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The map label for this gene is pyk [H]

Identifier: 89052938

GI number: 89052938

Start: 450520

End: 451968

Strand: Direct

Name: pyk [H]

Synonym: Jann_0447

Alternate gene names: 89052938

Gene position: 450520-451968 (Clockwise)

Preceding gene: 89052935

Following gene: 89052939

Centisome position: 10.43

GC content: 61.97

Gene sequence:

>1449_bases
ATGAAACGCGACCGTAACGTCAAGATCGTTGCAACCCTCGGCCCCGCGTCCGATGACTATGAGATGATCCGGGCGCTCCA
TGAGGCCGGTGCAGACGTGTTTCGCCTAAATATGTCTCATGGGGACCATTCCGAGATTGCCGTGCGCCACGGTATCATTC
GCCAGATTGAGGCCGATCTGGGTCAACCGATTGCCATTCTGGCAGATTTGCAGGGTCCCAAGCTGCGCGTCGGCGTCTTT
GACAACGATGACGGCTACGATCTGGACGTTGGCGCGACATTCCGCATGGACCTCGATGACACGCCTGGCGACACCACCCG
CGTGCAACTGCCCCACACCGAGATTTTCGCGGCTCTGGAGCCCGGCGCGCATCTGCTGATCAACGATGGCAAGATCCGGG
TGAAGGTGCTGGAGTGCGGTACGGATTATGCCAATTGCGAAGTCCTCGCGGGTGGGCGGATTTCCAACCGCAAGGGCGTG
AACGTGCCGGACGTTGTTCTGCCGCTTGCCGCGCTCTCAGAGAAGGATCGCAAGGACCTGGAATTTGTTTGTGAGTTGGG
GGTCGACTGGTTGGCGCTGTCGTTTGTGCAGCGCCGCGAGGATGTGGACGAAGCGCGGAGCCTGGCCAAGGGCCGCGCGG
CGGTTCTGTCGAAGATCGAGAAGCCGTCTGCCGTAGCGAACTTCGGTGAAATCCTGGAGGCGTCCGACGGCATCATGGTC
GCGCGTGGCGATCTGGGTGTGGAGCTGCCCGTGCAGAACGTGCCGCCGATCCAGAAGCTTCTGGTGCGCAAGTGCCGCAA
CGCGGCCAAGCCGGTGATCGTTGCCACGCAAATGCTGGAGAGCATGATTGAAAGCCCGATGCCCACCCGCGCCGAGGTCT
CGGACGTGGCTGCCGCGATCTATGAAGGCGCCGATGCCATCATGCTCAGCGCCGAATCTGCCGCCGGTGATTTTCCGATT
GAGGCCGTGACCACGATGAACAACGTCGCCGTGGAGGTTGAAAGTGACCCCACCTACCGCAGCATCATCGAGGCCAGCCG
CGGCGGCGAGAAGGAGACGGTTGCCGATGCCATCGTATCTGCCGCGCGCGAAATCGCGGAGACGACCAACATCAAGTTGA
TCTGTTGCTTCTCGGAATCCGGCTCCACCGCCGCGCTCACGGCGCGTGAGCGGCCAAATGTGCCGATCCTCGCCCTGACC
TCGCGCCAGGCCACGGCGCGGCGGATGTGCCTGACCTGGGGTTGCCATTGTGTGAAGGTCGGTTCGGTCGGACGTTTCAA
GGAAGCGGTGATCAGCGCCGTGCGCGCGGCCACCACGGAAGGGCTGGCAGAAACGACAGATCAGATCGTCGTGACCGCGG
GTGTGCCGTTTGGTCAATCGGGCTCCACCAACATTCTGCGGATTGCACCCTGCGATGAGCGGTTGATTTACGCGTCCGAG
CTGGAATGA

Upstream 100 bases:

>100_bases
CAATAGAGGTGCAACGGCGCGACTGATAGCGCAAACATCGTCGTAAATGCGCGATGATCCCGATACCCAAGACCCAGACT
GGCAAGAGGATGACAGATCC

Downstream 100 bases:

>100_bases
CCCTGCCGGTCGGCGCAGACATCCTTCTGGTGATCGCCACCGTGGTCGGGATCTTGTCGCTCTCGTCCATTGTGGCCGCT
TGGACGATCAAACGCTGGCC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKRDRNVKIVATLGPASDDYEMIRALHEAGADVFRLNMSHGDHSEIAVRHGIIRQIEADLGQPIAILADLQGPKLRVGVF
DNDDGYDLDVGATFRMDLDDTPGDTTRVQLPHTEIFAALEPGAHLLINDGKIRVKVLECGTDYANCEVLAGGRISNRKGV
NVPDVVLPLAALSEKDRKDLEFVCELGVDWLALSFVQRREDVDEARSLAKGRAAVLSKIEKPSAVANFGEILEASDGIMV
ARGDLGVELPVQNVPPIQKLLVRKCRNAAKPVIVATQMLESMIESPMPTRAEVSDVAAAIYEGADAIMLSAESAAGDFPI
EAVTTMNNVAVEVESDPTYRSIIEASRGGEKETVADAIVSAAREIAETTNIKLICCFSESGSTAALTARERPNVPILALT
SRQATARRMCLTWGCHCVKVGSVGRFKEAVISAVRAATTEGLAETTDQIVVTAGVPFGQSGSTNILRIAPCDERLIYASE
LE

Sequences:

>Translated_482_residues
MKRDRNVKIVATLGPASDDYEMIRALHEAGADVFRLNMSHGDHSEIAVRHGIIRQIEADLGQPIAILADLQGPKLRVGVF
DNDDGYDLDVGATFRMDLDDTPGDTTRVQLPHTEIFAALEPGAHLLINDGKIRVKVLECGTDYANCEVLAGGRISNRKGV
NVPDVVLPLAALSEKDRKDLEFVCELGVDWLALSFVQRREDVDEARSLAKGRAAVLSKIEKPSAVANFGEILEASDGIMV
ARGDLGVELPVQNVPPIQKLLVRKCRNAAKPVIVATQMLESMIESPMPTRAEVSDVAAAIYEGADAIMLSAESAAGDFPI
EAVTTMNNVAVEVESDPTYRSIIEASRGGEKETVADAIVSAAREIAETTNIKLICCFSESGSTAALTARERPNVPILALT
SRQATARRMCLTWGCHCVKVGSVGRFKEAVISAVRAATTEGLAETTDQIVVTAGVPFGQSGSTNILRIAPCDERLIYASE
LE
>Mature_482_residues
MKRDRNVKIVATLGPASDDYEMIRALHEAGADVFRLNMSHGDHSEIAVRHGIIRQIEADLGQPIAILADLQGPKLRVGVF
DNDDGYDLDVGATFRMDLDDTPGDTTRVQLPHTEIFAALEPGAHLLINDGKIRVKVLECGTDYANCEVLAGGRISNRKGV
NVPDVVLPLAALSEKDRKDLEFVCELGVDWLALSFVQRREDVDEARSLAKGRAAVLSKIEKPSAVANFGEILEASDGIMV
ARGDLGVELPVQNVPPIQKLLVRKCRNAAKPVIVATQMLESMIESPMPTRAEVSDVAAAIYEGADAIMLSAESAAGDFPI
EAVTTMNNVAVEVESDPTYRSIIEASRGGEKETVADAIVSAAREIAETTNIKLICCFSESGSTAALTARERPNVPILALT
SRQATARRMCLTWGCHCVKVGSVGRFKEAVISAVRAATTEGLAETTDQIVVTAGVPFGQSGSTNILRIAPCDERLIYASE
LE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=490, Percent_Identity=37.7551020408163, Blast_Score=282, Evalue=5e-76,
Organism=Homo sapiens, GI33286420, Length=490, Percent_Identity=37.7551020408163, Blast_Score=282, Evalue=5e-76,
Organism=Homo sapiens, GI33286418, Length=488, Percent_Identity=37.7049180327869, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI32967597, Length=489, Percent_Identity=35.9918200408998, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI10835121, Length=489, Percent_Identity=35.9918200408998, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=38.3275261324042, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=38.3275261324042, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=33.6170212765957, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=33.6170212765957, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI310128738, Length=212, Percent_Identity=33.0188679245283, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1787965, Length=467, Percent_Identity=39.186295503212, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=38.9121338912134, Blast_Score=286, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17544584, Length=489, Percent_Identity=35.9918200408998, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI71984406, Length=482, Percent_Identity=35.0622406639004, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI71984413, Length=482, Percent_Identity=35.0622406639004, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17506831, Length=482, Percent_Identity=35.0622406639004, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17506829, Length=482, Percent_Identity=35.0622406639004, Blast_Score=239, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6324923, Length=483, Percent_Identity=34.9896480331263, Blast_Score=243, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6319279, Length=420, Percent_Identity=37.1428571428571, Blast_Score=241, Evalue=2e-64,
Organism=Drosophila melanogaster, GI28571814, Length=481, Percent_Identity=36.7983367983368, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24648964, Length=481, Percent_Identity=36.7983367983368, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24581235, Length=426, Percent_Identity=35.9154929577465, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24648966, Length=400, Percent_Identity=35, Blast_Score=222, Evalue=4e-58,
Organism=Drosophila melanogaster, GI24646914, Length=249, Percent_Identity=37.7510040160643, Blast_Score=151, Evalue=1e-36,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51743; Mature: 51743

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRDRNVKIVATLGPASDDYEMIRALHEAGADVFRLNMSHGDHSEIAVRHGIIRQIEADL
CCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHC
GQPIAILADLQGPKLRVGVFDNDDGYDLDVGATFRMDLDDTPGDTTRVQLPHTEIFAALE
CCCEEEEEECCCCEEEEEEEECCCCEEEECCCEEEECCCCCCCCCEEEECCHHHEEEEEC
PGAHLLINDGKIRVKVLECGTDYANCEVLAGGRISNRKGVNVPDVVLPLAALSEKDRKDL
CCCEEEEECCEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCHHHH
EFVCELGVDWLALSFVQRREDVDEARSLAKGRAAVLSKIEKPSAVANFGEILEASDGIMV
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEE
ARGDLGVELPVQNVPPIQKLLVRKCRNAAKPVIVATQMLESMIESPMPTRAEVSDVAAAI
EECCCCEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
YEGADAIMLSAESAAGDFPIEAVTTMNNVAVEVESDPTYRSIIEASRGGEKETVADAIVS
HCCCCEEEEEECCCCCCCCCCEEEECCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHH
AAREIAETTNIKLICCFSESGSTAALTARERPNVPILALTSRQATARRMCLTWGCHCVKV
HHHHHHHHCCEEEEEEECCCCCEEEEEECCCCCCCEEEEECCCHHHHHHHHHHCCEEEEE
GSVGRFKEAVISAVRAATTEGLAETTDQIVVTAGVPFGQSGSTNILRIAPCDERLIYASE
CCCCHHHHHHHHHHHHHHHCCHHHCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEEC
LE
CC
>Mature Secondary Structure
MKRDRNVKIVATLGPASDDYEMIRALHEAGADVFRLNMSHGDHSEIAVRHGIIRQIEADL
CCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHC
GQPIAILADLQGPKLRVGVFDNDDGYDLDVGATFRMDLDDTPGDTTRVQLPHTEIFAALE
CCCEEEEEECCCCEEEEEEEECCCCEEEECCCEEEECCCCCCCCCEEEECCHHHEEEEEC
PGAHLLINDGKIRVKVLECGTDYANCEVLAGGRISNRKGVNVPDVVLPLAALSEKDRKDL
CCCEEEEECCEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCHHHH
EFVCELGVDWLALSFVQRREDVDEARSLAKGRAAVLSKIEKPSAVANFGEILEASDGIMV
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEE
ARGDLGVELPVQNVPPIQKLLVRKCRNAAKPVIVATQMLESMIESPMPTRAEVSDVAAAI
EECCCCEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
YEGADAIMLSAESAAGDFPIEAVTTMNNVAVEVESDPTYRSIIEASRGGEKETVADAIVS
HCCCCEEEEEECCCCCCCCCCEEEECCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHH
AAREIAETTNIKLICCFSESGSTAALTARERPNVPILALTSRQATARRMCLTWGCHCVKV
HHHHHHHHCCEEEEEEECCCCCEEEEEECCCCCCCEEEEECCCHHHHHHHHHHCCEEEEE
GSVGRFKEAVISAVRAATTEGLAETTDQIVVTAGVPFGQSGSTNILRIAPCDERLIYASE
CCCCHHHHHHHHHHHHHHHCCHHHCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEEC
LE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA