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The map label for this gene is gcvA [H]

Identifier: 89052982

GI number: 89052982

Start: 488947

End: 489894

Strand: Reverse

Name: gcvA [H]

Synonym: Jann_0491

Alternate gene names: 89052982

Gene position: 489894-488947 (Counterclockwise)

Preceding gene: 89052983

Following gene: 89052978

Centisome position: 11.35

GC content: 63.61

Gene sequence:

>948_bases
ATGTCTGATCGTCTCCCTCCCCTCACCGCGCTGCGCGCCTTTGATGCCGCTGCTCGGCACATGTCCTTCTCCAAAGCTGC
GGCTGAGTTGAATGTCACGCCCGCCGCCCTGTCGTTTCAGATCAAATCGCTGGAGGAGCATTTGGAAGCGCCGTTGTTCC
GTCGCCTCAACCGCGCGGTGGAACTGACCGATGCGGGCAAAGCCCTCGCACCCGGGGCGTCCGACGGGTTCACCGCGCTG
GCCAGTGCCTGGCGCGCGGCCAACCGCACGGTGGATCAGACCAGCCTGACCGTCACCGCGGGGCCCGCCTTCACCGCAAA
ATGGCTCGCACCCCGGCTGTTCAAGTTCGCCATGGCCAATCCGGAGATCGAGCTTCGGTTCTCGGCCACCCTGCGCCTGA
TGGATTTCGCACGCGATGACGTGGACGTGGCGATCCGGTTCAGCTTGGCCAAGGAAGATCCCAACCTCGATTCACGGCCC
ATCATCCGCGAATGGGTTACGCCGATGATGACGCCGGAGCTGGCCGAGGCCTATCCAGACCCAACAGACCTCGCCCGCGC
GCCGCTGTTGCATCAGGATGATATTCAGTTCCTGAAGCCTGCCGTTGACTGGCCCGCGTGGTTCCGGGCCGCACAATTGC
CGCCTCGTTTTGCAGCCGGCGCACGGTTCAGCCAGGCGGATCACGCCATTGATGCGGCCTTGGCCGGGGCGGGGGTCGTG
TTGGGGCGTATCTCTCTGGCCGAAAAGGACCTGAAAGAGGGGCGTCTGGTCATGCCTTACAAGATCGCGCTGGCGACCGA
GGCAAAGTATAGCTTCGTCTGCCCGGAAGGATCAGATGCGCGGCCACAGGTGCGGGCGTTCCGTGAGTGGATTGAGGCAG
AAACAGCCACGCTTGATGCCTACCGCGAAGGGCTGACATTCGTGAACGCCGCCGAGGTTGAGCAATAG

Upstream 100 bases:

>100_bases
TTTGATGCGTATGCTTGCTGTTGATCTGAATATGGGCCGTCATCCGAGATCCCGCAAACGAGACTTTCCAACCACTCACA
TAAGGTATACTTAACTGATT

Downstream 100 bases:

>100_bases
GCTCAAAGCCCGACGACGCAAAGTTTGCGCGCCACATCACTGGATCCGGTCTAACGCGGCCTGGGTGGCGCTGAAAAGCT
AGCTGTCGAGCCGTGCGATT

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSDRLPPLTALRAFDAAARHMSFSKAAAELNVTPAALSFQIKSLEEHLEAPLFRRLNRAVELTDAGKALAPGASDGFTAL
ASAWRAANRTVDQTSLTVTAGPAFTAKWLAPRLFKFAMANPEIELRFSATLRLMDFARDDVDVAIRFSLAKEDPNLDSRP
IIREWVTPMMTPELAEAYPDPTDLARAPLLHQDDIQFLKPAVDWPAWFRAAQLPPRFAAGARFSQADHAIDAALAGAGVV
LGRISLAEKDLKEGRLVMPYKIALATEAKYSFVCPEGSDARPQVRAFREWIEAETATLDAYREGLTFVNAAEVEQ

Sequences:

>Translated_315_residues
MSDRLPPLTALRAFDAAARHMSFSKAAAELNVTPAALSFQIKSLEEHLEAPLFRRLNRAVELTDAGKALAPGASDGFTAL
ASAWRAANRTVDQTSLTVTAGPAFTAKWLAPRLFKFAMANPEIELRFSATLRLMDFARDDVDVAIRFSLAKEDPNLDSRP
IIREWVTPMMTPELAEAYPDPTDLARAPLLHQDDIQFLKPAVDWPAWFRAAQLPPRFAAGARFSQADHAIDAALAGAGVV
LGRISLAEKDLKEGRLVMPYKIALATEAKYSFVCPEGSDARPQVRAFREWIEAETATLDAYREGLTFVNAAEVEQ
>Mature_314_residues
SDRLPPLTALRAFDAAARHMSFSKAAAELNVTPAALSFQIKSLEEHLEAPLFRRLNRAVELTDAGKALAPGASDGFTALA
SAWRAANRTVDQTSLTVTAGPAFTAKWLAPRLFKFAMANPEIELRFSATLRLMDFARDDVDVAIRFSLAKEDPNLDSRPI
IREWVTPMMTPELAEAYPDPTDLARAPLLHQDDIQFLKPAVDWPAWFRAAQLPPRFAAGARFSQADHAIDAALAGAGVVL
GRISLAEKDLKEGRLVMPYKIALATEAKYSFVCPEGSDARPQVRAFREWIEAETATLDAYREGLTFVNAAEVEQ

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=300, Percent_Identity=38.3333333333333, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=35.6164383561644, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1788706, Length=303, Percent_Identity=29.7029702970297, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI157672245, Length=190, Percent_Identity=33.1578947368421, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=26.3513513513513, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787128, Length=250, Percent_Identity=27.6, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081978, Length=195, Percent_Identity=31.7948717948718, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34609; Mature: 34478

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRLPPLTALRAFDAAARHMSFSKAAAELNVTPAALSFQIKSLEEHLEAPLFRRLNRAV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHE
ELTDAGKALAPGASDGFTALASAWRAANRTVDQTSLTVTAGPAFTAKWLAPRLFKFAMAN
EECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCC
PEIELRFSATLRLMDFARDDVDVAIRFSLAKEDPNLDSRPIIREWVTPMMTPELAEAYPD
CCEEEEEHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHCCCHHHHHHCCC
PTDLARAPLLHQDDIQFLKPAVDWPAWFRAAQLPPRFAAGARFSQADHAIDAALAGAGVV
HHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHH
LGRISLAEKDLKEGRLVMPYKIALATEAKYSFVCPEGSDARPQVRAFREWIEAETATLDA
HHHHHHHHHHHHCCCEEEEEEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
YREGLTFVNAAEVEQ
HHHCCCEECHHCCCC
>Mature Secondary Structure 
SDRLPPLTALRAFDAAARHMSFSKAAAELNVTPAALSFQIKSLEEHLEAPLFRRLNRAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHE
ELTDAGKALAPGASDGFTALASAWRAANRTVDQTSLTVTAGPAFTAKWLAPRLFKFAMAN
EECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCC
PEIELRFSATLRLMDFARDDVDVAIRFSLAKEDPNLDSRPIIREWVTPMMTPELAEAYPD
CCEEEEEHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHCCCHHHHHHCCC
PTDLARAPLLHQDDIQFLKPAVDWPAWFRAAQLPPRFAAGARFSQADHAIDAALAGAGVV
HHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHH
LGRISLAEKDLKEGRLVMPYKIALATEAKYSFVCPEGSDARPQVRAFREWIEAETATLDA
HHHHHHHHHHHHCCCEEEEEEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
YREGLTFVNAAEVEQ
HHHCCCEECHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]