Click here to switch to the map view.

The map label for this gene is lipB

Identifier: 89053743

GI number: 89053743

Start: 1207524

End: 1208174

Strand: Reverse

Name: lipB

Synonym: Jann_1252

Alternate gene names: 89053743

Gene position: 1208174-1207524 (Counterclockwise)

Preceding gene: 89053744

Following gene: 89053742

Centisome position: 27.98

GC content: 66.36

Gene sequence:

>651_bases
ATGGTCGAATGGATCACCTCGGACGCGCCCGTCCCCTACCCCCTCGCCGTGGCCGAGATGGAGGCGCGCGCCAACGCCAT
CGCGAAAGGTGAGGCCGGAGAGGCGGTGTGGCTCCTGGAACATCCCCCGCTTTATACGGCGGGCACGTCTGCCAGAGAGA
GCGATCTGCGGGACCCGGACCGCTTCCCCGTTTTCGAGACGCGACGCGGCGGGCAGTACACGTATCACGGCCCGGGCCAG
CGAGTGGCTTACGTCATGCTGGACCTGAACACACGCGGCAAGGACGTGCGCGCCTTCGTGCAAAGGCTGGAGGCCTGGGT
CATCGCGGCTCTGGATGATTTCAACGTCACCGGCGCGGTGCGGGAGGGGCGCGTGGGCGTCTGGGTGGATCGCCCCGAAA
AGGCGCCGCTCCCCGATGGGACGCCGCGCGAGGACAAGATTGCCGCCATCGGCGTGCGCCTGCGCAAATGGGTCAGCTTT
CACGGCCTGTCGATCAATGTGGAGCCGGACCTGAGCCATTTTGATGGGATCGTGCCCTGCGGCATCGAGGGACACGGGGT
GACCAGCCTTGTGGATCTGGGCCTTCCGGTGACGATGGCGGATCTGGACGTCGCCCTGAAGCGGCAGTTTTTTGAGGTGT
TCGGGGCCTGA

Upstream 100 bases:

>100_bases
GGTGCTGACCGTGACCGGTGGGGGTGACATTCCCGTCAGCCGCGCCAATGTGGGGGCCATCAAGGATGCGGGCCTGCTGC
CCCAATAGCGGAATTGCGAC

Downstream 100 bases:

>100_bases
CGGGCGGCGCAAACGCGCGGGTCGGCCCTGGCGGGCCTTGCAGGCGCGGCCGCTCCTCCCTGCGGTCCTCGCCGCGGTGG
CGCCTGCGGCGCGGAGGAAC

Product: lipoyltransferase

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MVEWITSDAPVPYPLAVAEMEARANAIAKGEAGEAVWLLEHPPLYTAGTSARESDLRDPDRFPVFETRRGGQYTYHGPGQ
RVAYVMLDLNTRGKDVRAFVQRLEAWVIAALDDFNVTGAVREGRVGVWVDRPEKAPLPDGTPREDKIAAIGVRLRKWVSF
HGLSINVEPDLSHFDGIVPCGIEGHGVTSLVDLGLPVTMADLDVALKRQFFEVFGA

Sequences:

>Translated_216_residues
MVEWITSDAPVPYPLAVAEMEARANAIAKGEAGEAVWLLEHPPLYTAGTSARESDLRDPDRFPVFETRRGGQYTYHGPGQ
RVAYVMLDLNTRGKDVRAFVQRLEAWVIAALDDFNVTGAVREGRVGVWVDRPEKAPLPDGTPREDKIAAIGVRLRKWVSF
HGLSINVEPDLSHFDGIVPCGIEGHGVTSLVDLGLPVTMADLDVALKRQFFEVFGA
>Mature_216_residues
MVEWITSDAPVPYPLAVAEMEARANAIAKGEAGEAVWLLEHPPLYTAGTSARESDLRDPDRFPVFETRRGGQYTYHGPGQ
RVAYVMLDLNTRGKDVRAFVQRLEAWVIAALDDFNVTGAVREGRVGVWVDRPEKAPLPDGTPREDKIAAIGVRLRKWVSF
HGLSINVEPDLSHFDGIVPCGIEGHGVTSLVDLGLPVTMADLDVALKRQFFEVFGA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=192, Percent_Identity=34.8958333333333, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI87081767, Length=151, Percent_Identity=39.7350993377483, Blast_Score=115, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323268, Length=184, Percent_Identity=27.1739130434783, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355483, Length=210, Percent_Identity=35.7142857142857, Blast_Score=109, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_JANSC (Q28SZ3)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_509194.1
- ProteinModelPortal:   Q28SZ3
- SMR:   Q28SZ3
- STRING:   Q28SZ3
- GeneID:   3933697
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_1252
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   HPPVFTL
- PhylomeDB:   Q28SZ3
- ProtClustDB:   CLSK2776165
- BioCyc:   JSP290400:JANN_1252-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 23749; Mature: 23749

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 180-180

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEWITSDAPVPYPLAVAEMEARANAIAKGEAGEAVWLLEHPPLYTAGTSARESDLRDPD
CCEEECCCCCCCCCHHHHHHHHHHHHEECCCCCCEEEEEECCCCEECCCCCCCCCCCCCC
RFPVFETRRGGQYTYHGPGQRVAYVMLDLNTRGKDVRAFVQRLEAWVIAALDDFNVTGAV
CCCEEECCCCCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHEEEEECCCCCCCEE
REGRVGVWVDRPEKAPLPDGTPREDKIAAIGVRLRKWVSFHGLSINVEPDLSHFDGIVPC
ECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHCCEEEC
GIEGHGVTSLVDLGLPVTMADLDVALKRQFFEVFGA
CCCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVEWITSDAPVPYPLAVAEMEARANAIAKGEAGEAVWLLEHPPLYTAGTSARESDLRDPD
CCEEECCCCCCCCCHHHHHHHHHHHHEECCCCCCEEEEEECCCCEECCCCCCCCCCCCCC
RFPVFETRRGGQYTYHGPGQRVAYVMLDLNTRGKDVRAFVQRLEAWVIAALDDFNVTGAV
CCCEEECCCCCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHEEEEECCCCCCCEE
REGRVGVWVDRPEKAPLPDGTPREDKIAAIGVRLRKWVSFHGLSINVEPDLSHFDGIVPC
ECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHCCEEEC
GIEGHGVTSLVDLGLPVTMADLDVALKRQFFEVFGA
CCCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA