Click here to switch to the map view.

The map label for this gene is pheT

Identifier: 89052945

GI number: 89052945

Start: 455198

End: 457600

Strand: Direct

Name: pheT

Synonym: Jann_0454

Alternate gene names: 89052945

Gene position: 455198-457600 (Clockwise)

Preceding gene: 89052944

Following gene: 89052946

Centisome position: 10.54

GC content: 63.67

Gene sequence:

>2403_bases
ATGAAATTCACCCTCTCCTGGCTGAAAGATCACCTCGACACGGACGCCTCCGTCACTGAGATCACCGATGCCCTCACGGA
CCTCGGGCTTGAGGTTGAGGGTGTGGAGGATCGGGCGGAGGCGCTCAAGACGTTCACCATCGCCCACATCCAGTCTGCCG
AGCAGCACCCGGACGCGGATCGTCTGCGCATTTGTCAGGTGGAGACCAACGATGGCCTCCAACAGATCATCTGCGGTGCG
CCTAATGCGCGGGCGGGGATCAACGTGGTGCTGGCCAAGCCTGGCGATTATATCCCCGGCCTCGATATCACAATCGGCGT
TGGCAAAATCCGCGGCGTGGAGAGCTTTGGCATGATGGCTTCTGAGAAGGAGCTGCTGCTGTCGGACGAGCACAACGGCA
TCATCGAGTTGAAAGACGCGCAGGTCGGCCAGAAGTTCATCGATTGGCTGGCCAAGCATGACCCCGCCAAAGTCGACCCG
GTGATCGAGATCGCGATCACGCCGAACCGCCAAGACGCGTTGGGCATTCGCGGCATCGCACGGGACCTGTCCGCGCGCGG
TGTCGGCACGTTGAAGGCCCATGATCCGGCGGAGATCAAGGGTACGTATCCGTCCCCGATCAGCATTACGATTGACGCCG
ACACGCTGGACACCGCGCCGCATTTCACCGGGCGTTTGATCAAGGGCGTCAAGAACGGCCCCAGCCCCGCGTGGCTGCAG
GACCGTCTACGGGCCATCGGATTGCGTCCGATTTCCGCGCTGGTGGACATCACCAACTACTTCACCTTCGACCAAAACCG
CCCCCTTCACGTCTTCGACGCGGATAAGGTGGCCGGCGGCCTGCGCGTGCACAAAGCGGCGGGCGGCGAAGAGATCGTGG
CGCTGGATGACAAGACTTACACGTTGCCTGAGGGCCATATGGTGATCAGCGACGACACGGGTGTGGAAAGCATCGCGGGC
ATCATGGGCGGCGCGGCGACGGGCTGCACGGAGGACACGGTCAACGTGTTCCTGGAGAGCGCGTGGTGGGATACGGTCGC
GATTGCCTATGCGGGGCGCGCGTTGAAGATCAACTCCGACGCGCGCTATCGGTTCGAGCGGGGGGTGGACCCGGACTATA
CGCTGCCCGGCTTGCACGCCGCGACGCAGATGGTGCTGGACCTGTGCGGTGGTGAGGCGTCTGAGGTTGTCGAAGCTGGC
AAGCCACTGGACACGGCACGCAGTTATACGCTGCGGCCGGGTCGCGTGAAATCGCTCGTTGGCATGGACGTCGCCCGCGA
GGAACAGGCCCGGATCCTGACCGCGCTGGGGTTCTCGGCCACGGGCACCGATACGCTGGAGGTCTGGGTGCCAAGCTGGC
GCCGCGATGTGCAGGGGGAGGCTGATCTGGTTGAAGAAGTGGCCCGCGTGACGTCCCTATCGCAGTTGAAACCGCAACCC
TTGCCGCGCGAACCTGGCGTCCCTGCGCCTGTCCTGACGCCGATGCAAAAGCGCGAACGCAGCGCCCGGCGAACGATGGC
CGCGCTCGGTTACAATGAATGCGTGACCTATTCCTTCATCGACAAGGCGAGTGCCGAGCTGTTCGGCGGCGGCACCGACG
CGGTCATGCTGGAAAACCCGATTTCCTCGGAGATGTCGCATATGCGCCCCGCCGTTCTGCCCGGCCTTCTGCAAGCCGCC
GCGCGCAATCAGGCGCGGGGCATGATGGATATGGCGCTGTTTGAAGTTGGCGCCGGGTTCCACGGTGGTGAGCCGGGAGA
GCAGCATTTGCTCGCCTCCGGTCTGCTGATCGGCCACACTGGCCCACGTGATCCCCATGGGGCGCGGCGGCCGGTCGATG
TCTATGACGTGAAAGCGGACGCGGAAGCGGTACTGTCTGCCATTGGCGCCCCCGCCAAGGCGCAAATCCTGCGAGGCGCG
CGTGAGTGGTGGCATCCTGGACGGCACGGGATGATCTGTCTTGGGCCCAAGAAAGTGCTTGGCATTTTCGGCGAAGTGCA
CCCGAAGGTTCTGGCGGCGATGGATATCAAAGGCCCCGCGATGGCCTTCACGATTTTCCCGCAAGAGGTGCCGCTGCCGC
GCAGCACCAACGCCAGCCGGGGGGCTGTCACGATGTCCGATTTGCAGGCGGTGGAGCGCGATTTCGCATTTGTCGTGCCA
AGCGACGTGCCCGCCCTAGATTTGGTCAATGCGGCGGCGGGGGCGGACAAGACGCTGATCGCAGATGTGCGCGTCTTTGA
CGAGTTCATCGGCGGGTCACTTGGGGAGGGTAATAAGTCCATCGCCCTGGCCGTGCGGATGCAACCGCAGGACAAGACGC
TGACCGAGGAAGAGATCGAGGCCGTGGCCGACAAGATTGTGACCAAGGTGTCCAAGGCGACGGGCGGTGTGCTGCGCGGC
TAA

Upstream 100 bases:

>100_bases
TGCGTGGAAACAAGTACGAACCAATATTCCAACTATTGCCGCGGCGGTCATAACTGCCTTGGCGATAAATTGGGCAATCA
AGTATTTCGGAGCCTAACAA

Downstream 100 bases:

>100_bases
AGCGCGACCCGAAACGTGGGAACCGGTTGTCGGGCTATGTTGCGCGGCAAAAAGAAGGAGAGATGCGGTGAAGGTATATC
TGTCGGGCGAGATTCACACC

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 800; Mature: 800

Protein sequence:

>800_residues
MKFTLSWLKDHLDTDASVTEITDALTDLGLEVEGVEDRAEALKTFTIAHIQSAEQHPDADRLRICQVETNDGLQQIICGA
PNARAGINVVLAKPGDYIPGLDITIGVGKIRGVESFGMMASEKELLLSDEHNGIIELKDAQVGQKFIDWLAKHDPAKVDP
VIEIAITPNRQDALGIRGIARDLSARGVGTLKAHDPAEIKGTYPSPISITIDADTLDTAPHFTGRLIKGVKNGPSPAWLQ
DRLRAIGLRPISALVDITNYFTFDQNRPLHVFDADKVAGGLRVHKAAGGEEIVALDDKTYTLPEGHMVISDDTGVESIAG
IMGGAATGCTEDTVNVFLESAWWDTVAIAYAGRALKINSDARYRFERGVDPDYTLPGLHAATQMVLDLCGGEASEVVEAG
KPLDTARSYTLRPGRVKSLVGMDVAREEQARILTALGFSATGTDTLEVWVPSWRRDVQGEADLVEEVARVTSLSQLKPQP
LPREPGVPAPVLTPMQKRERSARRTMAALGYNECVTYSFIDKASAELFGGGTDAVMLENPISSEMSHMRPAVLPGLLQAA
ARNQARGMMDMALFEVGAGFHGGEPGEQHLLASGLLIGHTGPRDPHGARRPVDVYDVKADAEAVLSAIGAPAKAQILRGA
REWWHPGRHGMICLGPKKVLGIFGEVHPKVLAAMDIKGPAMAFTIFPQEVPLPRSTNASRGAVTMSDLQAVERDFAFVVP
SDVPALDLVNAAAGADKTLIADVRVFDEFIGGSLGEGNKSIALAVRMQPQDKTLTEEEIEAVADKIVTKVSKATGGVLRG

Sequences:

>Translated_800_residues
MKFTLSWLKDHLDTDASVTEITDALTDLGLEVEGVEDRAEALKTFTIAHIQSAEQHPDADRLRICQVETNDGLQQIICGA
PNARAGINVVLAKPGDYIPGLDITIGVGKIRGVESFGMMASEKELLLSDEHNGIIELKDAQVGQKFIDWLAKHDPAKVDP
VIEIAITPNRQDALGIRGIARDLSARGVGTLKAHDPAEIKGTYPSPISITIDADTLDTAPHFTGRLIKGVKNGPSPAWLQ
DRLRAIGLRPISALVDITNYFTFDQNRPLHVFDADKVAGGLRVHKAAGGEEIVALDDKTYTLPEGHMVISDDTGVESIAG
IMGGAATGCTEDTVNVFLESAWWDTVAIAYAGRALKINSDARYRFERGVDPDYTLPGLHAATQMVLDLCGGEASEVVEAG
KPLDTARSYTLRPGRVKSLVGMDVAREEQARILTALGFSATGTDTLEVWVPSWRRDVQGEADLVEEVARVTSLSQLKPQP
LPREPGVPAPVLTPMQKRERSARRTMAALGYNECVTYSFIDKASAELFGGGTDAVMLENPISSEMSHMRPAVLPGLLQAA
ARNQARGMMDMALFEVGAGFHGGEPGEQHLLASGLLIGHTGPRDPHGARRPVDVYDVKADAEAVLSAIGAPAKAQILRGA
REWWHPGRHGMICLGPKKVLGIFGEVHPKVLAAMDIKGPAMAFTIFPQEVPLPRSTNASRGAVTMSDLQAVERDFAFVVP
SDVPALDLVNAAAGADKTLIADVRVFDEFIGGSLGEGNKSIALAVRMQPQDKTLTEEEIEAVADKIVTKVSKATGGVLRG
>Mature_800_residues
MKFTLSWLKDHLDTDASVTEITDALTDLGLEVEGVEDRAEALKTFTIAHIQSAEQHPDADRLRICQVETNDGLQQIICGA
PNARAGINVVLAKPGDYIPGLDITIGVGKIRGVESFGMMASEKELLLSDEHNGIIELKDAQVGQKFIDWLAKHDPAKVDP
VIEIAITPNRQDALGIRGIARDLSARGVGTLKAHDPAEIKGTYPSPISITIDADTLDTAPHFTGRLIKGVKNGPSPAWLQ
DRLRAIGLRPISALVDITNYFTFDQNRPLHVFDADKVAGGLRVHKAAGGEEIVALDDKTYTLPEGHMVISDDTGVESIAG
IMGGAATGCTEDTVNVFLESAWWDTVAIAYAGRALKINSDARYRFERGVDPDYTLPGLHAATQMVLDLCGGEASEVVEAG
KPLDTARSYTLRPGRVKSLVGMDVAREEQARILTALGFSATGTDTLEVWVPSWRRDVQGEADLVEEVARVTSLSQLKPQP
LPREPGVPAPVLTPMQKRERSARRTMAALGYNECVTYSFIDKASAELFGGGTDAVMLENPISSEMSHMRPAVLPGLLQAA
ARNQARGMMDMALFEVGAGFHGGEPGEQHLLASGLLIGHTGPRDPHGARRPVDVYDVKADAEAVLSAIGAPAKAQILRGA
REWWHPGRHGMICLGPKKVLGIFGEVHPKVLAAMDIKGPAMAFTIFPQEVPLPRSTNASRGAVTMSDLQAVERDFAFVVP
SDVPALDLVNAAAGADKTLIADVRVFDEFIGGSLGEGNKSIALAVRMQPQDKTLTEEEIEAVADKIVTKVSKATGGVLRG

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=819, Percent_Identity=34.6764346764347, Blast_Score=436, Evalue=1e-123,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 85720; Mature: 85720

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTLSWLKDHLDTDASVTEITDALTDLGLEVEGVEDRAEALKTFTIAHIQSAEQHPDAD
CCEEHHHHHHHCCCCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCC
RLRICQVETNDGLQQIICGAPNARAGINVVLAKPGDYIPGLDITIGVGKIRGVESFGMMA
CEEEEEEECCCCHHHHHCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCHHHHCCCC
SEKELLLSDEHNGIIELKDAQVGQKFIDWLAKHDPAKVDPVIEIAITPNRQDALGIRGIA
CCCHHEEECCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHH
RDLSARGVGTLKAHDPAEIKGTYPSPISITIDADTLDTAPHFTGRLIKGVKNGPSPAWLQ
HHHHHCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHH
DRLRAIGLRPISALVDITNYFTFDQNRPLHVFDADKVAGGLRVHKAAGGEEIVALDDKTY
HHHHHHCCCHHHHHHHHHHHEEECCCCCEEEEEHHHHCCCEEEEECCCCCEEEEECCCEE
TLPEGHMVISDDTGVESIAGIMGGAATGCTEDTVNVFLESAWWDTVAIAYAGRALKINSD
ECCCCCEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEHEECCCEEEECCC
ARYRFERGVDPDYTLPGLHAATQMVLDLCGGEASEVVEAGKPLDTARSYTLRPGRVKSLV
CCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCHHCCCCCCCCCHHHHHH
GMDVAREEQARILTALGFSATGTDTLEVWVPSWRRDVQGEADLVEEVARVTSLSQLKPQP
HCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHCCCCHHHHHHHHHHHHHHHHCCCCC
LPREPGVPAPVLTPMQKRERSARRTMAALGYNECVTYSFIDKASAELFGGGTDAVMLENP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHEEEHHHHHCCHHHCCCCCCEEEECCC
ISSEMSHMRPAVLPGLLQAAARNQARGMMDMALFEVGAGFHGGEPGEQHLLASGLLIGHT
HHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHCCEEEECC
GPRDPHGARRPVDVYDVKADAEAVLSAIGAPAKAQILRGAREWWHPGRHGMICLGPKKVL
CCCCCCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEECHHHHH
GIFGEVHPKVLAAMDIKGPAMAFTIFPQEVPLPRSTNASRGAVTMSDLQAVERDFAFVVP
HHHHHHCCHHEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHCCEEEEC
SDVPALDLVNAAAGADKTLIADVRVFDEFIGGSLGEGNKSIALAVRMQPQDKTLTEEEIE
CCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCHHHHH
AVADKIVTKVSKATGGVLRG
HHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKFTLSWLKDHLDTDASVTEITDALTDLGLEVEGVEDRAEALKTFTIAHIQSAEQHPDAD
CCEEHHHHHHHCCCCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCC
RLRICQVETNDGLQQIICGAPNARAGINVVLAKPGDYIPGLDITIGVGKIRGVESFGMMA
CEEEEEEECCCCHHHHHCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCHHHHCCCC
SEKELLLSDEHNGIIELKDAQVGQKFIDWLAKHDPAKVDPVIEIAITPNRQDALGIRGIA
CCCHHEEECCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHH
RDLSARGVGTLKAHDPAEIKGTYPSPISITIDADTLDTAPHFTGRLIKGVKNGPSPAWLQ
HHHHHCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHH
DRLRAIGLRPISALVDITNYFTFDQNRPLHVFDADKVAGGLRVHKAAGGEEIVALDDKTY
HHHHHHCCCHHHHHHHHHHHEEECCCCCEEEEEHHHHCCCEEEEECCCCCEEEEECCCEE
TLPEGHMVISDDTGVESIAGIMGGAATGCTEDTVNVFLESAWWDTVAIAYAGRALKINSD
ECCCCCEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEHEECCCEEEECCC
ARYRFERGVDPDYTLPGLHAATQMVLDLCGGEASEVVEAGKPLDTARSYTLRPGRVKSLV
CCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCHHCCCCCCCCCHHHHHH
GMDVAREEQARILTALGFSATGTDTLEVWVPSWRRDVQGEADLVEEVARVTSLSQLKPQP
HCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHCCCCHHHHHHHHHHHHHHHHCCCCC
LPREPGVPAPVLTPMQKRERSARRTMAALGYNECVTYSFIDKASAELFGGGTDAVMLENP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHEEEHHHHHCCHHHCCCCCCEEEECCC
ISSEMSHMRPAVLPGLLQAAARNQARGMMDMALFEVGAGFHGGEPGEQHLLASGLLIGHT
HHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHCCEEEECC
GPRDPHGARRPVDVYDVKADAEAVLSAIGAPAKAQILRGAREWWHPGRHGMICLGPKKVL
CCCCCCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEECHHHHH
GIFGEVHPKVLAAMDIKGPAMAFTIFPQEVPLPRSTNASRGAVTMSDLQAVERDFAFVVP
HHHHHHCCHHEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHCCEEEEC
SDVPALDLVNAAAGADKTLIADVRVFDEFIGGSLGEGNKSIALAVRMQPQDKTLTEEEIE
CCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCHHHHH
AVADKIVTKVSKATGGVLRG
HHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA