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The map label for this gene is argT [H]

Identifier: 89053623

GI number: 89053623

Start: 1094512

End: 1095234

Strand: Reverse

Name: argT [H]

Synonym: Jann_1132

Alternate gene names: 89053623

Gene position: 1095234-1094512 (Counterclockwise)

Preceding gene: 89053625

Following gene: 89053622

Centisome position: 25.36

GC content: 63.76

Gene sequence:

>723_bases
ATGAAAAACCTGATTCTGGCGACAACCGCCCTGACGCTGACCGCCGGCATGGCCTTCGCCCAGGACACCGTCCGCATGGG
CACCGAGGGCGCCTACCCCCCCTACAACTTCATCAACGATGCAGGCGAAGTGGACGGGCTGGAGCGCGCCCTGGGCGATG
AGCTGTGCGCCCGCGCCGAGCTGACCTGCGTGTGGGTCACCAACGATTGGGACAGCATCATCCCGAACCTGACGTCGCGC
AACTACGACACGATCATTGCCGGCATGAGCATCACCGAAGACCGCGAGGAAGTCATCGACTTCACCCAGGCCTACATCCC
GCCCACGCCCTCGCTTTACATGGCGATGGACGAGGATGTCTCGCTCGACGGCGCGGTGATCGCGGCCCAGACCAACACCA
TCCAGGCGGGCTATGTGGCCGGTCTTGAGGGCGTCACGCTGGTGGAATTCGCCACCCCCGATGAGACGGTCGCCGCCGTG
CGCAACGGTGAGGCCGACGCGGTTCTGGCCGACAACGACTTCCTCACCCAGATCGCCGATGAAGATCCCGACATGATGAT
CGTGCTCGATCCCGTGTCGCTTGGTGACGGCGTTGGCATGGGCCTGCGTGAGGGCGATGAGCTGATCGCCGTCTTCGACG
CGGCCATCACGTCGATGAAGGAAGACGGCACGCTGAACGCGCTGCTGGAAGAATGGTTCGGCGAAAACGCCGTGACCTGG
TAA

Upstream 100 bases:

>100_bases
TCCCATCTTGTCCTCCCGGCACAAACCTTTATCACCTTTCGCCATATTCGTCGTCGCGCCATCCGTTTGCGCGGCTGCGA
CCAACATGGAGATGAGCAAA

Downstream 100 bases:

>100_bases
CCTGATCGCGGCTCCGGGCTGACTTCGGTTGGCCCGGAGCCCATTCCATTGGCGGCGCGCCCCGATGCAGCGGGAAGCCC
GCGCCATTGCACGCCGAAAC

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKNLILATTALTLTAGMAFAQDTVRMGTEGAYPPYNFINDAGEVDGLERALGDELCARAELTCVWVTNDWDSIIPNLTSR
NYDTIIAGMSITEDREEVIDFTQAYIPPTPSLYMAMDEDVSLDGAVIAAQTNTIQAGYVAGLEGVTLVEFATPDETVAAV
RNGEADAVLADNDFLTQIADEDPDMMIVLDPVSLGDGVGMGLREGDELIAVFDAAITSMKEDGTLNALLEEWFGENAVTW

Sequences:

>Translated_240_residues
MKNLILATTALTLTAGMAFAQDTVRMGTEGAYPPYNFINDAGEVDGLERALGDELCARAELTCVWVTNDWDSIIPNLTSR
NYDTIIAGMSITEDREEVIDFTQAYIPPTPSLYMAMDEDVSLDGAVIAAQTNTIQAGYVAGLEGVTLVEFATPDETVAAV
RNGEADAVLADNDFLTQIADEDPDMMIVLDPVSLGDGVGMGLREGDELIAVFDAAITSMKEDGTLNALLEEWFGENAVTW
>Mature_240_residues
MKNLILATTALTLTAGMAFAQDTVRMGTEGAYPPYNFINDAGEVDGLERALGDELCARAELTCVWVTNDWDSIIPNLTSR
NYDTIIAGMSITEDREEVIDFTQAYIPPTPSLYMAMDEDVSLDGAVIAAQTNTIQAGYVAGLEGVTLVEFATPDETVAAV
RNGEADAVLADNDFLTQIADEDPDMMIVLDPVSLGDGVGMGLREGDELIAVFDAAITSMKEDGTLNALLEEWFGENAVTW

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=256, Percent_Identity=32.03125, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787088, Length=248, Percent_Identity=27.8225806451613, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787085, Length=244, Percent_Identity=27.0491803278689, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788648, Length=226, Percent_Identity=30.9734513274336, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788228, Length=254, Percent_Identity=25.9842519685039, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787031, Length=250, Percent_Identity=26.8, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 25776; Mature: 25776

Theoretical pI: Translated: 3.43; Mature: 3.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLILATTALTLTAGMAFAQDTVRMGTEGAYPPYNFINDAGEVDGLERALGDELCARAE
CCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCE
LTCVWVTNDWDSIIPNLTSRNYDTIIAGMSITEDREEVIDFTQAYIPPTPSLYMAMDEDV
EEEEEEECCHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCC
SLDGAVIAAQTNTIQAGYVAGLEGVTLVEFATPDETVAAVRNGEADAVLADNDFLTQIAD
CCCCEEEEEECCEECCCEEECCCCEEEEEECCCCHHHHHHHCCCCCEEEECCHHHHHHCC
EDPDMMIVLDPVSLGDGVGMGLREGDELIAVFDAAITSMKEDGTLNALLEEWFGENAVTW
CCCCEEEEECCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKNLILATTALTLTAGMAFAQDTVRMGTEGAYPPYNFINDAGEVDGLERALGDELCARAE
CCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCE
LTCVWVTNDWDSIIPNLTSRNYDTIIAGMSITEDREEVIDFTQAYIPPTPSLYMAMDEDV
EEEEEEECCHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCC
SLDGAVIAAQTNTIQAGYVAGLEGVTLVEFATPDETVAAVRNGEADAVLADNDFLTQIAD
CCCCEEEEEECCEECCCEEECCCCEEEEEECCCCHHHHHHHCCCCCEEEECCHHHHHHCC
EDPDMMIVLDPVSLGDGVGMGLREGDELIAVFDAAITSMKEDGTLNALLEEWFGENAVTW
CCCCEEEEECCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]