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The map label for this gene is dus [H]

Identifier: 89054727

GI number: 89054727

Start: 2240715

End: 2241758

Strand: Direct

Name: dus [H]

Synonym: Jann_2236

Alternate gene names: 89054727

Gene position: 2240715-2241758 (Clockwise)

Preceding gene: 89054722

Following gene: 89054728

Centisome position: 51.89

GC content: 65.23

Gene sequence:

>1044_bases
TTGTCTTCCCCGATGTCCCGCAGTATCCACCAAGAGACCGCCCAAGGATTAAGCGTTTTGCCCGTAACCCTCGCCGACAT
CGCACTAGACCCCCCTGTCCTGCTCGCACCGATGGCGGGGATCACGGATCTGCCGTTTCGCAGGCTGGTGGCCAGCTTTG
GCGCCGGGCTTGTGGTGAGCGAGATGGTGGCGTCGCAGGAAATGGTGGAAGCGAAGGCCTCCGTCCGGGCGCGGGCCGAG
CTTGGGTTCGGTGAGAACGCCACCGCCGTGCAACTGGCAGGCCGTGAGGCCCATTGGATGGCGGAGGCCGCCCGGATCGC
CGAGGGGCAGGGCGCGCGGATCATCGATATCAACATGGGTTGCCCGGCCAAGAAGGTCGTTGGCGGGCTGTCAGGCTCTG
CGCTGATGCGCGACCTCGACCATGCCTTACGCCTGATTGACGCCGTGGTGGGCGCCGTGAATGTGCCCGTCACGCTAAAG
ACGCGGCTGGGGTGGGATGATAAGACGCTGAATGCGCCTGCTCTCGCCCAGAGGGCAGAAACCGCTGGTATCCGGATGAT
TACCATCCATGGCCGCACCCGGTGCCAGTTCTACAAGGGCACCGCAAAGTGGGGCGCGATCCGTGCCGTGAAAGACGCCG
TGTCGATCCCCGTCATCGCCAATGGCGACATCACCGATGCGCCTGCCGCCGCGCAAGCCCTGCGCTGTTCCGGGGCCGAT
GGCGTCATGGTGGGACGTGGCATTCAGGGGCAGCCCTGGCTGGTGGCGGAGATCGGCGCGGCTTTGTTCGGCACACCGAC
GCCCGTCGCGCCCCAAGGCGATGCACTGGTCGCGATGGTCGCGGCGCATTACGAAGACATGCTGGCGTTTTATGGCCGTG
ACTTGGGCGGCAAAGTCGCGCGCAAGCATTTGGGATGGTACATGGACGGCGCGGGAACCCCGCCCGCATTGCGCAAAGAG
GTATTGACCACCAAAGATCCCGGTGCCGTGTTGAAGGCCCTGCCGCTGGCCCTCGCACAAACGTCCCAAAGGGCCGCCGC
ATGA

Upstream 100 bases:

>100_bases
TCGGGACAACAACGCGATATAGTTCTGGCGCAATGCATAAGCGCTATGCTTAATTATTGTGCAGTAGACCAAATCTGCGC
AAAATATCCGCAACCGCCCT

Downstream 100 bases:

>100_bases
GCACGCTGACGAACGCGCTTTGGGTCTCCCTCCCAATCCCTGCGCTGATCCTCGACGCCGAGGATCGCATCCGCGACCTT
AATCCCGTGGCCGAAACGTT

Product: dihydrouridine synthase TIM-barrel protein nifR3

Products: NA

Alternate protein names: Nitrogen regulation protein nifR3 [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MSSPMSRSIHQETAQGLSVLPVTLADIALDPPVLLAPMAGITDLPFRRLVASFGAGLVVSEMVASQEMVEAKASVRARAE
LGFGENATAVQLAGREAHWMAEAARIAEGQGARIIDINMGCPAKKVVGGLSGSALMRDLDHALRLIDAVVGAVNVPVTLK
TRLGWDDKTLNAPALAQRAETAGIRMITIHGRTRCQFYKGTAKWGAIRAVKDAVSIPVIANGDITDAPAAAQALRCSGAD
GVMVGRGIQGQPWLVAEIGAALFGTPTPVAPQGDALVAMVAAHYEDMLAFYGRDLGGKVARKHLGWYMDGAGTPPALRKE
VLTTKDPGAVLKALPLALAQTSQRAAA

Sequences:

>Translated_347_residues
MSSPMSRSIHQETAQGLSVLPVTLADIALDPPVLLAPMAGITDLPFRRLVASFGAGLVVSEMVASQEMVEAKASVRARAE
LGFGENATAVQLAGREAHWMAEAARIAEGQGARIIDINMGCPAKKVVGGLSGSALMRDLDHALRLIDAVVGAVNVPVTLK
TRLGWDDKTLNAPALAQRAETAGIRMITIHGRTRCQFYKGTAKWGAIRAVKDAVSIPVIANGDITDAPAAAQALRCSGAD
GVMVGRGIQGQPWLVAEIGAALFGTPTPVAPQGDALVAMVAAHYEDMLAFYGRDLGGKVARKHLGWYMDGAGTPPALRKE
VLTTKDPGAVLKALPLALAQTSQRAAA
>Mature_346_residues
SSPMSRSIHQETAQGLSVLPVTLADIALDPPVLLAPMAGITDLPFRRLVASFGAGLVVSEMVASQEMVEAKASVRARAEL
GFGENATAVQLAGREAHWMAEAARIAEGQGARIIDINMGCPAKKVVGGLSGSALMRDLDHALRLIDAVVGAVNVPVTLKT
RLGWDDKTLNAPALAQRAETAGIRMITIHGRTRCQFYKGTAKWGAIRAVKDAVSIPVIANGDITDAPAAAQALRCSGADG
VMVGRGIQGQPWLVAEIGAALFGTPTPVAPQGDALVAMVAAHYEDMLAFYGRDLGGKVARKHLGWYMDGAGTPPALRKEV
LTTKDPGAVLKALPLALAQTSQRAAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=228, Percent_Identity=31.140350877193, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI239788462, Length=228, Percent_Identity=31.140350877193, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI31742496, Length=226, Percent_Identity=32.3008849557522, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI40807366, Length=228, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI8923374, Length=253, Percent_Identity=30.4347826086957, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI1789660, Length=303, Percent_Identity=45.2145214521452, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1788462, Length=230, Percent_Identity=36.0869565217391, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI145693211, Length=300, Percent_Identity=27.3333333333333, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17543114, Length=239, Percent_Identity=31.3807531380753, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=223, Percent_Identity=30.9417040358744, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17507177, Length=265, Percent_Identity=27.9245283018868, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17510279, Length=204, Percent_Identity=30.3921568627451, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6323437, Length=304, Percent_Identity=25.3289473684211, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=230, Percent_Identity=28.695652173913, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323433, Length=262, Percent_Identity=27.0992366412214, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24585320, Length=245, Percent_Identity=32.2448979591837, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24580595, Length=256, Percent_Identity=28.515625, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19920448, Length=256, Percent_Identity=28.515625, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19921524, Length=241, Percent_Identity=26.9709543568465, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45549423, Length=239, Percent_Identity=32.2175732217573, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36161; Mature: 36030

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPMSRSIHQETAQGLSVLPVTLADIALDPPVLLAPMAGITDLPFRRLVASFGAGLVVS
CCCCHHHHHHHHHHCCCEEEHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHHHCCHHHHH
EMVASQEMVEAKASVRARAELGFGENATAVQLAGREAHWMAEAARIAEGQGARIIDINMG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEEEECCC
CPAKKVVGGLSGSALMRDLDHALRLIDAVVGAVNVPVTLKTRLGWDDKTLNAPALAQRAE
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHH
TAGIRMITIHGRTRCQFYKGTAKWGAIRAVKDAVSIPVIANGDITDAPAAAQALRCSGAD
HCCEEEEEEECCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHCCCCC
GVMVGRGIQGQPWLVAEIGAALFGTPTPVAPQGDALVAMVAAHYEDMLAFYGRDLGGKVA
CEEECCCCCCCCEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHH
RKHLGWYMDGAGTPPALRKEVLTTKDPGAVLKALPLALAQTSQRAAA
HHHCCEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSPMSRSIHQETAQGLSVLPVTLADIALDPPVLLAPMAGITDLPFRRLVASFGAGLVVS
CCCHHHHHHHHHHCCCEEEHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHHHCCHHHHH
EMVASQEMVEAKASVRARAELGFGENATAVQLAGREAHWMAEAARIAEGQGARIIDINMG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEEEECCC
CPAKKVVGGLSGSALMRDLDHALRLIDAVVGAVNVPVTLKTRLGWDDKTLNAPALAQRAE
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHH
TAGIRMITIHGRTRCQFYKGTAKWGAIRAVKDAVSIPVIANGDITDAPAAAQALRCSGAD
HCCEEEEEEECCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHCCCCC
GVMVGRGIQGQPWLVAEIGAALFGTPTPVAPQGDALVAMVAAHYEDMLAFYGRDLGGKVA
CEEECCCCCCCCEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHH
RKHLGWYMDGAGTPPALRKEVLTTKDPGAVLKALPLALAQTSQRAAA
HHHCCEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8355615 [H]