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The map label for this gene is amiD [H]

Identifier: 89053710

GI number: 89053710

Start: 1178607

End: 1179290

Strand: Reverse

Name: amiD [H]

Synonym: Jann_1219

Alternate gene names: 89053710

Gene position: 1179290-1178607 (Counterclockwise)

Preceding gene: 89053711

Following gene: 89053708

Centisome position: 27.31

GC content: 64.33

Gene sequence:

>684_bases
TTGACAGCTAAATCTTTGCAAAGCCCCAATTTCGGGCCAAGGCGCGATGGGTTGAAGCCAGAGTTGGTTGTGATCCACTA
CACCGCGATGCCGAACTGCGCATCAGCCGCAAAAGTGCTGTGCGACCCGGCGCGGGAGGTCTCCGCCCATTATTTGATCG
GGCGCGATGGCGACGTGTTGAACCTTGTGCCAGAGGACATGCGCGCTTGGCACGCAGGCAAGGGCGAATGGCGCGGGGCG
GGGGATGTGAACTCCCGCTCGATCGGGATCGAGTTGGACAATGACGGGCTGACGCCCTTCTCCGAGCCGCTGATGGCGGC
GCTCGAAACGCTCCTCCCTACGGTCCTCGCCGCATGGCGGATTGGGCCAGACGGCGTGATCGGGCATTCAGACATGGCCC
CGGGGCGCAAAATTGACCCCGGCAGGCGGTTTGACTGGCAACGACTGGCGCGGCAGGGATTGGCAGTGTGGTCGCAGGCC
TCCGGCGATACGGTTGATGCAGCGCGGTTTCGGGGCGATGCACAGGCTTTTGGGTATGGAGACATGGACGATACGCTGCT
GCTGGACGTGGTGCGGATGCGGTTCCGGCCCTGGGCGTCCGGTCCGCTGGATGGAACCGACTGCGCGATCATGGCGGACA
TTGCGGCACGCTATGGGCTTGACCGGCGCGGGCCGGGCGCATAG

Upstream 100 bases:

>100_bases
ATGGACCCTGACGGCGACGGATTCGTCTGCGGTTTTGACCCGACCGCCCTGCGCGCGTCGGCCAATGCCCCGGTCGAGAC
GTTTGAAACGCTCCCCGCGA

Downstream 100 bases:

>100_bases
CGGAAGGGTCGCGTGGAGGGCTGGATGGCCGCGAGGCTCTGTTTTTCGGAATGGGGTGTCGAGGAAAGTCCGGACTCCCT
GAAGACAAGGTGCCGGGTAA

Product: negative regulator of AmpC, AmpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MTAKSLQSPNFGPRRDGLKPELVVIHYTAMPNCASAAKVLCDPAREVSAHYLIGRDGDVLNLVPEDMRAWHAGKGEWRGA
GDVNSRSIGIELDNDGLTPFSEPLMAALETLLPTVLAAWRIGPDGVIGHSDMAPGRKIDPGRRFDWQRLARQGLAVWSQA
SGDTVDAARFRGDAQAFGYGDMDDTLLLDVVRMRFRPWASGPLDGTDCAIMADIAARYGLDRRGPGA

Sequences:

>Translated_227_residues
MTAKSLQSPNFGPRRDGLKPELVVIHYTAMPNCASAAKVLCDPAREVSAHYLIGRDGDVLNLVPEDMRAWHAGKGEWRGA
GDVNSRSIGIELDNDGLTPFSEPLMAALETLLPTVLAAWRIGPDGVIGHSDMAPGRKIDPGRRFDWQRLARQGLAVWSQA
SGDTVDAARFRGDAQAFGYGDMDDTLLLDVVRMRFRPWASGPLDGTDCAIMADIAARYGLDRRGPGA
>Mature_226_residues
TAKSLQSPNFGPRRDGLKPELVVIHYTAMPNCASAAKVLCDPAREVSAHYLIGRDGDVLNLVPEDMRAWHAGKGEWRGAG
DVNSRSIGIELDNDGLTPFSEPLMAALETLLPTVLAAWRIGPDGVIGHSDMAPGRKIDPGRRFDWQRLARQGLAVWSQAS
GDTVDAARFRGDAQAFGYGDMDDTLLLDVVRMRFRPWASGPLDGTDCAIMADIAARYGLDRRGPGA

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=235, Percent_Identity=34.468085106383, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786300, Length=164, Percent_Identity=32.3170731707317, Blast_Score=87, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 24585; Mature: 24454

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKSLQSPNFGPRRDGLKPELVVIHYTAMPNCASAAKVLCDPAREVSAHYLIGRDGDVL
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCHHHCCEEEEECCCCCEE
NLVPEDMRAWHAGKGEWRGAGDVNSRSIGIELDNDGLTPFSEPLMAALETLLPTVLAAWR
ECCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHC
IGPDGVIGHSDMAPGRKIDPGRRFDWQRLARQGLAVWSQASGDTVDAARFRGDAQAFGYG
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCC
DMDDTLLLDVVRMRFRPWASGPLDGTDCAIMADIAARYGLDRRGPGA
CCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TAKSLQSPNFGPRRDGLKPELVVIHYTAMPNCASAAKVLCDPAREVSAHYLIGRDGDVL
CCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCHHHCCEEEEECCCCCEE
NLVPEDMRAWHAGKGEWRGAGDVNSRSIGIELDNDGLTPFSEPLMAALETLLPTVLAAWR
ECCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHC
IGPDGVIGHSDMAPGRKIDPGRRFDWQRLARQGLAVWSQASGDTVDAARFRGDAQAFGYG
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCC
DMDDTLLLDVVRMRFRPWASGPLDGTDCAIMADIAARYGLDRRGPGA
CCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]