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The map label for this gene is pcaB [H]

Identifier: 89054153

GI number: 89054153

Start: 1645821

End: 1647137

Strand: Reverse

Name: pcaB [H]

Synonym: Jann_1662

Alternate gene names: 89054153

Gene position: 1647137-1645821 (Counterclockwise)

Preceding gene: 89054154

Following gene: 89054152

Centisome position: 38.15

GC content: 68.49

Gene sequence:

>1317_bases
ATGAGCACCTCCGTCTTTGACAGCACCATCCACCGCGATCTCTTTAGCGATGCGGAGGTCGCGCGGCTTCTGTCGGACAG
CGCTGAGGTGCGCGCGATGATGCTGGTGCTGGGGGCCTTGGCGAAGGTTCAGGGCGCAGCGGGCGTGATCCCCGAGATTT
CGGGCGCGTTCCTCCACCGGGCCACAATGGAGATCCAGATTGACCCGATGGGGCTGGCCGGGGCGAACGGCGTCGTGGTG
CCGGGCCTCGTGGCGGCGATGCGGGACGCGTTGGAGGCCCCGGAGCACGCGCAATATCTGCACTGGGGTGCGACCTCCCA
GGATATTCAAGACACCGCCCAAAGCCTGCGTTTGCGGCAAGTTCTGGCCTTGATGGAGGGCCGTCTGCGCGATTTGTTAA
CGGTGTTAACCGATTTGGCAGAGGCCCATGCAGAGACACCCCAAGCCGCACGCACCTACGGGCAGTTCGCCGTGCCAAGC
AGCTTCGGCGCGCTGGTTGCCGGGTGGGGCTGGCCCGTGATCCGCCTGCTGGAGCGGTTGCCGGATCTGCGCGACTACGC
CCTTTGCGTGTCGCTTTCGGGGGCGGCGGGCACGGCCAGCCAGTTGGGGCCGGACCCCGCCGCTTTGCGCGCCGCATTGG
CCGAGGCACTGGGCCTGAGCGATCCAAAGCAGTCGTGGCATGCCGACCGCGCGCGGATCATAGAGATCGCGGGATGGCTG
AGCGGTGCCGCCGTCGCCATGGGCAAGATCGGTGAGGATGTCCTGCGCCTCAGCCGCTCGGACGTGGGCGAGGTTCGGAT
TGGTGGCGCAGGGGCCTCCTCAACCATGCCGCAAAAACAGAATCCTGTGGCGGCCTCCGCCCTTGTGGCGCTGGCCCGGA
TGGCCCCCGCTCAGGTCGCCGCGATGAGCGTCCCCCATGGGGAGGCCCGCGACGGCGCGGCCTGGTTCACCGAATGGCTG
ACCCTGCCGCCGCTCGTCTCGGCCAGCGCCCGCGCCAGCGCCTTGGCAATCGACCTGGCGCGCAACATCACGGCAGACGC
GGATGCCATGGCCGCGCACCTGCAAGATCCCCTGGGCCTGATCCATGCCGAGACCCTATCCTTTGCCCTTACCGCAACCC
TGCGCCGCCCCGATGCCCAGGCCGAGGTCAAACGCCTGACCAAGGAGGCAATCGCACATAACCGCCCCCTGCCCGATCTG
GTCACAGAGGCACACCCCGATGCGCCCCTGCCACCACTGACCCCGCCGCAAAGCCTCGGCCAGGCCCCAGCCGAGGCACG
CGCCTTCGCCCAAGCCGCCCGCGCGCATCTCGCCTAG

Upstream 100 bases:

>100_bases
TCGAATTGATGCGCGGTGTGGGGCATCTGCCCTGCGTGGAAGACCCGAAGACCTACGCCGCCCACCTCACCACATTCCTC
ACCGATATCGGGCATTTGCC

Downstream 100 bases:

>100_bases
CCGCGCCGAGGACGTGCGCAGCACGGAGGAGGCACCCCCGCCCGCAGGGCATCAAGCAGGCGCGAAGCGCCGCCCCCTTT
CCCCCTTGCACGCCCTAATC

Product: fumarate lyase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSTSVFDSTIHRDLFSDAEVARLLSDSAEVRAMMLVLGALAKVQGAAGVIPEISGAFLHRATMEIQIDPMGLAGANGVVV
PGLVAAMRDALEAPEHAQYLHWGATSQDIQDTAQSLRLRQVLALMEGRLRDLLTVLTDLAEAHAETPQAARTYGQFAVPS
SFGALVAGWGWPVIRLLERLPDLRDYALCVSLSGAAGTASQLGPDPAALRAALAEALGLSDPKQSWHADRARIIEIAGWL
SGAAVAMGKIGEDVLRLSRSDVGEVRIGGAGASSTMPQKQNPVAASALVALARMAPAQVAAMSVPHGEARDGAAWFTEWL
TLPPLVSASARASALAIDLARNITADADAMAAHLQDPLGLIHAETLSFALTATLRRPDAQAEVKRLTKEAIAHNRPLPDL
VTEAHPDAPLPPLTPPQSLGQAPAEARAFAQAARAHLA

Sequences:

>Translated_438_residues
MSTSVFDSTIHRDLFSDAEVARLLSDSAEVRAMMLVLGALAKVQGAAGVIPEISGAFLHRATMEIQIDPMGLAGANGVVV
PGLVAAMRDALEAPEHAQYLHWGATSQDIQDTAQSLRLRQVLALMEGRLRDLLTVLTDLAEAHAETPQAARTYGQFAVPS
SFGALVAGWGWPVIRLLERLPDLRDYALCVSLSGAAGTASQLGPDPAALRAALAEALGLSDPKQSWHADRARIIEIAGWL
SGAAVAMGKIGEDVLRLSRSDVGEVRIGGAGASSTMPQKQNPVAASALVALARMAPAQVAAMSVPHGEARDGAAWFTEWL
TLPPLVSASARASALAIDLARNITADADAMAAHLQDPLGLIHAETLSFALTATLRRPDAQAEVKRLTKEAIAHNRPLPDL
VTEAHPDAPLPPLTPPQSLGQAPAEARAFAQAARAHLA
>Mature_437_residues
STSVFDSTIHRDLFSDAEVARLLSDSAEVRAMMLVLGALAKVQGAAGVIPEISGAFLHRATMEIQIDPMGLAGANGVVVP
GLVAAMRDALEAPEHAQYLHWGATSQDIQDTAQSLRLRQVLALMEGRLRDLLTVLTDLAEAHAETPQAARTYGQFAVPSS
FGALVAGWGWPVIRLLERLPDLRDYALCVSLSGAAGTASQLGPDPAALRAALAEALGLSDPKQSWHADRARIIEIAGWLS
GAAVAMGKIGEDVLRLSRSDVGEVRIGGAGASSTMPQKQNPVAASALVALARMAPAQVAAMSVPHGEARDGAAWFTEWLT
LPPLVSASARASALAIDLARNITADADAMAAHLQDPLGLIHAETLSFALTATLRRPDAQAEVKRLTKEAIAHNRPLPDLV
TEAHPDAPLPPLTPPQSLGQAPAEARAFAQAARAHLA

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 45876; Mature: 45745

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSVFDSTIHRDLFSDAEVARLLSDSAEVRAMMLVLGALAKVQGAAGVIPEISGAFLHR
CCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
ATMEIQIDPMGLAGANGVVVPGLVAAMRDALEAPEHAQYLHWGATSQDIQDTAQSLRLRQ
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHEECCCCHHHHHHHHHHHHHHH
VLALMEGRLRDLLTVLTDLAEAHAETPQAARTYGQFAVPSSFGALVAGWGWPVIRLLERL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHC
PDLRDYALCVSLSGAAGTASQLGPDPAALRAALAEALGLSDPKQSWHADRARIIEIAGWL
CCHHHEEEEEEECCCCCCHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
SGAAVAMGKIGEDVLRLSRSDVGEVRIGGAGASSTMPQKQNPVAASALVALARMAPAQVA
HHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHH
AMSVPHGEARDGAAWFTEWLTLPPLVSASARASALAIDLARNITADADAMAAHLQDPLGL
HCCCCCCCCCCHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHH
IHAETLSFALTATLRRPDAQAEVKRLTKEAIAHNRPLPDLVTEAHPDAPLPPLTPPQSLG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCHHHC
QAPAEARAFAQAARAHLA
CCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STSVFDSTIHRDLFSDAEVARLLSDSAEVRAMMLVLGALAKVQGAAGVIPEISGAFLHR
CCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
ATMEIQIDPMGLAGANGVVVPGLVAAMRDALEAPEHAQYLHWGATSQDIQDTAQSLRLRQ
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHEECCCCHHHHHHHHHHHHHHH
VLALMEGRLRDLLTVLTDLAEAHAETPQAARTYGQFAVPSSFGALVAGWGWPVIRLLERL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHC
PDLRDYALCVSLSGAAGTASQLGPDPAALRAALAEALGLSDPKQSWHADRARIIEIAGWL
CCHHHEEEEEEECCCCCCHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
SGAAVAMGKIGEDVLRLSRSDVGEVRIGGAGASSTMPQKQNPVAASALVALARMAPAQVA
HHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHH
AMSVPHGEARDGAAWFTEWLTLPPLVSASARASALAIDLARNITADADAMAAHLQDPLGL
HCCCCCCCCCCHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHH
IHAETLSFALTATLRRPDAQAEVKRLTKEAIAHNRPLPDLVTEAHPDAPLPPLTPPQSLG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCHHHC
QAPAEARAFAQAARAHLA
CCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1390752 [H]