Click here to switch to the map view.

The map label for this gene is metF [H]

Identifier: 89053517

GI number: 89053517

Start: 978797

End: 979663

Strand: Reverse

Name: metF [H]

Synonym: Jann_1026

Alternate gene names: 89053517

Gene position: 979663-978797 (Counterclockwise)

Preceding gene: 89053520

Following gene: 89053516

Centisome position: 22.69

GC content: 64.82

Gene sequence:

>867_bases
ATGCCCACGCCCAATGTCTCTTTCGAGTTCTTTCCTCCGAAATCGCTCGAAGGCTCCTTCAAGCTCTGGGAAACCCTGGG
TGTTCTGGCGCCGCTGGACCCGACATTCGTCTCCGTCACTTATGGCGCGGGCGGCACTACCCGCAAGCTGACCCATGAGG
CCGTGACCGCGATCAACGCCAATTTCGGCGTCCCCGTGGCCGCCCATCTGACTTGCGTCGATGCCACCCGGGCCGAGACG
CTGGAGATCGCCGATCAATATGCGCAGGCGGGCATTACCCAGATCGTGGCCCTGCGCGGCGACCCACCCAAGGGCACGGC
AACGTTTCAGGCCCATCCCGATGGCTTCGGCTCGTCGGAGGACCTGGTCGCGGCGCTCGCCAAAACCGGAAAGTTCGACA
TCCGCGTGGGCGCCTACCCCGATCCGCACCCGGAGGCCGCGTCCATGCAGGCCTGCGTGGATTACCTCAAGGCCAAAATC
GACGCGGGCGCCACCTCCGCCATCACGCAGTTTTTCTTCGAGGCCGACACCTTCTTCCGCTTCCGCGACGCCTGTGCCGC
CGCTGGCATCACCGCGCCGATCCTGCCCGGTATCCTGCCGATTGAGGATTGGTCCCGCGCCCGTCGCTTTGCCACTGCCT
GCGGTGCGGTGATCCCTACATGGCTCGACGACGCCTACACCAGCGCCATCCGCGATGGCCGCGAAGATCTGCTCTCCACG
GCGATCTGCACCGAGCTGTGCACCGATCTGATCGAAGGGGGCGTGGACGACCTGCATTTCTACACGCTCAACCGCCCCGA
ATTGACACGGGATGTCTGTGCGGCCCTTGGTGTCGCCCCGAACGTTGCCTTGGCCGAGGTTGCCTGA

Upstream 100 bases:

>100_bases
TATGCATGCGAACCTCATATTAATCATGAGAGTTATGAAATTGTTGATTGCCGGAGTCTATGCGACATCTGTAAGCGACA
CATCAAGGATCGATTCCGCC

Downstream 100 bases:

>100_bases
GGGGGCTAGCCCCCTTGGCACATCCAGGGATGCGCCCACCCCCAGAGTTGTAAACCCCAGGTGAAACGGAGGGTTGCGCT
TTGCGGCCAGCCGTGGCGCA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MPTPNVSFEFFPPKSLEGSFKLWETLGVLAPLDPTFVSVTYGAGGTTRKLTHEAVTAINANFGVPVAAHLTCVDATRAET
LEIADQYAQAGITQIVALRGDPPKGTATFQAHPDGFGSSEDLVAALAKTGKFDIRVGAYPDPHPEAASMQACVDYLKAKI
DAGATSAITQFFFEADTFFRFRDACAAAGITAPILPGILPIEDWSRARRFATACGAVIPTWLDDAYTSAIRDGREDLLST
AICTELCTDLIEGGVDDLHFYTLNRPELTRDVCAALGVAPNVALAEVA

Sequences:

>Translated_288_residues
MPTPNVSFEFFPPKSLEGSFKLWETLGVLAPLDPTFVSVTYGAGGTTRKLTHEAVTAINANFGVPVAAHLTCVDATRAET
LEIADQYAQAGITQIVALRGDPPKGTATFQAHPDGFGSSEDLVAALAKTGKFDIRVGAYPDPHPEAASMQACVDYLKAKI
DAGATSAITQFFFEADTFFRFRDACAAAGITAPILPGILPIEDWSRARRFATACGAVIPTWLDDAYTSAIRDGREDLLST
AICTELCTDLIEGGVDDLHFYTLNRPELTRDVCAALGVAPNVALAEVA
>Mature_287_residues
PTPNVSFEFFPPKSLEGSFKLWETLGVLAPLDPTFVSVTYGAGGTTRKLTHEAVTAINANFGVPVAAHLTCVDATRAETL
EIADQYAQAGITQIVALRGDPPKGTATFQAHPDGFGSSEDLVAALAKTGKFDIRVGAYPDPHPEAASMQACVDYLKAKID
AGATSAITQFFFEADTFFRFRDACAAAGITAPILPGILPIEDWSRARRFATACGAVIPTWLDDAYTSAIRDGREDLLSTA
ICTELCTDLIEGGVDDLHFYTLNRPELTRDVCAALGVAPNVALAEVA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=285, Percent_Identity=36.8421052631579, Blast_Score=160, Evalue=1e-39,
Organism=Escherichia coli, GI1790377, Length=279, Percent_Identity=44.8028673835125, Blast_Score=241, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI25147441, Length=280, Percent_Identity=35, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI25147436, Length=280, Percent_Identity=35, Blast_Score=150, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6325234, Length=281, Percent_Identity=33.4519572953737, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6321313, Length=286, Percent_Identity=29.020979020979, Blast_Score=122, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24662658, Length=276, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 30604; Mature: 30473

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTPNVSFEFFPPKSLEGSFKLWETLGVLAPLDPTFVSVTYGAGGTTRKLTHEAVTAINA
CCCCCCCEEECCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCC
NFGVPVAAHLTCVDATRAETLEIADQYAQAGITQIVALRGDPPKGTATFQAHPDGFGSSE
CCCCCHHHEEEEECCHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCEEEEECCCCCCCHH
DLVAALAKTGKFDIRVGAYPDPHPEAASMQACVDYLKAKIDAGATSAITQFFFEADTFFR
HHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
FRDACAAAGITAPILPGILPIEDWSRARRFATACGAVIPTWLDDAYTSAIRDGREDLLST
HHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AICTELCTDLIEGGVDDLHFYTLNRPELTRDVCAALGVAPNVALAEVA
HHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCHHCCC
>Mature Secondary Structure 
PTPNVSFEFFPPKSLEGSFKLWETLGVLAPLDPTFVSVTYGAGGTTRKLTHEAVTAINA
CCCCCCEEECCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCC
NFGVPVAAHLTCVDATRAETLEIADQYAQAGITQIVALRGDPPKGTATFQAHPDGFGSSE
CCCCCHHHEEEEECCHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCEEEEECCCCCCCHH
DLVAALAKTGKFDIRVGAYPDPHPEAASMQACVDYLKAKIDAGATSAITQFFFEADTFFR
HHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
FRDACAAAGITAPILPGILPIEDWSRARRFATACGAVIPTWLDDAYTSAIRDGREDLLST
HHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AICTELCTDLIEGGVDDLHFYTLNRPELTRDVCAALGVAPNVALAEVA
HHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA