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The map label for this gene is queA

Identifier: 89054208

GI number: 89054208

Start: 1700998

End: 1702065

Strand: Direct

Name: queA

Synonym: Jann_1717

Alternate gene names: 89054208

Gene position: 1700998-1702065 (Clockwise)

Preceding gene: 89054207

Following gene: 89054209

Centisome position: 39.39

GC content: 62.83

Gene sequence:

>1068_bases
ATGAAACTGGATGATTTCGACTTCGATCTGCCCGACGGGTTGATTGCCACGCGCCCCGCGCGGCCCCGATCCTCGGCCAG
ATTGCTGGTGGCCGACGGGCCGGAGACCCATGATCTGACCGTGGCGGATCTGCCCTCCATGCTGCGGCGCGGGGACCGGT
TGGTCCTGAATGACACCCGCGTGATCCCCGCGCGGCTGACCGGCACGCGCACCCGAATGTCCGCGCAGGGAGAGGTGACG
GCCCGGATCGAAGTCACCCTGATGGAGCCGCAGGCCGATGGCACCTGGTCCGCGCTTGCCAAGCCGATGCGGAAGCTGAA
AGCGGGAGAGAAAATAATATTCTCCAATGCTTTGCAAGCTGATGTTCATGCGATGGATGAAGGATTGCGCCTGGCCTTCA
ATGTCGAAGGCGACGATTTTGACGCGGCGCTGAACGCCGCAGGTGCCATGCCGCTTCCGCCCTATATTGCGGGCAAGCGG
GCCCCCGATGCGCGCGACTATCACGACTATCAGACCATCTGGGCCAAACGCGCCGGCGCCGTAGCCGCGCCCACCGCCAG
CCTGCATTTCGACGAACACCTGATGGACGCCCTGACATCTCGTGGTGTTGATGTCACCTTTGTCACGCTGCATGTCGGTG
CGGGCACCTTCCTGCCTGTCACGGTCGACGACGTCACCACCCACAAGATGCATGCCGAATGGGGTGAGGTCCGCGCGACA
GCCGCCGCCGAGATCAACGCCACCAAGGCCGCCGGGGGGCGCATCATTCCCGTTGGCACCACGGCCCTGCGGCTGATCGA
ATCCGCCGCCCAAGACGGCACAATCCACCCCTTTGAGGGCACGACCGATATTTTCATCTATCCCGGCTATCGCTGGCAGA
TCACCGACGCGCTGATGACCAACTTTCACCTGCCCAAATCGACGCTGCTGATGCTGGTCTCCGCGCTGATGGGCAAGGAC
CGGATGGACGCCGTCTACGCCCATGCCATCGCCAACCAGTATCGGTTTTTTTCCTATGGTGACGCGTCTTTGCTCTTTCC
GTCGCAAAAGGGCCAGACGTCGCGGTGA

Upstream 100 bases:

>100_bases
ATACGCGGGTGACGGTGAACCCCCTGTCGATCCTGACGCCGGGCATTTTCCGGGACATCTTCCGCGCCCCACCGCCGGAT
TTCTCGACACAGGGTAACTG

Downstream 100 bases:

>100_bases
TTTCCGGGCAGGTCTCGGATCAGCACCTGATACGCCAACCCGCACGCATGTGAGGTCCCCATGATTACCGTTATCCGCAG
CGCCTGGGCGCTTCTTCTGG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKLDDFDFDLPDGLIATRPARPRSSARLLVADGPETHDLTVADLPSMLRRGDRLVLNDTRVIPARLTGTRTRMSAQGEVT
ARIEVTLMEPQADGTWSALAKPMRKLKAGEKIIFSNALQADVHAMDEGLRLAFNVEGDDFDAALNAAGAMPLPPYIAGKR
APDARDYHDYQTIWAKRAGAVAAPTASLHFDEHLMDALTSRGVDVTFVTLHVGAGTFLPVTVDDVTTHKMHAEWGEVRAT
AAAEINATKAAGGRIIPVGTTALRLIESAAQDGTIHPFEGTTDIFIYPGYRWQITDALMTNFHLPKSTLLMLVSALMGKD
RMDAVYAHAIANQYRFFSYGDASLLFPSQKGQTSR

Sequences:

>Translated_355_residues
MKLDDFDFDLPDGLIATRPARPRSSARLLVADGPETHDLTVADLPSMLRRGDRLVLNDTRVIPARLTGTRTRMSAQGEVT
ARIEVTLMEPQADGTWSALAKPMRKLKAGEKIIFSNALQADVHAMDEGLRLAFNVEGDDFDAALNAAGAMPLPPYIAGKR
APDARDYHDYQTIWAKRAGAVAAPTASLHFDEHLMDALTSRGVDVTFVTLHVGAGTFLPVTVDDVTTHKMHAEWGEVRAT
AAAEINATKAAGGRIIPVGTTALRLIESAAQDGTIHPFEGTTDIFIYPGYRWQITDALMTNFHLPKSTLLMLVSALMGKD
RMDAVYAHAIANQYRFFSYGDASLLFPSQKGQTSR
>Mature_355_residues
MKLDDFDFDLPDGLIATRPARPRSSARLLVADGPETHDLTVADLPSMLRRGDRLVLNDTRVIPARLTGTRTRMSAQGEVT
ARIEVTLMEPQADGTWSALAKPMRKLKAGEKIIFSNALQADVHAMDEGLRLAFNVEGDDFDAALNAAGAMPLPPYIAGKR
APDARDYHDYQTIWAKRAGAVAAPTASLHFDEHLMDALTSRGVDVTFVTLHVGAGTFLPVTVDDVTTHKMHAEWGEVRAT
AAAEINATKAAGGRIIPVGTTALRLIESAAQDGTIHPFEGTTDIFIYPGYRWQITDALMTNFHLPKSTLLMLVSALMGKD
RMDAVYAHAIANQYRFFSYGDASLLFPSQKGQTSR

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=353, Percent_Identity=46.4589235127479, Blast_Score=273, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_JANSC (Q28RM8)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_509659.1
- ProteinModelPortal:   Q28RM8
- SMR:   Q28RM8
- STRING:   Q28RM8
- GeneID:   3934165
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_1717
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- PhylomeDB:   Q28RM8
- ProtClustDB:   PRK00147
- BioCyc:   JSP290400:JANN_1717-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38674; Mature: 38674

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDDFDFDLPDGLIATRPARPRSSARLLVADGPETHDLTVADLPSMLRRGDRLVLNDTR
CCCCCCCCCCCCCCEECCCCCCCCCCEEEEECCCCCCCEEHHHHHHHHHCCCEEEEECCE
VIPARLTGTRTRMSAQGEVTARIEVTLMEPQADGTWSALAKPMRKLKAGEKIIFSNALQA
EEEEEECCCHHEECCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEECCCHHH
DVHAMDEGLRLAFNVEGDDFDAALNAAGAMPLPPYIAGKRAPDARDYHDYQTIWAKRAGA
HHHHHCCCCEEEEEECCCCHHHHHHCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHCCC
VAAPTASLHFDEHLMDALTSRGVDVTFVTLHVGAGTFLPVTVDDVTTHKMHAEWGEVRAT
EECCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEEECCCCHHHHHCCHHHHEEE
AAAEINATKAAGGRIIPVGTTALRLIESAAQDGTIHPFEGTTDIFIYPGYRWQITDALMT
EEEECCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEEHHHHHH
NFHLPKSTLLMLVSALMGKDRMDAVYAHAIANQYRFFSYGDASLLFPSQKGQTSR
HCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCEEEECCCCCCCCC
>Mature Secondary Structure
MKLDDFDFDLPDGLIATRPARPRSSARLLVADGPETHDLTVADLPSMLRRGDRLVLNDTR
CCCCCCCCCCCCCCEECCCCCCCCCCEEEEECCCCCCCEEHHHHHHHHHCCCEEEEECCE
VIPARLTGTRTRMSAQGEVTARIEVTLMEPQADGTWSALAKPMRKLKAGEKIIFSNALQA
EEEEEECCCHHEECCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEECCCHHH
DVHAMDEGLRLAFNVEGDDFDAALNAAGAMPLPPYIAGKRAPDARDYHDYQTIWAKRAGA
HHHHHCCCCEEEEEECCCCHHHHHHCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHCCC
VAAPTASLHFDEHLMDALTSRGVDVTFVTLHVGAGTFLPVTVDDVTTHKMHAEWGEVRAT
EECCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEEECCCCHHHHHCCHHHHEEE
AAAEINATKAAGGRIIPVGTTALRLIESAAQDGTIHPFEGTTDIFIYPGYRWQITDALMT
EEEECCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEEHHHHHH
NFHLPKSTLLMLVSALMGKDRMDAVYAHAIANQYRFFSYGDASLLFPSQKGQTSR
HCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA