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The map label for this gene is ispB [H]

Identifier: 89052978

GI number: 89052978

Start: 486583

End: 487590

Strand: Reverse

Name: ispB [H]

Synonym: Jann_0487

Alternate gene names: 89052978

Gene position: 487590-486583 (Counterclockwise)

Preceding gene: 89052982

Following gene: 89052974

Centisome position: 11.29

GC content: 63.59

Gene sequence:

>1008_bases
ATGAGTTTGGACACGCCAACGCAGAAGCCCCACGACCGCCTGAGCGCTGCACTGAAAGATGATCTGACGGCCGTGAACAC
GCTGATCCGCGCGCGGATGGCTTCCAAACACGCCCCTCGCATTCCAGAGGTCACGGCACATCTGGTGGAGGCGGGCGGCA
AGCGCATCCGGCCCATGCTAACGCTGGCCTGCGCGCGGCTGTGTGGCTACTCCGGCGATGATCACGTGAAACTGGCCGCC
ACGGTAGAGTTCATTCACACCGCGACCCTGCTGCACGACGATGTGGTCGATGAAAGCGAGCAACGCCGGGGCCGCCCCAC
GGCGAACCTGTTGTGGGACAATCAATCCAGCGTGCTGGTGGGCGATTACCTGTTCGCCCGCGCGTTCCAGTTGATGGTGG
AGCCCGGCAACCTGCGCGTCCTCGACATCCTGTCAAACGCCGCCGCCACCATTGCCGAAGGCGAAGTGCTGCAACTCACC
GTCGCCTCCAACATCGAAACGCCTGAGGAGACGTATCTTCAGGTCATCCGGGGCAAAACCGCCGCGCTGTTTGAAGCTGC
CTGCGAAGTGGGCGGTGTTATCGCCGGGCGTGACACGTTCATTATCCAGGCCCTCGCCACCTATGGCGACGGGTTGGGCG
TGGCCTTCCAGATGGCCGATGACCTTCTGGACTGGGGCGGCGTGGATGGCGGGGCACAACTGGACAAGAACATCGGCGAT
GACTTCCGGGAGCGGAAGATGACCCTGCCCGTGATCCGGGCCATCGCGGCGGCAAACGGTGATGAACGCGCCTTCTGGGC
GCGCACCATCGGCAAGGGCGATCAACGCGACGGCGATCTTGAACAGGCGCTTGATGTGTTGCAACGCCATGGCACCCTCG
CCAGCACGCGTGACGCGGCCAATGACCACGTGGCACGCGCCAAAGCCGCCCTTACAAACCTGCCCGACGATCCCCTTCGC
GACATGCTGGCCGATCTGGCAGATTACGTGGTGGCGCGCCTGAATTAG

Upstream 100 bases:

>100_bases
GTGGCGAAGGCGATAATTGTGGGATCGTTGCTGCGCAGAATTTCAAGCATAACTGACAGGTAATCACGCCTGCGCAACAT
GTCGAGATGGGGATAGGGAT

Downstream 100 bases:

>100_bases
GCCCGCGCTTTCAGCATCCGTAGGGTGGGATTTACCCCGCCGGGGGCTATGCCCTCAAGACGGGCGCGTAGGTCACCCAC
CAGTGCTTGGGCAGACCCAG

Product: trans-hexaprenyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSLDTPTQKPHDRLSAALKDDLTAVNTLIRARMASKHAPRIPEVTAHLVEAGGKRIRPMLTLACARLCGYSGDDHVKLAA
TVEFIHTATLLHDDVVDESEQRRGRPTANLLWDNQSSVLVGDYLFARAFQLMVEPGNLRVLDILSNAAATIAEGEVLQLT
VASNIETPEETYLQVIRGKTAALFEAACEVGGVIAGRDTFIIQALATYGDGLGVAFQMADDLLDWGGVDGGAQLDKNIGD
DFRERKMTLPVIRAIAAANGDERAFWARTIGKGDQRDGDLEQALDVLQRHGTLASTRDAANDHVARAKAALTNLPDDPLR
DMLADLADYVVARLN

Sequences:

>Translated_335_residues
MSLDTPTQKPHDRLSAALKDDLTAVNTLIRARMASKHAPRIPEVTAHLVEAGGKRIRPMLTLACARLCGYSGDDHVKLAA
TVEFIHTATLLHDDVVDESEQRRGRPTANLLWDNQSSVLVGDYLFARAFQLMVEPGNLRVLDILSNAAATIAEGEVLQLT
VASNIETPEETYLQVIRGKTAALFEAACEVGGVIAGRDTFIIQALATYGDGLGVAFQMADDLLDWGGVDGGAQLDKNIGD
DFRERKMTLPVIRAIAAANGDERAFWARTIGKGDQRDGDLEQALDVLQRHGTLASTRDAANDHVARAKAALTNLPDDPLR
DMLADLADYVVARLN
>Mature_334_residues
SLDTPTQKPHDRLSAALKDDLTAVNTLIRARMASKHAPRIPEVTAHLVEAGGKRIRPMLTLACARLCGYSGDDHVKLAAT
VEFIHTATLLHDDVVDESEQRRGRPTANLLWDNQSSVLVGDYLFARAFQLMVEPGNLRVLDILSNAAATIAEGEVLQLTV
ASNIETPEETYLQVIRGKTAALFEAACEVGGVIAGRDTFIIQALATYGDGLGVAFQMADDLLDWGGVDGGAQLDKNIGDD
FRERKMTLPVIRAIAAANGDERAFWARTIGKGDQRDGDLEQALDVLQRHGTLASTRDAANDHVARAKAALTNLPDDPLRD
MLADLADYVVARLN

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=300, Percent_Identity=28.3333333333333, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI1789578, Length=322, Percent_Identity=44.0993788819876, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI1786623, Length=274, Percent_Identity=30.2919708029197, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=303, Percent_Identity=27.0627062706271, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6319475, Length=302, Percent_Identity=29.8013245033113, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6325188, Length=307, Percent_Identity=23.1270358306189, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24651612, Length=330, Percent_Identity=28.4848484848485, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI281365769, Length=284, Percent_Identity=21.830985915493, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24660002, Length=284, Percent_Identity=21.830985915493, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36243; Mature: 36112

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDTPTQKPHDRLSAALKDDLTAVNTLIRARMASKHAPRIPEVTAHLVEAGGKRIRPML
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHH
TLACARLCGYSGDDHVKLAATVEFIHTATLLHDDVVDESEQRRGRPTANLLWDNQSSVLV
HHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEECCCCCEEH
GDYLFARAFQLMVEPGNLRVLDILSNAAATIAEGEVLQLTVASNIETPEETYLQVIRGKT
HHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCH
AALFEAACEVGGVIAGRDTFIIQALATYGDGLGVAFQMADDLLDWGGVDGGAQLDKNIGD
HHHHHHHHHHCCEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCH
DFRERKMTLPVIRAIAAANGDERAFWARTIGKGDQRDGDLEQALDVLQRHGTLASTRDAA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHH
NDHVARAKAALTNLPDDPLRDMLADLADYVVARLN
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLDTPTQKPHDRLSAALKDDLTAVNTLIRARMASKHAPRIPEVTAHLVEAGGKRIRPML
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHH
TLACARLCGYSGDDHVKLAATVEFIHTATLLHDDVVDESEQRRGRPTANLLWDNQSSVLV
HHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEECCCCCEEH
GDYLFARAFQLMVEPGNLRVLDILSNAAATIAEGEVLQLTVASNIETPEETYLQVIRGKT
HHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCH
AALFEAACEVGGVIAGRDTFIIQALATYGDGLGVAFQMADDLLDWGGVDGGAQLDKNIGD
HHHHHHHHHHCCEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCH
DFRERKMTLPVIRAIAAANGDERAFWARTIGKGDQRDGDLEQALDVLQRHGTLASTRDAA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHH
NDHVARAKAALTNLPDDPLRDMLADLADYVVARLN
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]