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The map label for this gene is plsB [H]

Identifier: 89053286

GI number: 89053286

Start: 755377

End: 756864

Strand: Reverse

Name: plsB [H]

Synonym: Jann_0795

Alternate gene names: 89053286

Gene position: 756864-755377 (Counterclockwise)

Preceding gene: 89053292

Following gene: 89053285

Centisome position: 17.53

GC content: 65.59

Gene sequence:

>1488_bases
GTGGGGCGATGCTCGCATTTTCGTCAGAAAACGCGGGCCAGCGTCACCCCCGACCCGCCCGATTGCCACGCCCCCCAATC
CCTGCCACCCTGCTGCCCCATGACCGCCCCCATCACGCTCCCCCTCTGGCTCTTTCTGCTGATTCTCGTCTTCGCCGCGG
TCACCTTCGCGTCGCACTTCCTGTTTCCCTCTGTGCGCTGGTTCCTGCGCCGCCGTCTGGAAACCGCCGTGGCGCGCCTG
AACAAGCGCCTGAAAACCCCGATCCAGCCGTTCAAGCTGGCCCGTCGCATGGATACCGTGCGCCGCCTTGTCTACGACCC
CCAGGTCGCCCAGGCGATCTCGGAACATGCGCGGTCCAAGGGCGTGCGCGAGGATGTCGCCGCCCAAACCGCCGAACGCT
ACGCCCGCGAAATCGTGCCCGGGTTTTCGGCCTTCACCTATTTCTCCTTCGCCATGCGCGCCTCTCGCATCCTGTCGCAA
TCGCTCTACCGCGTGCGGTTGACCAATCCCAACAACGATGCGCTGGAGGCAATTCCAGACGACGACACCGTGATTTTCGT
GATGAACCACCGCTCCAACATGGACTACGTGCTGGTCACCTGGCTGGTCGCCGAACGCTCCGCGCTGGCCTATGCCGTCG
GCGAATGGGCCCGCGTCTGGCCCCTGCGCGGGCTCGTGCGCGCGTTGGGCGGCTACTTCATCCGGCGGCGCCACAACAAC
CCGCTCTACCGCAAAGTCCTCGCGCGCTACGTGCAAATGGCGACCGAGGCGGGGCTGACACAAGCCGTCTTTCCAGAAGG
CGGGCTGTCGCGCACGGGCGGTCTGGGCAAGCCGAAACTGGGCCTTATCTCCTACATCCTTGATGGGTTCGAGCCCGGCA
CATCGCGGAATGTCACCTTCGTGCCCGTGGCCCTCAACTATGACCGCGTGATGGAGGATCGGAACCTGATCGCGGCCCAA
AAGGGCGGCACCGGCGGCTTCCGCTTTTCCCTCCTGCCCATCTACCGCTACCTGCGCCGCCAGATCTGGCATCGCATCAC
CGGCAAGTTCCATCGCTATGGCTATGCCGCCGTGTCCTTTGGCGATCCGCTCTCCCTCTCGGCCTTCCTCACCGCCAAAC
ACACCGACAATGCCGAGGCGTTGGGGGAGGCGTTGATGTCCCGTATTTCCGCCGTCACACCTGTTGTCCCCGTCCCCCTC
ATCGCCCACGCCCTGCGCGACGGGGCACGGACGACAGACACGCTGAAGGCGGCGGTTGGGTCCCGGATCGCACTGGCCGA
AGCCAAAGGCATCGGCGTCCACCTCGCCCGCGACGATCTGGACTACACCATCGAAGCCGGCCTGCGCGCCCTGATGGAAC
GCGGGTTTGTGGAACGCGACGGCGACGCCCTCACCGTCACCGAACGCGGGGTGTCTGTGGTGGATTACTACGCCGCAAGC
GTGGCGCATCTGCTGGACACAGAGGCGCTGGAGGGCGCTGCGGAGTGA

Upstream 100 bases:

>100_bases
TTCAGAAGTGTAAATTTTTATTGTTTCCAGTCCGTGCAGAATGGGGCGACGGCACCTTCGCTGTTTCATGAGCCGCTAAA
CACGCCCTGCGGGACGGCGG

Downstream 100 bases:

>100_bases
AGGTTCACGCAAGCGCAGCCAATCACATGCATATTTCGGGGGTCAGCCGTTACAGTCAGGTCATGGGCCTCCTCATGGGC
TGTATCGGAGCGTTTTTTCT

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MGRCSHFRQKTRASVTPDPPDCHAPQSLPPCCPMTAPITLPLWLFLLILVFAAVTFASHFLFPSVRWFLRRRLETAVARL
NKRLKTPIQPFKLARRMDTVRRLVYDPQVAQAISEHARSKGVREDVAAQTAERYAREIVPGFSAFTYFSFAMRASRILSQ
SLYRVRLTNPNNDALEAIPDDDTVIFVMNHRSNMDYVLVTWLVAERSALAYAVGEWARVWPLRGLVRALGGYFIRRRHNN
PLYRKVLARYVQMATEAGLTQAVFPEGGLSRTGGLGKPKLGLISYILDGFEPGTSRNVTFVPVALNYDRVMEDRNLIAAQ
KGGTGGFRFSLLPIYRYLRRQIWHRITGKFHRYGYAAVSFGDPLSLSAFLTAKHTDNAEALGEALMSRISAVTPVVPVPL
IAHALRDGARTTDTLKAAVGSRIALAEAKGIGVHLARDDLDYTIEAGLRALMERGFVERDGDALTVTERGVSVVDYYAAS
VAHLLDTEALEGAAE

Sequences:

>Translated_495_residues
MGRCSHFRQKTRASVTPDPPDCHAPQSLPPCCPMTAPITLPLWLFLLILVFAAVTFASHFLFPSVRWFLRRRLETAVARL
NKRLKTPIQPFKLARRMDTVRRLVYDPQVAQAISEHARSKGVREDVAAQTAERYAREIVPGFSAFTYFSFAMRASRILSQ
SLYRVRLTNPNNDALEAIPDDDTVIFVMNHRSNMDYVLVTWLVAERSALAYAVGEWARVWPLRGLVRALGGYFIRRRHNN
PLYRKVLARYVQMATEAGLTQAVFPEGGLSRTGGLGKPKLGLISYILDGFEPGTSRNVTFVPVALNYDRVMEDRNLIAAQ
KGGTGGFRFSLLPIYRYLRRQIWHRITGKFHRYGYAAVSFGDPLSLSAFLTAKHTDNAEALGEALMSRISAVTPVVPVPL
IAHALRDGARTTDTLKAAVGSRIALAEAKGIGVHLARDDLDYTIEAGLRALMERGFVERDGDALTVTERGVSVVDYYAAS
VAHLLDTEALEGAAE
>Mature_494_residues
GRCSHFRQKTRASVTPDPPDCHAPQSLPPCCPMTAPITLPLWLFLLILVFAAVTFASHFLFPSVRWFLRRRLETAVARLN
KRLKTPIQPFKLARRMDTVRRLVYDPQVAQAISEHARSKGVREDVAAQTAERYAREIVPGFSAFTYFSFAMRASRILSQS
LYRVRLTNPNNDALEAIPDDDTVIFVMNHRSNMDYVLVTWLVAERSALAYAVGEWARVWPLRGLVRALGGYFIRRRHNNP
LYRKVLARYVQMATEAGLTQAVFPEGGLSRTGGLGKPKLGLISYILDGFEPGTSRNVTFVPVALNYDRVMEDRNLIAAQK
GGTGGFRFSLLPIYRYLRRQIWHRITGKFHRYGYAAVSFGDPLSLSAFLTAKHTDNAEALGEALMSRISAVTPVVPVPLI
AHALRDGARTTDTLKAAVGSRIALAEAKGIGVHLARDDLDYTIEAGLRALMERGFVERDGDALTVTERGVSVVDYYAASV
AHLLDTEALEGAAE

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family [H]

Homologues:

Organism=Homo sapiens, GI190358539, Length=223, Percent_Identity=26.0089686098655, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI87082362, Length=311, Percent_Identity=28.6173633440514, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25147672, Length=286, Percent_Identity=23.0769230769231, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71988723, Length=280, Percent_Identity=25, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71988728, Length=196, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24650754, Length=201, Percent_Identity=31.8407960199005, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24650752, Length=201, Percent_Identity=31.8407960199005, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21357731, Length=201, Percent_Identity=31.8407960199005, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002123
- InterPro:   IPR022284 [H]

Pfam domain/function: PF01553 Acyltransferase [H]

EC number: =2.3.1.15 [H]

Molecular weight: Translated: 55091; Mature: 54960

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRCSHFRQKTRASVTPDPPDCHAPQSLPPCCPMTAPITLPLWLFLLILVFAAVTFASHF
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LFPSVRWFLRRRLETAVARLNKRLKTPIQPFKLARRMDTVRRLVYDPQVAQAISEHARSK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
GVREDVAAQTAERYAREIVPGFSAFTYFSFAMRASRILSQSLYRVRLTNPNNDALEAIPD
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCC
DDTVIFVMNHRSNMDYVLVTWLVAERSALAYAVGEWARVWPLRGLVRALGGYFIRRRHNN
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLYRKVLARYVQMATEAGLTQAVFPEGGLSRTGGLGKPKLGLISYILDGFEPGTSRNVTF
HHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEE
VPVALNYDRVMEDRNLIAAQKGGTGGFRFSLLPIYRYLRRQIWHRITGKFHRYGYAAVSF
EEEEECHHHHHCCCCEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
GDPLSLSAFLTAKHTDNAEALGEALMSRISAVTPVVPVPLIAHALRDGARTTDTLKAAVG
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHC
SRIALAEAKGIGVHLARDDLDYTIEAGLRALMERGFVERDGDALTVTERGVSVVDYYAAS
CCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHH
VAHLLDTEALEGAAE
HHHHHHHHHHCCCCC
>Mature Secondary Structure 
GRCSHFRQKTRASVTPDPPDCHAPQSLPPCCPMTAPITLPLWLFLLILVFAAVTFASHF
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LFPSVRWFLRRRLETAVARLNKRLKTPIQPFKLARRMDTVRRLVYDPQVAQAISEHARSK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
GVREDVAAQTAERYAREIVPGFSAFTYFSFAMRASRILSQSLYRVRLTNPNNDALEAIPD
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCC
DDTVIFVMNHRSNMDYVLVTWLVAERSALAYAVGEWARVWPLRGLVRALGGYFIRRRHNN
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLYRKVLARYVQMATEAGLTQAVFPEGGLSRTGGLGKPKLGLISYILDGFEPGTSRNVTF
HHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEE
VPVALNYDRVMEDRNLIAAQKGGTGGFRFSLLPIYRYLRRQIWHRITGKFHRYGYAAVSF
EEEEECHHHHHCCCCEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
GDPLSLSAFLTAKHTDNAEALGEALMSRISAVTPVVPVPLIAHALRDGARTTDTLKAAVG
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHC
SRIALAEAKGIGVHLARDDLDYTIEAGLRALMERGFVERDGDALTVTERGVSVVDYYAAS
CCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHH
VAHLLDTEALEGAAE
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA