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The map label for this gene is lipA [H]

Identifier: 89054717

GI number: 89054717

Start: 2231911

End: 2232858

Strand: Direct

Name: lipA [H]

Synonym: Jann_2226

Alternate gene names: 89054717

Gene position: 2231911-2232858 (Clockwise)

Preceding gene: 89054716

Following gene: 89054722

Centisome position: 51.69

GC content: 61.5

Gene sequence:

>948_bases
ATGCGCGATCTGAAGATCCCCGAACAACGACACCCTGAAAAGGCTCGTCGGCCGGACAATCCGCAACCCAAGAAGCCGTC
CTGGATTCGCGTGAAAGCGCCGGTGGGTGAGGGATACAAGGCCACCGCCAAGATTATGCGGGATCACAAGCTGACGACGG
TCTGCGAAGAAGCCGGGTGTCCCAATGTCGGCGAATGCTGGTCCCAGGGTCATGCGACAATGATGATCATGGGGGAGGTC
TGCACCCGCGCCTGCACCTTCTGCAACATCGCCACCGGCAAACCGCCCGAGGCATTGGATGTGTTCGAGCCGGGGCGCGT
CGCCGATGCGGTGGCCAAACTCGGCCTCAAGCATGTGGTGATCACGTCCGTGGATCGCGACGATGTGGAAGATGGCGGGG
CGGAGCATTTTGCCCAGACCATTCGCGCTGTGCGCAACCGCTCCCCGGAAACGACGATTGAGATCCTGACGCCCGATTTC
ATCAAATCGACGCCCGATGCCCTGGAGGTTGTCGTGGCCGCCAAGCCCGATGTCTTTAACCACAATCTGGAAACCGTGCC
GGGCCTCTACCCCGAGGTGCGTCCGGGCGCGCGCTATTTCCATTCGCTGCGATTGTTGCAGCGGGTGAAGGACCTGGACC
CGACGATTTTCACCAAATCCGGCATCATGGTGGGCCTGGGTGAGGATCGCCAGGCGGTGTTACAGGTCATGGACGATATG
CGTGCAGCCGACATCGATTTCATGACGGTGGGACAGTATCTGCAACCAACGCCGAAGCATCACGCCGTAGATCGGTTCGT
GACGCCCGAGGAGTTCGAGGGCTACGAGAAGGCGGCCTGGGGCAAGGGGTTTCTGATGGTCTCGGCCACGCCGCTGACGC
GATCCTCCTATCACGCGGGCGATGACTTCGCGCGCCTTCGGGCCAATCGGCTGGCGAAGCTCGCTTAG

Upstream 100 bases:

>100_bases
CGCATGGTTTGCGCCTAGCGAATGGCGGCTGTGTCGAAATGGGGCCATTACAATTCGGGCGGAACAGGCTACTTATTGGG
CAGACTGACCCGGAGGCCCC

Downstream 100 bases:

>100_bases
ACATCCCTTTGCCGTTGTGGCTCATGTGCCTCTGCGCGGGACCGGTTTTTGGCTCTGACCCGGGCCGTGCAGCCTATTTT
TGTGCCACACCCATTCTGAA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MRDLKIPEQRHPEKARRPDNPQPKKPSWIRVKAPVGEGYKATAKIMRDHKLTTVCEEAGCPNVGECWSQGHATMMIMGEV
CTRACTFCNIATGKPPEALDVFEPGRVADAVAKLGLKHVVITSVDRDDVEDGGAEHFAQTIRAVRNRSPETTIEILTPDF
IKSTPDALEVVVAAKPDVFNHNLETVPGLYPEVRPGARYFHSLRLLQRVKDLDPTIFTKSGIMVGLGEDRQAVLQVMDDM
RAADIDFMTVGQYLQPTPKHHAVDRFVTPEEFEGYEKAAWGKGFLMVSATPLTRSSYHAGDDFARLRANRLAKLA

Sequences:

>Translated_315_residues
MRDLKIPEQRHPEKARRPDNPQPKKPSWIRVKAPVGEGYKATAKIMRDHKLTTVCEEAGCPNVGECWSQGHATMMIMGEV
CTRACTFCNIATGKPPEALDVFEPGRVADAVAKLGLKHVVITSVDRDDVEDGGAEHFAQTIRAVRNRSPETTIEILTPDF
IKSTPDALEVVVAAKPDVFNHNLETVPGLYPEVRPGARYFHSLRLLQRVKDLDPTIFTKSGIMVGLGEDRQAVLQVMDDM
RAADIDFMTVGQYLQPTPKHHAVDRFVTPEEFEGYEKAAWGKGFLMVSATPLTRSSYHAGDDFARLRANRLAKLA
>Mature_315_residues
MRDLKIPEQRHPEKARRPDNPQPKKPSWIRVKAPVGEGYKATAKIMRDHKLTTVCEEAGCPNVGECWSQGHATMMIMGEV
CTRACTFCNIATGKPPEALDVFEPGRVADAVAKLGLKHVVITSVDRDDVEDGGAEHFAQTIRAVRNRSPETTIEILTPDF
IKSTPDALEVVVAAKPDVFNHNLETVPGLYPEVRPGARYFHSLRLLQRVKDLDPTIFTKSGIMVGLGEDRQAVLQVMDDM
RAADIDFMTVGQYLQPTPKHHAVDRFVTPEEFEGYEKAAWGKGFLMVSATPLTRSSYHAGDDFARLRANRLAKLA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=305, Percent_Identity=43.9344262295082, Blast_Score=271, Evalue=7e-73,
Organism=Homo sapiens, GI37577164, Length=245, Percent_Identity=45.7142857142857, Blast_Score=236, Evalue=3e-62,
Organism=Escherichia coli, GI1786846, Length=280, Percent_Identity=49.2857142857143, Blast_Score=285, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI32564533, Length=259, Percent_Identity=44.4015444015444, Blast_Score=238, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6324770, Length=297, Percent_Identity=41.7508417508418, Blast_Score=256, Evalue=4e-69,
Organism=Drosophila melanogaster, GI221513272, Length=284, Percent_Identity=44.7183098591549, Blast_Score=265, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 35000; Mature: 35000

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDLKIPEQRHPEKARRPDNPQPKKPSWIRVKAPVGEGYKATAKIMRDHKLTTVCEEAGC
CCCCCCCHHCCCHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCHHHHHHHHCCC
PNVGECWSQGHATMMIMGEVCTRACTFCNIATGKPPEALDVFEPGRVADAVAKLGLKHVV
CCHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEE
ITSVDRDDVEDGGAEHFAQTIRAVRNRSPETTIEILTPDFIKSTPDALEVVVAAKPDVFN
EEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHCCCHHHEEEEECCCCHHC
HNLETVPGLYPEVRPGARYFHSLRLLQRVKDLDPTIFTKSGIMVGLGEDRQAVLQVMDDM
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCEEEECCCCHHHHHHHHHHH
RAADIDFMTVGQYLQPTPKHHAVDRFVTPEEFEGYEKAAWGKGFLMVSATPLTRSSYHAG
HHCCCCHHHHHHHHCCCCCHHHHHCCCCHHHHCCHHHHCCCCCEEEEECCCCCCCCCCCC
DDFARLRANRLAKLA
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MRDLKIPEQRHPEKARRPDNPQPKKPSWIRVKAPVGEGYKATAKIMRDHKLTTVCEEAGC
CCCCCCCHHCCCHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCHHHHHHHHCCC
PNVGECWSQGHATMMIMGEVCTRACTFCNIATGKPPEALDVFEPGRVADAVAKLGLKHVV
CCHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEE
ITSVDRDDVEDGGAEHFAQTIRAVRNRSPETTIEILTPDFIKSTPDALEVVVAAKPDVFN
EEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHCCCHHHEEEEECCCCHHC
HNLETVPGLYPEVRPGARYFHSLRLLQRVKDLDPTIFTKSGIMVGLGEDRQAVLQVMDDM
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCEEEECCCCHHHHHHHHHHH
RAADIDFMTVGQYLQPTPKHHAVDRFVTPEEFEGYEKAAWGKGFLMVSATPLTRSSYHAG
HHCCCCHHHHHHHHCCCCCHHHHHCCCCHHHHCCHHHHCCCCCEEEEECCCCCCCCCCCC
DDFARLRANRLAKLA
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA