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The map label for this gene is ilvE [H]

Identifier: 89053359

GI number: 89053359

Start: 823672

End: 824535

Strand: Reverse

Name: ilvE [H]

Synonym: Jann_0868

Alternate gene names: 89053359

Gene position: 824535-823672 (Counterclockwise)

Preceding gene: 89053368

Following gene: 89053358

Centisome position: 19.1

GC content: 64.0

Gene sequence:

>864_bases
ATGGCCACCGGCACCAATATCAAGACCTATTTCGATGGCGCGTGGCATGACGCGGATATTCCCGTCATGCGGGCGGGCGA
CCATGGCGCCTGGCAGGGCACCACCTGCTTTGACGGGGCGCGCTACGTGGATGGCATGGCCCCGGACCTTCTGGCCCATT
GCGACCGGGTCGTGCGGTCAGCCAAGGCCCTGTCGCTCAATCCCGGCAAAACGGGGCAACAGATTTTTGACATCGTGTGG
GAGGGGCTTGGCCGCTACCCCAAAGACGTGGCCGTCTACGTGCGGCCCAACTTTTTCGGCATCGACGGCGGCCAATACGG
CATTATCCCCGCGACGACGGAGGCGGGCTTCTTCATCTGCCTGGAGGAAGTTCCCATGGCCGCACCTGACGCGGCCTCCC
GCCTCGCGACCACCCGTTTTCACCGCCCCGTGCTGAACACCGCCGTGCTGGATGCCAAGGCAGGCTGCCTCTATCCCAAC
AACGCGCGGATGCTGATGGAGGCGCGCGACAAAGGCTACGACAACGCGCTGGTCACGGACGCCATGGGCAACGTGGCCGA
AACCGCCACCGCCAACATCTTCATGGTCCGGGATGGAGAGGCGTTCACGCCAATCCCCAACGGAACCTTCCTCAACGGGA
TCACGCGACAGCGCCACATTGCCAACCTGCGCGCCGATGGTGTCACCGTGACCGAGGCCGTGCTGACCTTCCAAGATTTC
CGAGAGGCTGATGAGGTCTTCATGACCGGCAACCTCAACAAGGTCACGCCGGTGCTGGAATTCGACGGCACCCATTACCA
GCACGGTCCCGTAACCAAGCGGGCGCGGGACTTGTACTGGGACTGGGCGGCCTCCGCCGCCTGA

Upstream 100 bases:

>100_bases
GGCCAAAGGCGTGAGCATGGACTACCAGATGGGCTTCACCTCTGGACCTTCCGTCTAGGCTGCGCGTACCCTTCCCCCAC
ATCTGACGAGGGGGAAGACC

Downstream 100 bases:

>100_bases
TCCTGCCCGGGTCGCGCGCGCCCAGTGCAGGCGTTGGTAGGGCCAGGGTTGATCACACCGCGATAAGGTTAACGAATTCG
GATTTTTGTTTAGAGTGAAT

Product: aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MATGTNIKTYFDGAWHDADIPVMRAGDHGAWQGTTCFDGARYVDGMAPDLLAHCDRVVRSAKALSLNPGKTGQQIFDIVW
EGLGRYPKDVAVYVRPNFFGIDGGQYGIIPATTEAGFFICLEEVPMAAPDAASRLATTRFHRPVLNTAVLDAKAGCLYPN
NARMLMEARDKGYDNALVTDAMGNVAETATANIFMVRDGEAFTPIPNGTFLNGITRQRHIANLRADGVTVTEAVLTFQDF
READEVFMTGNLNKVTPVLEFDGTHYQHGPVTKRARDLYWDWAASAA

Sequences:

>Translated_287_residues
MATGTNIKTYFDGAWHDADIPVMRAGDHGAWQGTTCFDGARYVDGMAPDLLAHCDRVVRSAKALSLNPGKTGQQIFDIVW
EGLGRYPKDVAVYVRPNFFGIDGGQYGIIPATTEAGFFICLEEVPMAAPDAASRLATTRFHRPVLNTAVLDAKAGCLYPN
NARMLMEARDKGYDNALVTDAMGNVAETATANIFMVRDGEAFTPIPNGTFLNGITRQRHIANLRADGVTVTEAVLTFQDF
READEVFMTGNLNKVTPVLEFDGTHYQHGPVTKRARDLYWDWAASAA
>Mature_286_residues
ATGTNIKTYFDGAWHDADIPVMRAGDHGAWQGTTCFDGARYVDGMAPDLLAHCDRVVRSAKALSLNPGKTGQQIFDIVWE
GLGRYPKDVAVYVRPNFFGIDGGQYGIIPATTEAGFFICLEEVPMAAPDAASRLATTRFHRPVLNTAVLDAKAGCLYPNN
ARMLMEARDKGYDNALVTDAMGNVAETATANIFMVRDGEAFTPIPNGTFLNGITRQRHIANLRADGVTVTEAVLTFQDFR
EADEVFMTGNLNKVTPVLEFDGTHYQHGPVTKRARDLYWDWAASAA

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=298, Percent_Identity=30.2013422818792, Blast_Score=101, Evalue=5e-23,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 31373; Mature: 31241

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGTNIKTYFDGAWHDADIPVMRAGDHGAWQGTTCFDGARYVDGMAPDLLAHCDRVVRS
CCCCCCEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHH
AKALSLNPGKTGQQIFDIVWEGLGRYPKDVAVYVRPNFFGIDGGQYGIIPATTEAGFFIC
HHHEECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCEEECCCCCEEEEECCCCCCEEEE
LEEVPMAAPDAASRLATTRFHRPVLNTAVLDAKAGCLYPNNARMLMEARDKGYDNALVTD
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEECCCCCCCEEHHH
AMGNVAETATANIFMVRDGEAFTPIPNGTFLNGITRQRHIANLRADGVTVTEAVLTFQDF
HHCCHHHHCEEEEEEEECCCEECCCCCCCEECCHHHHHHHHHHCCCCEEHHHHHHHHHHH
READEVFMTGNLNKVTPVLEFDGTHYQHGPVTKRARDLYWDWAASAA
HHHCCEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ATGTNIKTYFDGAWHDADIPVMRAGDHGAWQGTTCFDGARYVDGMAPDLLAHCDRVVRS
CCCCCEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHH
AKALSLNPGKTGQQIFDIVWEGLGRYPKDVAVYVRPNFFGIDGGQYGIIPATTEAGFFIC
HHHEECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCEEECCCCCEEEEECCCCCCEEEE
LEEVPMAAPDAASRLATTRFHRPVLNTAVLDAKAGCLYPNNARMLMEARDKGYDNALVTD
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEECCCCCCCEEHHH
AMGNVAETATANIFMVRDGEAFTPIPNGTFLNGITRQRHIANLRADGVTVTEAVLTFQDF
HHCCHHHHCEEEEEEEECCCEECCCCCCCEECCHHHHHHHHHHCCCCEEHHHHHHHHHHH
READEVFMTGNLNKVTPVLEFDGTHYQHGPVTKRARDLYWDWAASAA
HHHCCEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]