Click here to switch to the map view.

The map label for this gene is dusA [H]

Identifier: 89054629

GI number: 89054629

Start: 2145849

End: 2146838

Strand: Direct

Name: dusA [H]

Synonym: Jann_2138

Alternate gene names: 89054629

Gene position: 2145849-2146838 (Clockwise)

Preceding gene: 89054628

Following gene: 89054630

Centisome position: 49.7

GC content: 62.42

Gene sequence:

>990_bases
ATGACGTTAAATCAAAATGCTAGGCTGTCCATCGCCCCGATGATGGATTGGACCGACCGCCATTGCCGGTATTTTCATCG
GCTGATGTCGCGGGATGCGTTGCTTTATACGGAAATGGTCACCTCTGCCGCCGTGATCAATGGTGACCGAAATCGGTTGC
TGCGTTTTGACCCGGCGGAACATCCTGTCGCGTTGCAATTGGGCGGGTCGGATCCTGCGGAGTTGGCGCACGCGGTGCGG
ATTGTGCGCGACTGGGGGTACGATGAGGTGAATTTGAACGTCGGCTGCCCCTCGGATCGGGTGCAATCGGGGTGTTTCGG
GGCGGTCCTGATGCGACAGCCCGATCTTGTGGCGGAGTGTGTCCGCGCCATGCGCGATGAATGCCCGGTCGACGTGACCG
TGAAATGCCGCATTGGGGTCGATGATCAGGACCCGGAGGACGCGTTGCCGCGTTTCCTGGAGGCCATGGTGACGGCAGGG
GTGACGCGCGTGACGATCCATGCCCGCAAAGCCTGGTTGCAGGGGCTGAGCCCGAAGCAGAACCGCGACGTGCCGCCGCT
CGATTATCCCTTGGTTTTCAGGATGAAGCAGATGTTCCCGCAGCTGCATATCTCACTCAACGGGGGGATCGCGACGCTGG
AGGAGGCGGGGGCGCATCTGGAGGACGGGCTCGACGGCGTGATGATCGGACGCGCGGCCTATCACACTCCGGCAGAGGTC
CTGTTGCAGGCTGATCAACGCATTTTTGGCCATGCAACGCCAGCCCGCAGCGCAGAGGAGGTGGCGCTGTTGATGTTGCC
TTACATCGACGCGCATCTGACCGATGGCGGCCGCCTGAACCAGATCACGCGTCATATTCTGGGCCTCTTTGCAGGGCGTC
CCGGTGCGCGTGGCTGGAAGCGCATCCTGTCAGAGGGTGCACACCAGGACGGCGCTGGCCCGGAGTTGGTAGAGCGCGCC
CTGCAAGAGGTCATCACCCGCGCGGCGTGA

Upstream 100 bases:

>100_bases
CTGTTTGTGCAATGAATCGTCGAAGTTCGGGAACTTATGGCTATCATCTCGCAGTCTGGCGTAGGTTTGAACGCCGACAC
AATTTGAAGACACACAAGAA

Downstream 100 bases:

>100_bases
TCCGATTTGAGATTGCGGGGCGGCAGTGGGGCGGATATACGACCACTTACGCGGACCCTTAGCTCAGCTGGATAGAGCGC
TGCCCTCCGAAGGCAGAGGC

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MTLNQNARLSIAPMMDWTDRHCRYFHRLMSRDALLYTEMVTSAAVINGDRNRLLRFDPAEHPVALQLGGSDPAELAHAVR
IVRDWGYDEVNLNVGCPSDRVQSGCFGAVLMRQPDLVAECVRAMRDECPVDVTVKCRIGVDDQDPEDALPRFLEAMVTAG
VTRVTIHARKAWLQGLSPKQNRDVPPLDYPLVFRMKQMFPQLHISLNGGIATLEEAGAHLEDGLDGVMIGRAAYHTPAEV
LLQADQRIFGHATPARSAEEVALLMLPYIDAHLTDGGRLNQITRHILGLFAGRPGARGWKRILSEGAHQDGAGPELVERA
LQEVITRAA

Sequences:

>Translated_329_residues
MTLNQNARLSIAPMMDWTDRHCRYFHRLMSRDALLYTEMVTSAAVINGDRNRLLRFDPAEHPVALQLGGSDPAELAHAVR
IVRDWGYDEVNLNVGCPSDRVQSGCFGAVLMRQPDLVAECVRAMRDECPVDVTVKCRIGVDDQDPEDALPRFLEAMVTAG
VTRVTIHARKAWLQGLSPKQNRDVPPLDYPLVFRMKQMFPQLHISLNGGIATLEEAGAHLEDGLDGVMIGRAAYHTPAEV
LLQADQRIFGHATPARSAEEVALLMLPYIDAHLTDGGRLNQITRHILGLFAGRPGARGWKRILSEGAHQDGAGPELVERA
LQEVITRAA
>Mature_328_residues
TLNQNARLSIAPMMDWTDRHCRYFHRLMSRDALLYTEMVTSAAVINGDRNRLLRFDPAEHPVALQLGGSDPAELAHAVRI
VRDWGYDEVNLNVGCPSDRVQSGCFGAVLMRQPDLVAECVRAMRDECPVDVTVKCRIGVDDQDPEDALPRFLEAMVTAGV
TRVTIHARKAWLQGLSPKQNRDVPPLDYPLVFRMKQMFPQLHISLNGGIATLEEAGAHLEDGLDGVMIGRAAYHTPAEVL
LQADQRIFGHATPARSAEEVALLMLPYIDAHLTDGGRLNQITRHILGLFAGRPGARGWKRILSEGAHQDGAGPELVERAL
QEVITRAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=233, Percent_Identity=29.6137339055794, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI239788483, Length=257, Percent_Identity=25.6809338521401, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI239788462, Length=238, Percent_Identity=26.4705882352941, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI40807366, Length=285, Percent_Identity=27.0175438596491, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI145693211, Length=323, Percent_Identity=55.1083591331269, Blast_Score=355, Evalue=3e-99,
Organism=Escherichia coli, GI1789660, Length=235, Percent_Identity=28.936170212766, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788462, Length=217, Percent_Identity=29.4930875576037, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25144369, Length=211, Percent_Identity=32.2274881516588, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=174, Percent_Identity=30.4597701149425, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=233, Percent_Identity=23.6051502145923, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19921524, Length=233, Percent_Identity=27.0386266094421, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24580595, Length=234, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI19920448, Length=234, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36373; Mature: 36242

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNQNARLSIAPMMDWTDRHCRYFHRLMSRDALLYTEMVTSAAVINGDRNRLLRFDPAE
CCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHEECCCCCEEEECCCC
HPVALQLGGSDPAELAHAVRIVRDWGYDEVNLNVGCPSDRVQSGCFGAVLMRQPDLVAEC
CCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCHHHHHHHCCCHHHHHH
VRAMRDECPVDVTVKCRIGVDDQDPEDALPRFLEAMVTAGVTRVTIHARKAWLQGLSPKQ
HHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCC
NRDVPPLDYPLVFRMKQMFPQLHISLNGGIATLEEAGAHLEDGLDGVMIGRAAYHTPAEV
CCCCCCCCCHHHHHHHHHCCEEEEEECCCCHHHHHCCCHHHCCCCEEEECCHHHCCHHHH
LLQADQRIFGHATPARSAEEVALLMLPYIDAHLTDGGRLNQITRHILGLFAGRPGARGWK
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
RILSEGAHQDGAGPELVERALQEVITRAA
HHHHCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLNQNARLSIAPMMDWTDRHCRYFHRLMSRDALLYTEMVTSAAVINGDRNRLLRFDPAE
CCCCCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHEECCCCCEEEECCCC
HPVALQLGGSDPAELAHAVRIVRDWGYDEVNLNVGCPSDRVQSGCFGAVLMRQPDLVAEC
CCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCHHHHHHHCCCHHHHHH
VRAMRDECPVDVTVKCRIGVDDQDPEDALPRFLEAMVTAGVTRVTIHARKAWLQGLSPKQ
HHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCC
NRDVPPLDYPLVFRMKQMFPQLHISLNGGIATLEEAGAHLEDGLDGVMIGRAAYHTPAEV
CCCCCCCCCHHHHHHHHHCCEEEEEECCCCHHHHHCCCHHHCCCCEEEECCHHHCCHHHH
LLQADQRIFGHATPARSAEEVALLMLPYIDAHLTDGGRLNQITRHILGLFAGRPGARGWK
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
RILSEGAHQDGAGPELVERALQEVITRAA
HHHHCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]