Click here to switch to the map view.

The map label for this gene is prpE [C]

Identifier: 89054789

GI number: 89054789

Start: 2303186

End: 2305087

Strand: Reverse

Name: prpE [C]

Synonym: Jann_2298

Alternate gene names: 89054789

Gene position: 2305087-2303186 (Counterclockwise)

Preceding gene: 89054793

Following gene: 89054788

Centisome position: 53.38

GC content: 62.83

Gene sequence:

>1902_bases
ATGGACTACAAGACGATTTACGCCACCGCCGCGACAGACCCCGAGGCGTTCTGGCTGGAGGCCGCACGGGGCATTGACTG
GACGACCCCGCCGACGATGGCATTCACGCCCGAATCCGGCGTCTATGGGCGCTGGTTTGAGGGGGCCACCGGCAATACCT
GTTACAACGCCGTTGATCGCCATGTCGCGGCCGGGCGTGGTGCGCAGCCCGCGATCATCTACGACAGCCCGGTCACGGAC
ACCCGGCGGACGCTGACCTATGCGGATCTGCTCGATGAGGTCTCAACTCTGGGCGCGGTTTTGCAGGGCATGGGCGTGGG
GCAGGGGGATCGCGTCATCATCTATATGCCGATGGTGGCCGAGGCCGTTGTCGCCATGCTTGCCTGTGCGCGGATCGGTG
CGGTGCATTCGGTTGTGTTCGGCGGGTTCGCCGCGGCCGAGCTGGCCACACGGATTGACGACGCGGGCGCGGTTGCGGTG
ATCGCCGCGTCCTGCGGGATTGAGCCGGGGCGGGTGATTGACTACATGAGCCTTGTGTCAGAAGCGATTGATATTGCATC
GAATAAGCCATCCGGATGTTTGGTTCTCCAGCGCGATATTCAGCCCTGTGACTTGACCCCAGACCGCGACCTGGATTGGG
CCGATGAGATGACGCGCGCCCGTGTCGAGGGCCGCCGCGCGCCCTGCGCTGAGGTCGCCGCCACGGACCCCGCCTATATC
CTCTACACCTCCGGCACGACGGGCCAGCCCAAGGGCGTCGTGCGTGACACTGGCGGCCATATGGTGGCGCTGCACTGGAC
GATGGAAAACATCTACGGCGTCAAACCCGGCGAAGTCTTCTGGGCCGCCTCCGACGTCGGCTGGGTCGTGGGGCATTCCT
ACATCTGCTACGCGCCGCTTTTGGCGGGCTGCACCACAATGGTGTTTGAAGGCAAACCGATTGGCACGCCCGATGCCGGC
ACGTTCTGGCGGGTGATCGCGGAGCATGATGTCAGCGTGTTGTTCACCGCACCCACAGCCTTTCGCGCGATCCGTGGACA
GGACCCTGAGGGCGACTTCCTCAATCATTACAACATCTCCAGCCTTCGGACGCTGTTCCTGGCGGGCGAACGCTCGGACC
CCGCAACCTTGCAATGGGCGGAGGCGCAACTGGGCGTGCCGGTCATCGACCATTGGTGGCAGACCGAGACCGGATGGGCG
ATTTCCGCGATCCCGATGGGGATTGAGGCATTGCCGGTGAAGCACGGATCGCCCGGCGTTCCCATGCCCGGATATGACGT
GCAGGTCCTCGATGATGCGGGCCACCCCGTGCCCAATGGCACACTCGGCAATATTGTCGTGAAACTGCCGCTGCCGCCGT
CGTGCCTGCCAACGCTCTGGAATGCGGAAGAGCGGTTCCTCAGCTCCTACCTCAATGAATTTCCTGGATATTATGCGACG
TCGGACGCCGGTATTCTGGACGAGGACGGCTACCTGCACATCATGGCGCGGACCGACGATATCATCAATGTTGCGGGTCA
CAGGCTGTCGACCGGCGCGATGGAGGAGGTGCTGGCGCACCATGACGCAGTCGCCGAATGTGCCGTCGTGGGCAAGGCTG
ATGCGCTGAAAGGCCAGGTTCCGTTGGGGTTCTTCGTCCTGAAATCCGGCATCGCCCAGGACCCCGACGACATCGCACGC
GAGCTGGTGAAAAAGGTCCGCGACGACATTGGTGCTGTGGCCGCGTTCAAGCTGGCCGTGGGTGTGAAACGCTTGCCCAA
GACACGCTCCGGCAAGGTCTTGCGTGGCACGATGCAGAAGATTGCAGATGCGCAGGCCTACAAGATGCCAGCGGCGATTG
ATGATCCGGCCATTCTGGATGAGATTGCTGAAGCGCTTACCAACAAAGGACTGATCGGATGA

Upstream 100 bases:

>100_bases
TGTGTCGCAGATAGCGTTAACGCACATTGACCGCGCCGGGAGCGGGCCGCAACCTCAGGGCAAAGATCGTCCGGGGAGAC
GGCGACAAGGGATGGGATGC

Downstream 100 bases:

>100_bases
TTGAACGGATAGACCCGGGCATACGCTCGTCGAAGATCGTGAAACACAATGGCGTGGTCTATCTGACCGGCCAGGTCGGC
GAGGGCGCTGATATCACCGA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MDYKTIYATAATDPEAFWLEAARGIDWTTPPTMAFTPESGVYGRWFEGATGNTCYNAVDRHVAAGRGAQPAIIYDSPVTD
TRRTLTYADLLDEVSTLGAVLQGMGVGQGDRVIIYMPMVAEAVVAMLACARIGAVHSVVFGGFAAAELATRIDDAGAVAV
IAASCGIEPGRVIDYMSLVSEAIDIASNKPSGCLVLQRDIQPCDLTPDRDLDWADEMTRARVEGRRAPCAEVAATDPAYI
LYTSGTTGQPKGVVRDTGGHMVALHWTMENIYGVKPGEVFWAASDVGWVVGHSYICYAPLLAGCTTMVFEGKPIGTPDAG
TFWRVIAEHDVSVLFTAPTAFRAIRGQDPEGDFLNHYNISSLRTLFLAGERSDPATLQWAEAQLGVPVIDHWWQTETGWA
ISAIPMGIEALPVKHGSPGVPMPGYDVQVLDDAGHPVPNGTLGNIVVKLPLPPSCLPTLWNAEERFLSSYLNEFPGYYAT
SDAGILDEDGYLHIMARTDDIINVAGHRLSTGAMEEVLAHHDAVAECAVVGKADALKGQVPLGFFVLKSGIAQDPDDIAR
ELVKKVRDDIGAVAAFKLAVGVKRLPKTRSGKVLRGTMQKIADAQAYKMPAAIDDPAILDEIAEALTNKGLIG

Sequences:

>Translated_633_residues
MDYKTIYATAATDPEAFWLEAARGIDWTTPPTMAFTPESGVYGRWFEGATGNTCYNAVDRHVAAGRGAQPAIIYDSPVTD
TRRTLTYADLLDEVSTLGAVLQGMGVGQGDRVIIYMPMVAEAVVAMLACARIGAVHSVVFGGFAAAELATRIDDAGAVAV
IAASCGIEPGRVIDYMSLVSEAIDIASNKPSGCLVLQRDIQPCDLTPDRDLDWADEMTRARVEGRRAPCAEVAATDPAYI
LYTSGTTGQPKGVVRDTGGHMVALHWTMENIYGVKPGEVFWAASDVGWVVGHSYICYAPLLAGCTTMVFEGKPIGTPDAG
TFWRVIAEHDVSVLFTAPTAFRAIRGQDPEGDFLNHYNISSLRTLFLAGERSDPATLQWAEAQLGVPVIDHWWQTETGWA
ISAIPMGIEALPVKHGSPGVPMPGYDVQVLDDAGHPVPNGTLGNIVVKLPLPPSCLPTLWNAEERFLSSYLNEFPGYYAT
SDAGILDEDGYLHIMARTDDIINVAGHRLSTGAMEEVLAHHDAVAECAVVGKADALKGQVPLGFFVLKSGIAQDPDDIAR
ELVKKVRDDIGAVAAFKLAVGVKRLPKTRSGKVLRGTMQKIADAQAYKMPAAIDDPAILDEIAEALTNKGLIG
>Mature_633_residues
MDYKTIYATAATDPEAFWLEAARGIDWTTPPTMAFTPESGVYGRWFEGATGNTCYNAVDRHVAAGRGAQPAIIYDSPVTD
TRRTLTYADLLDEVSTLGAVLQGMGVGQGDRVIIYMPMVAEAVVAMLACARIGAVHSVVFGGFAAAELATRIDDAGAVAV
IAASCGIEPGRVIDYMSLVSEAIDIASNKPSGCLVLQRDIQPCDLTPDRDLDWADEMTRARVEGRRAPCAEVAATDPAYI
LYTSGTTGQPKGVVRDTGGHMVALHWTMENIYGVKPGEVFWAASDVGWVVGHSYICYAPLLAGCTTMVFEGKPIGTPDAG
TFWRVIAEHDVSVLFTAPTAFRAIRGQDPEGDFLNHYNISSLRTLFLAGERSDPATLQWAEAQLGVPVIDHWWQTETGWA
ISAIPMGIEALPVKHGSPGVPMPGYDVQVLDDAGHPVPNGTLGNIVVKLPLPPSCLPTLWNAEERFLSSYLNEFPGYYAT
SDAGILDEDGYLHIMARTDDIINVAGHRLSTGAMEEVLAHHDAVAECAVVGKADALKGQVPLGFFVLKSGIAQDPDDIAR
ELVKKVRDDIGAVAAFKLAVGVKRLPKTRSGKVLRGTMQKIADAQAYKMPAAIDDPAILDEIAEALTNKGLIG

Specific function: Catalyzes The Synthesis Of Propionyl-CoA From Propionate And CoA. Also Converts Acetate To Acetyl-CoA But With A Lower Specific Activity (By Similarity). [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=629, Percent_Identity=52.3052464228935, Blast_Score=679, Evalue=0.0,
Organism=Homo sapiens, GI8923896, Length=660, Percent_Identity=34.5454545454545, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI253970489, Length=673, Percent_Identity=33.8781575037147, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI28416953, Length=635, Percent_Identity=36.2204724409449, Blast_Score=347, Evalue=2e-95,
Organism=Homo sapiens, GI38505220, Length=525, Percent_Identity=28, Blast_Score=187, Evalue=3e-47,
Organism=Homo sapiens, GI115511026, Length=543, Percent_Identity=27.0718232044199, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI58082049, Length=519, Percent_Identity=26.3969171483622, Blast_Score=172, Evalue=8e-43,
Organism=Homo sapiens, GI157311624, Length=519, Percent_Identity=26.3969171483622, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI157311622, Length=519, Percent_Identity=26.3969171483622, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI122937307, Length=505, Percent_Identity=26.3366336633663, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI42544132, Length=516, Percent_Identity=24.8062015503876, Blast_Score=159, Evalue=8e-39,
Organism=Homo sapiens, GI31982927, Length=591, Percent_Identity=23.1810490693739, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI195947378, Length=409, Percent_Identity=23.960880195599, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI42544134, Length=376, Percent_Identity=22.0744680851064, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI187761345, Length=398, Percent_Identity=26.1306532663317, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI187761343, Length=398, Percent_Identity=26.1306532663317, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786529, Length=635, Percent_Identity=45.3543307086614, Blast_Score=512, Evalue=1e-146,
Organism=Escherichia coli, GI1790505, Length=636, Percent_Identity=37.2641509433962, Blast_Score=390, Evalue=1e-109,
Organism=Escherichia coli, GI1788107, Length=552, Percent_Identity=25.1811594202899, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI145693145, Length=525, Percent_Identity=23.4285714285714, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI221142682, Length=547, Percent_Identity=22.1206581352834, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1786801, Length=507, Percent_Identity=24.2603550295858, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1786810, Length=544, Percent_Identity=22.2426470588235, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71982997, Length=627, Percent_Identity=34.2902711323764, Blast_Score=321, Evalue=7e-88,
Organism=Caenorhabditis elegans, GI71983001, Length=627, Percent_Identity=34.2902711323764, Blast_Score=321, Evalue=7e-88,
Organism=Caenorhabditis elegans, GI71985884, Length=579, Percent_Identity=23.6614853195164, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17569507, Length=599, Percent_Identity=21.0350584307179, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994703, Length=549, Percent_Identity=22.4043715846995, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71994690, Length=549, Percent_Identity=22.4043715846995, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71994694, Length=549, Percent_Identity=22.4043715846995, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17557194, Length=566, Percent_Identity=22.6148409893993, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17531443, Length=519, Percent_Identity=22.1579961464355, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538037, Length=568, Percent_Identity=21.4788732394366, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560140, Length=546, Percent_Identity=23.4432234432234, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319264, Length=649, Percent_Identity=36.8258859784284, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323182, Length=655, Percent_Identity=36.4885496183206, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6319699, Length=557, Percent_Identity=22.6211849192101, Blast_Score=90, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21355225, Length=633, Percent_Identity=44.5497630331754, Blast_Score=575, Evalue=1e-164,
Organism=Drosophila melanogaster, GI24667955, Length=629, Percent_Identity=37.5198728139905, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI62472339, Length=543, Percent_Identity=38.6740331491713, Blast_Score=344, Evalue=9e-95,
Organism=Drosophila melanogaster, GI24667959, Length=507, Percent_Identity=37.8698224852071, Blast_Score=311, Evalue=8e-85,
Organism=Drosophila melanogaster, GI24648260, Length=364, Percent_Identity=26.9230769230769, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21355181, Length=521, Percent_Identity=22.2648752399232, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24648257, Length=363, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922652, Length=309, Percent_Identity=25.2427184466019, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21356441, Length=527, Percent_Identity=21.0626185958254, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356947, Length=365, Percent_Identity=22.7397260273973, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.17 [C]

Molecular weight: Translated: 67714; Mature: 67714

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYKTIYATAATDPEAFWLEAARGIDWTTPPTMAFTPESGVYGRWFEGATGNTCYNAVDR
CCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCCEECCCCCCHHHHHHHH
HVAAGRGAQPAIIYDSPVTDTRRTLTYADLLDEVSTLGAVLQGMGVGQGDRVIIYMPMVA
HHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHH
EAVVAMLACARIGAVHSVVFGGFAAAELATRIDDAGAVAVIAASCGIEPGRVIDYMSLVS
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHH
EAIDIASNKPSGCLVLQRDIQPCDLTPDRDLDWADEMTRARVEGRRAPCAEVAATDPAYI
HHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCEE
LYTSGTTGQPKGVVRDTGGHMVALHWTMENIYGVKPGEVFWAASDVGWVVGHSYICYAPL
EEECCCCCCCCCCEEECCCCEEEEEEEEHHHCCCCCCCEEEEECCCCHHHCCCHHHHHHH
LAGCTTMVFEGKPIGTPDAGTFWRVIAEHDVSVLFTAPTAFRAIRGQDPEGDFLNHYNIS
HHCCCEEEECCCCCCCCCCCHHHHHEECCCCEEEEECCHHHHHHCCCCCCCCCCCCCCHH
SLRTLFLAGERSDPATLQWAEAQLGVPVIDHWWQTETGWAISAIPMGIEALPVKHGSPGV
HHEEEEEECCCCCCCEEEEHHHHCCCCHHHHCCCCCCCCEEEEECCCCEEEEECCCCCCC
PMPGYDVQVLDDAGHPVPNGTLGNIVVKLPLPPSCLPTLWNAEERFLSSYLNEFPGYYAT
CCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHCCHHHHHHHHHHHHCCCCEEC
SDAGILDEDGYLHIMARTDDIINVAGHRLSTGAMEEVLAHHDAVAECAVVGKADALKGQV
CCCCCCCCCCEEEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCC
PLGFFVLKSGIAQDPDDIARELVKKVRDDIGAVAAFKLAVGVKRLPKTRSGKVLRGTMQK
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
IADAQAYKMPAAIDDPAILDEIAEALTNKGLIG
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDYKTIYATAATDPEAFWLEAARGIDWTTPPTMAFTPESGVYGRWFEGATGNTCYNAVDR
CCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCCEECCCCCCHHHHHHHH
HVAAGRGAQPAIIYDSPVTDTRRTLTYADLLDEVSTLGAVLQGMGVGQGDRVIIYMPMVA
HHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHH
EAVVAMLACARIGAVHSVVFGGFAAAELATRIDDAGAVAVIAASCGIEPGRVIDYMSLVS
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHH
EAIDIASNKPSGCLVLQRDIQPCDLTPDRDLDWADEMTRARVEGRRAPCAEVAATDPAYI
HHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCEE
LYTSGTTGQPKGVVRDTGGHMVALHWTMENIYGVKPGEVFWAASDVGWVVGHSYICYAPL
EEECCCCCCCCCCEEECCCCEEEEEEEEHHHCCCCCCCEEEEECCCCHHHCCCHHHHHHH
LAGCTTMVFEGKPIGTPDAGTFWRVIAEHDVSVLFTAPTAFRAIRGQDPEGDFLNHYNIS
HHCCCEEEECCCCCCCCCCCHHHHHEECCCCEEEEECCHHHHHHCCCCCCCCCCCCCCHH
SLRTLFLAGERSDPATLQWAEAQLGVPVIDHWWQTETGWAISAIPMGIEALPVKHGSPGV
HHEEEEEECCCCCCCEEEEHHHHCCCCHHHHCCCCCCCCEEEEECCCCEEEEECCCCCCC
PMPGYDVQVLDDAGHPVPNGTLGNIVVKLPLPPSCLPTLWNAEERFLSSYLNEFPGYYAT
CCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHCCHHHHHHHHHHHHCCCCEEC
SDAGILDEDGYLHIMARTDDIINVAGHRLSTGAMEEVLAHHDAVAECAVVGKADALKGQV
CCCCCCCCCCEEEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCC
PLGFFVLKSGIAQDPDDIARELVKKVRDDIGAVAAFKLAVGVKRLPKTRSGKVLRGTMQK
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
IADAQAYKMPAAIDDPAILDEIAEALTNKGLIG
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 1339433 [H]