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The map label for this gene is pheS

Identifier: 89052943

GI number: 89052943

Start: 453704

End: 454777

Strand: Direct

Name: pheS

Synonym: Jann_0452

Alternate gene names: 89052943

Gene position: 453704-454777 (Clockwise)

Preceding gene: 89052942

Following gene: 89052944

Centisome position: 10.51

GC content: 63.87

Gene sequence:

>1074_bases
ATGGACGGATTGAATGATTTGCGCGCCGGGTGGCTGGACCGGATCGGTGGGGCCACGGATGAGGCGACGCTGGAAGAGTT
CCGCGTCGCGGCCCTCGGCAAGAAGGGCGAAATCTCCCTGAAGATGCGTGAATTGGGCAAGATGACGCCCGAGGAGCGCC
AAGTGGCAGGCCCCGCGCTCAATGCCCTGAAGGACGAAGTGAACGCCGCGATTGCCGCGAAGAAAGCCGGCCTCGCAGAT
GCCGCGCTTGATGAGCGGTTGAAGGCCGAATGGCTCGACGTGACCCTGCCCGCCCGGCCCAAACGCGTGGGCTCCATCCA
CCCCGTGTCCCAGGTCACCGAAGAAGTCACCGCGATCTTCGCCGACATGGGCTTTTCGGTCGCGGAAGGCCCGCAGATCG
AGACGGATTTCTACAATTTCGACGCGCTCAACATTCCCGGCCATCACCCCGCGCGGGCGGAGATGGATACGTTCTACACC
CACCGCGCCGAGGGCGATAACCGCCCGCCCCACGTCTTGCGCACCCACACGTCGCCGGTGCAGATCCGCCATATGGAAAA
GCACGGCGCGCCCTGCCGCATTATCGCGCCGGGCCGCGTGTACCGCGCCGACTACGACCAGACCCACACGCCGATGTTCC
ATCAGGTGGAGGGGTTGGTTCTGGGCCGCGACGTCTCCATGGCGAACCTGAAATGGGTGCTGGAGGAGTTCTACTCCGCG
TTCTTTGGCGTGCAGGTCAAAACCCGCTTCCGCGCGTCGCACTTCCCTTTCGTGGAACCGGGCGCCGAGGTCGACATTCA
GTGCTCGTTCGAGGGCGGCACCGTGAAGGTCGGTGAGGGCGACGATTGGCTGGAGATTTTGGGTTCAGGTATGGTCCACC
CCAAGGTGCTGGAAGCCGGTGGCATCGACCCGCAGGAGTTCCAGGGCTTCGCCTTCGGCATGGGCATCGACCGCATCGCC
ATGCTGAAATACGGCATCCCCGATTTGCGTGACTTCTTCGCCAGCGACCTGCGGTGGCTACGCCACTACGGGTTTGCCTC
GTTGGAGATGCCAAGCGTGCATGCGGGGGATTGA

Upstream 100 bases:

>100_bases
AACGGGGCGCGCTTTTGCTGTTTTGGGGGATCTGCCCTCAGGTTGCATTCGGGTGGGGCTGTGATAGCTCCACGCCTGAA
ACGGGTAAGGGTCTGACGGT

Downstream 100 bases:

>100_bases
GGGGTGACGACCCAACGGCAAATTGACAGATTGTTACTGATTGTTCAGGCGTCTGATGAGGAAACAATTCGTGCCCGCTC
TGTCTTCCTTGAAGACCTGT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MDGLNDLRAGWLDRIGGATDEATLEEFRVAALGKKGEISLKMRELGKMTPEERQVAGPALNALKDEVNAAIAAKKAGLAD
AALDERLKAEWLDVTLPARPKRVGSIHPVSQVTEEVTAIFADMGFSVAEGPQIETDFYNFDALNIPGHHPARAEMDTFYT
HRAEGDNRPPHVLRTHTSPVQIRHMEKHGAPCRIIAPGRVYRADYDQTHTPMFHQVEGLVLGRDVSMANLKWVLEEFYSA
FFGVQVKTRFRASHFPFVEPGAEVDIQCSFEGGTVKVGEGDDWLEILGSGMVHPKVLEAGGIDPQEFQGFAFGMGIDRIA
MLKYGIPDLRDFFASDLRWLRHYGFASLEMPSVHAGD

Sequences:

>Translated_357_residues
MDGLNDLRAGWLDRIGGATDEATLEEFRVAALGKKGEISLKMRELGKMTPEERQVAGPALNALKDEVNAAIAAKKAGLAD
AALDERLKAEWLDVTLPARPKRVGSIHPVSQVTEEVTAIFADMGFSVAEGPQIETDFYNFDALNIPGHHPARAEMDTFYT
HRAEGDNRPPHVLRTHTSPVQIRHMEKHGAPCRIIAPGRVYRADYDQTHTPMFHQVEGLVLGRDVSMANLKWVLEEFYSA
FFGVQVKTRFRASHFPFVEPGAEVDIQCSFEGGTVKVGEGDDWLEILGSGMVHPKVLEAGGIDPQEFQGFAFGMGIDRIA
MLKYGIPDLRDFFASDLRWLRHYGFASLEMPSVHAGD
>Mature_357_residues
MDGLNDLRAGWLDRIGGATDEATLEEFRVAALGKKGEISLKMRELGKMTPEERQVAGPALNALKDEVNAAIAAKKAGLAD
AALDERLKAEWLDVTLPARPKRVGSIHPVSQVTEEVTAIFADMGFSVAEGPQIETDFYNFDALNIPGHHPARAEMDTFYT
HRAEGDNRPPHVLRTHTSPVQIRHMEKHGAPCRIIAPGRVYRADYDQTHTPMFHQVEGLVLGRDVSMANLKWVLEEFYSA
FFGVQVKTRFRASHFPFVEPGAEVDIQCSFEGGTVKVGEGDDWLEILGSGMVHPKVLEAGGIDPQEFQGFAFGMGIDRIA
MLKYGIPDLRDFFASDLRWLRHYGFASLEMPSVHAGD

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=270, Percent_Identity=31.1111111111111, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI5729820, Length=287, Percent_Identity=28.5714285714286, Blast_Score=108, Evalue=7e-24,
Organism=Escherichia coli, GI1788007, Length=343, Percent_Identity=50.1457725947522, Blast_Score=348, Evalue=3e-97,
Organism=Caenorhabditis elegans, GI17508957, Length=272, Percent_Identity=30.8823529411765, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32563657, Length=272, Percent_Identity=30.8823529411765, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32566635, Length=263, Percent_Identity=31.1787072243346, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6321087, Length=268, Percent_Identity=31.7164179104478, Blast_Score=129, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6325304, Length=349, Percent_Identity=25.5014326647564, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI18858079, Length=357, Percent_Identity=28.0112044817927, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17137424, Length=302, Percent_Identity=25.8278145695364, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_JANSC (Q28V93)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508394.1
- ProteinModelPortal:   Q28V93
- SMR:   Q28V93
- STRING:   Q28V93
- GeneID:   3932893
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0452
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   Q28V93
- ProtClustDB:   PRK00488
- BioCyc:   JSP290400:JANN_0452-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 39512; Mature: 39512

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGLNDLRAGWLDRIGGATDEATLEEFRVAALGKKGEISLKMRELGKMTPEERQVAGPAL
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCHHHHHCCHHH
NALKDEVNAAIAAKKAGLADAALDERLKAEWLDVTLPARPKRVGSIHPVSQVTEEVTAIF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHH
ADMGFSVAEGPQIETDFYNFDALNIPGHHPARAEMDTFYTHRAEGDNRPPHVLRTHTSPV
HHCCCCCCCCCCCCCCEECCEEECCCCCCCCCCHHHHHHEECCCCCCCCCCEEEECCCCE
QIRHMEKHGAPCRIIAPGRVYRADYDQTHTPMFHQVEGLVLGRDVSMANLKWVLEEFYSA
EEEEHHHCCCCEEEECCCEEEECCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHH
FFGVQVKTRFRASHFPFVEPGAEVDIQCSFEGGTVKVGEGDDWLEILGSGMVHPKVLEAG
HHCCEEEEEECCCCCCCCCCCCCEEEEEEECCCEEEECCCCHHHHHHCCCCCCCHHHHCC
GIDPQEFQGFAFGMGIDRIAMLKYGIPDLRDFFASDLRWLRHYGFASLEMPSVHAGD
CCCHHHHCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCC
>Mature Secondary Structure
MDGLNDLRAGWLDRIGGATDEATLEEFRVAALGKKGEISLKMRELGKMTPEERQVAGPAL
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCHHHHHCCHHH
NALKDEVNAAIAAKKAGLADAALDERLKAEWLDVTLPARPKRVGSIHPVSQVTEEVTAIF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHH
ADMGFSVAEGPQIETDFYNFDALNIPGHHPARAEMDTFYTHRAEGDNRPPHVLRTHTSPV
HHCCCCCCCCCCCCCCEECCEEECCCCCCCCCCHHHHHHEECCCCCCCCCCEEEECCCCE
QIRHMEKHGAPCRIIAPGRVYRADYDQTHTPMFHQVEGLVLGRDVSMANLKWVLEEFYSA
EEEEHHHCCCCEEEECCCEEEECCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHH
FFGVQVKTRFRASHFPFVEPGAEVDIQCSFEGGTVKVGEGDDWLEILGSGMVHPKVLEAG
HHCCEEEEEECCCCCCCCCCCCCEEEEEEECCCEEEECCCCHHHHHHCCCCCCCHHHHCC
GIDPQEFQGFAFGMGIDRIAMLKYGIPDLRDFFASDLRWLRHYGFASLEMPSVHAGD
CCCHHHHCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA