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The map label for this gene is pdxJ

Identifier: 89053007

GI number: 89053007

Start: 510374

End: 511120

Strand: Direct

Name: pdxJ

Synonym: Jann_0516

Alternate gene names: 89053007

Gene position: 510374-511120 (Clockwise)

Preceding gene: 89053006

Following gene: 89053008

Centisome position: 11.82

GC content: 64.79

Gene sequence:

>747_bases
ATGAGCGCCATGGAGAAGCTGAGACTGGGTGTGAACATCGACCACGTCGCAACCCTGCGCAATGCGCGAGGCGGGGCACT
GCCCGATCCGGTGCGCGCGGCCGTTCTGGCAGAACAGGCCGGGGCCGACGGGATCACGGCGCATCTGCGGGAAGACCGAC
GCCATATCCGGGACGCCGACATTGCCGCGATCATGGAGGCGATCACCATCCCGCTGAATTTCGAAATGGCCGCGACCGAC
GAGATGCAGGAGATCGCGTTGGGCCACCTGCCCCACGCCGCCTGCATCGTGCCCGAACGGCGGGAGGAGCGGACGACAGA
GGGCGGGTTGGAAGTGGCGGGTGACGACAATCGCCTCGCGCAATACATCGCGCCGTTGAAGGATGCGGGCATACGTGTGT
CGCTGTTCATCGCGGCGGAGCGGTCGCAGATTGAAGCCGCTGCGCGCATCGGTGCCCCGGTCATCGAACTGCACACCGGC
GCCTATTGTGACTATGACGCCGAGGGTGATGTTGCAGCGCGGGATGCGGAGCTGGCGCGGTTGATCGCGGGTGCGAAGCT
GGGGGTTGAGTTGGGGTTGGAGGTTCATATGGGCCACGGCCTGAACTACGACACGGTCGCCCCCATCGCAGCGCTTCCCG
AAGTGGCCGAGCTGAACATTGGCCACTTTCTGATTGGCGAATCGATCTTTGTCGGGCTTGAGGCTGCCATGACCGAGATG
CGTGCCCGTATGGATGCGGCGCGGTGA

Upstream 100 bases:

>100_bases
GGGCCTACCAGAAAAGCCGTGCAGCCCGATTGCGGAAGAAGATGGACAAGCTGCGCAAGCAAGCGGAAGCGGCGGAGTGA
ACCCGCCCGACGGTGAAAGG

Downstream 100 bases:

>100_bases
TGGCCGTTAACCTCAAACGCTATGGGTTGGTGTATCTTGGTACGATCGTCGGGCTTGTGGGGCTGTCGAACGCGATGGTG
TACTTTCTTGACATGTCATT

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MSAMEKLRLGVNIDHVATLRNARGGALPDPVRAAVLAEQAGADGITAHLREDRRHIRDADIAAIMEAITIPLNFEMAATD
EMQEIALGHLPHAACIVPERREERTTEGGLEVAGDDNRLAQYIAPLKDAGIRVSLFIAAERSQIEAAARIGAPVIELHTG
AYCDYDAEGDVAARDAELARLIAGAKLGVELGLEVHMGHGLNYDTVAPIAALPEVAELNIGHFLIGESIFVGLEAAMTEM
RARMDAAR

Sequences:

>Translated_248_residues
MSAMEKLRLGVNIDHVATLRNARGGALPDPVRAAVLAEQAGADGITAHLREDRRHIRDADIAAIMEAITIPLNFEMAATD
EMQEIALGHLPHAACIVPERREERTTEGGLEVAGDDNRLAQYIAPLKDAGIRVSLFIAAERSQIEAAARIGAPVIELHTG
AYCDYDAEGDVAARDAELARLIAGAKLGVELGLEVHMGHGLNYDTVAPIAALPEVAELNIGHFLIGESIFVGLEAAMTEM
RARMDAAR
>Mature_247_residues
SAMEKLRLGVNIDHVATLRNARGGALPDPVRAAVLAEQAGADGITAHLREDRRHIRDADIAAIMEAITIPLNFEMAATDE
MQEIALGHLPHAACIVPERREERTTEGGLEVAGDDNRLAQYIAPLKDAGIRVSLFIAAERSQIEAAARIGAPVIELHTGA
YCDYDAEGDVAARDAELARLIAGAKLGVELGLEVHMGHGLNYDTVAPIAALPEVAELNIGHFLIGESIFVGLEAAMTEMR
ARMDAAR

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=245, Percent_Identity=54.6938775510204, Blast_Score=258, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_JANSC (Q28V29)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508458.1
- ProteinModelPortal:   Q28V29
- SMR:   Q28V29
- STRING:   Q28V29
- GeneID:   3932957
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0516
- eggNOG:   COG0854
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- PhylomeDB:   Q28V29
- ProtClustDB:   PRK05265
- BioCyc:   JSP290400:JANN_0516-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 26494; Mature: 26362

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: ACT_SITE 48-48 ACT_SITE 75-75 ACT_SITE 199-199 BINDING 12-12 BINDING 23-23 BINDING 50-50 BINDING 55-55 BINDING 105-105 BINDING 200-200

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAMEKLRLGVNIDHVATLRNARGGALPDPVRAAVLAEQAGADGITAHLREDRRHIRDAD
CCHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHH
IAAIMEAITIPLNFEMAATDEMQEIALGHLPHAACIVPERREERTTEGGLEVAGDDNRLA
HHHHHHHHHCCCCEEECCHHHHHHHHHHCCCCCEEECCCHHHHCCCCCCEEEECCCHHHH
QYIAPLKDAGIRVSLFIAAERSQIEAAARIGAPVIELHTGAYCDYDAEGDVAARDAELAR
HHHHHHHCCCEEEEEEEEECHHHHHHHHHCCCCEEEECCCCEECCCCCCCCCHHHHHHHH
LIAGAKLGVELGLEVHMGHGLNYDTVAPIAALPEVAELNIGHFLIGESIFVGLEAAMTEM
HHHCCCCCEEECEEEEECCCCCCCCCCHHHHCCHHHHCCCCEEECCCHHHHHHHHHHHHH
RARMDAAR
HHHHHCCC
>Mature Secondary Structure 
SAMEKLRLGVNIDHVATLRNARGGALPDPVRAAVLAEQAGADGITAHLREDRRHIRDAD
CHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHH
IAAIMEAITIPLNFEMAATDEMQEIALGHLPHAACIVPERREERTTEGGLEVAGDDNRLA
HHHHHHHHHCCCCEEECCHHHHHHHHHHCCCCCEEECCCHHHHCCCCCCEEEECCCHHHH
QYIAPLKDAGIRVSLFIAAERSQIEAAARIGAPVIELHTGAYCDYDAEGDVAARDAELAR
HHHHHHHCCCEEEEEEEEECHHHHHHHHHCCCCEEEECCCCEECCCCCCCCCHHHHHHHH
LIAGAKLGVELGLEVHMGHGLNYDTVAPIAALPEVAELNIGHFLIGESIFVGLEAAMTEM
HHHCCCCCEEECEEEEECCCCCCCCCCHHHHCCHHHHCCCCEEECCCHHHHHHHHHHHHH
RARMDAAR
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA