCGCGAGATGGGGGCGGAGGATTTCTCCTACATGCTGCAAGCCCGCCCCGGCGCGTATTT
GTTTCTGGGCGCGGGCGAGGGTGCGGGGCTGCATCACCCTGGATTTGACTTCAACGACGATATCGCACCAATCGGGGCCA
GTCTGCTGGCAAAAATCGTGGAGACGGCCAATCCCGCATAG

Upstream 100 bases:

>100_bases
TCCAAGGGATGCGCCATGGCGCGGGTAAGGGCAAGTGGTCCTTGCTCCGACCGGCCTCGCGGCCCAAAGTGGTCGGGCGC
CGCACCCTCAGGAGGTCCCA

Downstream 100 bases:

>100_bases
CCCCCCCATGATTGCCACCGCATCCCGCCAGCTTATGTCTTTGCCATGATCAGCAAATGGCTTTGGACCTTGCGCAGCAT
CCTGCGCCAGCTTTGGGTAC

Product: peptidase M20D, amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MPVINSIAAMAPEMKTWRRHLHAHPELSFDCHGTAAFVVDRLKAFGITDIHEGIATSGLVAIIDSGRAGPTIGLRADMDA
LPILEATGAEHASTVPGKMHACGHDGHTAMLLGAAKYLVETRNFTGRVALIFQPAEEDGGGGEVMVQEGAMDRFDISRVF
AIHNIPGAPEGSFFTTPGPIMAAVDTITVEITGQGGHGAYPQDTIDPIPPAMAIAQGFGTIVSRNTRSLDDLVISVTQIH
AGDASNVIPSHAMINGTVRTFDPAVQDMVARRMGEIVDGTAAAYGVTAKLTYERGYPATINDPDQTAFAVGVAQEVVGEG
AVIDNSNREMGAEDFSYMLQARPGAYLFLGAGEGAGLHHPGFDFNDDIAPIGASLLAKIVETANPA

Sequences:

>Translated_386_residues
MPVINSIAAMAPEMKTWRRHLHAHPELSFDCHGTAAFVVDRLKAFGITDIHEGIATSGLVAIIDSGRAGPTIGLRADMDA
LPILEATGAEHASTVPGKMHACGHDGHTAMLLGAAKYLVETRNFTGRVALIFQPAEEDGGGGEVMVQEGAMDRFDISRVF
AIHNIPGAPEGSFFTTPGPIMAAVDTITVEITGQGGHGAYPQDTIDPIPPAMAIAQGFGTIVSRNTRSLDDLVISVTQIH
AGDASNVIPSHAMINGTVRTFDPAVQDMVARRMGEIVDGTAAAYGVTAKLTYERGYPATINDPDQTAFAVGVAQEVVGEG
AVIDNSNREMGAEDFSYMLQARPGAYLFLGAGEGAGLHHPGFDFNDDIAPIGASLLAKIVETANPA
>Mature_385_residues
PVINSIAAMAPEMKTWRRHLHAHPELSFDCHGTAAFVVDRLKAFGITDIHEGIATSGLVAIIDSGRAGPTIGLRADMDAL
PILEATGAEHASTVPGKMHACGHDGHTAMLLGAAKYLVETRNFTGRVALIFQPAEEDGGGGEVMVQEGAMDRFDISRVFA
IHNIPGAPEGSFFTTPGPIMAAVDTITVEITGQGGHGAYPQDTIDPIPPAMAIAQGFGTIVSRNTRSLDDLVISVTQIHA
GDASNVIPSHAMINGTVRTFDPAVQDMVARRMGEIVDGTAAAYGVTAKLTYERGYPATINDPDQTAFAVGVAQEVVGEGA
VIDNSNREMGAEDFSYMLQARPGAYLFLGAGEGAGLHHPGFDFNDDIAPIGASLLAKIVETANPA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=431, Percent_Identity=27.8422273781903, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 40421; Mature: 40290

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVINSIAAMAPEMKTWRRHLHAHPELSFDCHGTAAFVVDRLKAFGITDIHEGIATSGLV
CCCHHHHHHHCCHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHCCCCHHHHHHHCCCEE
AIIDSGRAGPTIGLRADMDALPILEATGAEHASTVPGKMHACGHDGHTAMLLGAAKYLVE
EEEECCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCEEECCCCCCEEHHHHHHHHHHH
TRNFTGRVALIFQPAEEDGGGGEVMVQEGAMDRFDISRVFAIHNIPGAPEGSFFTTPGPI
CCCCCCEEEEEEEECCCCCCCCEEEEECCCCCHHHHEEEEEEECCCCCCCCCEECCCCCC
MAAVDTITVEITGQGGHGAYPQDTIDPIPPAMAIAQGFGTIVSRNTRSLDDLVISVTQIH
EEEEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHEEEEEEE
AGDASNVIPSHAMINGTVRTFDPAVQDMVARRMGEIVDGTAAAYGVTAKLTYERGYPATI
CCCCCCCCCCCEEECCCHHHCCHHHHHHHHHHHHHHHCCCHHHEEEEEEEEECCCCCCCC
NDPDQTAFAVGVAQEVVGEGAVIDNSNREMGAEDFSYMLQARPGAYLFLGAGEGAGLHHP
CCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCC
GFDFNDDIAPIGASLLAKIVETANPA
CCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PVINSIAAMAPEMKTWRRHLHAHPELSFDCHGTAAFVVDRLKAFGITDIHEGIATSGLV
CCHHHHHHHCCHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHCCCCHHHHHHHCCCEE
AIIDSGRAGPTIGLRADMDALPILEATGAEHASTVPGKMHACGHDGHTAMLLGAAKYLVE
EEEECCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCEEECCCCCCEEHHHHHHHHHHH
TRNFTGRVALIFQPAEEDGGGGEVMVQEGAMDRFDISRVFAIHNIPGAPEGSFFTTPGPI
CCCCCCEEEEEEEECCCCCCCCEEEEECCCCCHHHHEEEEEEECCCCCCCCCEECCCCCC
MAAVDTITVEITGQGGHGAYPQDTIDPIPPAMAIAQGFGTIVSRNTRSLDDLVISVTQIH
EEEEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHEEEEEEE
AGDASNVIPSHAMINGTVRTFDPAVQDMVARRMGEIVDGTAAAYGVTAKLTYERGYPATI
CCCCCCCCCCCEEECCCHHHCCHHHHHHHHHHHHHHHCCCHHHEEEEEEEEECCCCCCCC
NDPDQTAFAVGVAQEVVGEGAVIDNSNREMGAEDFSYMLQARPGAYLFLGAGEGAGLHHP
CCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCC
GFDFNDDIAPIGASLLAKIVETANPA
CCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]